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Localization Of The Follicle-Stimulating Hormone Receptor In Lipid Raft Domains, Tyler Esposito

Honors Theses

Lipid raft domains have been shown to be important in receptor signaling, especially for G Protein Coupled Receptors (GPCR). Follicle stimulating hormone is a vital reproductive signal that relies on effective signaling of its GPCR. The goal of this study was to test whether or not the follicle stimulating hormone receptor (FSHR) utilizes lipid raft domains when signaling. The main methods used were fluorescent antibodies and various forms of microscopy, including confocal microscopy. A fluorescent antibody for the FSHR (mAb 106.105) allowed us to perform these studies. In the first experiment, cells treated with FSH and stained with the receptor …