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Intricate Rna : Rna Interactions In U12-Dependent Nuclear Pre-Mrna Splicing, Tupa Basu Roy
Intricate Rna : Rna Interactions In U12-Dependent Nuclear Pre-Mrna Splicing, Tupa Basu Roy
ETD Archive
Coding regions or exons of most human genes are interrupted by noncoding intervening regions or introns. Removal of nuclear precursor messenger RNA (pre-mRNA) introns by RNA splicing is an essential step in eukaryotic gene expression. Two types of nuclear pre-mRNA introns are known as U2-dependent or major type and U12-dependent or minor type. Nuclear pre-mRNA introns are removed by two distinct sets of ribonucleoprotein complexes or spliceosomes, which are formed by five small nuclear RNAs (snRNAs) for each spliceosome. U6atac and U12 snRNAs are central to U12-dependent spliceosome and play essential roles in the removal of U12-dependent introns. U6atac and …
Targeting Expression Of An Oncogene By Splicing Interference (Splicei) In Human Mammary Carcinoma Cell Culture Model, Chaucola K. Pleasant
Targeting Expression Of An Oncogene By Splicing Interference (Splicei) In Human Mammary Carcinoma Cell Culture Model, Chaucola K. Pleasant
ETD Archive
In nuclear pre-mRNA splicing, introns are removed through cooperative interactions of small nuclear RNAs (snRNAs). Many human genes are interrupted by two types of nuclear pre-mRNA introns. Major class or the U2-dependent type introns are spliced by U1, U2, U4, U5 and U6 snRNAs. The minor class or U12-dependent type introns are spliced by U11, U12, U4atac, U5, and U6atac snRNAs. It has been shown that over expression of minor class spliceosomal snRNAs can have an inhibitory effect on the splicing of major class introns. To further test the concept, we targeted HER-2/Neu proto-oncogene, which is over-expressed in 20-30 of …
Characterization Of A Microrna Harboring Intron For Pre-Mrna Splicing And Microrna Processing, Neha Aggarwal
Characterization Of A Microrna Harboring Intron For Pre-Mrna Splicing And Microrna Processing, Neha Aggarwal
ETD Archive
Nuclear pre-mRNA splicing is an important step in eukaryotic RNA processing and can yield a variety of transcripts. Further, it is known that nuclear pre-mRNA introns are of two types, U2-dependent and U12-dependent. Recently discovered, microRNAs (miRNA) are short, endogenous, 20-22 nucleotide long, non-coding RNA molecules that base pair with target mRNAs to modulate translation. Current evidence suggests that processing of intronic miRNA does not affect the splicing. Since, intronic miRNAs are involved in essential cellular processes, we surmised if splice sites of miRNA coding introns are flexible to support productive precursor and mature miRNA processing. We constructed a minigene …