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Methods For Characterization Of Cl-001 Derived Exosomes As A Stimulating Agent For Nk Cells For Immunotherapy, Ilana Gomez Diaz
Methods For Characterization Of Cl-001 Derived Exosomes As A Stimulating Agent For Nk Cells For Immunotherapy, Ilana Gomez Diaz
Honors Undergraduate Theses
The immune system is a complex set of cells in the body that work to prevent disease or infection by recognizing and eliminating foreign material or unhealthy cells. One essential type of immune cell that is part of the innate immune response are Natural Killer (NK) cells. These cytotoxic effector lymphocytes can detect certain unhealthy cells, such as cancer cells, that normal B and T cells would miss. For example, many tumor cells have evolved to bypass immune system surveillance by not expressing major histocompatibility complex class I molecules (MHC class I), on their surface, reducing recognition by cytotoxic T …
Investigation Of Catalysis Of Nitration By Cytochrome P450s, Lannika Johnson
Investigation Of Catalysis Of Nitration By Cytochrome P450s, Lannika Johnson
Honors Undergraduate Theses
TxtE is a protein related to cytochrome P450 enzymes, which catalyze a number of reactions that typically involve oxygen and not nitrogen. It has been discovered that TxtE can nitrate tryptophan through an unusual reaction in which it uses nitric oxide (NO) as a nitrogen donor to install the nitro group despite NO typically being considered toxic to bacteria. This project will determine if all cytochromes P450 can catalyze nitration as long as they are given NO. This will have an impact on understanding drug delivery and metabolism for which nitration is important.
Exploration Of Warm-Up Protocols On Muscular Fatigue, Sahil Kapadia
Exploration Of Warm-Up Protocols On Muscular Fatigue, Sahil Kapadia
Honors Undergraduate Theses
Muscular dystrophy is a genetically linked myopathy with no cure available. The lack of a cure makes early detection and treatment of muscular dystrophy imperative. When reviewing protocols examining muscular fatigue at submaximal isometric contractions, proper warm-up appeared to be absent and could have caused skewed results and conclusions. This study examines the effects of implementing a warm-up protocol before fatiguing trials. In this study, 10 adult subjects conducted fatiguing protocols with the right rectus at submaximal isometric contractions. The warm-up period included a light walk along with contractions at 20% and 33% of maximal voluntary isometric contraction (MVIC) levels. …
Microplastic Abundances In The Guana River Estuary In Northeast Florida, Mckenna Keplinger
Microplastic Abundances In The Guana River Estuary In Northeast Florida, Mckenna Keplinger
Honors Undergraduate Theses
Plastic never fully disappears, but instead breaks into smaller pieces referred to as microplastics (< 5 mm length). Microplastics are common worldwide, and more studies are needed to understand the accumulation and diversity of microplastics found in various environments. In this study, six locations were sampled for one year in the Guana River Estuary, a partially impounded system with heavily urbanized headwaters. This study was conducted in conjunction with the Guana Tolomato Matanzas National Estuarine Research Reserve (GTM NERR) and the Florida Fish and Wildlife Conservation Commission (FWC) at their water sampling stations. The objectives of this study were to investigate: 1) distribution patterns across sample sites over time, 2) the color, size, and shape of microplastics found, and 3) polymer composition. Water samples were collected from surface waters in 1-L bottles, with five replicates at each site. Sampling occurred once a month, at the beginning of each month from August 2020-August 2021. Samples were analyzed for abundance and characteristics of microplastics using a dissecting microscope and the polymer composition was determined using Fourier-transform infrared spectroscopy. No pattern was found in the distribution of microplastics from north to south or over time. Variations in plastic color, size, and polymer composition suggests that there are multiple sources of pollution into the Guana River Estuary.
Comparative Evaluation Of Assemblers For Metagenomic Data Analysis, Matheus Pavini Franco Ferreira
Comparative Evaluation Of Assemblers For Metagenomic Data Analysis, Matheus Pavini Franco Ferreira
Honors Undergraduate Theses
Metagenomics is a cultivation-independent approach for obtaining the genomic composition of microbial communities. Microbial communities are ubiquitous in nature. Microbes which are associated with the human body play important roles in human health and disease. These roles span from protecting us against infections from other bacteria, to being the causes of these diseases. A deeper understanding of these communities and how they function inside our bodies allows for advancements in treatments and preventions for these diseases. Recent developments in metagenomics have been driven by the emergence of Next-Generation Sequencing technologies and Third-Generation Sequencing technologies that have enabled cost-effective DNA sequencing …
Flashbulb Memories Among College Students During Covid-19, Sabah Qureshi
Flashbulb Memories Among College Students During Covid-19, Sabah Qureshi
Honors Undergraduate Theses
Flashbulb memories are formed through widely shared events that have affected the culture and community. The “flash” in flashbulb memories refers to the specific details that individuals have developed in their memories. The presented research focuses on the specific event of college students at the University of Central Florida (UCF) hearing about university closure due to the COVID-19 pandemic. This research study sought to identify the variables that have affected the accuracy of flashbulb memories formation regarding the event of college students hearing about university closure because of the pandemic. The variables of the students’ relevance to the university, location, …
The Use Of Exogenous Acrp30 As A Promising Amelioratory Compound Regarding Insulin Resistance And Type Ii Diabetes Mellitus, Stephen L. Romero
The Use Of Exogenous Acrp30 As A Promising Amelioratory Compound Regarding Insulin Resistance And Type Ii Diabetes Mellitus, Stephen L. Romero
Honors Undergraduate Theses
As numerous bodies of research have characterized the adipocytokine adiponectin (ACRP30) as an anti-diabetic compound, this work serves as an analysis to partly elucidate the potential usage of supplementary adiponectin as a therapeutic compound in the treatment of type II diabetes mellitus. In this work, a meta-analysis was conducted to centralize large amounts of data on adiponectin’s insulin sensitizing characteristics in conjunction with like-minded studies utilizing metformin treatment as the diabetic standard of care. Upon comparison of fasting glucose, fasting insulin, and glucose tolerance test area under the curve reduction percentages, it is quite clear that ACRP30 has significant insulin …
A Systematic Review And Meta-Analysis Of The Relationship Between The Creb Protein's Neuroplastic Functions And The Implications In Neurodegenerative Diseases: A Possible Link Between Synaptic Plasticity And Neurodegenerative Diseases, Mani Sarmast
Honors Undergraduate Theses
In this two-part study, I investigated whether the cyclic-adenosine monophosphate response element-binding (CREB) protein has the potential to be clinically modulated as a therapeutic target for the treatment of neurodegenerative diseases. Part one consisted of a systematic review that was conducted on select articles gathered through a stepwise method to explore (1) the relationship between diseased, neurodegenerative brains and levels of active, phosphorylated CREB (pCREB), (2) increased activation of CREB as a treatment for neurodegenerative symptoms, and (3) a potential therapeutic drug for neurodegenerative diseases that can target CREB signaling. The results of the systematic review showed evidence that suggested …
The Relationship Between Inflammatory Diet Score And Cancer Outcomes: Systematic Review And Meta-Analysis, Eric Han
Honors Undergraduate Theses
Cancer remains one of the most prevalent diseases in the United States and a leading cause of death. Large prospective studies have found significant correlations between dietary intake and cancer. Chronic inflammation promotes pro-cancer inflammatory environments promoting the formation and growth of tumors while preventing effective anti-tumor responses. Nutrition can impact inflammation, with the intake of certain food items increasing biomarkers for systemic inflammation thus, the objective of this research was to explore the relationship between inflammatory diet score measured by the Dietary Inflammatory index and all-cause mortality, cancer-specific mortality, and cancer recurrence among cancer survivors. Web of Science, Medline, …
Genomic Analysis Of Pollen Grains For Forensic Applications, Luz Kelley
Genomic Analysis Of Pollen Grains For Forensic Applications, Luz Kelley
Electronic Theses and Dissertations, 2020-
With over 300,000 plant species on the planet and more than 90% of them relying on pollen for reproduction, palynology, the study of pollen grains plays a vital role in many research fields. One of them is forensic palynology, which uses pollen as a proxy to link individuals or objects to a location or instance. It relies on the fact that (1) pollen is an ever-present feature of the environment; (2) different locations have different pollen signatures, allowing for inference related to spatial tracking; (3) plants bloom at different times, allowing for temporal inference; and (4) pollen is exceptionally durable …
Juvenile Dispersal And Genetic Connectivity In The Sea Turtle 'Lost Years', Katrina Phillips
Juvenile Dispersal And Genetic Connectivity In The Sea Turtle 'Lost Years', Katrina Phillips
Electronic Theses and Dissertations, 2020-
Juvenile dispersal is a life history strategy found across taxa and habitat types. In this dissertation, I examine juvenile dispersal in an evolutionary context and explore the conditions that support high dispersal, which necessitates subsequent ontogenetic habitat shifts. I use a sea turtle study system to evaluate the genetic and behavioral patterns associated with juvenile dispersal and ontogenetic shifts in marine environments. Specifically, I focus on four sea turtle species found in the northeastern Gulf of Mexico in the early life stage known as the 'lost years': green turtles (Chelonia mydas), Kemp's ridleys (Lepidochelys kempii), loggerheads (Caretta caretta), and hawksbills …
Complex Dna Mixture Deconvolution By Single Cell Genomics: Direct Physical Cell Subsampling And High Sensitivity Dna Typing, Kaitlin Huffman
Complex Dna Mixture Deconvolution By Single Cell Genomics: Direct Physical Cell Subsampling And High Sensitivity Dna Typing, Kaitlin Huffman
Electronic Theses and Dissertations, 2020-
Biological evidence is often found at crime scenes and can comprise of DNA from the victim(s) and perpetrator(s) to a crime as well as from individuals with no direct relationship to the incident. This can complicate analysis as DNA mixtures are one of the more difficult sources of biological evidence to interpret. Probabilistic genotyping (PG) has greatly aided in mixture analysis. However, even with PG, standard bulk mixture approaches do not always result in probative results as allele overlap, artifacts, or low-level minor contributors inevitably cause genotype information loss. Therefore, deconvolution of forensic DNA mixtures into their individual component DNA …
Intrahost Diversity Of Staphylococcus Aureus In Carriage Among Native Americans, Julia Webb
Intrahost Diversity Of Staphylococcus Aureus In Carriage Among Native Americans, Julia Webb
Electronic Theses and Dissertations, 2020-
Staphylococcus aureus (SA) is an opportunistic pathogen and a commensal member of the human microbiota that frequently colonizes the upper respiratory tract and skin. While SA can cause disease ranging from minor skin infections to life-threatening septicemia, it can also be carried asymptomatically. As carriage is the most significant risk factor for SA disease, surveillance is important for the prevention of outbreaks in vulnerable communities. Native Americans have a greater risk of infectious disease than the general US population, and Native Americans in the Southwestern United States have been shown to experience high rates of invasive SA disease. Here we …
Fibropapillomatosis And Chelonid Herpesvirus 5 Dynamics In Juvenile Green Turtles Of The Indian River Lagoon, Florida, Usa, Jake Kelley
Electronic Theses and Dissertations, 2020-
Fibropapillomatosis (FP) is a tumor-forming sea turtle disease that mainly affects juvenile green turtles (Chelonia mydas) in coastal foraging sites. The cause of FP is unclear, but likely involves the putative pathogen chelonid herpesvirus 5 (ChHV5). The overall dynamics of this disease are poorly understood, in part due to the difficulty of inferring disease and infection statuses of turtles without evidence of FP. In this study, I investigated the FP disease system in juvenile green turtles of the Indian River Lagoon (IRL), Florida, USA, to better understand disease and pathogen dynamics. First, I developed a hierarchical model for predicting FP …
The Test-Retest Reliability Of Single Leg Jump Performance Using The Drift Protocol In Division I Baseball Pitchers, Amy Bergquist
The Test-Retest Reliability Of Single Leg Jump Performance Using The Drift Protocol In Division I Baseball Pitchers, Amy Bergquist
Electronic Theses and Dissertations, 2020-
The purpose of this study was to determine the test-retest reliability of the Drift protocol with and without utilizing an arm swing, and to establish if this is an effective assessment tool for single leg vertical jump. Thirteen male Division I Baseball pitchers (18-35 yrs.) completed four testing visits where a single leg hopping protocol was assessed. Visit one consisted of the consent process and familiarization with the Drift protocol. Visits two, three, and four involved two trials of the protocol, with and without an arm swing. The protocol consisted of five single leg hops on both the stride and …
Investigating The Relationship Between Biological Age And Mutant Huntingtin Misfolding, Ratnesh Kesineni
Investigating The Relationship Between Biological Age And Mutant Huntingtin Misfolding, Ratnesh Kesineni
Honors Undergraduate Theses
Huntington Disease (HD) is an autosomal dominant neurodegenerative disease caused by an expansion of a CAG trinucleotide repeat tract in the huntingtin (HTT) gene. This CAG tract expansion causes production of mutant HTT (mtHTT) protein, which misfolds and forms inclusions in the brain that accumulate with age. Misfolded mtHTT aggregates have been linked with increased cell death in neuron cell culture, leading to speculation that mtHTT aggregates cause cell death. However, there are mouse lines that have robust mtHTT inclusion deposition, but no HD-like signs or neurodegeneration. Furthermore, neuronal HD cultured cells with inclusions were found to be …
The Effect Of Young Blood Anti-Aging Treatment On Protein Markers Of Age And Huntington Disease, Sujena H. Koilpillai
The Effect Of Young Blood Anti-Aging Treatment On Protein Markers Of Age And Huntington Disease, Sujena H. Koilpillai
Honors Undergraduate Theses
Huntington disease (HD) is a neurodegenerative disorder caused by expansion of a polyglutamine tract within the huntingtin (HTT) protein, forming mutant HTT (mtHTT). HD patients suffer from psychiatric, behavioral, cognitive, and motor abnormalities, with death typically occurring 15-20 years after symptom onset. Currently, there are no treatments able to slow disease progression or delay onset. HD is a disease of aging. Despite the mtHTT protein being produced throughout life, symptoms do not typically appear until adulthood. Furthermore, many cellular effects of normal aging are also seen in HD, including altered intercellular communication and loss of proteostasis. Recently, our lab found …
Novel Microbe-Resistant Clay Dressing For Healing Burn Wounds, Kasey L. Rigby
Novel Microbe-Resistant Clay Dressing For Healing Burn Wounds, Kasey L. Rigby
Honors Undergraduate Theses
Every year, about 550,000 patients receive medical attention for minor and major burns in the United States.1 In 2020, it was estimated that 11 million people worldwide suffered from burn injuries, with 150,000 of those burns being fatal.2 Burns are among the most painful and debilitating recalcitrant wounds that can often turn terminal when infection occurs. The different grades for burns that we aim to treat are first, second, and third degrees.2 Each burn type is susceptible to secondary infection that can be life threatening, and as a result, are extensively treated with antimicrobial agents.2 At present, …
The Role Of The Prefrontal Cortex And Stress In Huntington Disease-Mediated Aggression, Kadambari Vyas
The Role Of The Prefrontal Cortex And Stress In Huntington Disease-Mediated Aggression, Kadambari Vyas
Honors Undergraduate Theses
Huntington Disease (HD) is a fatal neurodegenerative disorder that is characterized by motor, cognitive, and psychiatric symptoms. Although HD onset is determined by motor symptoms, psychiatric symptoms, like depression and aggression, can develop earlier, have a larger impact on quality of life, and are understudied due to stigma. Our lab has observed hyper aggression in our humanized HD mouse model (Hu97/18) compared to our knock-in HD mouse model (Q175FDN). We characterized these differences and found that the Hu97/18 mice overreact in neutral situations, behaving as if they are in threatening situations. We are now using this novel model of HD-related …
Computational Methods To Analyze Next-Generation Sequencing Data In Genomics And Metagenomics, Saidi Wang
Computational Methods To Analyze Next-Generation Sequencing Data In Genomics And Metagenomics, Saidi Wang
Electronic Theses and Dissertations, 2020-
This thesis focuses on two important computational problems in genomics and metagenomics with the public available next-generation sequencing data. One is about gene regulation, for which we explore how distal regulatory elements may interact with the proximal regulatory elements. The other is about metagenomics, in which we study how to reconstruct bacterial strain genomes from shotgun reads. Studying gene regulation, especially distal gene regulation, is important because regulatory elements, including those in distal regulatory regions, orchestrate when, where and how much a gene is activated under every experimental condition. Their dysfunction results in various types of diseases. Moreover, the current …
Multiomic Hypotheses Underlying Behavioral Manipulation Of Camponotus Floridanus Ants By Ophiocordyceps Camponoti-Floridani Fungi, Ian Will
Electronic Theses and Dissertations, 2020-
Parasitic manipulation of host behavior lies at the intersection of disease, animal behavior, and coevolutionary processes. In many of these interactions, the underpinning biology is brought into sharp focus as they are obligate relationships, under strong selection to bring about specific changes in host behavior that determine if the parasite will transmit or die. However, experimental and molecular techniques to understand these interactions are still developing and identification of the mechanisms of manipulation is a primary goal in the field. As such, we investigated host-parasite interactions between Camponotus floridanus (Florida carpenter ant) and Ophiocordyceps camponoti-floridani (Florida zombie ant fungus) from …
Automatic Posture Correction Utilizing Electrical Muscle Stimulation, Ravi Kiran Kattoju
Automatic Posture Correction Utilizing Electrical Muscle Stimulation, Ravi Kiran Kattoju
Electronic Theses and Dissertations, 2020-
Habitually poor posture can lead to repetitive strain injuries that lower an individual's quality of life and productivity. Slouching over computer screens and smart phones, asymmetric weight distribution due to uneven leg loading, and improper loading posture are some of the common examples that lead to postural problems and health ramifications. To help cultivate good postural habits, researchers have proposed slouching, balance, and improper loading posture detection systems that alert users through traditional visual, auditory or vibro-tactile feedbacks when posture requires attention. However, such notifications are disruptive and can be easily ignored. We address these issues with a new physiological …
"Reeling In" Juvenile Sportfish Through Coastal Habitat Restoration: Population, Community, And Trophic Responses In The Indian River Lagoon, Florida, Jennifer Loch
Electronic Theses and Dissertations, 2020-
Coastal habitats provide crucial nursery habitat for predatory fishes, but they are in decline worldwide, impacting economically important fisheries. Habitat restoration can simultaneously mitigate the effects of habitat loss and benefit predators (e.g., sportfish), although this relationship is understudied. Here, the response of juvenile sportfish to oyster reef and living shoreline restoration is compared to controls in the Indian River Lagoon, Florida prior to and following restoration for up to three years through examination of community (diversity, assemblage), population (abundance), biometric (size, body condition), ontogenetic, and trophic (gut contents, stable isotopes) dynamics. Stable isotopes were used to quantify dietary history, …
Mathematical Model For Giraffe Population Dynamics, Huntir Bass
Mathematical Model For Giraffe Population Dynamics, Huntir Bass
Electronic Theses and Dissertations, 2020-
Since the 1980s the overall giraffe population has dropped at least 40% causing some researchers to label this rapid decline as the "Silent Extinction." Due to this plummet, understanding the behaviors of the giraffe population is absolutely necessary before they are on the brink of extinction. Through the usage of mathematical modeling methodologies, a general model is created to illustrate the relationship between juvenile and adult female giraffes through numerous interaction parameters. Variations on specific variables generate different simulations, which allows more biological accuracy. With each variation having an established coexistence equilibrium between the juvenile and adult female populations, the …
Sex Based Differences In Muscle Quality Recovery Following One Week Of Lower Limb Immobilization And Subsequent Retraining, Ryan Girts
Electronic Theses and Dissertations, 2020-
The present project represents the second part of a two-phase clinical trial designed to comprehensively examine the effects of knee joint immobilization and recovery on skeletal muscle size, strength, and central nervous system plasticity in healthy males and females. The purpose of this study was to examine differences between sexes in the recovery of muscle quality, size, and strength in response to a resistance training-based rehabilitation program following one week of knee joint immobilization. Twenty-seven participants (males: n = 16, age = 22 ± 3 years, BM = 81.3 ± 14.8 kg, BMI = 25.0 ± 3.4 kg/cm2; females: n …
From Dna Logic Gates To Dna Nanorobots, Tatiana Molden
From Dna Logic Gates To Dna Nanorobots, Tatiana Molden
Electronic Theses and Dissertations, 2020-
Due to their biocompatibility and parallel data processing, DNA computational devices are highly desired for applications in diagnosis and treatment of cancer, infectious and genetic diseases. Much like in modern electronic devices, DNA computation is based on the logic gates - by directly interacting with DNA or RNA input molecules, they produce a specific output depending on their embedded logic function. This work is devoted to the development of functional parts of a future DNA nanorobot for biomedical applications. Specifically, we used DNA nanotechnology and the concept of multicomponent DNA probes to develop three parts of the DNA nanorobot: computing, …
Characterization Of A Malachite Green Dna Aptamer And Development Of Malachite Green Dna Aptamer Sensor, Martin O'Steen
Characterization Of A Malachite Green Dna Aptamer And Development Of Malachite Green Dna Aptamer Sensor, Martin O'Steen
Electronic Theses and Dissertations, 2020-
Hydrogels have been extensively studied for use in applications such as tissue engineering, drug delivery systems, wound dressing, actuators, valves, and sensors in Micromechanical Systems (MEMS), among others. Thermo-responsive hydrogels in particular pose various advantages such as their capability to respond to an external stimulus and reverse the response when the stimulus is removed and the ability to imbibe a significant amount of solvent and increase their volume by over 1000%. Extensive research has been conducted to tune and improve hydrogel's rate and degree of swelling and mechanical properties. Previous work showed that when synthetic clay (Laponite) is cross-linked with …
A Look Into Alzheimer’S Disease—Interventions At The Molecular Level, Priscila Feliciano Nieves
A Look Into Alzheimer’S Disease—Interventions At The Molecular Level, Priscila Feliciano Nieves
Honors Undergraduate Theses
There are puzzle pieces to the cure for Alzheimer’s Disease (AD), and such can emerge by inspecting the biomolecular interactions and their effects on neuronal cells. The upcoming presented literature review will cover the molecular changes caused by AD pathological progression, explore the relationship between non-AD molecules and AD molecules in the body, and analyze potential contributing factors in AD. In addition, the information to be provided will highlight medicinal alternatives respective to a particular stage in AD.
Investigating The Phytochemical Profiles Of Wild And Cultivated Blueberries (Vaccinium Spp.) In Central Florida, Anisa Khalid
Investigating The Phytochemical Profiles Of Wild And Cultivated Blueberries (Vaccinium Spp.) In Central Florida, Anisa Khalid
Honors Undergraduate Theses
Blueberries (Vaccinium spp.) are valued for their high nutritional quality and flavor. There are five species in the Vaccinium genus native to the state of Florida, but only three are palatable to humans: V. myrsinites, V. darrowii, and V. corymbosum. While V. darrowii has been studied extensively due to its successful hybridization with northern highbush blueberry cultivars, V. myrsinites and wild V. corymbosum have been understudied. This study aims to 1) quantify the abundance of various phytochemicals in fruits of these wild blueberry species and compare fruit quality both among the wild taxa and two varieties of …
Effects Of Post-Exercise Recovery Drink Composition On Subsequent Performance In Masters Class Athletes, Erica Goldstein
Effects Of Post-Exercise Recovery Drink Composition On Subsequent Performance In Masters Class Athletes, Erica Goldstein
Electronic Theses and Dissertations, 2020-
Carbohydrate (CHO) and carbohydrate-protein coingestion (CHO-P) have been shown to be equally effective for enhancing glycogen resynthesis and subsequent same-day performance when CHO intake is suboptimal (≤0.8 g/kg). Few studies have specifically examined the effect of isocaloric CHO vs CHO-P consumption on subsequent high-intensity aerobic performance with limited time to recover (≤2 hours) in masters class endurance athletes. Participants (n = 22) were assigned to consume one of three beverages during a 2-hour recovery period: PLA (electrolytes and water), CHO (1.2 g/kg bm), or CHO-P (0.8 g/kg bm CHO + 0.4 g/kg bm PRO). All beverages were standardized to one …