Digital Commons Network^™

Large Animal Genetic Engineering Summit 2020, Irina A. Polejaeva

Funded Research Records

No abstract provided.

Understanding Free-Roaming Horse Conflicts With Wildlife In The Great Basin, S. Nicole Frey, Eric Thacker, Katlyn Uhart

All Current Publications

Horses and burros were introduced to North America in the 1600s with European missionaries and explorers. Over time, abandoned and released horses and burros formed herds and by the time European-American settlers began to explore North America in the 1700s, these free-roaming horses and burros had adapted to their habitat and been incorporated into Native American culture. By the mid-1900s, most Americans considered these horses as wild and symbols of freedom and beauty.

In 1971, Congress passed the Wild and Free-Roaming Horses and Burros Act (Public Law 73-482) to provide federal protection for free-roaming horses and burros (also known as …

Exploration Of Pregnancy Recognition In Ruminants Using A Novel Type I Interferon Receptor Knockout Sheep Model, Christopher J. Davies

Funded Research Records

No abstract provided.

Nad Metabolism In Male Reproductive Aging, Mirella L. Meyer-Ficca

Funded Research Records

No abstract provided.

Identifying Fox Squirrels And Their Damage In Your Yard, S. Nicole Frey, Jessica Tegt

All Current Publications

Native to the eastern United States, fox squirrels (Sciurus niger) were first documented in Utah in 2011. It is unknown how fox squirrels made their way into Utah; however, it is possible they followed riparian corridors (areas along rivers, where trees often grow) through Colorado into Utah and were only detected once their populations grew in number. Since the Utah Natural Heritage Program began tracking them, there have been over 500 observations of this species, predominantly around Salt Lake City and the Wasatch Front. Fox squirrels are the largest tree squirrel in the U.S. (17–28 inches, and 17.5–37.5 ounces). This …

Memantine Treatment Reduces The Incidence Of Flaccid Paralysis In A Zika Virus Mouse Model Of Temporary Paralysis With Similarities To Guillain-Barré Syndrome, Venkatraman Siddharthan, Hong Wang, Alexandre Lr De Oliveria, Xin Dai, John D. Morrey

Animal, Dairy, and Veterinary Science Faculty Publications

Clinical evidence suggests that Zika virus contributes to Guillain-Barré syndrome that causes temporary paralysis. We utilized a recently described Zika virus mouse model of temporary flaccid paralysis to address the hypothesis that treatment with an N-methyl-D-aspartate receptor antagonist, memantine, can reduce the incidence of paralysis. Aged interferon alpha/beta-receptor knockout mice were used because of their sublethal susceptibility to Zika virus infection. Fifteen to twenty-five percent of mice infected with a Puerto Rico strain of Zika virus develop acute flaccid paralysis beginning at days 8–9 and peaked at days 10–12. Mice recover from paralysis within a week of onset. In …

Association Between Single Nucleotide Polymorphisms In Sirt1 And Sirt2 Loci And Growth In Tibetan Sheep, Lin-Sheng Gui, Sayed Haidar Abbas Raza, Li Zhou, Matthew Garcia, Ayman Hassan Abd El-Aziz, Dawei Wei, Shengzhen Hou, Jianlei Jia, Zhiyou Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Silent information regulator 1 and 2 (SIRT1, 2) were NAD+-dependent histone or non-histone deacetylase, which emerged as key metabolic sensors in several tissues of mammals. In the present study, the search for polymorphisms within the ovine SIRT1 and SIRT2 loci as well as association analyses between SNPs and growth-related traits were performed in Tibetan sheep. To determine the expression pattern of SIRT1 and SIRT2 genes in Tibetan sheep, the quantitative real-time polymerase chain reaction (qPCR) analysis revealed that those two genes were widely expressed in diverse tissues. Expression of SIRT1 was less in abomasum of lamb, whereas it …

Mammals Of Cedar Breaks National Monument, S. Nicole Frey, Natalie D'Souza, Ethan Hammer, Bryan Larsen, Jake Nielson

All Current Publications

This document is the result of a study of the terrestrial mammals found at Cedar Breaks National Monument from 2017-2019. During this time, we conducted a survey of mammals using a series of trail cameras. We used survey results to provide an index of terrestrial mammals (excluding bats) within the park. Our goal is to provide a beginner’s guide to the mammals at Cedar Breaks National Monument. We include information regarding appearance, diet, habitat, and population status according to the International Union for the Conservation of Nature (IUCN). The guide represents the species a visitor to Cedar Breaks National Monument …

Characterization Of A Novel Stat 2 Knock Out Hamster Model Of Crimean Congo Hemorrhagic Fever Virus Pathogenesis, Charlene Ranadheera, Emelissa J. Valcourt, Bryce M. Warner, Guillaume Poliquin, Kyle Rosenke, Kathy Frost, Kevin Tierney, Greg Saturday, Jinxin Miao, Jonna B. Westover, Brian B. Gowen, Stephanie Booth, Heinz Feldmann, Zhongde Wang, David Safronetz

Animal, Dairy, and Veterinary Science Faculty Publications

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne pathogen causing a febrile illness in humans, which can progress to hemorrhagic manifestations, multi-organ failure, and death. Current mouse models of CCHFV infection reliably succumb to virus challenge but vary in their ability to reflect signs of disease similar to humans. In this study, we established a signal transducer and activator of transcription 2 (STAT2) knockout hamster model to expand the repertoire of animal models of CCHFV pathogenesis that can be used for therapeutic development. These hamsters demonstrated a systemic and lethal disease in response to infection. Hallmarks of human …

What Drives Bull Prices, And How Much Can I Spend On A Bull?, Matthew D. Garcia, Ryan Larsen, C. K. Chapman

All Current Publications

Several factors determine the age-old question of how much to pay for a bull. These factors are highly variable and can fluctuate from year to year. The five main factors influencing bull prices are explored in this fact sheet, including breed, availability, auction activity, breeder reputation, and genetic potential.

Raman Spectroscopy Characterization Extracellular Vesicles From Bovine Placenta And Peripheral Blood Mononuclear Cells, Han Zhang, Ana Caroline Silva, Wei Zhang, Heloisa M. Rutigliano, Anhong Zhou

Animal, Dairy, and Veterinary Science Faculty Publications

Placenta-derived extracellular vesicles (EVs) are involved in communication between the placenta and maternal immune cells possibly leading to a modulation of maternal T-cell signaling components. The ability to identify EVs in maternal blood may lead to the development of diagnostic and treatment tools for pregnancy complications. The objective of this work was to differentiate EVs from bovine placenta (trophoblast) and peripheral blood mononuclear cells (PBMC) by a label-free, non-invasive Raman spectroscopy technique. Extracellular vesicles were isolated by ultracentrifugation. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) were applied to verify the presence and the size distribution of EVs. Raman …

Equine Dental Care, Karl Hoopes, Patricia Evans, Diana Witkopf

All Current Publications

Just as people visit the dentist on a regular basis to maintain proper dental health, horses also require regular dental care. Horses should have at least annual dental check-ups to maintain proper alignment and remove points and hooks. This publication provides facts and suggestions on basic equine dental care for horses of all ages.

Equine Nutrition: Concentrates, Karl Hoopes, Patricia Evans, Scott Mckendrick

All Current Publications

Concentrate (grain) should be considered only as supplements to good quality hay. In general, a mature horse does not require the energy provided by concentrate-type feeds (grains/sweet feeds, pelleted feed, etc.) unless the horse is used for more than light work or in production, such as a lactating mare or a breeding stallion. Too many times horses are overfed rather than underfed. This fact sheet will help horse owners understand the digestive process and the general feeding requirements, including different grains and mixed grains, for their horses.

Comparison Of In Vitro Inactivation Of Sars Cov-2 With Hydrogen Peroxide And Povidone-Iodine Oral Antiseptic Rinses, Avinash S. Bidra, Jesse S. Pelletier, Jonna B. Westover, Samantha Frank, Seth M. Brown, Belachew Tessema

Animal, Dairy, and Veterinary Science Faculty Publications

Purpose

To evaluate the in vitro inactivation of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) with hydrogen peroxide (H2O2) and povidone‐iodine (PVP‐I) oral antiseptic rinses at clinically recommended concentrations and contact times.

Materials and Methods

SARS‐CoV‐2, USA‐WA1/2020 strain virus stock was prepared prior to testing by growing in Vero 76 cells. The culture media for prepared virus stock was minimum essential medium (MEM) with 2% fetal bovine serum (FBS) and 50 µg/mL gentamicin. Test compounds consisting of PVP‐I oral rinse solutions and H2O2 aqueous solutions were mixed directly with the virus solution so that the final concentration was 50% of …

Utah Land Management Evaluation And Assessment Network Needs Assessment, Jordan Smith

All Current Publications

It can be difficult to make and implement land management decisions that are informed by the best available science, satisfy different stakeholders, and are compliant with established policies and regulations. Local, state, and federal entities with the capacity to influence land management decisions, can benefit from an understanding of the shared needs of landowners and managers. This needs assessment reports on the shared information, funding, and research needs expressed by land managers and owners throughout Utah who make decisions about: (1) fire prevention and suppression; (2) livestock grazing; (3) fisheries; or (4) wildlife. The report provides a high-level assessment of …

Rapid In-Vitro Inactivation Of Severe Acute Respiratory Syndrome Coronavirus 2 (Sars-Cov-2) Using Povidone-Iodine Oral Antiseptic Rinse, Avinash S. Bidra, Jesse S. Pelletier, Jonna B. Westover, Samantha Frank, Seth M. Brown, Belachew Tessema

Animal, Dairy, and Veterinary Science Faculty Publications

Purpose

To investigate the optimal contact time and concentration for viricidal activity of oral preparation of povidone‐iodine (PVP‐I) against SARS‐CoV‐2 (‘corona virus’) to mitigate the risk and transmission of the virus in the dental practice.

Materials and Methods

The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) USA‐WA1/2020 strain, virus stock was tested against oral antiseptic solutions consisting of aqueous povidone‐iodine (PVP‐I) as the sole active ingredient. The PVP‐I was tested at diluted concentrations of 0.5%, 1%, and 1.5%. Test media without any virus was added to 2 tubes of the compounds to serve as toxicity and neutralization controls. Ethanol (70%) …

Aging Horses By Their Teeth, Karl Hoopes, Patricia Evans, Nancy Jack

All Current Publications

This publication tells how to pinpoint a horses age, within a fairly narrow range, by examining its teeth. Being able to pinpoint age can be of use to owners of unregistered horses or horses whose age is unknown for any reason. Many health and nutrition management decisions are directly related to age and dental wear, making it even more important for all horse owners to have a general understanding of how a horse’s mouth changes with age. From a historical perspective, up until recently when organized associations started keeping birth dates on registered horses, most horse professionals took great pride …

Type I Interferon Underlies Severe Disease Associated With Junín Virus Infection In Mice, Brady T. Hickerson, Eric J. Sefing, Kevin W. Bailey, Arnaud J. Van Wettere, Manuel L. Penichet, Brian B. Gowen

Animal, Dairy, and Veterinary Science Student Research

Junín virus (JUNV) is one of five New World mammarenaviruses (NWMs) that causes fatal hemorrhagic disease in humans and is the etiological agent of Argentine hemorrhagic fever (AHF). The pathogenesis underlying AHF is poorly understood; however, a prolonged, elevated interferon-α (IFN-α) response is associated with a negative disease outcome. A feature of all NWMs that cause viral hemorrhagic fever is the use of human transferrin receptor 1 (hTfR1) for cellular entry. Here, we show that mice expressing hTfR1 develop a lethal disease course marked by an increase in serum IFN-α concentration when challenged with JUNV. Further, we provide evidence that …

Development, Characterization, And Application Of Two Reporter-Expressing Recombinant Zika Viruses, Sang-Im Yun, Byung-Hak Song, Michael E. Woolley, Jordan C. Frank, Justin G. Julander, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV), a mosquito-borne transplacentally transmissible flavivirus, is an enveloped virus with an ~10.8 kb plus-strand RNA genome that can cause neurological disease. To facilitate the identification of potential antivirals, we developed two reporter-expressing ZIKVs, each capable of expressing an enhanced green fluorescent protein or an improved luminescent NanoLuc luciferase. First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. Two reporter-expressing ZIKVs were then generated by …

Influence Of Total Western Diet On Docosahexaenoic Acid Suppression Of Silica-Triggered Lupus Flaring In Nzbwf1 Mice, Kristen N. Gilley, Kathryn A. Wierenga, Preeti S. Chauhaun, James G. Wagner, Ryan P. Lewandowski, Elizbeth A. Ross, A. L. Lock, Jack R. Harkema, Abby D. Benninghoff, James J. Pestka

Animal, Dairy, and Veterinary Science Faculty Publications

Lupus is a debilitating multi-organ autoimmune disease clinically typified by periods of flare and remission. Exposing lupus-prone female NZBWF1 mice to crystalline silica (cSiO₂), a known human autoimmune trigger, mimics flaring by inducing interferon-related gene (IRG) expression, inflammation, ectopic lymphoid structure (ELS) development, and autoantibody production in the lung that collectively accelerate glomerulonephritis. cSiO₂-triggered flaring in this model can be prevented by supplementing mouse diet with the ω-3 polyunsaturated fatty acid (PUFA) docosahexaenoic acid (DHA). A limitation of previous studies was the use of purified diet that, although optimized for rodent health, does not reflect the …

Evolutionary Selection Against Short Nucleotide Sequences In Viruses And Their Related Hosts, Yoram Zarai, Zohar Zafrir, Bunpote Siridechadilok, Amporn Suphatrakul, Modi Roopin, Justin G. Julander, Tamir Tuller

Animal, Dairy, and Veterinary Science Faculty Publications

Viruses are under constant evolutionary pressure to effectively interact with the host intracellular factors, while evading its immune system. Understanding how viruses co-evolve with their hosts is a fundamental topic in molecular evolution and may also aid in developing novel viral based applications such as vaccines, oncologic therapies, and anti-bacterial treatments. Here, based on a novel statistical framework and a large-scale genomic analysis of 2,625 viruses from all classes infecting 439 host organisms from all kingdoms of life, we identify short nucleotide sequences that are under-represented in the coding regions of viruses and their hosts. These sequences cannot be explained …

Pasture Management To Improve Dry Matter Intake, Marcus F. Rose, Earl Creech, Blair L. Waldron, S. Clay Isom, Michael Peel, Kara Thornton-Kurth, Jacob Hadfield, Kerry A. Rood

All Current Publications

Agricultural producers are constantly looking for ways to maximize returns while reducing input costs. On dairy operations, a move from confinement feeding to pasture grazing offers the potential to reduce costs associated with harvest and storage of feed. In such a transition, producers sometimes report a decline in milk production and growth of livestock—both of which can strongly correlate to dry matter intake. Fortunately, dry matter intake is something that can be influenced by management practices. In this publication, we discuss the pasture management practices to improve dry matter intake.

Size And Scope Of Carbon County Agriculture 2019, Ryan Feuz, Ryan Larsen, Steven Price

All Current Publications

Carbon County is located south of Duchesne and Wasatch counties in east-central Utah. Along with Emery County to the south, it is often referred to as Utah’s Castle Country. The county became industrialized very early after statehood with the arrival of the railroads and discovery of many coal beds for which the county is named. It is also a large producer of natural gas within the state. Its primary economic base today comes from regional services, retail, tourism, recreation and resource extraction. As of 2017, the population was estimated at 20,295. Price is the largest city and county seat. This …

Utah's Own Habitat Guidelines For Sage-Grouse, David K. Dahlgren, Michel Kohl, Eric Thacker, S. Nicole Frey, Terry Messmer

All Current Publications

Those living and working within western North America’s sagebrush landscapes have most likely been exposed to sage-grouse issues for many years now and may have felt anxiety concerning the potential impacts that could result from implementing conservation measures designed to help this iconic species persist. Society’s challenge to seek the delicate balance between the conservation of nature and the growth related to human endeavors exemplifies the need to use the best available science as we move forward. Representative information, especially at the landscape level related to sage-grouse, has been hard to come by. However, due to the vast amount of …

Theoretical Risk Of Genetic Reassortment Should Not Impede Development Of Live, Attenuated Rift Valley Fever (Rvf) Vaccines Commentary On The Draft Who Rvf Target Product Profile, Thomas P. Monath, Jeroen Kortekaas, Douglas M. Watts, Rebecca C. Christofferson, Angelle Desiree Labeaud, Brian B. Gowen, Clarence J. Peters, Darci R. Smith, Robert Swanepoel, John C. Morrill, Thomas G. Ksiazek, Phillip R. Pittman, Brian H. Bird, George Bettinger

Animal, Dairy, and Veterinary Science Faculty Publications

In November 2019, The World Health Organization (WHO) issued a draft set of Target Product Profiles (TPPs) describing optimal and minimally acceptable targets for vaccines against Rift Valley fever (RVF), a Phlebovirus with a three segmented genome, in both humans and ruminants. The TPPs contained rigid requirements to protect against genomic reassortment of live, attenuated vaccines (LAVs) with wild-type RVF virus (RVFV), which place undue constraints on development and regulatory approval of LAVs. We review the current LAVs in use and in development, and conclude that there is no evidence that reassortment between LAVs and wild-type RVFV has occurred during …

Understanding The Influence Of Trenbolone Acetate And Polyamines On Proliferation Of Bovine Satellite Cells, Caleb C. Reichhardt, Amir Ahmadpour, Rachael G. Christensen, Nikole E. Ineck, Gordon Murdoch, Kara Thornton-Kurth

Animal, Dairy, and Veterinary Science Student Research

Approximately 90% of beef cattle on feed in the United States receive at least one anabolic implant, which results in increased growth, efficiency, and economic return to producers. However, the complete molecular mechanism through which anabolic implants function to improve skeletal muscle growth remains unknown. This study had 2 objectives: (1) determine the effect of polyamines and their precursors on proliferation rate in bovine satellite cells (BSC); and (2) understand whether trenbolone acetate (TBA), a testosterone analog, has an impact on the polyamine biosynthetic pathway. To address these, BSC were isolated from 3 finished steers and cultured. Once cultures reached …

Uncovering The Relationship Between Dietary Fatty Acids And Fiber Digestion In The Rumen, Fernanda Batistel

Funded Research Records

No abstract provided.

Predictors Of Puma Occupancy Indicate Prey Vulnerability Is More Important Than Prey Availability In A Highly Fragmented Landscape, Courtney A. C. Coon, Peter J. Mahoney, Emilie Edelblutte, Zara Mcdonald, David C. Stoner

Wildland Resources Faculty Publications

Habitat fragmentation represents the single greatest conservation challenge of the 21st century. This problem is particularly acute for large, obligate carnivores like pumas Puma concolor which have persisted in North and South America in the face of habitat fragmentation and other anthropogenic disturbances. Shrinking habitat and reduced connectivity mean that mapping habitat is increasingly important for species conservation in multiple-use landscapes. Previous work suggests that pumas occupy habitats where sufficient stalking cover and preferred prey are present, yet the intersection of these factors has rarely been assessed. Here we used data from 68 299 camera trap nights collected from 181 …

The First Record Of Raccoon Dog (Nyctereutes Procyonoides) In Turkey, Morteza Naderi, Emrah Çoban, Josip Kusak, Mübeccel Çisel Kemahli Aytekin, Mark William Chynoweth, İsmail Kayahan Ağirkaya, Neslihan Güven, Ayşegül Çoban, Çağan Hakkı Şekercioğlu

Wildland Resources Faculty Publications

The raccoon dog (Nyctereutes procyonoides) was recorded for the first time in Turkey on May 10, 2019, and June 5, 2019, in the same location after 4668 nights of camera trapping in the forests of the Sarıkamış region and Allahuekber Mountains in eastern Turkey. It was recorded in a Scots pine (Pinus sylvestris) forest at 2340 m above sea level with extensive snow cover. Given that the nearest known population is in the forests of Georgia approximately 105 km away, there may already be a viable population in Turkey. As an omnivorous species with a high …

Consumption Of The Total Western Diet Promotes Colitis And Inflammation-Associated Colorectal Cancer In Mice, Abby D. Benninghoff, Korry J. Hintze, Stephany P. Monsanto, Daphne Michelle Rodriguez Jimenez, Ashli H. Hunter, Sumira Phatak, James J. Pestka, Arnaud J. Van Wettere, Robert E. Ward

Animal, Dairy, and Veterinary Science Faculty Publications

Consumption of a Western type diet is a known risk factor for colorectal cancer. Our group previously developed the total Western diet (TWD) for rodents with energy and nutrient profiles that emulate a typical Western diet. In this study, we tested the hypothesis that consumption of the TWD would enhance colitis, delay recovery from gut injury and promote colon tumorigenesis. In multiple experiments using the azoxymethane + dextran sodium sulfate or Apc^Min/+ mouse models of colitis-associated colorectal carcinogenesis (CAC), we determined that mice fed TWD experienced more severe and more prolonged colitis compared to their counterparts fed the standard …