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Isolation Of A Dosage Dependent Suppressor Using Saccharomyces Cerevisiae As A Model System, Rachel Chimner
Isolation Of A Dosage Dependent Suppressor Using Saccharomyces Cerevisiae As A Model System, Rachel Chimner
Masters Theses
Human pathogenic Yersinia use a type three secretion system to deliver various effector proteins into host cells. Once these effector proteins are within the cell, they elicit a cascade of events that disrupt the normal immune response. One of these effectors, YopT, is known to disrupt actin distribution but it is currently unknown what YopT targets within the host cell. To investigate the cellular targets of the YopT effector, we use a yeast model system and a dosage-dependent suppression screen. The dosage-dependent suppression screen isolated three plasmids able to suppress YopT induced lethality within yeast. One of them, 2T9, was …