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Mitochondrial Dysfunction And Reactive Oxygen Species In Neurodegeneration, Leah Farina
Mitochondrial Dysfunction And Reactive Oxygen Species In Neurodegeneration, Leah Farina
Honors Theses
Neurodegeneration leads to the loss of normal anatomy and physiology of the neuronal system in a human body. The hallmark of most neurodegenerative diseases is abnormal folding and accumulation of proteins within neuronal cell bodies that is not present in healthy people. These changes in normal protein metabolism often lead to neuronal cell death and failure of the affected regions of the central nervous system. Abnormal protein accumulation may be attributed to dysfunctional mitochondria and damage by reactive oxygen species. In addition to causing altered protein accumulation, dysfunctional mitochondria and an excess of reactive oxygen species also lead to errors …
Mechanisms Of Neuroprotection Against Retinal Ganglion Cell Loss Using An In Vivo Glaucoma Model, Kevin Heinze
Mechanisms Of Neuroprotection Against Retinal Ganglion Cell Loss Using An In Vivo Glaucoma Model, Kevin Heinze
Honors Theses
Glaucoma is an incurable ocular disease characterized by apoptotic cell death and degeneration of retinal ganglion cells (RGCs), and is one of the leading causes of blindness worldwide (Quigley and Broman, 2006; Quigley et al., 1995). While current treatments are effective at slowing vision loss, glaucoma’s multifactorial etiology has made it difficult for researchers to identify a target for treatment that will permanently halt RGC degeneration. Previous studies have proposed a glutamate-induced excitotoxic mechanism of RGC death in glaucoma, and neuroprotective agents have been investigated to prevent this effect. One agent, acetylcholine, has been shown to initiate pro-survival signaling cascades …
Population Demographics And Genetics Of Spix's Disk-Winged Bat: Insights Regarding Survival, Mate Choice, Gene Flow And Effective Population Size, Michael Buchalski
Population Demographics And Genetics Of Spix's Disk-Winged Bat: Insights Regarding Survival, Mate Choice, Gene Flow And Effective Population Size, Michael Buchalski
Dissertations
Simultaneous study of the demographics and genetics of populations are relatively rare within the literature, despite insights their combined use offers regarding the life history, ecology, and evolution of species. Here I take a comprehensive approach, using capture-recapture data, polymorphic microsatellite markers, and various modeling techniques to examine the demographics and genetics of multiple local populations of Spix’s disk-winged bat (Thyroptera tricolor) in southwestern Costa Rica. T. tricolor is a highly gregarious, neotropical bat species known to form kin-based social groups with high retention of offspring of both sexes. The implications of this highly unusual social structure for …
Use Of A Specific Alpha7 Nicotinic Acetylcholine Receptor Agonist Can Prevent Loss Of Retinal Ganglion Cells In An In Vivo Rat Glaucoma Model, David Mata
Masters Theses
Acetylcholine (ACh) has been shown to have a neuroprotective effect against glutamate-induced excitotoxicity in cultured retinal ganglion cells (RGCs) through activation of α7 nicotinic ACh receptors (nAChRs). In this study, the neuroprotective effects of the α7 nAChR agonist, PNU-282987, were investigated using an in vivo model of glaucoma in adult Long Evans rats. Hypertonic saline injections were used to induce a glaucoma model. After one month, retinas were removed, flat mounted, fixed and labeled with an antibody against Thy 1.1 to label RGCs and fluorescently tagged for visualization. Eye drop application of PNU-282987 resulted in neuroprotection against RGC loss in …
Discovery And Characterization Of A Tanapoxvirus Encoded Human Neuregulin Mimetic, David Jeng
Discovery And Characterization Of A Tanapoxvirus Encoded Human Neuregulin Mimetic, David Jeng
Dissertations
Studies on large dsDNA viruses such as poxviruses have been helpful in identifying a number of viral and cellular growth factors that contribute to our broad understanding of virus-host interaction. Orthopoxviruses and leporipoxviruses are among the most studied viruses in this aspect. However, tanapoxvirus (TPV) a member of the genus Yatapoxvirus, still remains largely unexplored, as the only known hosts for this virus are humans and monkeys. Here, we describe the initial characterization of an EGF-like growth factor mimicking human neuregulin from TPV, expressed by the TPV-15L gene. Assays using a baculovirus expressed and tagged TPV-15L protein demonstrated the ability …
Isolation Of A Dosage Dependent Suppressor Using Saccharomyces Cerevisiae As A Model System, Rachel Chimner
Isolation Of A Dosage Dependent Suppressor Using Saccharomyces Cerevisiae As A Model System, Rachel Chimner
Masters Theses
Human pathogenic Yersinia use a type three secretion system to deliver various effector proteins into host cells. Once these effector proteins are within the cell, they elicit a cascade of events that disrupt the normal immune response. One of these effectors, YopT, is known to disrupt actin distribution but it is currently unknown what YopT targets within the host cell. To investigate the cellular targets of the YopT effector, we use a yeast model system and a dosage-dependent suppression screen. The dosage-dependent suppression screen isolated three plasmids able to suppress YopT induced lethality within yeast. One of them, 2T9, was …