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Role Of Ribosomal Protein Us9/Ys16 In Translation Initiation And Elongation In Yeast Saccharomyces Cerevisiae., Supriya Jindal
Role Of Ribosomal Protein Us9/Ys16 In Translation Initiation And Elongation In Yeast Saccharomyces Cerevisiae., Supriya Jindal
ETD Archive
The process of translation in all living cells is performed by ribosomes and is divided into four major steps (initiation, elongation, termination and ribosome recycling). Ribosomes consist of ribosomal RNA (rRNA) and proteins and are composed of two subunits small and large. There are three major sites for tRNA binding within ribosome: the (aminoacyl) A-site which accepts the aminoacyl (aa)-tRNA; the P-site, where the peptidyl-tRNA is formed and the (exit) E-site, where deacylated tRNA exits the ribosome. These sites are formed by both rRNA and ribosomal proteins. Though rRNA are involved in the catalysis of protein synthesis, the contribution of …
Functional Diversity And Restoration Of Meadows In Northeast Ohio, Keri Plevniak
Functional Diversity And Restoration Of Meadows In Northeast Ohio, Keri Plevniak
ETD Archive
Restoration outcomes can be variable and there is a need to understand the short- and long-term responses of the vegetation community. It is important to see if management goals are being met by evaluating restoration outcomes. Restoration goals often include establishing native species, increasing conservation value, and providing pollinator resources throughout the year. Assessing communities is commonly done though a taxonomic approach by using species presence and abundance. Another method of evaluation is through functional traits or species traits and their abundance. Both give different perspectives on how the community is achieving management goals. While taxonomic assessments can give insight …
Insights Into The Ribosomal, Extra-Ribosomal And Developmental Role Of Rp L13a In Mammalian Model, Ravinder Kour
Insights Into The Ribosomal, Extra-Ribosomal And Developmental Role Of Rp L13a In Mammalian Model, Ravinder Kour
ETD Archive
Ribosomal protein L13a plays an extra-ribosomal function in translational silencing of GAIT (IFN-gamma-activated inhibitor of translation) element bearing mRNAs encoding inflammatory proteins but the underlying molecular mechanism of translational silencing and ribosomal incorporation of L13a remains poorly understood. Also, our laboratory showed that L13a acts as a physiological defense against uncontrolled inflammation in macrophage-specific knockout (KO) mice. However, the consequence of a total knockout of L13a in mammals remains unexplored. Therefore, our current study is focused on (i) identifying the amino acid residue(s) of L13a essential for incorporation and translational silencing of target mRNAs and (ii) studying the consequences of …
Investigation Of Ires-Mediated Translation Of Puma Mrna: Initiation Factor Requirements And Search For Itafs, Amra Ismail
Investigation Of Ires-Mediated Translation Of Puma Mrna: Initiation Factor Requirements And Search For Itafs, Amra Ismail
ETD Archive
Translation initiation is the rate-limiting and tightly regulated step of protein synthesis. Cap-dependent translation initiation accounts for about 95% of cellular mRNAs. Around 3-5% of cellular mRNAs have been found to contain a cis-regulatory element (IRES) which can recruit ribosomes in a cap-independent manner. IRESs support protein synthesis under cellular stress conditions when cap-dependent translation is inhibited. Differentiation in 23A2 myoblast cells can be induced by culturing cells in serum-free differentiating media (DM). During 23A2 cellular myoblast differentiation, approximately 30% of cells undergo apoptosis as a result of stress caused by serum withdrawal in order to induce differentiation. The expression …