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Life Sciences

Louisiana State University

Theses/Dissertations

2014

Resistance

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Screening For Resistance To Sugarcane Brown Rust With Controlled Conditions Inoculation, Mavir Carolina Avellaneda Barbosa Jan 2014

Screening For Resistance To Sugarcane Brown Rust With Controlled Conditions Inoculation, Mavir Carolina Avellaneda Barbosa

LSU Master's Theses

Brown rust, caused by Puccinia melanocephala, is an important disease of sugarcane. Breeding for host plant resistance is the primary control measure. Screening for resistance has relied on rating the severity of symptoms caused by natural infection; however, erratic results make this method problematic. A method accomplishing both infection and disease expression under controlled conditions could avoid the problems associated with resistance evaluations under natural infection. Inoculation of seedlings was evaluated to determine whether it could provide accurate resistance ratings in cross appraisal, and inoculation under controlled conditions was evaluated for the potential to accurately determine resistance reactions in clones …


Resistance Of Fall Armyworm, Spodoptera Frugiperda (J.E. Smith) To Bacillus Thuringiensis, Ying Niu Jan 2014

Resistance Of Fall Armyworm, Spodoptera Frugiperda (J.E. Smith) To Bacillus Thuringiensis, Ying Niu

LSU Master's Theses

The fall armyworm, Spodoptera frugiperda (J.E. Smith), is a target pest of Bacillus thuringiensis (Bt) corn in North and South America. In this study, multiple tests were conducted in the laboratory and greenhouse to 1) determine the susceptibility of two field populations of S. frugiperda collected from Florida (FL) and Puerto Rico (PR) to purified Cry1F protein and Bt corn leaf tissue and 2) evaluate larval survival of and plant injury by Cry1F-susceptible, -resistant, and –heterozygous genotypes of S. frugiperda on whole plants of transgenic corn containing single and pyramided Bt genes. Corn hybrids evaluated in this study included five …