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Ecological Profiling Of Bird-Mosquito Interactions In Central Virginia, Anna Riggan Jul 2011

Ecological Profiling Of Bird-Mosquito Interactions In Central Virginia, Anna Riggan

Theses and Dissertations

Current methods of mosquito surveillance estimate general population abundances, but fail to represent the relationship of vector abundance to host density important to determining transmission risk of mosquito-borne pathogens (MBP). We sought to address this limitation by creating a novel mosquito trap that directly sampled mosquitoes seeking to feed on nesting birds. The primary objectives of this study were to (1) assess the efficiency of the Nest Mosquito Trap (NMT) and how this is affected by nest box size. (2) assess whether the NMT affects bird, specifically nest success in Prothonotary Warblers (Protonotaria citrea), and adult behavior. (3) compare our …


Growth Of Age-0 Atlantic Menhaden (Brevoortia Tyrannus) In Two Tidal Freshwater Tributaries Of Chesapeake Bay, Peter M. Sturke Jul 2011

Growth Of Age-0 Atlantic Menhaden (Brevoortia Tyrannus) In Two Tidal Freshwater Tributaries Of Chesapeake Bay, Peter M. Sturke

Theses and Dissertations

Few studies have described growth rates of age-0 Atlantic menhaden (Brevoortia tyrannus). Growth rates from tidal freshwater habitats of the Mattaponi and James Rivers, Virginia in 2009 were described and compared using otolith microstructural analyses. Larval tidal freshwater growth rates were significantly faster in the culturally eutrophic James River when compared to those collected from the Mattaponi River (p-value < 0.001). Elevated primary production within tidal freshwater habitats promotes favorable conditions for larval Atlantic menhaden growth. No differences between river habitats for juvenile growth rates were evident. Comparisons of age-0 growth rates to higher salinity habitats indicate that tidal freshwater habitats should be considered essential habitat for age-0 Atlantic menhaden.


Microbial Community Function In Freshwater Wetland Soils: Using Extracellular Enzyme Analysis To Study The Effect Of Moisture And Vegetation, Aaron Porter Jun 2011

Microbial Community Function In Freshwater Wetland Soils: Using Extracellular Enzyme Analysis To Study The Effect Of Moisture And Vegetation, Aaron Porter

Theses and Dissertations

Differences in microbial function via extracellular enzyme activity (EEA) were investigated to determine the potential effects of hydrology and plant-soil-microbe interactions in a young non-tidal freshwater riparian wetland. To study these relationships, three plots were established along a moisture gradient (Wet, Intermediate, Dry) within VCU Rice Center Within each main plot, five subplots were left undisturbed while another five were cleared of all above-ground plant biomass. Homogenized soil cores (top 10 cm) were analyzed for pH, redox, C:N, soil organic matter (SOM) content, saturation, and temperature. Microbial function was assessed using extracellular enzyme analysis. For most enzymes, a site difference …


The Superfund Program Past And Present Funding Implications, Nicole Franklin May 2011

The Superfund Program Past And Present Funding Implications, Nicole Franklin

Theses and Dissertations

The present research examines the impact of not reauthorizing the Superfund taxes on the operations of the program. EPA NPL site status data were obtained from the EPA CERCLIS database for analysis in this study. Data were selected for the fiscal years 1981 to 2009 in four NPL listing status categories: proposal to the NPL, final NPL listing, deletion from the NPL, and achievement of construction completion. Since the Superfund tax expired in 1995, data from the 1981 to 1995 fiscal years and data from the 1996 to 2009 fiscal years were analyzed to determine if there were mean differences …


Cumulative Risks To Eastern Oysters, Crassostrea Virginica In The James River, Va, Vrushali Lele May 2011

Cumulative Risks To Eastern Oysters, Crassostrea Virginica In The James River, Va, Vrushali Lele

Theses and Dissertations

In an effort to apply Cumulative Risk Assessment (CRA) as developed by the U.S. EPA, the present study investigates the cumulative risks to Eastern oysters due to multiple stressors such as salinity, temperature and oxygen and carbon dioxide. I also compared the effectiveness of the Hazard Quotient Method (HQ) in CRA. Ambient conditions in the James River, VA were obtained from the Virginia DEQ database and respiratory responses were estimated using values from the literature. The multiple environmental stresses are evaluated using a probabilistic analysis that combines the environmental conditions. It was concluded that salinity was the most influential stressor …


Phytoremediation Of Pcb Contaminated Soil: Effectiveness And Regulatory Policy, Nathan Pinsker Apr 2011

Phytoremediation Of Pcb Contaminated Soil: Effectiveness And Regulatory Policy, Nathan Pinsker

Theses and Dissertations

The purpose of this paper was to combine available peer-reviewed literature on PCB phytoremediation and make direct comparisons using ANCOVA statistics in order to determine if and what plants are presently a viable option for the remediation of PCB contaminated soils. Pumpkin (Curbita pepo cv Howden) consistently had the top root and shoot concentrations, as well as total plant accumulation. Their consistency shows that they can be reliable in the field and the most viable option. Tall fescue and sedge were also top contenders. Due to the small sample size for many plants and accounting for several confounding variables, very …


Factors Influencing Macroinvertebrate Diversity And Community Composition In Riverine Freshwater Rock Pools, Chtaura Jackson Dec 2010

Factors Influencing Macroinvertebrate Diversity And Community Composition In Riverine Freshwater Rock Pools, Chtaura Jackson

Theses and Dissertations

I examined a system of 378-riverine rock pools situated along the James River in Richmond, Virginia, to uncover the factors influencing their macroinvertebrate diversity and community composition. I characterized (total = 60) the physical characteristics, water quality, hydrology, and community assemblages in July 2009 for a subset of pools. I examined whether selected environmental factors (pH, nearest neighbor distance, depth, surface area and inundation height) were related to macroinvertebrate diversity and community composition. Species richness and total abundance of all taxa increased with pool pH and surface area. Ordination indicates that nearest neighbor distance (NND) and pH varied significantly among …


Phytoremediation Of Copper Contaminated Waste Water Using Lemna Minor, Mark Apelt Nov 2010

Phytoremediation Of Copper Contaminated Waste Water Using Lemna Minor, Mark Apelt

Theses and Dissertations

The use of natural remediation methods to remove contaminants from waste water is becoming more popular. Plants have been used for several decades, yet their use for municipal waste water contaminated by heavy metals is limited to a few studies which focus on the Mercury and Chromium (Bennicelli, et.al, 2004). This study specifically attempted to determine the viability for using Lemna minor to remediate municipally generated wastewater contaminated with copper. The study used 100 ml samples of wastewater, artificially spiked with 8 mg/L of copper sulfate and seeded with approximately 100 Lemna minor fronds. Each treatment was repeated 15 times …


Patterns In Fish Community Structure In A Regulated River, Richard Davis Apr 2010

Patterns In Fish Community Structure In A Regulated River, Richard Davis

Theses and Dissertations

I examined the abundance, composition, and distribution of fish communities in the lower Roanoke River, a hydropeaking system in North Carolina. Fishes were sampled at before and after peaking events over three years; 2007 to 2009. I evaluated trends in species richness, diversity, and assemblage composition. There were no significant differences in either richness or diversity suggesting consistent trends in richness and diversity throughout the study. I used non-metric multidimensional scaling (NMDS) to create a community composition model. Fish composition was noticeably greater post-peaking and changed minimally across time and event. There were no statistically significant differences in species composition …


Factors Limiting Benthic Algal Abundance In Virginia Streams Of The Coastal Plain, Michael Brandt Jul 2009

Factors Limiting Benthic Algal Abundance In Virginia Streams Of The Coastal Plain, Michael Brandt

Theses and Dissertations

Algae are important components of stream food webs and often used in biomonitoring assessments. Little is known regarding the factors that limit their abundance in streams of the VA Coastal Plain. The surficial geology of the Coastal Plain is predominately sandy deposits which comprise the dominant substrate in streams of this region. In a comparative study of five streams located near the VCU Rice Center, we quantified substrate composition, light availability, and nutrient concentrations to assess their relative importance in determining benthic algal abundance. The proportion of stream area comprised of hard substrates was a significant predictor of variation in …


Shrinking The Janzen-Connell Doughnut: Consequences Of An Invasive Multiplier (Microstegium Vimineum) On The Mid-Canopy In A Mixed Pine-Oak Forest, Rebekha Shaw May 2009

Shrinking The Janzen-Connell Doughnut: Consequences Of An Invasive Multiplier (Microstegium Vimineum) On The Mid-Canopy In A Mixed Pine-Oak Forest, Rebekha Shaw

Theses and Dissertations

Introduction of invasive species can alter seed fate predictions made by the Janzen-Connell Escape Hypothesis (JCEH). The JCEH states that there is a suitable region around a plant that is ideal for seed germination, growth, and recruitment. Seeds dispersed too close to the maternal plant are subject to competition from the maternal individual and perhaps density-dependent predation, whereas seeds dispersed further away may end up in suboptimal habitats. Invasive species may change the amount of these suitable habitats for native plants by creating unsuitable light environments and as a result, may influence the size of the ideal recruitment zone surrounding …


Habitat Suitability Criteria For Fishes Of The South Fork Of The Shenandoah River And An Investigation Into Observer Effects Associated With Two Techniques Of Direct Underwater Observation, Robert Clayton Ramey Apr 2009

Habitat Suitability Criteria For Fishes Of The South Fork Of The Shenandoah River And An Investigation Into Observer Effects Associated With Two Techniques Of Direct Underwater Observation, Robert Clayton Ramey

Theses and Dissertations

This study constructed habitat suitability criteria for fishes of the South Fork of the Shenandoah River, in Virginia. The criteria will be used in an IFIM study to produce estimates of the discharge required by fishes in the South Fork. Chi-square tests were used to evaluate whether criteria described habitat use to a statistically significant degree. Secondly, chi-square tests were used to test transferability. The criteria described the habitat use of seven taxa commonly found in the South Fork to a statistically significant degree. Habitat criteria for two taxa did not describe their habitat use to a statistically significant degree. …


An Environmental And Economic Assessment Of Future Municipal Solid Waste Disposal: A Case Study Of Select High Growth Regions Of Virginia., Joseph Krouse Apr 2009

An Environmental And Economic Assessment Of Future Municipal Solid Waste Disposal: A Case Study Of Select High Growth Regions Of Virginia., Joseph Krouse

Theses and Dissertations

This research analyzed environmental and economic factors associated with municipal solid waste (MSW) management of select high growth Planning District Commissions (PDCs) of Virginia. Current MSW management scenarios were compared to future hypothetical scenarios utilizing a regional landfill or waste-to-energy (WTE) combustion facility. Life-cycle inventory and full cost accounting methods of the Municipal Solid Waste Decision Support Tool, developed by the Environmental Protection Agency (EPA), were utilized to estimate annualized environmental emissions and economic costs. Model results and analysis indicate that a regional landfill would be the least cost intensive MSW management strategy in comparison to current management methods; however …


Hyperspectral Reflectance For Rapid Viability Assessment Of Bacillus Endospores, Jarrod Edwards Apr 2009

Hyperspectral Reflectance For Rapid Viability Assessment Of Bacillus Endospores, Jarrod Edwards

Theses and Dissertations

A study was conducted to optically determine the viability of the Gram +, endospore-forming bacterial genera Bacillus using a hyperspectral reflectance spectrometer. Endospores are a dormant, differentiated cellular capsule form taken by select bacteria to ensure survival when environmental conditions become unfavorable. Two species of Bacillus were used for this study, Bacillus megaterium and Bacillus subtilis. These endospores were killed using a chemical treatment of sodium hypochlorite or a physical treatment of heat and pressure in an autoclave. The treated samples along with viable samples were lyophilized to form a powder. A reflectance spectrometer measuring 350 nm to 2500 nm …


Elemental Composition And Nutrient Effect On The Uptake And Metabolism Of Dissolved Organic Carbon By Bacteria From A Temperate Region River, Anne Stuart Apr 2009

Elemental Composition And Nutrient Effect On The Uptake And Metabolism Of Dissolved Organic Carbon By Bacteria From A Temperate Region River, Anne Stuart

Theses and Dissertations

Rivers are arteries that connect land and sea, and provide a conduit and reactor for allochthonous and autochthonous organic carbon sources (OC) delivered to the coastal ocean. In comparison to marine waters, inland waters quantitatively represent only a fraction of the marine system; however, their importance to global C cycling maybe disproportional to its actual size. Inland systems are subject to multiple sources of OC (autochthonous and allochthonous) that vary individually in space and time with respect to their concentration and potential bacterial bioavailability. This study investigates the impact of high and ambient inorganic nutrient concentrations on the bacterial bioavailability …


The Responses Of Blue Crabs (Callinectes Sapidus) To Hypoxia/Hypercapnia In Freshwater, James Martin Apr 2009

The Responses Of Blue Crabs (Callinectes Sapidus) To Hypoxia/Hypercapnia In Freshwater, James Martin

Theses and Dissertations

The present research examined respiratory responses of blue crabs to long term (4, 13, and 21 days) hypercapnic hypoxia in freshwater at 23 C. Hypoxic conditions (50-60 & 75-85 mmHg O2) were induced by allowing the crabs to consume their oxygen supply, resulting in a hypercapnic induced decrease in pH that remained through the exposure. Postbranchial hemolymph responses to hypoxia/hypercapnia in freshwater demonstrate decreases in PO2, increases in PCO2, and decreases in pH. Lactate levels decreased over time, but hemocyanin concentration was highly variable with no trends. PH, lactate, and hemocyanin observations also demonstrated high variability and a variety of …


Role Of Bcl-2 Family Members To Promote Glucocorticoid –Induced Apoptosis By Mek Inhibitors In Leukemic Cells, Anila Rambal Apr 2009

Role Of Bcl-2 Family Members To Promote Glucocorticoid –Induced Apoptosis By Mek Inhibitors In Leukemic Cells, Anila Rambal

Theses and Dissertations

Glucocorticoids (GC) are common components of many chemotherapeutic regimens for lymphoid malignancies. GC-induced apoptosis involves an intrinsic BCL-2 family-regulated pathway. It has been shown that BIM (BCL-2 interacting mediator of cell death), a BH3-only pro-apoptotic protein, is up-regulated by dexamethasone (Dex) treatment in acute lymphoblastic leukemia (ALL) cells. Furthermore, BIM is inactivated by extracellular signal-regulated kinase (ERK)-mediated phosphorylation. We therefore hypothesized co-treatment with Dex and MEK/ERK inhibitors would promote apoptosis in ALL cells through BIM up-regulation and activation. We show here that a MEK inhibitor, PD184352 synergistically enhances Dex lethality in CCRF-CEM (T-ALL) cells. Co-treatment with Dex and PD184352 results …


Patterns Of Abundance Across An Urban-Rural Gradient For Commonly Found Indigenous Arthropods, David Jones Apr 2009

Patterns Of Abundance Across An Urban-Rural Gradient For Commonly Found Indigenous Arthropods, David Jones

Theses and Dissertations

Abstract Proof of concept for a continuous environmental sampling methodology that employs common terrestrial arthropods as environmental samplers was tested by analyzing pitfall, malaise and black light captures over a six month period over a replicated urban-suburban-rural gradient in Central Virginia. All arthropods captured at the nine sites were identified and assigned to aquatic, vegetation, or soil groups based on their association with these microhabitats. To offset variability in arthropod life history patterns and species abundance within habitat types, arthropod categories based on presence/absence data over the six month period were constructed to provide for sampling reliability within each microhabitat …


Determination Of Size Fractions And Concentrations Of Airborne Particulate Matter Generated From Construction And Demolition Waste Processing Facilities, Jason M. Young Jan 2007

Determination Of Size Fractions And Concentrations Of Airborne Particulate Matter Generated From Construction And Demolition Waste Processing Facilities, Jason M. Young

Theses and Dissertations

Construction and Demolition (C&D) processing and recycling facilities accept waste materials that are generated during construction and demolition activities. The processing facility sorts, processes, and transfers the material to another operation. A consequence of these processes is the generation of particulate matter.This study involved quantifying and qualifying airborne particulates at three C&D processing facilities. Active stationary particulate sampling devices were employed to quantify the particle sizes of interest. Results were compared to United States Environmental Protection Agency (EPA) National Ambient Air Quality Standards(NAAQS) and United States Occupational Safety and Health Administration (OSHA)standards.The results from this study are not valid for …


An Evaluation Of Mold In Public Schools In The City Of Richmond, Va, Stephen Asante-Ansong Jan 2007

An Evaluation Of Mold In Public Schools In The City Of Richmond, Va, Stephen Asante-Ansong

Theses and Dissertations

Forty-three (43) schools in the City of Richmond were used for this study. The rooms in these schools that were selected for testing were those rooms in which complaints about air quality were made by school staff. Tests were done to find out the counts of the different mold species present in these schools. Air-O-Cell (AOC) samples were taken in all schools, swab samples were taken in a few and in the rest biotapes were used. Samples that were taken were analyzed and interpreted at AmeriSci Laboratories, an accredited industrial hygiene laboratory. Documentation was done for the sampling methods. Statistical …


An Assessment Of Impediments To Low-Impact Development In The Virginia Portion Of The Chesapeake Bay Watershed, Rebecca V'Lent Lassiter Jan 2007

An Assessment Of Impediments To Low-Impact Development In The Virginia Portion Of The Chesapeake Bay Watershed, Rebecca V'Lent Lassiter

Theses and Dissertations

Stormwater runoff from urban and urbanizing areas poses a serious threat to water quality, and unless managed properly will impede efforts to restore the Chesapeake Bay.Water quantity, as well as quality, must be considered, and Low Impact Development(LID) is an innovative stormwater management approach that addresses both. LID seeks to mimic a site's predevelopment hydrologic regime by retaining and treating stormwater at the lot level using small, cost-effective landscape features.The purpose for this study was to identify and rank impediments to the implementation of LID in the Virginia portion of the Chesapeake Bay watershed. This was accomplished by going to …


A Gis And Remote Sensing Based Analysis Of Impervious Surface Influences On Bald Eagle (Haliaeetus Leucocephalus) Nest Presence In The Virginia Portion Of The Chesapeake Bay, Jennifer M. Ciminelli Jan 2006

A Gis And Remote Sensing Based Analysis Of Impervious Surface Influences On Bald Eagle (Haliaeetus Leucocephalus) Nest Presence In The Virginia Portion Of The Chesapeake Bay, Jennifer M. Ciminelli

Theses and Dissertations

GIS (Geographic Information Systems) and remote sensing techniques were used to predict relationships between bald eagle nest presences and land type, distance to land type and impervious surface cover area. Data plots revealed bald eagle nest presence decreases in response to an increase in area of bareland; increases with an increase in area of forested land; decreases with an increase in distance (m) to shoreline, and decreases in response to an increase in area of impervious surfaces. Logistic regression models identified impervious surfaces as an indicator for bald eagle nest presence (P 24% as unsuitable. Unsuitable area covered 17.82% of …


Isolation Of Legionella Pneumophila From Well-Maintained Emergency Showers And Eyewash Stations, Jessica Mae Myers Jan 2006

Isolation Of Legionella Pneumophila From Well-Maintained Emergency Showers And Eyewash Stations, Jessica Mae Myers

Theses and Dissertations

Legionella pneumophila is a gram-negative bacterium responsible for Legionnaire's disease, and is commonly transmitted via aerosolized water. Legionella colonization of emergency eyewash and shower stations may pose an exposure hazard to users of these stations. There is little information about the role of these stations as significant reservoirs for Legionella. Samples were collected from 67 stations in an industrial facility. At the time of this study, the stations within this facility were under a routine maintenance program that included at least monthly flushing. This study also included the analysis for other bacterial organisms to determine an association between the presence …


Sub-Lethal Effects Of Hypoxia/Hypercapnia On Callinectes Sapidus In The York River Estuary, Virginia, Sandra R. Hypes Jan 1999

Sub-Lethal Effects Of Hypoxia/Hypercapnia On Callinectes Sapidus In The York River Estuary, Virginia, Sandra R. Hypes

Theses and Dissertations

This research examined effects of hypoxic environments on blue crabs, Callinectes sapidus in an estuarine environment. Hypoxic conditions were treated as a multiple stressor involving low dissolved oxygen (D.O.), increased carbon dioxide (hypercapnia), and low pH concurrently. The objectives were to: 1) identify hypoxiahypercapnia by monitoring D.O. and pH as an indicator of hypercapnia in shallow regions of the York River, 2) measure blue crab abundance, and 3) describe blue crab responses to hypoxiahypercapnia via field work at Taskinas Creek and lab measurements of respiration. Ambient D.O. and pH were positively correlated in the Taskinas Creek and York River sites …