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Land Use Influences The Composition And Antimicrobial Effects Of Propolis, Amara J. Orth, Emma H. Curran, Eric J. Haas, Andrew C. Kraemer, Audrey M. Anderson, Nicholas J. Mason, Carol A. Fassbinder-Orth Feb 2022

Land Use Influences The Composition And Antimicrobial Effects Of Propolis, Amara J. Orth, Emma H. Curran, Eric J. Haas, Andrew C. Kraemer, Audrey M. Anderson, Nicholas J. Mason, Carol A. Fassbinder-Orth

Department of Biological Systems Engineering: Papers and Publications

Honey bee propolis is a complex, resinous mixture created by bees using plant sources such as leaves, flowers, and bud exudates. This study characterized how cropland surrounding apiaries affects the chemical composition and antimicrobial effects of propolis. The chemical composition and compound abundance of the propolis samples were analyzed using Gas Chromatography- Mass Spectrometry (GC-MS) and the antimicrobial effects were analyzed using the 50% minimum inhibitory concentration (MIC50) assay against four relevant bee pathogens, Serratia marcescens, Paenibacillus larvae, Lysinibacillus sphaericus, and Klebsiella pneumoniae. Propolis composition varied significantly with apiary, and cropland coverage predicted mean sum abundance of …


Fate Of Antimicrobials And Antimicrobial Resistance Genes In Simulated Swine Manure Storage, Stacey R. Joy, Xu Li, Daniel D. Snow, John Gilley, Bryan L. Woodbury, Shannon L. Bartelt-Hunt Jan 2014

Fate Of Antimicrobials And Antimicrobial Resistance Genes In Simulated Swine Manure Storage, Stacey R. Joy, Xu Li, Daniel D. Snow, John Gilley, Bryan L. Woodbury, Shannon L. Bartelt-Hunt

School of Natural Resources: Faculty Publications

The behavior of three antibiotics (bacitracin, chlortetracycline, and tylosin) and two classes of antibiotic resistance genes (ARGs), tet and erm, were monitored in swine manure slurry under anaerobic conditions. First-order decay rates were determined for each antibiotic with half-lives ranging from1 day (chlortetracycline) to 10 days (tylosin). ARGs were monitored in the swine manure slurry, and losses of approximately 1 to 3 orders of magnitude in relative abundance were observed during the 40 day storage period. First-order degradation profiles were observed for chlortetracycline and its corresponding resistance genes, tet(X) and tet(Q). Tylosin was degraded to approximately 10% …


Narrow Grass Hedge Effects On The Transport Of Antimicrobials And Antimicrobial Resistance Genes Following Land Application Of Swine Slurry, Bhavneet Soni Dec 2013

Narrow Grass Hedge Effects On The Transport Of Antimicrobials And Antimicrobial Resistance Genes Following Land Application Of Swine Slurry, Bhavneet Soni

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

The objective of this study was to determine the effects of manure amendment and narrow grass hedges on the fate and transport of antimicrobials and ARGs in runoff and in soil following the land application of swine manure slurry. Swine manure slurry was land applied to 0.75m wide by 4.0m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. The treatment factor manure amendment consisted of two levels: no manure application and manure application to meet the 3 year nitrogen (N) requirements for corn. The treatment factor of grass hedge was established for half of …