Digital Commons Network^™

Detection Of Early Stages Of Myxobolus Cerebralis In Fin Clips From Rainbow Trout (Onchorynchus Mykiss), Ramona T. Skirpstunas, J. Hergert, Thomas J. Baldwin

Animal, Dairy, and Veterinary Science Faculty Publications

A nested polymerase chain reaction (PCR) assay was used to detect early stages of Myxobolus cerebralis in caudal and adipose fin samples from rainbow trout (RT). To determine sensitivity, groups of 10 RT were exposed to 2,000 M. cerebralis triactinomyxons/fish for 1 hour at 15 degrees C and subsequently moved to clean recirculating water. Fish were held for 2 and 6 hours and 1, 2, 3, 5, 7, 10, 30, and 60 days before sampling by nonlethal fin biopsy. Nested PCR performed on fin clips showed that M. cerebralis DNA was detected in caudal fin tissue in 100% of fish …