Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Entire DC Network
Effects Of Keratin Filaments On Erk Signaling During Fas-Induced Death Of Cervical Cancer (Hela) Cells, Amanda Berger
Effects Of Keratin Filaments On Erk Signaling During Fas-Induced Death Of Cervical Cancer (Hela) Cells, Amanda Berger
Honors Theses and Capstones
Survival of cancer cells is influenced by a variety of factors, including physical elements such as keratin filaments. We know HeLa cells containing or lacking keratin 8/18 intermediate filaments (K+ and K- cells, respectively) are more sensitive to the death-inducing effects of Fas agonist compared to the cytokines tumor necrosis factor alpha (TNF-α) or TNF-related apoptosis-inducing ligand. Additionally, K- cells are more sensitive to Fas-induced death than K+ as shown by previous studies using mitochondrial activity and caspase activation assays. In the current study we tested the hypothesis that keratin filaments associate with the mitogen activated protein kinase (MAPK) cascade …
Defeating Cytoplasmic Sequestration Of P53 In Human Breast Cancer Cells; Is Mortalin Involved?, Sarah Yunes
Defeating Cytoplasmic Sequestration Of P53 In Human Breast Cancer Cells; Is Mortalin Involved?, Sarah Yunes
Honors Theses and Capstones
Cytoplasmic sequestration of p53, possibly caused by p53 interacting with mortalin, can prevent p53 from functioning in DNA repair and apoptosis, causing aberrant growth. This project treated SKBR3 breast cancer cells with MKT-077, a dye that is a competitive binder to mortalin to see if it would result in the release of p53 from the cytoplasm and restoration of p53 function. Treatment resulted in partial translocation of a protein suspected to be p53 to the nucleus and apoptosis initiated at the mitochondria.