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Articles 1 - 15 of 15
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Effects Of Keratin Filaments On Erk Signaling During Fas-Induced Death Of Cervical Cancer (Hela) Cells, Amanda Berger
Effects Of Keratin Filaments On Erk Signaling During Fas-Induced Death Of Cervical Cancer (Hela) Cells, Amanda Berger
Honors Theses and Capstones
Survival of cancer cells is influenced by a variety of factors, including physical elements such as keratin filaments. We know HeLa cells containing or lacking keratin 8/18 intermediate filaments (K+ and K- cells, respectively) are more sensitive to the death-inducing effects of Fas agonist compared to the cytokines tumor necrosis factor alpha (TNF-α) or TNF-related apoptosis-inducing ligand. Additionally, K- cells are more sensitive to Fas-induced death than K+ as shown by previous studies using mitochondrial activity and caspase activation assays. In the current study we tested the hypothesis that keratin filaments associate with the mitogen activated protein kinase (MAPK) cascade …
Investigating The Interactions Between Cyanobacteria And Vibrio Parahaemolyticus, Caroline E. Ward
Investigating The Interactions Between Cyanobacteria And Vibrio Parahaemolyticus, Caroline E. Ward
Honors Theses and Capstones
One well-known pathogen that has been the topic of many recent studies is Vibrio parahaemolyticus, which causes thousands of foodborne illnesses a year, mostly from the ingestion of raw or undercooked oysters. It has been shown cyanobacteria can act as a long-term reservoir of Vibrio cholerae, another pathogenic Vibrio, by encasing the cells within mucilaginous sheaths during which Vibrios enter a viable but non-culturable state. In this study we investigated the interaction of V. parahaemolyticus with cyanobacteria to determine whether cyanobacteria aid in the longevity and survival of V. parahaemolyticus. We found that non-pathogenic V. parahaemolyticus …
Diel Vertical Migration Strategies Of Zooplankton In Oligotrophic Russell Pond, New Hampshire, Kelsey Ann Dobe
Diel Vertical Migration Strategies Of Zooplankton In Oligotrophic Russell Pond, New Hampshire, Kelsey Ann Dobe
Honors Theses and Capstones
Russell Pond is an ultra-oligotrophic lake with low chlorophyll a (1.9 mg L-1), total phosphorus (3.4 mg L-1), high Secchi Disk (10.4 m) and high light transmission (water coefficient of water, kw=0.33). Vertical migration of Chaoborus, Bosmina, Daphnia, and copepods were examined using net collections of zooplankton discrete depth counts and sonar. Three contrasting patterns of vertical migration were observed in Russell Pond. Chaoborus punctipennis larvae vertical migration began at 7 pm and migrated through the entire lake water column, a total of 23 m from the sediments to the surface water in …
Will The Exploratory Behavior Of Lobsters Decrease As They Become Familiar With Their Environment?, Marissa Cuda
Will The Exploratory Behavior Of Lobsters Decrease As They Become Familiar With Their Environment?, Marissa Cuda
Honors Theses and Capstones
Previous studies have shown that most lobsters have a home range in which they reside on a daily basis. The tendency for lobsters to reside in a particular area suggests that they have the ability to learn the characteristics of an area using exploratory behavior. We hypothesize that the exploratory behavior of juvenile lobsters will decrease as time spent in a novel environment increases; specifically exploratory behavior will decrease as the lobsters continuously learn the environment. Exploratory activity of juvenile lobsters was monitored in six lobsters using two separate maze complexities. Lobsters were video recorded and activity was measured based …
The Effect Of Rider Level On Equine Mean Heart Rate At The Trot, Jacqueline Marinoff
The Effect Of Rider Level On Equine Mean Heart Rate At The Trot, Jacqueline Marinoff
Honors Theses and Capstones
The effect of riders at two different levels of riding ability on the mean heart rates (HR) of school horses at the trot was studied. Five horses were each paired with a beginner (n=4) and an intermediate (n=5) rider, and the mean HR generated by the two riders at the trot were compared for each horse. There was a significant (p
The Possible Role Of Extra-Ocular Photoreceptors In The Entrainment Of Lobster Circadian Rhythms To Light:Dark Cycles, Sara A. Mckinney
The Possible Role Of Extra-Ocular Photoreceptors In The Entrainment Of Lobster Circadian Rhythms To Light:Dark Cycles, Sara A. Mckinney
Honors Theses and Capstones
The American lobster, Homarus americanus, is a decapod crustacean whose daily nocturnal rhythms of activity are driven, in part, by an internal biological clock. While this circadian clock is capable of producing a rhythm of approximately 24 hours on its own, it can also be entrained to light:dark cycles. Recent findings in our laboratory suggest that lobsters have photosensitive neurons throughout their nervous system (extra-ocular photoreceptors). The objective of this research was to test the hypothesis that these extra-ocular photoreceptors aid in entraining their daily rhythm of activity. First, the locomotion of juvenile American lobsters was measured in trials under …
Filtering Efficiency And Feeding Mechanisms Of Daphnia Pulex On Microcystis Aeruginosa And Nannochloropsis, Elizabeth Orlowicz
Filtering Efficiency And Feeding Mechanisms Of Daphnia Pulex On Microcystis Aeruginosa And Nannochloropsis, Elizabeth Orlowicz
Honors Theses and Capstones
The filtering and feeding rate of Daphnia pulex from the Old Durham Reservoir, Durham, NH, were measured to determine the feeding efficiency on different concentrations of non-toxic Nannochloropsis (Class Eustigmatophyceae) and toxigenic cyanobacteria Microcystis aeruginosa. Direct observations of thoracic appendage beats and post-abdominal rejections were also measured at 5*105 and 106 cells mL-1 concentrations of Nannochloropsis and M. aeruginosa to examine the feeding mechanisms of the D. pulex. In the presence of increasing Nannochloropsis concentrations, the filtering rates decreased and the feeding rates increased. When exposed to M. aeruginosa, both the filtering and feeding …
Effects Of Incremental Dietary Levels Of Ground Flaxseed On Milk Production, Ruminal Metabolism, And Enteric Methane Emissions In Organic Dairy Cows, Katie Velez
Honors Theses and Capstones
Ground Flaxseed (Linum uitatissimum) is a lipid supplement that is commonly fed to dairy cows. It is believed that supplemental lipid can change the Fatty Acid (FA) composition in the milk, and decrease methane production. Twenty lactating organic Jersey cows, housed at the UNH Organic Dairy Research Farm (ODRF), were randomly assigned to five replicated 4 × 4 Latin squares to investigate the effects of increasing dietary levels of ground flaxseed (0, 5, 10, or 15% of the diet dry matter) on animal fperformance (e.g., dry matter intake, milk production, milk composition), ruminal metabolism, and enteric methane emissions. Each …
Minireview: Protein Interactions, Jessica Child
Minireview: Protein Interactions, Jessica Child
Honors Theses and Capstones
No abstract provided.
One Can’T Stand On Its Own: Are Non-Luminescence Traits Necessary For V. Fischeri Colonization Of E. Scolopes?, Feier Liu
Honors Theses and Capstones
Vibrio fischeri and Euprymna scolopes squid establish mutualistic symbiosis and select for each other in the natural environment. V. fischeri provides bioluminescent camouflage for E. scolopes while E. scolopes provides nutrients for V. fischeri. The most intriguing aspect of this relationship is that E. scolopes is highly selective and only allows sustained colonization by luminous, but not dark V. fischeri. Luminescence is the key symbiosis trait; however, other bacterial factors may also allow squid recognition. We hypothesized that there are luminescence linked traits that contribute to colonization. V. fischeri with luminescence variation was isolated and tested for oxidative resistance, …
The Effects Of Cytokinin On The Transcriptional Regulation Of Pin Expression In Arabidopsis Thaliana, Elizabeth Burgess
The Effects Of Cytokinin On The Transcriptional Regulation Of Pin Expression In Arabidopsis Thaliana, Elizabeth Burgess
Honors Theses and Capstones
The processes of cell division and differentiation are critical to the development of any multicellular organism. During the formation of plant roots these processes take place at a region of the root tip called the meristem. Cytokinin and auxin are two plant growth hormones that influence this process. Although these two growth hormones are both necessary they also appear in many ways to have an antagonistic relationship. As meristematic root cells undergo differentiation they cease dividing. It has been proposed that the size of the root meristem and thus the overall rate of root growth are determined by the balance …
Genetic Engineering Of Dunaliella: Potential For Improved Biofuel Production, Alison Wirshing
Genetic Engineering Of Dunaliella: Potential For Improved Biofuel Production, Alison Wirshing
Honors Theses and Capstones
Biodiesel produced from algal lipids is a promising source of renewable energy. Reasons for using algae, as opposed to vascular plants, are numerous. Oleaginous algae do not require fertile land or fresh water to grow, and therefore do not compete with food crop resources. Algae have short generation times and higher growth rates than larger vascular plants allowing for synthesis and accumulation of large quantities of neutral lipids, about 20-50% of the dry cell weight, in a short period of time. The efficiency of biodiesel production from oleaginous algae could be improved by using transgenic (genetically engineered) algae. The goal …
Transformation Of Tobacco With The Ba5 Cement Protein Gene From Balanus Amphitrite, Matt Marquis
Transformation Of Tobacco With The Ba5 Cement Protein Gene From Balanus Amphitrite, Matt Marquis
Honors Theses and Capstones
Expressing barnacle cement proteins genes such as the BA5 gene in plants may enable individual study and analysis. This technique is effective since barnacle cement is difficult to work with as a whole in the lab setting. The BA5 gene extracted from Balanus amphitrite is transferred to tobacco leaf tissue using Agrobacterium tumefaciens.
Defeating Cytoplasmic Sequestration Of P53 In Human Breast Cancer Cells; Is Mortalin Involved?, Sarah Yunes
Defeating Cytoplasmic Sequestration Of P53 In Human Breast Cancer Cells; Is Mortalin Involved?, Sarah Yunes
Honors Theses and Capstones
Cytoplasmic sequestration of p53, possibly caused by p53 interacting with mortalin, can prevent p53 from functioning in DNA repair and apoptosis, causing aberrant growth. This project treated SKBR3 breast cancer cells with MKT-077, a dye that is a competitive binder to mortalin to see if it would result in the release of p53 from the cytoplasm and restoration of p53 function. Treatment resulted in partial translocation of a protein suspected to be p53 to the nucleus and apoptosis initiated at the mitochondria.
The Effects Of Dependent Infants On The Social Behavior Of Mantled Howler Monkeys (Alouatta Palliate), Keziah Katz
The Effects Of Dependent Infants On The Social Behavior Of Mantled Howler Monkeys (Alouatta Palliate), Keziah Katz
Honors Theses and Capstones
Six weeks of observation on eight groups of wild mantled howler monkeys revealed that the presence of a dependent infant alters the frequency of social interactions between a female with an infant and other adult monkeys. Males preferred to spend time with females without infants but still spent more time with females with infants than with males. Females without infants spent significantly less time with females with infants than with males or other females without infants and females with infants decreased their frequency of social interaction overall without preference for males, females or other females with infants.