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Revolutionary Advances In The Treatment Of Genetic Disease, Emma Kaitlyn Carrigan
Revolutionary Advances In The Treatment Of Genetic Disease, Emma Kaitlyn Carrigan
Honors Theses and Capstones
No abstract provided.
Investigation Of The Function Of Protein Acyl Transferases Using Crispr-Cas9 To Create Null Mutants, Claire E. Christopher
Investigation Of The Function Of Protein Acyl Transferases Using Crispr-Cas9 To Create Null Mutants, Claire E. Christopher
Honors Theses and Capstones
24 Protein Acyl Transferases (PATs) have been identified in the model organism Arabidopsis thaliana. Despite knowing the enzymatic function of these genes (palmitoylation), the specific subcellular pathways, protein targets, and cellular activities of these proteins remain unknown. To investigate the pathways that PAT genes are involved in, deletion mutations were created in several genes and the phenotype of the organisms carrying homozygous mutations was observed. To introduce mutations, the CRISPR/Cas9 system was inserted into the plant’s genome using Agrobacterium tumefaciens to cause deletions in PAT genes of interest. To date, no CRISPR-induced pat mutants have a noticeable phenotype. These …
Investigating The Role Of Bromodomain Proteins On Histone Post-Translational Modifications In Toxoplasma Gondii, Joshua Steven Berthiaume
Investigating The Role Of Bromodomain Proteins On Histone Post-Translational Modifications In Toxoplasma Gondii, Joshua Steven Berthiaume
Honors Theses and Capstones
Toxoplasma gondii is a prevalent pathogenic parasite that infects approximately 25 percent of the US population. For the parasite to successfully establish and maintain infection in its host, properly controlled regulation of gene expression is critical. One way Toxoplasma regulates gene expression is through modification of histone proteins that bind to DNA and can control gene accessibility. Acetylation is a modification that is added to histones that changes chromatin structure to enhance gene activation. Histone acetylation can also regulate gene expression by recruitment of important regulators such as bromodomain proteins (BDP). A previous study showed that loss of the bromodomain …