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Biological Seawater Desalination Using Green Algae And Cyanobacteria, Abdul Mannan Zafar

Dissertations

Water shortage in many coastal parts of the world is reaching alarming situation, including the United Arab Emirates. Urbanization and rapid population growth are generating critical water demands with an increase in per capita consumption. Seawater is usually treated by membrane processes such as Reverse Osmosis (RO). However, this technology is energy intensive and generates excessive brine as a waste. The solution to this problem can be found in natural remediation techniques that can reduce salt concentration from seawater, such as halophytes. A salt-tolerant species can consume Na⁺ and Cl^- ions and increase cellular growth which is termed …

Reasons And Remedies To The Dying Plant Walls, Hayley Q. Mcgough

Construction Management

Sustainability in construction has been increasingly popular and detrimental with the state of the planet and climate change. One of the more popular solutions is the addition of plant façades to a building, which are plants that are put on the vertical of a building. The benefits that come with plant facades include and are not limited to: Reduction of the urban heat island effect, improved air quality, local job creation, improved energy efficiency, building structure protection, noise reduction, increased biodiversity, urban agriculture. These benefits are not reaching their full potential because these plant façades keep dying creating a bigger …

Adaptive Laboratory Evolution Of Scenedesmus Obliquus For Increased Carbohydrate Content And Biomass Productivity, Nahel Ali

Master's Theses

The economics of microalgal bioproduct commercialization would benefit from increased accumulation of energy storage compounds, such as carbohydrates and lipids, and increased biomass productivity. This thesis explores two adaptive laboratory evolution strategies for improving Scenedesmus obliquus: single UV-mutagenesis and low light cultivation at a high dilution rate to produce cultigens with greater carbohydrate content and iterative UV-mutagenesis and selection under outdoor conditions in a raceway pond at a high dilution rate to increase biomass productivity.

Two cultigens were generated with the goal of increased carbohydrate content: K5 and K7. Both were mutagenized for 5 seconds and cultivated in 50-mL …

Evaluating And Predicting The Risk Of Algal Blooms In A Freshwater Lake Through A 4-Dimensional Approach: A Case Study On Lake Mitchell, Sumit Kumar Ghosh

Electronic Theses and Dissertations

Excessive algal growth in freshwater lakes can negatively impact ecosystems, recreation, and human health. Though algae are a natural part of freshwater ecosystems, elevated nutrient loading from anthropogenic and natural sources can lead to algal blooms. Both algae and blue-green algae (BGA) are responsible for algal blooms; however, BGA (cyanobacteria) is more dangerous. The first objective of this research was to prepare a conceptual model to understand how various environmental variables affect algae. This conceptual model was used to choose the environmental variables that help increase or decrease algae in the water environment. The second objective was to develop empirical …

Same Stone, Different Outcomes: Marine Communities On Engineered Vs. Natural Rock Shores, Lucy Anne Dando Lockwood

Graduate Masters Theses

The effort to protect coastal property and infrastructure from storm damage, erosion, and sea level rise has resulted in increased construction of coastal protection structures (CPS) worldwide. Researchers around the globe have found that the marine communities living on CPS differ from those living on natural rock outcroppings in the same area. We conducted a classic disturbance experiment to investigate possible differences in marine organism response and community assembly between natural and human-constructed rocky intertidal habitat along the Massachusetts coast. The one-year study used naturally occurring rock shores and human-made granite seawalls with both wave-exposed and wave-protected areas. Significant differences …

Methods To Increase Algae Biomass Productivity In Raceway Pond Monocultures, Ryan S. Anderson

Master's Theses

The economics of algae biofuels and bioproducts would be improved by increased biomass productivity. Two studies on this potential are described in this thesis – one on a locally isolated filamentous yellow-green alga and the other on a planktonic strain genetically improved via selective enrichment.

Polycultures have been viewed as productive, stable, and, in some cases, harvestable by natural bioflocculation. Local native strains might have higher productivity than culture collection strains because they are already adapted to local outdoor conditions. In this study, the filamentous yellow-green alga Tribonema minus was isolated from a local volunteer polyculture. Its productivity as a …

The Combined Toxic Effect Of Nanoparticles And Lead In The Presence Of Algae (Raphidocelis), Xuesong Liu

Doctoral Dissertations

"The elevated toxicity of toxic metals in the presence of nanoparticles (NPs) has raised significant concerns on the NP environmental safety. This research focuses on the impact of environmental conditions, specifically algae, on this type of toxicity. An indicator aquatic organism, Ceriodaphnia dubia (C. dubia), was used to examine the combined toxicity and toxicity mitigation from algae by monitoring its 24-h mortality. Four papers are included in this research, which focus on four aspects of the combined toxicity and the corresponding algae (Raphidocelis) impact. The first paper examines the algae impact on the combined toxicity of …

Integrated Aeration For Enhanced Nitrogen Conversion And Removal And The Effect Of Sampling Frequency On Productivity Estimates In Algae-Based Wastewater Treatment Systems, Lauren Parker

Master's Theses

Raceway ponds can be used to cultivate microalgae for purposes such as wastewater treatment and feedstock for animal feeds and biofuels. Besides nitrogen assimilation in biomass, nitrification can be a wastewater treatment mechanism but one that is hindered by the low nighttime dissolved oxygen concentrations (DO) common in wastewater treatment raceways. The large diel difference in pond conditions and changing influent wastewater quality also raises the question of the optimal frequency and timing of grab sample collection, to obtain representative water quality data. Three sets of triplicate pilot raceways (1,000-L, 0.30-m deep, fed primary clarifier effluent) were operated at an …

Wastewater Treatment And Nitrogen Removal In Bench-Scale Photobioreactors Operated With Solids Recycling, Erik Hoffnagle

Master's Theses

This thesis examines the effect of solids recycling on nitrification, organic carbon removal, and algal-bacterial productivity in bench-scale photobioreactors (PBRs) simulating winter pond conditions in San Luis Obispo, California and a 6-acre raceway system in Delhi, California. Two sets of duplicate photobioreactors operated with or without solids recycling were fed primary clarifier effluent (1^oEff) for the first experiment, and facultative pond effluent (FAC) for the second experiment.

In both experiments mean productivity was lower in solids recycled PBRs (3.67 and 2.3 g/m²-day), than in controls without solids recycling (4.15 and 3.9 g/m²-day). When fed …

Growth Of An Algae Polyculture And Dunaliella Tertiolecta In Oilfield Produced Water At A Range Of Salinities And Nutrient Concentrations For Biofuels Production., Thomas C. Hopkins

Civil Engineering ETDs

Growth of algae in saline produced waters (wastewater) from oil and gas production is of interest for potential production of algal biofuels, wastewater treatment and potential reuse or disposal. The objectives of this study were to determine the effects of salt concentrations and nitrogen source (ammonia or nitrate) and concentration on algal growth, lipid production, and nutrient removal to help determine suitability for growth on saline produced waters. Dunaliella tertiolecta (a marine algae) and a polyculture (previously discovered growing in PW) were grown on produced water with a range of salinities and varying nitrogen source and concentration, in a series …

Chloride Salt Inhibition On Lipid Production In Wastewater-Grown Algae For Biofuel Production, Will Richardson

Biological and Agricultural Engineering Undergraduate Honors Theses

Algae are increasingly being recognized as useful organisms for many applications in today’s world. Their ability to remove nitrogen, phosphorus, and trace metals from water while adding oxygen to water makes them an attractive tertiary treatment technology in municipal wastewater treatment facilities. At the same time, algae produce lipids and carbohydrates that are useful for biofuel production, and they are not a human food crop unlike many biofuel feedstocks. In this study the effect of increased chloride concentrations in wastewater was assessed on the ability of two species of algae, Chlorella vulgaris and Scenedesmus dimorphus, to function as a …

Origins And Seasonal Variation Of Disinfection Byproduct Precursors, Ran Zhao

Doctoral Dissertations

Disinfection byproducts (DBPs) are formed from the disinfectant (e.g., chlorine) reacting with components of natural organic matter (NOM) in water drawn from surface water supplies, and are considered as the cause of potential serious human health problems. DBP precursors originate in large reservoirs from at least three types of sources: (1) watershed or allochthonous, (2) algal or autochthonous, and (3) bottom sediments or benthic. The properties of the NOM and the DBP precursor content of that NOM are unique to each source. The first objective of this dissertation was to use temporal and spatial water quality data from a drinking …

Modeling Canopy Effect In The Great Lakes Cladophora Model, Ankita Bakshi

Dissertations, Master's Theses and Master's Reports

Cladophora glomerata is a filamentous green alga native to the Great Lakes. However, its nuisance growth in phosphorus rich waters negatively affects the lakes’ aesthetic and water quality. The Great Lakes Cladophora Model (GLCM) v1, developed in 1982, was the first mechanistic model to simulate Cladophora growth basing phosphorus availability and environmental conditions followed by Cladophora Growth Model and GLCM v2. In this study, the light and temperature mediation factors for Cladophora net growth are revised as a necessary step prior to the development of a self-shading algorithm. The concept of a fixed-value, maximum achievable biomass (carrying capacity) employed in …

Disinfection By-Products Formation After Coagulation Of Algal Extracellular And Intracellular Organic Matters, Tianyiru Chen

Electronic Thesis and Dissertation Repository

Algal organic matter (AOM) is found in high concentration during algal bloom season in drinking water systems, which is generally categorized into extracellular organic matter (EOM) and intracellular organic matter (IOM). These compounds are not well removed in traditional water treatment methods such as coagulation, and are the precursors of subsequent disinfection by-products (DBPs) during chlorination of water. In this study, EOM and IOM content of four different algae were quantified measuring dissolved organic carbon (DOC), UV absorbance at 254 nm and turbidity. Coagulation using alum (Al₂(SO₄)₃•16H₂O)was used to remove the algal …

Development Of An Anaerobic-Phototrophic Bioreactor System For Wastewater Treatment, Onur Yilmaz Ozcan

USF Tampa Graduate Theses and Dissertations

For decades, mainstream domestic wastewater treatment has relied on activated sludge processes to remove organic matter, and on biological nutrient removal systems like the A²/O process to remove nutrients. Recently, membrane filtration was also added to the realm of possible technologies for domestic wastewater treatment, with aerobic membrane bioreactors (MBRs) becoming increasingly popular, especially for decentralized, and small to medium scale applications. However, the aerobic activated sludge and MBR processes, which are often combined with biological nutrient removal processes, have high energy costs associated with supplying oxygen to the process, and end up converting the organic matter into …

Regrowth Of Chlorella Sorokiniana On Recycled Media With Replenished Nutrients, William H. Spence

Master's Theses

Growth media recycling during algae cultivation is necessary to increase the efficiency and reduce the cost of biofuel production from algae feedstocks. Without recycling media, the cost of algae based biofuel production would be prohibitively high and large scale algae based biofuel production would not be economically viable. The ratio of media recycled to media wasted assumed for algae farms is generally calculated to maintain salt concentrations below growth inhibitory levels, ignoring the influence of secondary metabolites which might decrease productivity. Secondary metabolites, which include allelopathic or auto-inhibitory biological contaminants, might lead to the accumulation of growth-inhibiting compounds in recycled …

Potential For Biofuel Production From Algae Based Wastewater Treatment In California: Can Algal Biofuels Be Cost-Competitive With Traditional Petroleum Based Diesel?, Amanda Rupiper

Master's Projects and Capstones

Neither the use of algae to clean wastewater, nor the use of photosynthetic organisms to generate biodiesel, are new concepts on their own. By combining these two processes, algal-based wastewater treatment with algal biofuel production, additional benefits can be derived, among which could be a cost-savings. In California the average estimated base production cost per gallon for algal biodiesel is $5.98/gallon. Compared to the adjusted production cost of petroleum-based diesel of $1.53/gallon, biodiesel is not cost-competitive. Coupling wastewater treatment and algal cultivation reduces the net energy use of the two processes separately and, if accounted for, greatly reduces the production …

Production And Harvest Of Microalgae In Wastewater Raceways With Resource Recycling, Alexander Colin Roberts

Master's Theses

Microalgae can be grown on municipal wastewater media to both treat the wastewater and produce feedstock for algae biofuel production. However the reliability of treatment must be demonstrated, as well as high areal algae productivity on recycled wastewater media and efficient sedimentation harvesting. This processes was studied at pilot scale in the present research.

A pilot facility was operated with nine CO₂-supplemented raceway ponds, each with a 33-m² surface area and a 0.3-m depth, continuously from March 6, 2013 through September 24, 2014. The ponds were operated as three sets of triplicates with two sets continuously fed …

Comparing Nutrient Recovery Via Rapid (Flash Hydrolysis) And Conventional Hydrothermal Liquefaction Processes For Microalgae Cultivation, Caleb Richard Talbot

Civil & Environmental Engineering Theses & Dissertations

Algal biofuels have the potential to provide a scalable source of renewable fuels in the near future. The high nutrient use in algae cultivation and its recovery and recycling is one of the challenges that may limit the scalability and sustainability of algal biofuels. The present study evaluates the use of Hydrolysate obtained after Flash Hydrolysis (FH) of Scenedesmus at 280 C as a nutrient source for microalgae cultivation. FH Hydrolysate nutrient recycling was compared with low temperature batch Hydrothermal Liquefaction (HTL) nutrient recycling. Oocystis and Scenedesmus were cultivated using Hydrolysate as a partial phosphorous (P) and nitrogen (N) source. …

Nutrient Transformations In Algae Raceway Ponds Fed Municipal Wastewater, Carter Michael Reiff

Master's Theses

While treating domestic wastewater, algae can be grown and harvested for biofuel production. Water is a vital resource and it is imperative to conserve and reuse as much as possible. Several pilot and lab scale experiments were conducted to further research into a full scale wastewater treatment and biofuel production facility. This thesis will include these topics: nitrogen and phosphorus concentrations of clarified wastewater pond feed, nitrogen and phosphorus transformations by algae, and the potential of algae cell disruption technologies to increase nutrient solubilization.

The pilot scale experiments were conducted using nine 33 m², 0.3 m deep multi-culture …

Evaluation Of Point Of Use Drinking Water Treatment Systems For Colonias In The Southwest United States, Isaac Campos Flores

Open Access Theses & Dissertations

Clean drinking water is often taken for granted in first world countries, such as the United States. However, thousands of colonias residents (settlements in the Southwest US that lack access to basic infrastructure) still lack access to clean drinking water. Such is the case in the Paso Del Norte Region. In Doña Ana County, NM, and El Paso County, TX, colonia residents typically rely on shallow domestic wells and hauled water, respectively. However, both water sources can pose health concerns from elevated total dissolved solids and microbiological contamination. With connection to centralized water treatment and distribution still years away, colonias …

Impact Of Algae On Sugar Mill Effluents: Water Treatment And Added Value Assessment, Jacob Stephen Foy

LSU Master's Theses

Installations similar to Louisiana sugar mills have been proposed as potential biorefineries for sugarcane, energy cane, and sweet sorghum. The nutrient-rich wastewater generated from the milling process can be used as an algal growth medium. Simultaneously, algae can serve as a water treatment strategy to deplete these effluents of excess nitrogen, phosphorus, and organic carbon. In this research the effluents of a sugar mill were characterized for water quality and nutrient characteristics; the effluents were determined to be suitable for growth of a native Louisiana co-culture of microalgae and cyanobacteria (Chlorella vulgaris/Leptolyngbya sp.); growth rate, biomass productivity and nutrient uptake …

Treatment Of Nitrogen Oxides By Chlorella Vulgaris Algae In Photobioreactors, Steven Shihady

Master's Theses

The effectiveness of algae to treat NO₂and NO in simulated flue gas was tested using Chlorella vulgaris in photobioreactors (PBRs) using NO_xconcentrations between 30 ppm to 780 ppm. NO_xdissolved and reacted in water to form NO₃^-and NO₂^-in the PBR growth medium, providing a nitrogen source that the algae readily assimilated for cell synthesis. Three 20-L photobioreactors were inoculated with a pure culture of C. vulgaris prepared in Bristol growth medium and algae were grown in the PBRs at 25°C and pH of 7.0 in a modified Bristol medium …

Bioflocculation Of Wastewater Treatment Pond Suspended Solids, Louis Lefebvre

Master's Theses

Bioflocculation of Wastewater Treatment Pond Suspended Solids

Louis Lefebvre

Wastewater treatment lagoons and high rate algae ponds (HRAPs) can provide cost effective wastewater treatment, but they commonly have high effluent concentrations of total suspended solids (TSS). In this thesis algae pond effluent was treated in a beaker testing apparatus (mixed and aerated) with various mixtures of activated sludge and primary effluent simulating differing activated sludge aeration basin compositions then was allowed to settle to assess settleability. Conventionally, microalgal suspended solids are removed by chemical coagulation followed by separation methods that often have a high cost relative to the low cost …

Quantifying Mass Sediment Movement In Deer Creek Reservoir During Spring Runoff And Potential Water Quality Impacts, Colin Rodger Ricks

Theses and Dissertations

The accurate prediction of water quality is essential for management of reservoirs used for drinking water supply. Since algae are a major source of taste and odor problems in drinking water, understanding and controlling algal growth and production is an important task. Deer Creek Reservoir supplies drinking water for over one million people in northern Utah and has been highly eutrophic in the past. Despite major reductions in external nutrient loading, including phosphorus, seasonal algal blooms in Deer Creek have not decreased to desired levels. Resuspension of sediment has been suggested as a potential source of internal nutrient loading for …

Nitrogen Mass Balance In A High Rate Algal Pond, Kevin Jones

Biomedical Engineering

The objective of the study described herein was to perform a mass balance on nitrogen in four small high rate algal ponds (HRAPs) in order to determine the fate of the nitrogen. The total percentage of nitrogen lost via ammonia volatilization was calculated via the mass balance.

Field Algae Measurements Using Empirical Correlations At Deer Creek Reservoir, Ryan A. Stephens

Theses and Dissertations

Deer Creek Reservoir in Utah has a history of high algae concentrations. Despite recent nutrient reduction efforts, seasonal algae continue to present problems. Cost effective, accurate, and comprehensive monitoring is important to understand the reservoir processes driving this problem and characterizing the algae spatial and temporal distributions are an important part of this effort. Current laboratory methods for accurately measuring algae are expensive and time consuming and are based on water samples taken in the field and transported to the laboratory. This approach only provides data for relatively few point samples because of the time and expense of sample collection …

Energy And Nutrient Recovery From Anaerobic Treatment Of Organic Wastes, Christian-Dominik Henrich

All Dissertations

The objective of the research was to develop a complete systems design and predictive model framework of a series of linked processes capable of providing treatment of landfill leachate while simultaneously recovering nutrients and bioenergy from the waste inputs. This proposed process includes an 'Ammonia Recovery Process' (ARP) consisting of: 1) ammonia de-sorption requiring leachate pH adjustment with lime or sodium hydroxide addition followed by, 2) ammonia re-absorption into a 6-molar sulfuric acid spray-tower followed by, 3) biological activated sludge treatment of soluble organic residuals (BOD) followed by, 4) high-rate algal post-treatment and finally, 5) an optional anaerobic digestion process …

Responses Of Problematic Algae To Algaecide Exposures, West Bishop

All Theses

Excessive growths of algae can cause significant disruption of critical water resource usages including: drinking, irrigation, recreation as well as be aesthetically displeasing. Water resource managers are often compelled to take action to mitigate these nuisance algal infestations. Algaecides can be efficient and effective management options to rapidly suppress algal blooms and restore water usages. However, laboratory and field data are required to accurately predict responses to an application as well as potential risks to non-target organisms. Laboratory experiments were used to measure the responses of problematic algae to algaecide exposures and evaluate the margin of safety to sensitive, non-target …

Extraction And Characterization Of Lipids From Microalgae Grown On Municipal Wastewater, Matthew W. Hutton

Master's Theses

Based on results of its Aquatic Species Program (1978-1996), which sought to develop algae-to-liquid fuel technology, the U.S. Department of Energy has suggested that algal wastewater treatment may be incorporated into biodiesel production schemes to reduce the operating costs of both processes. the purpose of the current research was to evaluate the triglycerides produced by wastewater-grown algae for their suitability as a fuel feedstock and to investigate the effectiveness of several solvent mixtures and extraction procedures at recovering lipids from fresh algae. The research involved two separate experiments. The first determined the quantity and quality of lipids produced over the …