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Life Sciences

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Utah State University

1993

Human

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Rabbit And Human Non-Keratinising Stratified Squamous Oesophageal Epithelium Displays Similar Microridge Structure By Scanning Electron Microscopy, S. Shasha'a, G. R. Dickson, R. St. C. Gilmore, G. C. Crean, M. M. Butt, K. E. Carr Sep 1993

Rabbit And Human Non-Keratinising Stratified Squamous Oesophageal Epithelium Displays Similar Microridge Structure By Scanning Electron Microscopy, S. Shasha'a, G. R. Dickson, R. St. C. Gilmore, G. C. Crean, M. M. Butt, K. E. Carr

Scanning Microscopy

Since the oesophageal epithelium of common laboratory animals, rats and mice, is keratinized it is unsuitable for comparison with typical non-keratinized stratified squamous human epithelium. It is thus important to find a suitable animal model for the study of human oesophageal tissue changes. This study investigated the microridge structure of immature and adult rabbit specimens, and adult human biopsies by scanning electron microscopy and morphometry. The investigation revealed a similarity between typical squamous human and adult rabbit oesophageal mucosal epithelium. While human epithelium specimens subdivided into two other groups (non-typical squamous and non-squamous); all typical squamous human biopsies were from …


Comparative Aspects Of Splenic Microcirculatory Pathways In Mammals: The Region Bordering The White Pulp, E. E. Schmidt, I. C. Macdonald, A. C. Groom May 1993

Comparative Aspects Of Splenic Microcirculatory Pathways In Mammals: The Region Bordering The White Pulp, E. E. Schmidt, I. C. Macdonald, A. C. Groom

Scanning Microscopy

Splenic microcorrosion casts prepared using minimal volumes of material show that most of the flow passes through the region bordering the white pulp. However, the nature of these microcirculatory pathways has received little attention. We have studied these in dog, cat, rat, mouse, and normal versus diseased human spleens. In all 5 species, a marginal sinus (MS) of anastomosing vascular spaces 5-10 μm thick lies between the white pulp and marginal zone (MZ). The morphology differs between species and the MS is absent in immune thrombocytopenia. The MS fills by circumferential flow before blood passes outward to the MZ. Many …


Scanning Electron Microscopy Of Styrene-Methylethylketone Casts Of The Airway And The Arterial System Of The Lung, Teruyuki Hojo Jan 1993

Scanning Electron Microscopy Of Styrene-Methylethylketone Casts Of The Airway And The Arterial System Of The Lung, Teruyuki Hojo

Scanning Microscopy

The method of making casts of airways and the pulmonary arterial system using a styrene polymer resin is described. A human and a dog were used as models. The viscosity of this resin ranges between 40 and 60 dPa/s at 24°C. The elastic rubber tube used for resin injection allows a constant perfusion pressure, i.e., 44 mm Hg and 110 mm Hg for airway and vascular filling, respectively. The casts obtained using this method are suitable both for macro- and microscopic analysis. Shrinkage during polymerization is minimal. Moreover, a preceding lavage is not necessary. Scanning electron microscopy (SEM) of cast …