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Nest Success Of Mountain Plover Relative To Anthropogenic Edges In Eastern Colorad, Christian Mettenbrink, Victoria Dreitz, Fritz Knopf Jan 2006

Nest Success Of Mountain Plover Relative To Anthropogenic Edges In Eastern Colorad, Christian Mettenbrink, Victoria Dreitz, Fritz Knopf

Victoria Dreitz

No abstract provided.


Issues In Species Recovery: An Example Based On The Wyoming Toad, Victoria Dreitz Jan 2006

Issues In Species Recovery: An Example Based On The Wyoming Toad, Victoria Dreitz

Victoria Dreitz

No abstract provided.


Amoeboid Form Of Blastocystis Hominis - A Detailed Ultrastructural Insight, Tan Tian Chye Jan 2006

Amoeboid Form Of Blastocystis Hominis - A Detailed Ultrastructural Insight, Tan Tian Chye

Tan Tian Chye

The amoeboid form of Blastocystis hominis has been reported infrequently, and its morphological descriptions have yielded conflicting and confusing reports. In the present study, we used the amoeboid forms seen predominantly in symptomatic patients infected with Blastocystis to provide detailed descriptions on the fine surface structure and intracellular morphology. Scanning electron microscopy revealed the irregular shape of the amoeboid form, with an intercalated fibrillar structure and a highly convoluted surface with deep indentations and projected pseudopodia. Transmission electron microscopy showed the existence of two types of amoeboid forms of B. hominis in in vitro culture, one with a large central …


Predominance Of Amoeboid Forms Of Blastocystis Hominis In Isolates From Symptomatic Patients, Tan Tian Chye Jan 2006

Predominance Of Amoeboid Forms Of Blastocystis Hominis In Isolates From Symptomatic Patients, Tan Tian Chye

Tan Tian Chye

Blastocystis hominis is one of the most common human parasites that inhabit the intestinal tract. Conflicting reports continue to exist regarding the existence and the functional role of the amoeboid forms in the life cycle of the parasite. The present study investigates the presence of these forms in 20 isolates obtained from ten symptomatic and asymptomatic patients respectively. A total of 10,000 parasite cells per ml from each isolate were inoculated into three culture tubes each containing 3 ml of Jones' medium supplemented with 10% horse serum, incubated at 37 degrees C. The contents were examined daily for 10 days. …


Pcr Fingerprinting Of Blastocystis Isolated From Symptomatic And Asymptomatic Human Hosts, Tan Tian Chye Jan 2006

Pcr Fingerprinting Of Blastocystis Isolated From Symptomatic And Asymptomatic Human Hosts, Tan Tian Chye

Tan Tian Chye

Genomic DNA from 16 Blastocystis hominis isolates comprising of eight asymptomatic isolates (A1-A8) and eight symptomatic isolates (S1-S8) was amplified by arbitrarily primed polymerase chain reaction (AP-PCR) using 38 arbitrary 10-mer primers. Six primers (A10, B5, C20, D1, F6, and F10) generated reproducible DNA fingerprints. AP-PCR amplification revealed similar DNA fingerprints among all symptomatic isolates (S1-S8) with common bands at 850 bp using primer A10, 920 bp using primer B5, and 1.3 kbp using primer D1. Isolates A1, A3, A4, A5, A6, and A7 showed similar DNA banding patterns and all asymptomatic isolates (A1-A8) shared a major band at I …


Effects Of Ascorbic Acid On Impaired Vascular Reactivity In Aortas Isolated From Age-Matched Hypertensive And Diabetic Rats, Mohd Rais Mustafa, Macha Ajay Jan 2006

Effects Of Ascorbic Acid On Impaired Vascular Reactivity In Aortas Isolated From Age-Matched Hypertensive And Diabetic Rats, Mohd Rais Mustafa, Macha Ajay

Mohd Rais Mustafa

Impaired vascular reactivity is a hallmark of several cardiovascular diseases that include hypertension and diabetes. This study compared the changes in vascular reactivity in age-matched experimental hypertension and diabetes, and, subsequently, tested whether these changes could be affected directly by ascorbic acid (10 microM). Endothelium-derived nitric oxide (NO) modulation of ascorbic acid effects was also investigated. All the experiments were performed in the presence of a cyclooxygenase inhibitor, indomethacin (10 microM). Results showed that the endothelium-dependent and -independent relaxations induced by acetylcholine (ACh) and sodium nitroprusside (SNP), respectively, were blunted to a similar extent in isolated aortic rings from age-matched …


Bioassay-Guided Isolation Of A Vasorelaxant Active Compound From Kaempferia Galanga L, Mohd Rais Mustafa, Khalijah Awang, Mustafa Ali Mohd, Rozana Othman, Halijah Ibrahim Jan 2006

Bioassay-Guided Isolation Of A Vasorelaxant Active Compound From Kaempferia Galanga L, Mohd Rais Mustafa, Khalijah Awang, Mustafa Ali Mohd, Rozana Othman, Halijah Ibrahim

Mohd Rais Mustafa

Bioassay-guided fractionation was performed on a crude dichloromethane extract of Kaempferia galanga L. using chromatography techniques. Screening of the extract for biological activity started with the brine shrimp lethality bioassay, followed by the study of its antihypertensive activity on anaesthetized rats, which involved monitoring of the extract's effect on mean arterial blood pressure. The components of the fractions obtained from the separation procedures were analyzed using gas chromatography (GC). The yield of the CH2Cl2 extract was 0.29% of the crude plant extract. Analysis of the data for brine shrimp lethality test using the Finney computer program showed that this extract …


Examining The Direction Of Imagery And Self-Talk On Dart-Throwing Performance And Self Efficacy, Jennifer Cumming, Sanna M. Nordin, Robin Horton, Scott Reynolds Jan 2006

Examining The Direction Of Imagery And Self-Talk On Dart-Throwing Performance And Self Efficacy, Jennifer Cumming, Sanna M. Nordin, Robin Horton, Scott Reynolds

Jennifer Cumming

The study investigated the impact of varying combinations of facilitative and debilitative imagery and self-talk (ST) on self-effi cacy and performance of a dart-throwing task. Participants (N = 95) were allocated to 1 of 5 groups: (a) facilitative imagery/facilitative ST, (b) facilitative imagery/debilitative ST, (c) debilitative imagery/facilitative ST, (d) debilitative imagery/debilitative ST, or (e) control. Mixed-design ANOVAs revealed that performance, but not self-effi - cacy, changed over time as a function of the assigned experimental condition. Participants in the debilitative imagery/debilitative ST condition worsened their performance, and participants in the facilitative imagery/facilitative ST condition achieved better scores. These fi ndings …


Phenotypic And Genetic Parameters Of Reproductive Traits For Ayrshire Cattle On Large-Scale Farms In Kenya, Joshua O. Amimo Jan 2006

Phenotypic And Genetic Parameters Of Reproductive Traits For Ayrshire Cattle On Large-Scale Farms In Kenya, Joshua O. Amimo

Joshua O Amimo

No abstract provided.


Comparative Efficiency Of Six Stable Fly Traps., David B. Taylor, Dennis R. Berkebile Jan 2006

Comparative Efficiency Of Six Stable Fly Traps., David B. Taylor, Dennis R. Berkebile

David B Taylor

Five adhesive traps and the Nzi cloth-target trap were compared to determine their trapping efficiency and biases for stable flies, Stomoxys calcitrans (L.) (Diptera: Muscidae). Two confgurations of the BiteFree prototype trap, constructed of polyethylene terephthalate, were most efficient for trapping stable flies, whereas the EZ trap was least efficient. The two Alsynite traps, Broce and Olson, were intermediate to the BiteFree prototype and EZ traps. All adhesive traps collected a ratio of approximately two males for each female. Approximately 50% of the flies collected on the adhesive traps, both male and female, were blood fed, and 20% were vitellogenic. …


Use Of Fatty Acid Profiles To Distinguish Between Selected Game Fish And Farm-Raised Channel Catfish, Randy S. Stahl, Brian S. Dorr, Scott C. Barras, John J. Johnston Jan 2006

Use Of Fatty Acid Profiles To Distinguish Between Selected Game Fish And Farm-Raised Channel Catfish, Randy S. Stahl, Brian S. Dorr, Scott C. Barras, John J. Johnston

Brian S Dorr

No abstract provided.


On The Robustness Of Robustness Checks Of The Environmental Kuznets Curve, Marzio Galeotti, Matteo Manera, Alessandro Lanza Jan 2006

On The Robustness Of Robustness Checks Of The Environmental Kuznets Curve, Marzio Galeotti, Matteo Manera, Alessandro Lanza

Matteo Manera

Since its first inception in the debate on the relationship between environment and growth in 1992, the Environmental Kuznets Curve has been subject of continuous and intense scrutiny. The literature can be roughly divided in two historical phases. Initially, after the seminal contributions, additional work aimed to extend the investigation to new pollutants and to verify the existence of an inverted-U shape as well as assessing the value of the turning point. The following phase focused instead on the robustness of the empirical relationship, particularly with respect to the omission of relevant explanatory variables other than GDP, alternative datasets, functional …


Lesser Scaup Population Dynamics: What Can Belearned From Available Data?, David N. Koons, J. J. Rotella, D. W. Willey, M. Taper, R. G. Clark, S. Slattery, R. W. Brook, R. M. Corcoran, J. R. Lovvorn Jan 2006

Lesser Scaup Population Dynamics: What Can Belearned From Available Data?, David N. Koons, J. J. Rotella, D. W. Willey, M. Taper, R. G. Clark, S. Slattery, R. W. Brook, R. M. Corcoran, J. R. Lovvorn

David N. Koons

Populations of Lesser Scaup (Aythya affinis) have declined markedly in North America since the early 1980s. When considering alternatives for achieving population recovery, it would be useful to understand how the rate of population growth is functionally related to the underlying vital rates and which vital rates affect population growth rate the most if changed (which need not be those that influenced historical population declines). To establish a more quantitative basis for learning about life history and population dynamics of Lesser Scaup, we summarized published and unpublished estimates of vital rates recorded between 1934 and 2005, and developed matrix life-cycle …


From Faculty For Undergraduate Neuroscience: Encouraging Innovation In Undergraduate Neuroscience Education By Supporting Student Research And Faculty Development, Eric Wiertelak, J. C. Hardwick, M. Kerchner, B. Lom, J. J. Ramirez Jan 2006

From Faculty For Undergraduate Neuroscience: Encouraging Innovation In Undergraduate Neuroscience Education By Supporting Student Research And Faculty Development, Eric Wiertelak, J. C. Hardwick, M. Kerchner, B. Lom, J. J. Ramirez

Eric Wiertelak

No abstract provided.


Survival And Growth Of Two Heterotrophic Hydrothermal Vent Archaea, Pyrococcus Strain Gb-D And Thermococcus Fumicolans, Under Low Ph And High Sulfide Concentrations In Combination With High Temperature And Pressure Regimes, Virginia P. Edgcomb, Stephen J. Molyneaux, Simone Böer, Carl O. Wirsen, Mark Saito, Michael S. Atkins, Karen Lloyd, Andreas Teske Jan 2006

Survival And Growth Of Two Heterotrophic Hydrothermal Vent Archaea, Pyrococcus Strain Gb-D And Thermococcus Fumicolans, Under Low Ph And High Sulfide Concentrations In Combination With High Temperature And Pressure Regimes, Virginia P. Edgcomb, Stephen J. Molyneaux, Simone Böer, Carl O. Wirsen, Mark Saito, Michael S. Atkins, Karen Lloyd, Andreas Teske

Karen Lloyd

Growth and survival of hyperthermophilic archaea in their extreme hydrothermal vent and subsurface environments are controlled by chemical and physical key parameters. This study examined the effects of elevated sulfide concentrations, temperature, and acidic pH on growth and survival of two hydrothermal vent archaea (Pyrococcus strain GB-D and Thermococcus fumicolans) under high temperature and pressure regimes. These two strains are members of the Thermococcales, a family of hyperthermophilic, heterotrophic, sulfur-reducing archaea that occur in high densities at vent sites. As actively growing cells, these two strains tolerated regimes of pH, pressure, and temperature that were in most cases not tolerated …


Heterotrophic Archaea Dominate Sedimentary Subsurface Ecosystems Off Peru, Jennifer F. Biddle, Julius S. Lipp, Karen Lloyd, Ketil B. Sørensen, Rika Anderson, Helen F. Fredericks, Marcus Elvert, Timothy J. Kelly, Daniel P. Schrag, Mitchell L. Sogin, Jean E. Brenchley, Andreas Teske, Christopher H. House, Kaw-Uwe Hinrichs Jan 2006

Heterotrophic Archaea Dominate Sedimentary Subsurface Ecosystems Off Peru, Jennifer F. Biddle, Julius S. Lipp, Karen Lloyd, Ketil B. Sørensen, Rika Anderson, Helen F. Fredericks, Marcus Elvert, Timothy J. Kelly, Daniel P. Schrag, Mitchell L. Sogin, Jean E. Brenchley, Andreas Teske, Christopher H. House, Kaw-Uwe Hinrichs

Karen Lloyd

Studies of deeply buried, sedimentary microbial communities and associated biogeochemical processes during Ocean Drilling Program Leg 201 showed elevated prokaryotic cell numbers in sediment layers where methane is consumed anaerobically at the expense of sulfate. Here, we show that extractable archaeal rRNA, selecting only for active community members in these ecosystems, is dominated by sequences of uncultivated Archaea affiliated with the Marine Benthic Group B and the Miscellaneous Crenarchaeotal Group, whereas known methanotrophic Archaea are not detectable. Carbon flow reconstructions based on stable isotopic compositions of whole archaeal cells, intact archaeal membrane lipids, and other sedimentary carbon pools indicate that …


Ethylene Stimulates Nutations That Are Dependent On The Etr1 Receptor, Brad M. Binder, Ronan C. O'Malley, Wuyi Wang, Tobias C. Zutz, Anthony B. Bleeker Jan 2006

Ethylene Stimulates Nutations That Are Dependent On The Etr1 Receptor, Brad M. Binder, Ronan C. O'Malley, Wuyi Wang, Tobias C. Zutz, Anthony B. Bleeker

Brad M. Binder

Ethylene influences a number of processes in Arabidopsis (Arabidopsis thaliana) through the action of five receptors. In this study, we used high-resolution, time-lapse imaging to examine the long-term effects of ethylene on growing, etiolated Arabidopsis seedlings. These measurements revealed that ethylene stimulates nutations of the hypocotyls with an average delay in onset of over 6 h. The nutation response was constitutive in ctr1-2 mutants maintained in air, whereas ein2-1 mutants failed to nutate when treated with ethylene. Ethylene-stimulated nutations were also eliminated in etr1-7 loss-of-function mutants. Transformation of the etr1-7 mutant with a wild-type genomic ETR1 transgene rescued the nutation …


Soluble Fibrin Inhibits Monocyte Adherence And Cytotoxicity Against Tumor Cells: Implications For Cancer Metastasis, John Biggerstaff, Brandy Weidow, Jacqueline Vidosh, Judith Dexheimer, Shonak Patel, Pretesh Patel Jan 2006

Soluble Fibrin Inhibits Monocyte Adherence And Cytotoxicity Against Tumor Cells: Implications For Cancer Metastasis, John Biggerstaff, Brandy Weidow, Jacqueline Vidosh, Judith Dexheimer, Shonak Patel, Pretesh Patel

John Biggerstaff

Background Soluble fibrin (sFn) is a marker for disseminated intravascular coagulation and may have prognostic significance, especially in metastasis. However, a role for sFn in the etiology of metastatic cancer growth has not been extensively studied. We have reported that sFn cross-linked platelet binding to tumor cells via the major platelet fibrin receptor αIIbβ3, and tumor cell CD54 (ICAM-1), which is the receptor for two of the leukocyte β2 integrins (αLβ2 and aMβ2). We hypothesized that sFn may also affect leukocyte adherence, recognition, and killing of tumor cells. Furthermore, in a rat experimental metastasis model sFn pre-treatment of tumor cells …


The Importance Of Monkey Beetle (Scarabaeidae: Hopliini) Pollination For Aizoaceae And Asteraceae In Grazed And Ungrazed Areas At Paulshoek, Succulent Karoo, South Africa, Carolin Mayer, Geofrey Soka, Mike Picker Jan 2006

The Importance Of Monkey Beetle (Scarabaeidae: Hopliini) Pollination For Aizoaceae And Asteraceae In Grazed And Ungrazed Areas At Paulshoek, Succulent Karoo, South Africa, Carolin Mayer, Geofrey Soka, Mike Picker

Geofrey Soka

No abstract provided.


Farnesylated Lamins, Progeroid Syndromes And Farnesyl Transferase Inhibitors, Michael Sinensky, A. E. Rusinol Jan 2006

Farnesylated Lamins, Progeroid Syndromes And Farnesyl Transferase Inhibitors, Michael Sinensky, A. E. Rusinol

Michael Sinensky

Three mammalian nuclear lamin proteins, lamin B1, lamin B2 and the lamin A precursor, prelamin A, undergo canonical farnesylation and processing at CAAX motifs. In the case of prelamin A, there is an additional farnesylation-dependent endoproteolysis, which is defective in two congenital diseases: Hutchinson-Gilford progeria (HGPS) and restrictive dermopathy (RD). These two diseases arise respectively from defects in the prelamin A substrate and the enzyme (ZmpSte24) that processes it. Recent work has shed light on the roles of the lamin proteins and the enzymes involved in their farnesylation-dependent maturation. Other experimental work, including mouse model studies, have examined the possibility …


Dna Damage Responses In Progeroid Syndromes Arise From Defective Maturation Of Prelamin A, Michael Sinensky, Y. Liu, A. Rusinol, Y. Wang, Y. Zou Jan 2006

Dna Damage Responses In Progeroid Syndromes Arise From Defective Maturation Of Prelamin A, Michael Sinensky, Y. Liu, A. Rusinol, Y. Wang, Y. Zou

Michael Sinensky

The genetic diseases Hutchinson-Gilford progeria syndrome (HGPS) and restrictive dermopathy (RD) arise from accumulation of farnesylated prelamin A because of defects in the lamin A maturation pathway. Both of these diseases exhibit symptoms that can be viewed as accelerated aging. The mechanism by which accumulation of farnesylated prelamin A leads to these accelerated aging phenotypes is not understood. Here we present evidence that in HGPS and RD fibroblasts, DNA damage checkpoints are persistently activated because of the compromise in genomic integrity. Inactivation of checkpoint kinases Ataxia-telangiectasia-mutated (ATM) and ATR (ATM- and Rad3-related) in these patient cells can partially overcome their …