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Articles 1 - 30 of 52
Full-Text Articles in Entire DC Network
A Limited Look At The Educational Experiences Of Black Stem Professionals From The Butler Community And Beyond, Victoria Ann Templin
A Limited Look At The Educational Experiences Of Black Stem Professionals From The Butler Community And Beyond, Victoria Ann Templin
Undergraduate Honors Thesis Collection
This project looks at the STEM educational experiences of Black professionals from both the Butler community and outside of it. It focuses on the perspectives of five individuals (three Butler graduates and two non-Butler graduates) interviewed via Zoom and analyzes how their experiences compare to those of other Black STEM professionals across the nation. After completing the interviews and conducting thorough background research, all data was examined. It was found that what was discussed during the interviews paralleled what was discovered in the research, such as the importance of mentors and broadening the image of what a scientist looks like. …
Investigation Of The Analytical Utility Of The Inductively Coupled Plasma Mass Spectrometer (Icp-Ms) For The Quantification Of Proteins Via Lanthanide Labeling, Caitlin Elizabeth Dunlap
Investigation Of The Analytical Utility Of The Inductively Coupled Plasma Mass Spectrometer (Icp-Ms) For The Quantification Of Proteins Via Lanthanide Labeling, Caitlin Elizabeth Dunlap
Undergraduate Honors Thesis Collection
The discovery and ability to provide an absolute concentration of biomarkers in the body can facilitate early diagnoses of diseases. This information can also be used for more accurate and unique treatments for those patients. While major advances in molecular mass spectrometry allows for a sensitive identification of these biomarkers, they are limited to only four orders of magnitude in their dynamic ranges. Due to this, detection and quantitation of low abundant proteins, which tend to make up these vital biomarkers, is very difficult. On the other hand, ICP-MS offers a wider dynamic range (up to 12 orders of magnitude) …
Nucleic Acid Editing By Adenosine Deaminase Enzymes, Josey Rae Mckinley
Nucleic Acid Editing By Adenosine Deaminase Enzymes, Josey Rae Mckinley
Undergraduate Honors Thesis Collection
RNA editing is crucial to the genetic diversity and structural complexity of organisms. ADAR (adenosine deaminase acting on RNA) is an RNA editing enzyme that creates a mutation of adenosine to inosine. The human ADAR2 (hADAR2) enzyme is known to edit RNA; however, it contains minor sequence homology with the cytidine deaminase enzymes, APOBEC and AID, which are known to edit both DNA and RNA (11 & 12). The ability of hADAR2 to edit DNA was tested through transformation into the Saccharomyces cerevisiae yeast strain BY4741. DNA editing was tested by determining if the transformed yeast became resistant to the …
Temporal Trends Of Persistent Organic Pollutants In Sarasota Bay Common Bottlenose Dolphins (Tursiops Truncatus), John Kucklick, Ashley Boggs, Kevin Huncik, Amanda Moors, Elizabeth Davis, Gina Ylitalo, Mary Mcconnell, Christina Makris, Randall S. Wells
Temporal Trends Of Persistent Organic Pollutants In Sarasota Bay Common Bottlenose Dolphins (Tursiops Truncatus), John Kucklick, Ashley Boggs, Kevin Huncik, Amanda Moors, Elizabeth Davis, Gina Ylitalo, Mary Mcconnell, Christina Makris, Randall S. Wells
Scholarship and Professional Work – COPHS
No abstract provided.
Structure-Guided Microbial Targeting Of Antistaphylococcal Prodrugs, Justin J. Miller, Ishaan T. Shah, Jayda Hatten, Yasaman Barekatain, Elizabeth A. Mueller, Ahmed M. Moustafa, Rachel L. Edwards, Cynthia S. Dowd, Geoffrey C. Hoops, R Jeremy Johnson
Structure-Guided Microbial Targeting Of Antistaphylococcal Prodrugs, Justin J. Miller, Ishaan T. Shah, Jayda Hatten, Yasaman Barekatain, Elizabeth A. Mueller, Ahmed M. Moustafa, Rachel L. Edwards, Cynthia S. Dowd, Geoffrey C. Hoops, R Jeremy Johnson
Scholarship and Professional Work - LAS
Carboxy ester prodrugs are widely employed to increase oral absorption and potency of phosphonate antibiotics. Prodrugging can mask problematic chemical features that prevent cellular uptake and may enable tissue-specific compound delivery. However, many carboxy ester promoieties are rapidly hydrolyzed by serum esterases, limiting their therapeutic potential. While carboxy ester-based prodrug targeting is feasible, it has seen limited use in microbes as microbial esterase-specific promoieties have not been described. Here we identify the bacterial esterases, GloB and FrmB, that activate carboxy ester prodrugs in Staphylococcus aureus. Additionally, we determine the substrate specificities for FrmB and GloB and demonstrate the structural basis …
Analytic Gradients For State-Averaged Multiconfiguration Pair-Density Functional Theory, Thais R. Scott, Matthew R. Hermes, Andrew M. Sand, Meagan S. Oakley, Donald G. Truhlar, Laura Gagliardi
Analytic Gradients For State-Averaged Multiconfiguration Pair-Density Functional Theory, Thais R. Scott, Matthew R. Hermes, Andrew M. Sand, Meagan S. Oakley, Donald G. Truhlar, Laura Gagliardi
Scholarship and Professional Work - LAS
Analytic gradients are important for efficient calculations of stationary points on potential energy surfaces, for interpreting spectroscopic observations, and for efficient direct dynamics simulations. For excited electronic states, as are involved in UV–Vis spectroscopy and photochemistry, analytic gradients are readily available and often affordable for calculations using a state-averaged complete active space self-consistent-field (SA-CASSCF) wave function. However, in most cases, a post-SA-CASSCF step is necessary for quantitative accuracy, and such calculations are often too expensive if carried out by perturbation theory or configuration interaction. In this work, we present the analytic gradients for multiconfiguration pair-density functional theory based on SA-CASSCF …
Comparative Analysis Of The Human Serine Hydrolase Ovca2 To The Model Serine Hydrolase Homolog Fsh1 From S. Cerevisiae, Jessica S. Bun, Michael D. Slack, Daniel E. Schemenauer, R. Jeremy Johnson
Comparative Analysis Of The Human Serine Hydrolase Ovca2 To The Model Serine Hydrolase Homolog Fsh1 From S. Cerevisiae, Jessica S. Bun, Michael D. Slack, Daniel E. Schemenauer, R. Jeremy Johnson
Scholarship and Professional Work - LAS
Over 100 metabolic serine hydrolases are present in humans with confirmed functions in metabolism, immune response, and neurotransmission. Among potentially clinically relevant but uncharacterized human serine hydrolases is OVCA2, a serine hydrolase that has been linked with a variety of cancer-related processes. Herein, we developed a heterologous expression system for OVCA2 and determined the comprehensive substrate specificity of OVCA2 against two ester substrate libraries. Based on this analysis, OVCA2 was confirmed as a serine hydrolase with a strong preference for long-chain alkyl ester substrates (>10-carbons) and high selectivity against a variety of short, branched, and substituted esters. Substitutional analysis …
The Characterization And Reactivity Of Tris(4-Methylpyrazolyl)Methane And Its Tungsten And Molybdenum Metal Complexes, Julia M. Pomeroy
The Characterization And Reactivity Of Tris(4-Methylpyrazolyl)Methane And Its Tungsten And Molybdenum Metal Complexes, Julia M. Pomeroy
Undergraduate Honors Thesis Collection
The scorpionate ligand tris(pyrazolyl)borate (Tp) has contributed to the understanding of coordination and organometallic chemistry and has proven to be useful in the synthesis of metalloprotein models. Studies focusing on the similar ligand, tris(pyrazolyl)methane (Tpm), have recently been made possible with improved synthetic techniques. Characterization of the Tpm ligand and its metal derivatives, [TpmW(CO)3L]+ and [TpmMo(CO)3L]+, unveiled unexpected activity in the 1H NMR chemical shift of the methane hydrogen after the coordination of a seventh ligand. The more electronegative the seventh ligand was, the more downfield the chemical shift appeared. Various analogs of Tpm have since been synthesized that substitute …
Seven Coordinate Molybdenum And Tungsten Complexes Containing Tpm And Tpm Derivatives And The Impact Of Ligand Substitution On Nmr Chemical Shifts, Cole Seager
Undergraduate Honors Thesis Collection
A series of known and new seven coordinate molybdenum and tungsten complexes of tris(pyrazolyl)methane (Tpm) and substituted Tpm, [TpmM(CO)3X]+, have been synthesized. Depending on the identity of X, (bromo, iodo, hydrido) and the substitution of the Tpm ligand, substantial chemical shift differences are observed for the hydrogen on the central carbon of the Tpm ligand. Factors impacting the chemical shift of the hydrogen on the central carbon of the Tpm ligand, such as the electron donating ability of the Tpm ligand and the electronegativity of the additional ligand on the metal, will be discussed.
Sub-Cloning, Expression, And Purification Of The Wild Type And Mutated Sigma-1 Receptor, Christopher Koch
Sub-Cloning, Expression, And Purification Of The Wild Type And Mutated Sigma-1 Receptor, Christopher Koch
Undergraduate Honors Thesis Collection
The Sigma-1 receptor (S1R) is an important pharmaceutical target that has been linked to several neurological diseases and drug addiction. It has been proposed that multimerization of S1R is important for attenuating its interactions with the dopamine transporter (DAT) and that S1R agonists promote dissociation to a monomeric form necessary for DAT interaction. This project aims to assess the role of the N-terminal transmembrane domain in oligomerization and binding activity of the mammalian Sigma-1 receptor (S1R). N-terminal truncations lacking the transmembrane domain and the E102Q variant, which displays altered activity and cellular localization, were expressed, purified in order to determine …
Case Studies Of Potential Reference Materials In Cultural Heritage Science, Heidi Kastenholz
Case Studies Of Potential Reference Materials In Cultural Heritage Science, Heidi Kastenholz
Undergraduate Honors Thesis Collection
The study of cultural heritage objects requires reference materials for comparison in order to determine the identity of chemical compounds in historically/culturally significant artifacts. The reference materials available today can be inaccurately labeled, can contain inauthentic compounds, or can simply be formed differently than what the labeled material should be. Utilizing the laboratory at the Indianapolis Museum of Art at Newfields, three case studies were conducted over three different potential reference materials in order to properly identify each of the three materials being focused on here. Based on the analysis, the labels for these materials often do not match the …
Using Ega-Gc To Analyze Nicotine N-Oxide In Order To Explain Low Nicotine Concentrations In E-Cigarette Liquids, Cory Wuerch
Using Ega-Gc To Analyze Nicotine N-Oxide In Order To Explain Low Nicotine Concentrations In E-Cigarette Liquids, Cory Wuerch
Undergraduate Honors Thesis Collection
Evolved-gas analysis coupled with gas chromatography (EGA-GC) was used to analyze e-cigarette liquids. Previous analyses of e-cigarette liquids have shown that the determined concentration of nicotine is lower than the advertised concentration. A possible explanation for this phenomenon is that the nicotine in the liquids is being oxidized to nicotine N-oxide by exposure to air and thus reducing the concentration of nicotine. This study focused on analyzing samples of thermally-rearranged nicotine N-oxide. Using EGA-GC, a calibration curve was generated for nicotine N-oxide concentration, which could potentially be used to explain reduced nicotine concentrations in e-cigarette liquids.
Producing Dihydrofurans Using Palladium (Ii) Catalyst And Optimized Base, Chandler Mitchell
Producing Dihydrofurans Using Palladium (Ii) Catalyst And Optimized Base, Chandler Mitchell
Undergraduate Honors Thesis Collection
Dihydrofurans serve as building blocks for other compounds in organic synthesis. The main goal of this project was to discover an efficient and relatively inexpensive pathway for producing monosubstituted dihydrofurans in high yield from cyclic boronic half acids. Aldehydes were converted to homoallylic alcohols by the addition of allylmagnesium bromide. The alcohols were then transformed into cyclic boronic half acids using ringclosing metathesis with Grubbs 1st Generation Catalyst and alkenyl boronic esters. Finally, monosubstituted dihydrofurans were produced using a palladium (II) catalyst with a base. Palladium (II) catalysts that were tested include [1,1'- bis(diphenylphosphino)ferrocene]palladium (II) dichloride, palladium (II) acetate with …
Quantitative Analysis Of Acetic Acid In Hard Woods As A Way To Improve Museum Artifact Storage, Alexandria Flora
Quantitative Analysis Of Acetic Acid In Hard Woods As A Way To Improve Museum Artifact Storage, Alexandria Flora
Undergraduate Honors Thesis Collection
Many materials used for storage of museum artifacts have been found to cause damage to the items either through emitting harmful volatile gases or through direct contact. Harmful storage techniques can cause corrosion, discoloration, or deterioration of priceless artifacts. Museum enclosures made of hardwoods have been shown to emit acetic acid vapors that cause damage to the items being stored. In the present study, a review of the effects of acetic acid damage on museum artifacts was performed, and an evolved gas analysis and titration method were used to quantify acetic acid in various hardwoods.
Spectroscopic Analysis Of The Traditional Cyanotype Process And Its Impact On Art, Jacob Applegarth
Spectroscopic Analysis Of The Traditional Cyanotype Process And Its Impact On Art, Jacob Applegarth
Undergraduate Honors Thesis Collection
Reflectance spectroscopy was used to study the cyanotype printing process. Originally this process was used to make photocopies of building designs, but today it is used for art. The cyanotype process is a photo catalyzed oxidation-reduction reaction. This study altered the concentrations and reagents involved in the printing process, and it included the use of ammonium ferricyanide and ammonium iron(III) oxalate along with the traditional potassium ferricyanide and ammonium iron(III) citrate. The impact of these changes on the rate of formation of Prussian blue was observed and quantified. Rate laws and general trends were determined for the reagents involved. Qualitative …
Use Of Trispyrazolemethane In The Synthesis Of Organometallic Compounds, Claire Hazelton
Use Of Trispyrazolemethane In The Synthesis Of Organometallic Compounds, Claire Hazelton
Undergraduate Honors Thesis Collection
Trispyrazoleborate is a negatively charged facially coordinating tridentate ligand that has been highly researched and used in many metal complexes. The related ligand trispyrazolemethane has not been widely studied due to its difficult synthesis. The objective of this project is to determine the effects of the neutral nature of the trispyrazolemethane on the metal carbonyl complexes of molybdenum and tungsten. Besides the ligand tricarbonyl complex, iodide and hydride derivatives were synthesized. All compounds were characterized with IR and HNMR.
The Unusual Substrate Specificity Of A Virulence Associated Serine Hydrolase From The Highly Toxic Bacterium, Francisella Tularensis, Alexander M. Farberg, Whitney K. Hart, R. Jeremy Johnson
The Unusual Substrate Specificity Of A Virulence Associated Serine Hydrolase From The Highly Toxic Bacterium, Francisella Tularensis, Alexander M. Farberg, Whitney K. Hart, R. Jeremy Johnson
Scholarship and Professional Work - LAS
Francisella tularensis is the causative agent of the highly, infectious disease, tularemia. Amongst the genes identified as essential to the virulence of F. tularensis was the proposed serine hydrolase FTT0941c. Herein, we purified FTT0941c to homogeneity and then characterized the folded stability, enzymatic activity, and substrate specificity of FTT0941c. Based on phylogenetic analysis, FTT0941c was classified within a divergent Francisella subbranch of the bacterial hormone sensitive lipase (HSL) superfamily, but with the conserved sequence motifs of a bacterial serine hydrolase. FTT0941c showed broad hydrolase activity against diverse libraries of ester substrates, including significant hydrolytic activity across alkyl ester substrates from …
Solvent Free Vanadium Mediated Organic Transformations, Garrett Oberst
Solvent Free Vanadium Mediated Organic Transformations, Garrett Oberst
Undergraduate Honors Thesis Collection
A mixture of vanadium(V) oxide and copper sulfate was used to catalyze oxidative transformations in a variety of organic compounds. Multiple functionalities were tested, including thiols, amines and alcohols. Various transformations were observed resulting in various oxidized products, including coupling reactions. Reactions were performed in a solvent-free environment due to the insoluble nature of vanadium(V) oxide in common organic solvents. Results were analyzed using HNMR and GCMS.
Chiroptical Ionic Liquids, Benjamin Zercher
Chiroptical Ionic Liquids, Benjamin Zercher
Undergraduate Honors Thesis Collection
The synthesis and fluorescent analysis of chiral light emitting ionic liquids made from lanthanide-doped chiral amino acid ionic liquids is described in this thesis. The ionic liquids were composed of tetrabutylammonium (TBA) cation with enantiomers of the amino acids valine, proline, and aspartate as anionic counterions. These ionic liquids of varying amino acid composition were doped with europium trifluoromethanesulfonate (triflate) and analyzed by circularly polarized luminescence (CPL). The sign of the CPL signal corresponded to the handedness of the amino acids in the ionic liquids. Europium triflate concentration dependent CPL in both [TBA][Val] and [TBA][L-Pro] ionic liquids was measured from …
Substrate Specificity Of The Lipn Hydrolase From Mycobacterium Ulcerans, Stephanie Raynor
Substrate Specificity Of The Lipn Hydrolase From Mycobacterium Ulcerans, Stephanie Raynor
Undergraduate Honors Thesis Collection
Mycobacterium ulcerans is the causative agent of Buruli ulcer, a tropical skin disease that affects thousands of individuals annually. Recent studies have revealed that lipolytic enzymes are involved in the pathogenicity processes of mycobacterium and could be potential targets for novel antibiotics. LipN is one proposed serine hydrolase in Mycobacterium ulcerans that contains the conserved α/β hydrolase protein fold and utilizes the conserved catalytic traid of serine, histidine, and aspartate/glutamate. The physiological substrate and biological role of LipN from M. ulcerans have not yet been determined. In this study, LipN was cloned into a pET28a plasmid and overexpressed in an …
Determination Of Additives In Cigarettes Utilizing Evolved Gas Analysis (Ega), Karissa Ferguson, Michael J. Samide, Cierra Schmicker, Tyler Schenk, Anne Wilson
Determination Of Additives In Cigarettes Utilizing Evolved Gas Analysis (Ega), Karissa Ferguson, Michael J. Samide, Cierra Schmicker, Tyler Schenk, Anne Wilson
Scholarship and Professional Work - LAS
While additives to consumer products must be listed in order by amount, it is not required to report the actual amounts of these additives. Given that impacts on human health are dose dependent, knowledge of additive amounts would aid discussions of the safety of these compounds for inhalation. Additives for cigarettes include flavors, such as menthol, and propylene glycol, a humectant. Rapid determination of the amounts of menthol and propylene glycol additives in cigarettes was accomplished utilizing evolved gas analysis (EGA) – gas chromatography (GC) – flame ionization detection (FID). Careful preparation of samples and appropriate sample storage are critical …
Synthesis And Resolution Of A Substituted Dioxolane From Glycerol, Susannah Dawn Cox
Synthesis And Resolution Of A Substituted Dioxolane From Glycerol, Susannah Dawn Cox
Undergraduate Honors Thesis Collection
Glycerol (Figure 1) is one of the major byproducts in the manufacturing of biofuels. As the interest in biodiesel grows, production of glycerol will also increase. Investigating glycerol's potential as a chemical feedstock could lead to the discovery of new uses for this byproduct created from biofuel production. By developing pathways to use glycerol as a starting material and as a solvent we will be able to synthesize other chemically relevant compounds.
Suzuki-Miyaura Reactions Are Used To Synthesize Z-Alkenes From Cyclic Boronic Half-Acids, Daniel Lee French
Suzuki-Miyaura Reactions Are Used To Synthesize Z-Alkenes From Cyclic Boronic Half-Acids, Daniel Lee French
Undergraduate Honors Thesis Collection
Cis-Alkenes provide the basic structure and reactivity for a variety of compounds, from the common chemicals found in any organic laboratory to advanced drugs used to treat cancer or suppress the immune system (Littke & Fu, 2002). Synthetically, alkenes are also of use in that they serve as a site for further chemistry. Historically, Z-alkenes have been more difficult to synthesize than their E-counterparts (McNulty et al., 2010; Siau, Zhang, & Zhao, 2012). Furthermore, in many reactions, a mixture of E- and Z- conformations of the alkene is produced. Such differences may seem trivial upon first inspection, but because there …
Amino Acid Methyl Ester Chiral Ionic Liquids: A Concentration Study, Christopher J. Brown
Amino Acid Methyl Ester Chiral Ionic Liquids: A Concentration Study, Christopher J. Brown
Undergraduate Honors Thesis Collection
This project will investigate novel amino acid methyl ester chiral ionic liquids and determine how increased concentration of lanthanide solute and changes in size of counter ions tetramethylammonium and tetrabutylammonium affects interaction between the ionic liquid and the fluorescent lanthanide solute. Both concentration dependence and temperature dependence were discovered. The chiral ionic liquid [L-prolineCl][TfiN] reacted uniquely to the change in concentration.
Thermal Denaturation Of Mycobacterium Tuberculosis Protein Rv0045c Monitored By Intrinsic Tryptophan Fluorescence, Lindsey Renee Drake
Thermal Denaturation Of Mycobacterium Tuberculosis Protein Rv0045c Monitored By Intrinsic Tryptophan Fluorescence, Lindsey Renee Drake
Undergraduate Honors Thesis Collection
The aim of my project was to use intrinsic tryptophan fluorescence to monitor the denaturation pathway of a protein, specifically the Mycobacterium tuberculosis enzyme Rv0045c. The larger scientific goal and inspiration for this honors thesis was to use the amino acid residues of a protein to map it's unfolding by thermal denaturation.
Combining Experiment And Theory To Elucidate The Role Of Supercritical Water In Sulfide Decomposition, Yuko Kida, Caleb A. Class, Anthony J. Concepcion, Michael T. Timko, William H Green
Combining Experiment And Theory To Elucidate The Role Of Supercritical Water In Sulfide Decomposition, Yuko Kida, Caleb A. Class, Anthony J. Concepcion, Michael T. Timko, William H Green
Scholarship and Professional Work – COPHS
The cleavage of C–S linkages plays a key role in fuel processing and organic geochemistry. Water is known to affect these processes, and several hypotheses have been proposed, but the mechanism has been elusive. Here we use both experiment and theory to demonstrate that supercritical water reacts with intermediates formed during alkyl sulfide decomposition. During hexyl sulfide decomposition in supercritical water, pentane and CO + CO2 were detected in addition to the expected six carbon products. A multi-step reaction sequence for hexyl sulfide reacting with supercritical water is proposed which explains the surprising products, and quantum chemical calculations provide quantitative …
Synthesis Of Fluorogenic Substrates For The Enzymatic Characterization Of Rv0045c From M. Tuberculosis, Kelly Jo Mckenna
Synthesis Of Fluorogenic Substrates For The Enzymatic Characterization Of Rv0045c From M. Tuberculosis, Kelly Jo Mckenna
Undergraduate Honors Thesis Collection
Mycobacterium tuberculosis is the pathogenic bacterial agent commonly responsible for tuberculosis, or TB. Although treatment exists for the active form of tuberculosis, no method has been developed for eliminating M tuberculosis in its dormant state. One hypothesized method for the elimination of dormant TB is to develop an inhibitor specific for M tuberculosis esterases and lipases, as these esterases and lipases are essential to the survival of dormant TB infection. In this research, the substrate specificity of the Rv0045c esterase from M tuberculosis was studied due to the essential role of Rv0045c in TB metabolism and its dissimilarity to other …
Chiral Recognition In Amino Acid And Peptide Based Chiral Ionic Liquids, Daniel Mccray Kroupa
Chiral Recognition In Amino Acid And Peptide Based Chiral Ionic Liquids, Daniel Mccray Kroupa
Undergraduate Honors Thesis Collection
In this study, amino acid and peptide based chiral ionic liquids (ILs) were prepared and their chiral recognition ability probed using circularly polarized luminescence (CPL) spectroscopy. Potential applications of amino acid and peptide based chiral ILs include asymmetric synthesis and chiral resolution. The amino acid based chiral ILs under study were prepared from amino acid methyl ester cations and bis(trifluoromethane)sulfonamide ([TF2ND anions, specifically L-alanine methyl ester, D-alanine methyl ester, L-Ieucine methyl ester, and L-proline methyl ester. Cationic peptide ILs were prepared from L-alanyl glycine methyl ester cations and [TF2Nr anions and anionic peptide based ILs were prepared from tetrabutylammonium ([TBAt) …
Characterization Of Esterase Activity From The Bacteria, Francisella Tularensis, The Causative Agent Of Tularemia, Leigh Anna Weston
Characterization Of Esterase Activity From The Bacteria, Francisella Tularensis, The Causative Agent Of Tularemia, Leigh Anna Weston
Undergraduate Honors Thesis Collection
Francisella tularensis is the bacteria responsible for causing the disease tularemia and is listed as one of the top three-biowarfare agents. Among the proteins essential to the virulence and infectivity of F.tularensis are multiple esterases, which are enzymes that break down various ester, thioester, and amide bonds. In this project, the catalytic activity, substrate speci fi city, and structure of a putative esterase from F.tularensis was studied. Latent fluorophores based on the molecule, fluorescein, were unmasked by the enzymatic activity of the esterase and the increase in fluorescence was measured over time to determine how well the e tcrase recognized …
Development Of A Green Method For Amide Synthesis, Megan Ruth Darragh
Development Of A Green Method For Amide Synthesis, Megan Ruth Darragh
Undergraduate Honors Thesis Collection
The aim of this work was to determine the scope and limitations of using microwave technology, solvent-free conditions and green chemistry-applicable solid supports for amide synthesis. We hypothesize that the method developed by Comerford et al [12] may be applicable to other solid support systems already in use (silica, fly ash, and alumina), and that combined with the use of microwave technology, green and efficient synthesis of varied amides and other dehydration reaction products may be possible. These methods should show application to undergraduate, organic chemistry laboratories as well as large-scale production laboratories.