Digital Commons Network^™

Implications Of Antibiotic And Bacteriophage Resistance In Environmentally Isolated E. Coli, Michael Connolly

Honors Theses

The increasing prevalence of antibiotic-resistant bacteria is an emerging problem for humans. Clinical misuse, overuse in agricultural and food settings, and limited numbers of new antibiotics have accelerated the proliferation of antibiotic-resistant bacteria. To confront this threat, scientists must develop new therapeutics that kill these antibiotic-resistant bacteria. In this study, we used Escherichia coli to analyze antibiotic and bacteriophage susceptibility. E. coli is a common, mostly benign, enteric, gram-negative bacteria. We isolated three E. coli strains from the Hans Groot Kill, a stream that runs through Union College’s campus. We sought to assess various E. coli strains’ antibiotic resistance, susceptibility …

Investigating The Role Of The Cholesterol Recognition/Interaction Amino-Acid Consensus Sequence In Follicle Stimulating Hormone Receptor Function And Structure, Tatyana Lynn

Honors Theses

Human infertility is a complex disorder that can often be attributed to a dysfunction of the endocrine system. Follicle-stimulating hormone (FSH) is one of many hormones that participate in a complex process in both women and men to regulate normal reproduction. The dysfunction of this hormone and its receptor are some of the many causes of infertility. FSH is secreted by the anterior pituitary and, in women, initiates a cascade of biological events that enable ovulation. FSH carries out its function by binding and activating specific receptors. The FSH receptor (FSHR) is a G protein-coupled receptor (GPCR) that is located …

Cortisol Receptor Sensitivity As A Risk Factor For Depression, Michela Michielli

Honors Theses

In 2020, the World Health Organization reported over 264 million people across the world were suffering from depression. Studies have demonstrated that one source of depression is a hormonal imbalance involved in the stress response. Cortisol is a stress hormone regulated by the Hypothalamic-Anterior-Pituitary (HPA) Axis. Its effects on the stress response and other metabolic activities in the body are exerted through the glucocorticoid and mineralocorticoid receptors (GR and MR respectively).

Our research has examined mutations known as single-nucleotide-polymorphisms (SNPs) relating to cortisol-receptor sensitivity and the behavior of cortisol in the body to investigate the link between cortisol activity and …

Lipid Raft Disruption Alters Human Follicle Stimulating Hormone Receptor Signaling, Rachel Judith Godek

Honors Theses

Over 6.7 million people struggle with infertility each year. Studying signaling by reproductive hormones in fertility can allow us to gain a better understanding of the signaling pathways that must function correctly for proper fertility. Some infertility is due to incorrect human follicle stimulating hormone receptor (hFSHR) function. When follicle stimulating hormone (FSH) binds to hFSHR this begins a signaling cascade where the end product is the maturation of sperm by Sertoli cells in men, and egg development and production of estrogen through stimulation of granulosa cells in women. It has been determined that hFSHR is localized to microdomains of …

Blocking The Entry Of Hiv Into Host Cells Through Co-Receptor Inhibition, Garyfallia Ralli

Honors Theses

The goal of our project is to synthesize a dual CXCR4/CCR5 co-receptor inhibitor to block the entry of HIV into host cells. In the early phase of the HIV-1 replication cycle, HIV-1 binds to host cells through the CD4 protein present on the host cell surface. To infect the cell, HIV-1 requires further interactions that promote fusion of the viral and cellular membranes. This can occur through binding to the chemokine co-receptors such as CXCR4 and CCR5. We are choosing dual inhibition since under selective pressure of a CCR5 antagonist, CXCR4-using strains have been shown to predominate. Through dual inhibition, …

Improving An Ssvep-Based Brain Computer Interface Speller, Mac Kenzie J. Frank

Honors Theses

A brain-computer interface (BCI) is a novel technology that creates direct assistive communication between the brain and a computer. While numerous electroencephalogram (EEG) based BCI-speller applications have been used for communication by adults with physical disabilities; few BCI studies have included children, and none using BCI spellers. A pilot study of a developmentally-appropriate EEG-based speller-storybook interface that relied on steady-state visual evoked potentials (SSVEPs) by two pediatric users with quadriplegic cerebral palsy showed limited speller reliability (E. Floreani, personal communication, September 30, 2021). In the pilot study, the alphabet was parsed between three boxes, each flashing at a different rate …

Mutations In Caveolin Binding Motif Alter Human Follicle Stimulating Hormone Receptor Signaling, Katarina Zahedi

Honors Theses

Globally, there are about 48 million couples and 186 million individuals of reproductive age that are affected by infertility. Some cases of infertility in both men and women have been attributed to impaired follicle stimulating hormone (FSH) signaling. The lack of proper function of the cognate receptor for FSH (FSHR) could contribute to infertility since the biochemical signal generated by FSH binding to FSHR stimulates the production of a sperm-stabilizing protein in males and follicle maturation in females. It has been demonstrated that human FSHR (hFSHR) localizes to lipid rafts, which are rigid and detergent-resistant microdomains in the cell membrane. …

Ecoturismo Y Empoderamiento: La Ecoinnovación De Las Mujeres Indígenas En América Latina, Kathleen Sinatra

Honors Theses

This thesis focuses on the role of indigenous women in relation to ecotourism efforts and ecoinnovation. Through an analysis of three case studies, I discuss the need to account for both indigenous people and gender when engaging in ecotourism efforts. I first engage in a literature review that develops the complex relationship between the identity factors of indigeneity and gender. I then look at a case study of the hotel Taselotzin in Cuetzalan del Progreso, Mexico in which a cooperative group of indigenous women founded a hotel. I then turn to the Kichwa community in Ecuador and the organization Amukishmi …

The Role Of Single Nucleotide Polymorphisms Causing A Dysregulation Of The Hpa Axis On The Incidence Of Depression, Elsa Bechu, Brian Cohen

Honors Theses

Depression is one of the most prevalent diseases worldwide, afflicting approximately 17 million adults in the US in 2019. A prominent causal factor of depression is dysregulation of our body’s response to stress when exposed to continuous stressors over long periods of time. Stress is regulated by the Hypothalamic-Pituitary-Adrenal (HPA) axis, with cortisol as its effector hormone. The effects of cortisol are exerted through the glucocorticoid and mineralocorticoid receptors (GR and MR, respectively) in the body and the brain, respectively. The HPA axis is regulated by a negative feedback loop where activation of the GR by circulating cortisol inhibits the …

Hybrid Viability: An Analysis Of Drosophila Hybrid Competition And Mating Success Amongst Its Parental Species, Konrad Drzymalski

Honors Theses

The topic of hybrid offspring viability and mating preference between the two parental species of Drosophila athabasca (West Northern) and Drosophila mahican (Eastern A) is a still largely unexplored avenue of study. While the two aforementioned parental Drosophila species exist in distinct geographical regions across North America, they occupy a sympatric zone in the North East. Due to the absence of hybrid offspring in nature and the high levels of sexual isolation between these two parental species, it is necessary to establish if, and how hybrid offspring suffer in nature. By utilizing no choice mating systems involving audio playback of …

Analysis Of Caveolin-1 Expression And Interaction With Fshr In Lipid Raft Domains, Joseph Bott

Honors Theses

For thousands of women in the United States ovarian cysts, tumors, and hormonal imbalances contribute to reduced fertility and fecundity. In addition to these factors, many other unexplained physiological mechanisms may play a role in women’s reproductive health. Specifically, human follicle stimulating hormone (hFSH) binds to a G protein coupled receptor (GPCR) called human follicle stimulating hormone receptor (hFSHR). This interaction helps to regulate the reproductive system in both males and females indicating that it may be a key player to target in order to promote fertility. Previous work in our lab has demonstrated that hFSHR, like other GPCR, has …

The Association Among Executive Functioning, Self-Efficacy And Adhd With Attitudes Towards Online Learning, Alice Sperry

Honors Theses

Prior research has shown that worse executive function and lower self-efficacy are associated with learning differences such as ADHD and associated with psychiatric diagnoses. The purpose of this study was to investigate the interaction among executive function, self-efficacy, attitudes towards online learning and learning differences. Fifty-one participants completed a survey which assessed executive function, self-efficacy, attitudes towards online learning and also asked demographic questions. The results indicated that students with learning differences and students with a psychiatric diagnosis had significantly worse executive function than students without a diagnosis. Students with a learning difference were also found to have significantly lower …

Analysis Of Differential Gene Expression Patterns During Spiral Intestine Development In Leucoraja Erinacea, Kelsey Cox

Honors Theses

The vertebrate gut is a specialized structure responsible for the intake and digestion of food, absorption of nutrients, and expulsion of waste products. The organs of the digestive tract have been conserved over time, but modifications to the size and shape of individual structures exist within the different vertebrate lineages. The skate’s spiral intestine has evolved to maximize nutrient absorption within a compact structure to create space in the body cavity for the organs needed for buoyancy. Studying the unique intestinal morphology of Leucoraja erinacea, or the little skate, provides an opportunity to understand the role of differential gene expression …

Investigating Human Follicle Stimulating Hormone Receptor And Its Partners Using The Apex Assay, Alexandra Temple

Honors Theses

Many men and women deal with fertility whether it’s needing infertility treatments or contraceptives. The follicle stimulating hormone (FSH) and follicle stimulating hormone receptor (FSHR) play important roles in reproduction and fertility in both males and females. Fertility issues can arise when interactions between FSH and FSHR aren’t functioning properly and FSHR signaling is disrupted. FSHR is a G-protein coupled receptor (GPCR) found on the cell surface of granulosa cells in women and Sertoli cells in men. Activated FSHR initiates a cascade of downstream signaling events that result in different biological effects such as ovarian follicular development and estrogen production …

Analyzing Thermal Gradients Using Terrestrial Laser Scanning In Scrub Oak Habitats, Alyssa Silbey

Honors Theses

Thermal microclimates are an important component of natural ecosystems because they provide appropriate niche space for many organisms; however, they are not widely studied because of their small scale. Likewise, variation in these microclimates may significantly impact animal thermoregulation and plant physiological processes and be especially important in the face of climate change. The goal of this study is to determine how the size and structure of Quercus ilicifolia shrubs influences ground temperature gradients. We hypothesized that differences in leaf cover and leaf area index (LAI) would cause temperature under shrubs to cool relative to the ambient temperature, and that …

Exploration Of Stable Isotope Analysis To Identify Prior Host In Ixodes Scapularis, Imogene Welles

Honors Theses

One of the most enigmatic concepts in tick-borne disease ecology is how to identify the prior host of a questing tick. The ability to do so would provide predictions to directly aid in controlling the spread of the many tick-borne pathogens, including the bacterial spirochete Borrelia burgdorferi, which causes Lyme disease in humans. I explored the application of a novel technique, stable isotope analysis (SIA), to identify the most recent host in molted Ixodes scapularis (black-legged tick). The common reservoir and feeding host, Peromyscus leucopus (white-footed mice; n = 46), were trapped, infested with nymphal ticks, and fed restricted …

Investigation Of Even-Skipped, A Developmentally-Regulated Gene Controlling Neural Segmentation In Dragonflies, Kathryn Bangser

Honors Theses

A comprehensive understanding of the genetic mechanisms underlying pattern formation and neurogenesis is necessary in order to trace the evolutionary history of insect embryogenesis.

One of the most important processes of embryogenesis is the organized pattern formation that allows for proper body segmentation and neural development. Proper segmentation, which relies on a series of specific gene expressions, is necessary for the development of an operational nervous system. Even-skipped (eve), one such regulatory gene, has been studied extensively in certain model organisms, and theories regarding the evolution of its functional role could be further elucidated by visualizing its expression …

Mutations In The Human Follicle Stimulating Hormone Receptor Caveolin Interaction Motif Cause Increased Basal Activation, Elizabeth Altman

Honors Theses

Over twelve percent of women aged fifteen to forty-five in America suffer from infertility and/or impaired fecundity and over seven million women have used infertility services, such as intrauterine insemination and in vitro fertilization. Some cases of infertility may be due to dysfunctional human follicle stimulating hormone (hFSH) signaling. hFSH plays a role in spermatogenesis in males, as well as follicle maturation and estrogen production in females. Problems with either hFSH or the hFSH receptor (hFSHR) decrease fertility in males and cause complete infertility in females. As part of the hypothalamic-pituitary-gonadal axis, hFSH is released from the pituitary gland and …

How To Combat Post-Natural Disaster Related Environmental Gentrification And Environmental Inequality Accelerated By Climate Change, Marielle Christie

Honors Theses

This thesis aims to illustrate the concept of natural disaster-induced environmental gentrification. There is a heightened vulnerability to unmitigated forced displacement by socioeconomically disadvantaged residents following a natural disaster. Environmental gentrification is a variant of green gentrification. Green gentrification occurs when providing green amenities to a city increases local property values and attracts wealthier residents to a previously polluted or disenfranchised neighborhood, which displaces the low-income residents. Similarly, environmental gentrification is a process that occurs after a natural disaster and rapidly accelerates the process of traditional urban gentrification or green gentrification. As a result, environmental gentrification magnifies and facilitates further …

Light-Induced Expression Of A Blue Coral Protein In An Industrial Fungus, Lindsay Tomczak

Honors Theses

The ultimate goal of this research project is to improve the growth and structural characteristics of an Ecovative Design LLC (Green Island, NY) production strain to produce commercial biomaterials for packaging. These biomaterials are produced from renewable resources and can be easily broken down after they fulfill their purpose, unlike the commonly used materials today (such as Styrofoam). In an effort to quantify the light-reactivity of the fungus, a codon-optimized DNA sequence coding a blue chromoprotein was introduced and utilized as a visual reporter gene. Transcriptional controlling sequences were identified from orthologs to specific light-regulated genes and were combined with …

Nature Abounds: Term Paper, Ricky Verre

Honors Theses

Nature Abounds is a non-profit organization that is working internationally to spread the word about climate change and all of its effects that we are currently experiencing. Climate change is one of the most pressing issues of our time and any chance at reversing its erratic effects requires immediate action. Nature Abounds employs numerous programs that serve to increase the general public's knowledge of the climate related issues at hand, as well as to have a positive environmental impact in order to increase global environmental health. The organization has programs that focus on biodiversity, water quality monitoring, forest preservation, and …

The Exploration Of Neuronal Responses To Auditory Stimuli In The Dragonflies, Anax Junius And Aeshna Constricta, Brian Shaw

Honors Theses

To date there is no published evidence that dragonflies (Odonata), have a nervous system equipped to process auditory stimuli. Even with considerable research on these creatures due to their specialized vision and flight mechanics, there is no evidence that dragonflies have ears or even auditory neurons. Last year student Andrew Hamlin and Professor Robert Olberg recorded neuronal responses in the dragonfly to auditory stimuli of 100-2000Hz sounds (Olberg and Hamlin, unpublished). This year our research was aimed at understanding a sensory modality that was previously unknown in dragonflies, the sense of hearing. In order to investigate this question …

Can An Invasive Species Burn Soil Carbon? Black Locust Invasion And Soil Carbon In The Albany Pine Bush, Jacqueline Sharry

Honors Theses

Invasive plant species can have dramatic and pervasive impacts on ecosystems, from species interactions, to resource availability, to biodiversity. One such invasive plant, the nitrogen-fixing black locust tree (Robinia pseudoacacia), has had significant effects on the soil chemistry in the globally rare inland pine barren ecosystem of the Albany Pine Bush. We have documented a loss of soil carbon following invasion by black locust, one that persists long after locusts are removed. We hypothesize that the nitrogen added through fixation by the black locust increases the carbon flux from soil to the atmosphere. Because decomposition rates are dependent …

Biological Soil Crusts In A Northeastern Pine Barren: Composition And Ecological Effects, Jessica Gilbert

Honors Theses

Biological soil crusts (BSCs), otherwise known as cryptogamic soil crusts, biocrusts, or cyanobacterial crusts, are soil aggregations hosting diverse biotic communities. They are composed of cyanobacteria and algae, and generally have a covering of moss and/or lichen. BSCs are typically found in arid to semi-arid regions throughout the world, and are integral soil stabilizers, moisture retainers, and nitrogen fixers in these communities. Along with these factors, BSCs are able to impact germination and establishment of plants, either as an accompanying influence, or direct result of those listed above. BSCs have yet to be formally described in the inland northeastern United …

An Empirical Analysis Of Climatic, Geographic, And Cultural Determinants Of International Tourism, Ethan Straus

Honors Theses

Each year, billions of people visit different countries all around the world. For many of those countries, tourism is their primary industry, leading to millions of jobs and dollars in revenue. It is expected that by 2020 total International Tourism Receipts will reach 2 trillion US dollars annually. Currently, tourism employs an estimated 200 million people around the world. With the continued progression of climate change, the tourism industry is facing a newfound threat. Global temperatures and the seal level are both expected to rise significantly by the end of the century. Additionally, the Intergovernmental Panel on Climate Change has …

Color Vision And Signal Color Evolution In Anolis Lizards, Catherine S. Wadman

Honors Theses

Anolis is a diverse genus of small arboreal lizards that utilize a visual communication system using a colorful expandable throat-fan (dewlap). They signal either to attract females or repel males. The environment the lizard lives in has influenced the coloring of these dewlaps. Species that occupy shaded habitats tend to have yellow dewlaps while those that inhabit brighter habitats tend to have red dewlaps. In experiment 1 we used a color perception assay to test whether a red or yellow stimulus against a green background was more visible to the lizard under different light intensities mimicking a dewlap appearing in …

Caveolin Binding Motif Mutation Yields A Variance In Follicle Stimulating Hormone Receptor Signaling, Justin Fleischer

Honors Theses

The human follicle stimulating hormone receptor (hFSHR) is a glycoprotein hormone receptor belonging to the g protein-coupled receptor family. It is important in both male and female reproductive processes; defects in hFSHR can lead to infertility, delayed puberty, reduced muscle bulk, and osteoporosis. Work in other labs has shown that GPCRs can be localized to microdomains located within the cell membrane called lipid rafts. These regions are highly resistant to detergents because of the high concentration of sphingolipids and cholesterol. Also within these domains, an intracellular protein, caveolin, is present. Our lab has shown that hFSHR also localizes to lipid …

The Linkage Between Hpa Axis Dysregulation And Metabolic Syndrome, Brianna Godlewski

Honors Theses

A diagnosis of Metabolic Syndrome (MSX) requires patients to present with three or more of the following symptoms: elevated fasting blood glucose levels, elevated serum triglyceride levels, low serum HDL levels, elevated blood pressure, and truncal obesity. This pathology shares many similarities with Cushing’s Syndrome (CS) but a diagnosis of CS requires hypercortisolemia. This similarity has led our lab and others to hypothesize that MSX may be a Cushingoid-like state caused by hypersensitivity of the glucocorticoid receptor (GR), a nuclear hormone receptor that is activated when cortisol binds, and or hyperactivity of the enzyme 11beta-hydroxysteroid dehydrogenase (11?- HSD) type 1, …

Development Of Novel Allele Specific Pcr Based Assays To Investigate The Contribution Of Cortisol To Metabolic Syndrome And It's Potential Implications In Depression Treatment, Justin Robert Salm

Honors Theses

The shared circuitries of depression and the stress response, regulated by the Hypothalamic-Pituitary-Adrenal (HPA) axis, implies that major depression may reflect dysregulations of the HPA axis. Previous studies find that that melancholic depression is accompanied by hyperactive HPA activity, anxiety, insomnia etc. Adversely, patients with atypical depression present CRH deficiency accompanied by lethargy and fatigue. According to the corticosteroid receptor hypothesis, HPA axis dysregulation that causes these variable phenotypic depressive moods may be attributed to defective GR expression or function. Multiple GR polymorphisms that could impact its expression or function have been identified. Due to limitations, it was not feasible …

Neuropsychological Test Performance In Pediatric Tbi, Learning Disability, And Adhd: Difference In Reliable Digit Span, Emily West

Honors Theses

Children have been found to give non-credible performances on neuropsychological evaluations and using Performance Validity Tests (PVT) can help identify such cases, but maximum effort is necessary for tests to be reliable for determining next steps. This study assessed the differences in rates of non-credible performance on the Reliable Digit Span (RDS) between traumatic brain injury (TBI), learning disability (LD) and ADHD diagnoses in a pediatric clinical sample. It was hypothesized that those with ADHD would have a higher failure rate than those with either TBI or LD. RDS data from 200 clients referred to neuropsychological testing agencies were collected …