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Life Sciences

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2016

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Analysis Of Joint Dysfunction During An Aging- Simulation, Laboratory Exercise For Allied Health Students, Anna E. O'Connor Jan 2016

Analysis Of Joint Dysfunction During An Aging- Simulation, Laboratory Exercise For Allied Health Students, Anna E. O'Connor

Honors Theses

The aim of this experiment was to (1) develop and assess a joint-dysfunction simulation learning and sensitization activity for a Human Anatomy and Physiology I lab on joint structure and function (Part A) and (2) quantify the range of motion experienced during the simulation (Part B). The lab protocol allowed 318 allied health students to experience some of the physical limitations of aging, specifically arthritis, by asking them to perform activities of daily living (ADLs) while wearing simulation equipment that inhibited their range of movement. In Part A, students completed a pre- simulation survey, which assessed the students' knowledge about …

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The Effects Of Tillage, Glyphosate, And Genetic Modification On Bacterial Root Endophyte Composition In Zea Mays, Breena Lori Nolan Jan 2016

The Effects Of Tillage, Glyphosate, And Genetic Modification On Bacterial Root Endophyte Composition In Zea Mays, Breena Lori Nolan

Honors Theses

The community structure of the endophytic bacteria in Zea mays roots was examined for the potential effects of glyphosate application, tillage strategies, and whether or not the corn plant in question was of an organic or glyphosate-resistant variety. Roots were harvested from plots designated to receive their specific treatments at the USDA-ARS Crop Production Systems Research Unit Farm. Vortexing, sonication, and tissue grinding, extraction, and next generation sequencing of 16S rRNA genes from these roots were used to describe their bacterial community composition. Results indicated significant differences in the bacterial communities correlated to tillage practice or corn type, whereas glyphosate …

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The Effects Of Oral Probiotic Supplements On The Human Gut Microbiome, Erin Hudnall Jan 2016

The Effects Of Oral Probiotic Supplements On The Human Gut Microbiome, Erin Hudnall

Honors Theses

A diverse bacterial community makes up the human gut microbiome and is essential not only to digestion, but also to other physiological processes. This bacterial community is subject to change due to external factors including, but not limited to, age, diet, and lifestyle. This study observed the effects of oral probiotic supplements on this bacterial community. Fecal samples were collected from a single subject once each week during alternating two- to six-week cycles of taking and abstaining from the probiotic supplement. The samples were purified, the bacterial cells within lysed, and the enclosed DNA was collected. A portion of the …

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Homocysteine And Csrp-3 Protein In Migrating Neural Crest Cells: A Quantitative And Qualitative Confocal Microscopy Study, Allysan R. White Jan 2016

Homocysteine And Csrp-3 Protein In Migrating Neural Crest Cells: A Quantitative And Qualitative Confocal Microscopy Study, Allysan R. White

Dissertations and Theses @ UNI

Congenital heart defects are one of the leading causes of infant death. High maternal blood serum levels of the amino acid homocysteine and the frequency of heart defects are correlated, but the immediate cause is unknown. During early embryonic development, a distinctive population of cells arises dorsally as the neural tube closes, forming the brain and spinal cord. These are the neural crest cells, which play a crucial role in the development of the nervous system, head skeletal, certain endocrine and connective tissues, and, notably, the heart’s outflow region. These migratory cells will follow specific pathways and respond to signals …

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Methods Of Quantification And Characterization Of Coccidian Oocysts, Mariam Abunemeh Jan 2016

Methods Of Quantification And Characterization Of Coccidian Oocysts, Mariam Abunemeh

Honors Theses

Coccidiosis is a major economic and health risk in the poultry industry. The oocysts of the causative agent of coccidiosis are excreted in animal feces and must be ingested by a new host for a new infection to begin. These oocysts are microscopic and very similar between species. The ability to quantify and identify the oocysts that are causing the illness is important to controlling this disease. My research first compares methods of quantifying oocysts of domestic turkeys for their ease of use and accuracy. Next, I attempt to identifying novel oocysts from a different turkey species by morphological and …

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Optimizing Rna Extraction From Fungal-Algal Tissues For Transcriptomic Gene Expression Analysis, Michael J. Hohl Jan 2016

Optimizing Rna Extraction From Fungal-Algal Tissues For Transcriptomic Gene Expression Analysis, Michael J. Hohl

Honors Theses

Extracting high-quality RNA is critical for downstream applications such a qRT-PCR and RNA-Seq based transcriptomics. Single-stranded RNA is readily susceptible to degradation by environmental RNases, necessitating rapid and sterile homogenization techniques; freezing at -80°C, lysis buffer addition, and short bursts of beadbeating have been shown to increase yields and quality of extracted RNA. Utilizing fungal-algal tissues from the model symbiosis between the filamentous fungi Aspergillus nidulans and the green alga Chlamydomonas reinhardtii, three different commercial homogenizers and six RNA extractions kits were assessed and an optimized extraction protocol for total RNA from fungal-algal tissues was determined. Using the protocol designed …

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Evaluation Of Students' Satisfactions With And Importance Of Attributes Of Three Unique All-You-Can-Eat University Dining Facilities, Ruth Zegel Jan 2016

Evaluation Of Students' Satisfactions With And Importance Of Attributes Of Three Unique All-You-Can-Eat University Dining Facilities, Ruth Zegel

Honors Theses

This study was conducted to evaluate students' satisfaction with and importance of nine attributes of three unique all-you-can-eat university dining facilities at the University of Mississippi, the Grill at 1810, Marketplace at the Residential College, and the Rebel Market. The attributes included food quality, service quality, variety of menu items, cleanliness, nutritional content, availability of healthy options, location, atmosphere/environment, and image/reputation. Additionally, the study evaluated students' satisfaction with each university dining facility overall, the students' satisfaction versus importance at each facility. This information was used to determine attributes that may need improving within each university dining facility and areas that …

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