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Fine Structure And Organ Culture Of Chick Embryo Dorsal Aorta, C. Ward Kischer, Michael Jaqua

Scanning Microscopy

Embryonic blood vessels have not been grown in organ culture, in a way which might easily submit them to studies of vascular organogenesis. The chick embryo dorsal aorta is easily accessible and relatively simple to explant to culture. Its organ culture may provide a model for wounding and repair of the intima and/or media and provide a model for studies of growth (or maintenance) and differentiation. Fresh dorsal aorta and its organ culture from the chick embryo was characterized morphologically by light microscopy scanning (SEM) and transmission electron microscopy (TEM) from days 4 to 18. Explants were incubated up to …

Investigation Of Surfaces And Interfaces By Electron Optical Methods, H. Seiler

Scanning Microscopy

The combination of methods for surface analysis with electron microscopes (EM) gives the possibility for surface and interface microanalysis. The paper deals with different methods a) for imaging of surfaces with high lateral resolution: Emission EM (EEM), Scanning EM (SEM), Reflection EM (REM) Transmission EM (TEM) using special preparation methods as replica techniques or cross section specimens b) for crystal structure investigation: Low and High Energy Electron Diffraction and Electron Chanelling Patterns and c) for material analysis using electron spectroscopy either of the emitted or scattered electrons.

The combination of all analytical methods in one instrument for surface microanalysis (SMA) …

Changes In The Tracheal Ciliated Cells In Rabbits Treated By Cis-Diamminedichloroplatinum (Ii) As Studied By Electron Microscopy, M. Albertsson, C. -H. Hakansson

Scanning Microscopy

The ciliated epithelium of the rabbit's trachea was investigated after a single 5 mg dose of cis-diamminedichloroplatinum (cis-DDP). Specimens were taken for scanning electron microscopy, transmission electron microscopy and light microscopy. Examination was performed daily for 20 consecutive days. A cytotoxic effect of the drug on the ciliated epithelium was observed with bent ciliary tips, swollen tips and broken cilia. Finally the cilia were lost and large areas of the surface were covered with microvilli. However, 20 days after the drug injection, the restitution of the ciliary carpet was almost complete.

Structure And Function Of Connective Tissue In Cardiac Muscle: Collagen Types I And Iii In Endomysial Struts And Pericellular Fibers, Thomas F. Robinson, Leona Cohen-Gould, Stephen M. Factor, Mahboubeh Eghbali, Olga O. Blumenfeld

Scanning Microscopy

Heart myocytes and capillaries are enmeshed in a complex array of connective tissue structures arranged in several levels of organization: epimysium, the sheath of connective tissue that surrounds muscles; perimysium, which is associated with groups of cells; and endomysium, which surrounds and interconnects individual cells. The present paper is a review of work in this field with an emphasis on new, unpublished findings, including composition of endomysial fibers and disposition of newly described perimysial fibers. The role of scanning electron microscopy in the development of current understanding is also outlined. Biaxially arranged epimysial fibers form a sheath around papillary muscles …

Clinical Applications Of Scanning Electron Microscopy And Energy Dispersive X-Ray Analysis In Dermatology - An Up-Date, Bo Forslind

Scanning Microscopy

Dermatological papers comprising scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis data published 1983 through 1986 in international journals are reviewed, as an update to our 1984 paper on Clinical applications of scanning electron microscopy and X-ray microanalysis in dermatology. The present paper not only deals with a review of recent publications in this area but also presents the application of microincineration to hair and cryosectioned freeze-dried skin specimens. Examples of the increased contrast obtained in hair cross sections are presented and a discussion on the feasibility of microincineration at analysis of hair and skin cross sections is …

The Effects Of Gamma Irradiation And Calcium Treatment On The Ultrastructure Of Apples And Pears, E. Kovacs, A. Keresztes, J. Kovacs

Food Structure

The post-storage quality of fruits depends on the ultrastructure of the outer tissues and on the fruit mineral composition (mostly on calcium). Pectins and their bonds with calcium play a central role in the tissues during ripening.

During senescence the middle lamella disappears from the cell walls, the fruits become soft and cell wall reticulation can be seen. There are changes within the cells too; plasmalemma separates from the wall, tonoplast from the cytoplasm, and plastids, mitochondria and nuclei are disorganized.

The ultrastructure of the skin and flesh of apples and pears were studied as a function of treatments (calcium, …

The Use Of Transmission Electron Microscopy To Study The Composition Of Pseudomonas Fragi Attachment Material, Paula J. Herald, Edmund A. Zottola

Food Structure

Electron microscopy techniques were used to study the attachment matrix of Pseudomonas fragi used to demonstrate the presence of attachment fibrils of P. fragi adhered to stainless steel. Transmission electron microscopy (TEM) was used to examine thin sectioned cells strained with ruthenium red or alcian blue. Extracellular substances surrounding cells stained with ruthenium red or alcian blue had a mat- or spike-like morphology. This indicated that these substances were acidic mucopolysaccharides that may be involved in the attachment of P. fragi to food contact surfaces.