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- Department of Civil and Environmental Engineering: Faculty Publications (3)
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Articles 1 - 14 of 14
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Use Of Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba) To Mitigate Salmonella Enterica, Shiga Toxin-Producing Escherichia Coli (Stec) And Aspergillus Flavus In Pet Food Kibbles, Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Use Of Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba) To Mitigate Salmonella Enterica, Shiga Toxin-Producing Escherichia Coli (Stec) And Aspergillus Flavus In Pet Food Kibbles, Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Department of Food Science and Technology: Faculty Publications
Post-processing operations of extruded pet food kibbles involve coating the product with fats and flavorings. These processes increase the risk for cross-contamination with food-borne pathogens such as Salmonella and Shiga toxin-producing Escherichia coli (STEC), and mycotoxin-producing molds such as Aspergillus spp. after the thermal kill step. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WD-MAX™, against Salmonella enterica, STEC and Aspergillus flavus when used as a coating on pet food kibbles were evaluated. Using canola oil and dry dog digest as fat and flavor …
Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Department of Food Science and Technology: Faculty Publications
Contaminated surfaces can transmit pathogens to food in industrial and domestic food-handling environments. Exposure to pathogens on food contact surfaces may take place via the cross-contamination of pathogens during postprocessing activities. Formaldehyde-based commercial sanitizers in recent years are less commonly being used within food manufacturing facilities due to consumer perception and labeling concerns. There is interest in investigating clean-label, food-safe components for use on food contact surfaces to mitigate contamination from pathogenic bacteria, including Salmonella. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WDMAX …
Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Mitigation Of Salmonella On Food Contact Surfaces By Using Organic Acid Mixtures Containing 2-Hydroxy-4-(Methylthio) Butanoic Acid (Hmtba), Aiswariya Deliephan, Janak Dhakal, Bhadriraju Subramanyam, Charles G. Aldrich
Department of Food Science and Technology: Faculty Publications
Contaminated surfaces can transmit pathogens to food in industrial and domestic food-handling environments. Exposure to pathogens on food contact surfaces may take place via the cross-contamination of pathogens during postprocessing activities. Formaldehyde-based commercial sanitizers in recent years are less commonly being used within food manufacturing facilities due to consumer perception and labeling concerns. There is interest in investigating clean-label, food-safe components for use on food contact surfaces to mitigate contamination from pathogenic bacteria, including Salmonella. In this study, the antimicrobial effects of two types of organic acid mixtures containing 2-hydroxy-4-(methylthio) butanoic acid (HMTBa), Activate DA™ and Activate US WD-MAX™, …
Land Use Influences The Composition And Antimicrobial Effects Of Propolis, Amara J. Orth, Emma H. Curran, Eric J. Haas, Andrew C. Kraemer, Audrey M. Anderson, Nicholas J. Mason, Carol A. Fassbinder-Orth
Land Use Influences The Composition And Antimicrobial Effects Of Propolis, Amara J. Orth, Emma H. Curran, Eric J. Haas, Andrew C. Kraemer, Audrey M. Anderson, Nicholas J. Mason, Carol A. Fassbinder-Orth
Department of Biological Systems Engineering: Papers and Publications
Honey bee propolis is a complex, resinous mixture created by bees using plant sources such as leaves, flowers, and bud exudates. This study characterized how cropland surrounding apiaries affects the chemical composition and antimicrobial effects of propolis. The chemical composition and compound abundance of the propolis samples were analyzed using Gas Chromatography- Mass Spectrometry (GC-MS) and the antimicrobial effects were analyzed using the 50% minimum inhibitory concentration (MIC50) assay against four relevant bee pathogens, Serratia marcescens, Paenibacillus larvae, Lysinibacillus sphaericus, and Klebsiella pneumoniae. Propolis composition varied significantly with apiary, and cropland coverage predicted mean sum abundance of …
Net Return Distributions When Metaphylaxis Is Used To Control Bovine Respiratory Disease In High Health-Risk Cattle, Elliott James Dennis, Ted C. Schroeder, David G. Renter
Net Return Distributions When Metaphylaxis Is Used To Control Bovine Respiratory Disease In High Health-Risk Cattle, Elliott James Dennis, Ted C. Schroeder, David G. Renter
Department of Agricultural Economics: Faculty Publications
This study’s objective was to estimate net returns and return risk for antimicrobial metaphylaxis options to manage bovine respiratory disease (BRD) in high health-risk feedlot cattle. The effectiveness of antimicrobials for metaphylaxis varies by cattle population. How differing antimicrobial effectiveness translates to net return profitability for heterogeneous cattle populations is less understood. Net returns and return risk were assessed using a net return simulation model adapted to allow for heterogeneity in high health-risk cattle placement characteristics and antimicrobial choice to control BRD. The net return model incorporated how antimicrobials modify BRD health and performance outcomes. Health and performance outcomes were …
Prophage Induction Reduces Shiga Toxin Producing Escherichia Coli (Stec) And Salmonella Enterica On Tomatoes And Spinach: A Model Study, Brigitte Cadieux, Anna Colavecchio, Julie Jeukens, Luca Freschi, Jean-Guillaume Emond-Rheault, Irena Kukavica-Ibrulj, Roger C. Levesque, Sakjia Bekal, Jeffrey C. Chandler, Shannon M. Coleman, Bledar Bisha, Lawrence D. Goodridge
Prophage Induction Reduces Shiga Toxin Producing Escherichia Coli (Stec) And Salmonella Enterica On Tomatoes And Spinach: A Model Study, Brigitte Cadieux, Anna Colavecchio, Julie Jeukens, Luca Freschi, Jean-Guillaume Emond-Rheault, Irena Kukavica-Ibrulj, Roger C. Levesque, Sakjia Bekal, Jeffrey C. Chandler, Shannon M. Coleman, Bledar Bisha, Lawrence D. Goodridge
USDA Wildlife Services: Staff Publications
Fresh produce is increasingly implicated in foodborne outbreaks and most fresh produce is consumed raw, emphasizing the need to develop non-thermal methods to control foodborne pathogens. This study investigates bacterial cell lysis through induction of prophages as a novel approach to control foodborne bacterial pathogens on fresh produce. Shiga toxin producing Escherichia coli (STEC) and Salmonella enterica isolates were exposed to different prophage inducers (i.e. mitomycin C or streptonigrin) and growth of the cells was monitored by measuring the optical density (OD600) during incubation at 37C. Beginning at three hours after addition of the inducer, all concentrations (0.5, …
Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt
Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt
Department of Civil and Environmental Engineering: Faculty Publications
The use of wastewater for irrigation may introduce antimicrobials and human pathogens into the food supply through vegetative uptake. The objective of this study was to investigate the uptake of three antimicrobials and Salmonella in two lettuce cultivars. After repeated subirrigation with synthetic wastewater, lettuce leaves and soil were collected at three sequential harvests. The internalization frequency of Salmonella in lettuce was low. A soil horizon-influenced Salmonella concentration gradient was determined with concentrations in bottom soil 2 log CFU/g higher than in top soil. Lincomycin and sulfamethoxazole were recovered from lettuce leaves at concentrations as high as 822 ng/g and …
Influence Of Grass Hedges On The Transport Of Antimicrobials, Antimicrobial Resistance Genes, And Antimicrobial Resistant Pathogens After Land Application Of Swine Manure, Shannon L. Bartelt-Hunt
Influence Of Grass Hedges On The Transport Of Antimicrobials, Antimicrobial Resistance Genes, And Antimicrobial Resistant Pathogens After Land Application Of Swine Manure, Shannon L. Bartelt-Hunt
Department of Civil and Environmental Engineering: Faculty Publications
The objective of this study was to determine the effects of narrow grass hedges on the fate and transport of antimicrobials and antimicrobial resistance genes (ARGs) in runoff and in soil following the land application of swine manure slurry. Swine manure slurry was land applied to 0.75m wide by 4.0m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. Swine manure was applied at a rate to meet the 3-year nitrogen (N) requirements for corn. Swine manure was applied to plots with and without a narrow grass hedge to evaluate the effect of the hedge …
Fate Of Antimicrobials And Antimicrobial Resistance Genes In Simulated Swine Manure Storage, Stacey R. Joy, Xu Li, Daniel D. Snow, John Gilley, Bryan L. Woodbury, Shannon L. Bartelt-Hunt
Fate Of Antimicrobials And Antimicrobial Resistance Genes In Simulated Swine Manure Storage, Stacey R. Joy, Xu Li, Daniel D. Snow, John Gilley, Bryan L. Woodbury, Shannon L. Bartelt-Hunt
School of Natural Resources: Faculty Publications
The behavior of three antibiotics (bacitracin, chlortetracycline, and tylosin) and two classes of antibiotic resistance genes (ARGs), tet and erm, were monitored in swine manure slurry under anaerobic conditions. First-order decay rates were determined for each antibiotic with half-lives ranging from1 day (chlortetracycline) to 10 days (tylosin). ARGs were monitored in the swine manure slurry, and losses of approximately 1 to 3 orders of magnitude in relative abundance were observed during the 40 day storage period. First-order degradation profiles were observed for chlortetracycline and its corresponding resistance genes, tet(X) and tet(Q). Tylosin was degraded to approximately 10% …
Narrow Grass Hedge Effects On The Transport Of Antimicrobials And Antimicrobial Resistance Genes Following Land Application Of Swine Slurry, Bhavneet Soni
Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research
The objective of this study was to determine the effects of manure amendment and narrow grass hedges on the fate and transport of antimicrobials and ARGs in runoff and in soil following the land application of swine manure slurry. Swine manure slurry was land applied to 0.75m wide by 4.0m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. The treatment factor manure amendment consisted of two levels: no manure application and manure application to meet the 3 year nitrogen (N) requirements for corn. The treatment factor of grass hedge was established for half of …
Occurrence Of Antimicrobials And Antimicrobial Resistance Genes In Beef Cattle Storage Ponds And Swine Treatment Lagoons, Yuping Zhang, Chiqian Zhang, David B. Parker, Daniel D. Snow, Zhi Zhou, Xu Li
Occurrence Of Antimicrobials And Antimicrobial Resistance Genes In Beef Cattle Storage Ponds And Swine Treatment Lagoons, Yuping Zhang, Chiqian Zhang, David B. Parker, Daniel D. Snow, Zhi Zhou, Xu Li
Nebraska Water Center: Faculty Publications
Livestock manure treatment and storage structures are potential environmental sources of antimicrobials and antimicrobial resistance genes (ARGs). In this study, the occurrence of antimicrobials and ARGs was investigated in the water and the sludge compartments of beef cattle storage ponds and swine lagoons. Analysis was focused on two families of antimicrobials (sulfonamide and tetracycline) and the corresponding ARGs (sul1, sul2, tetO, tetQ and tetX). Results showed that the pseudo-partitioning coefficients of tetracyclines were higher than those of sulfonamides, suggesting different distributions of these two classes of antimicrobials between water and sludge. The ARGs tested were …
Effects Of The Antimicrobial Tylosin On The Microbial Community Structure Of An Anaerobic Sequencing Batch Reactor, Toshio Shimada, Xu Li, Julie L. Zilles, Eberhard Morgenroth, Lutgarde Raskin
Effects Of The Antimicrobial Tylosin On The Microbial Community Structure Of An Anaerobic Sequencing Batch Reactor, Toshio Shimada, Xu Li, Julie L. Zilles, Eberhard Morgenroth, Lutgarde Raskin
Department of Civil and Environmental Engineering: Faculty Publications
The effects of the antimicrobial tylosin on a methanogenic microbial community were studied in a glucosefed laboratory-scale anaerobic sequencing batch reactor (ASBR) exposed to stepwise increases of tylosin (0, 1.67, and 167 mg/L). The microbial community structure was determined using quantitative fluorescence in situ hybridization (FISH) and phylogenetic analyses of bacterial 16S ribosomal RNA (rRNA) gene clone libraries of biomass samples. During the periods without tylosin addition and with an influent tylosin concentration of 1.67 mg/L, 16S rRNA gene sequences related to Syntrophobacter were detected and the relative abundance of Methanosaeta species was high. During the highest tylosin dose of …
Cationic Polypeptides Contribute To The Anti-Hiv-1 Activity Of Human Seminal Plasma, Julie A. Martellini, Amy L. Cole, Nitya Venkataraman, Gerry A. Quinn, Pavel Svoboda, Bhushan K. Gangrade, Jan Pohl, Ole E. Sorensen, Alexander M. Cole
Cationic Polypeptides Contribute To The Anti-Hiv-1 Activity Of Human Seminal Plasma, Julie A. Martellini, Amy L. Cole, Nitya Venkataraman, Gerry A. Quinn, Pavel Svoboda, Bhushan K. Gangrade, Jan Pohl, Ole E. Sorensen, Alexander M. Cole
Public Health Resources
Mucosal surfaces of the reproductive tract as well as their secretions have important roles in preventing sexual transmission of HIV-1. In the current study, the majority of the intrinsic anti-HIV-1 activity of human seminal plasma (SP) was determined to reside in the cationic polypeptide fraction. Antiviral assays utilizing luciferase reporter cells and lymphocytic cells revealed the ability of whole SP to prevent HIV-1 infection, even when SP was diluted 3200-fold. Subsequent fractionation by continuous flow acid-urea (AU)- PAGE and antiviral testing revealed that cationic polypeptides within SP were responsible for the majority of anti-HIV-1 activity. A proteomic approach was utilized …
G85-748 Prevention And Control Of Swine Dysentery, Gerald E. Duhamel, Alex Hogg, Barbara E. Straw
G85-748 Prevention And Control Of Swine Dysentery, Gerald E. Duhamel, Alex Hogg, Barbara E. Straw
University of Nebraska-Lincoln Extension: Historical Materials
This NebGuide describes the causes and symptoms of swine dysentery and offers management recommendations and treatment procedures for its prevention and control.
Swine dysentery is a highly contagious disease of growing and finishing pigs. First described in Indiana in 1921, it has been called black scours, bloody scours, and vibrionic dysentery.
Swine dysentery causes important financial losses because of reduced feed efficiency and lower weight gain, costs of medication and additional animal care, and death. Substantial costs may result from loss of sales of breeding stock, or depopulation when necessary. Serpula (Treponema) hyodysenteriae, a spiral bacterium, is the cause …