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Agriculture

Clemson University

Tissue culture

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Development Of In Vitro Protocols To Enhance Secondary Metabolite Production From Turmeric (Curcuma Longa L.), Matthew Cousins May 2008

Development Of In Vitro Protocols To Enhance Secondary Metabolite Production From Turmeric (Curcuma Longa L.), Matthew Cousins

All Theses

Plant and organ culture can readily be applied to the field of medicinal plant and secondary metabolite production. Many studies that have been conducted attest to the utility of in vitro methods for preservation of genetic diversity, propagation of clonal individuals for use in commercial field planting, and production of metabolites directly from culture. Experiments have also shown that tissue culture is an important tool for enhancement of medicinal compound production utilizing various methods including elicitation. This thesis focuses on production of metabolites using an in vitro system. An overview in the form of a literature review is provided, which …


In Vitro Liquid Culture Systems Of Scutellaria Species, Ayse Tascan May 2007

In Vitro Liquid Culture Systems Of Scutellaria Species, Ayse Tascan

All Theses

In vitro culture of three Scutellaria species (S. lateriflora, S. costaricana and S. baicalensis) compared growth on different culture systems; agar and liquid cultures; stationary (LS), agitated (LA) and floating paper (FP). Fresh (FW), dry (DW), % dry weight (%DW=DW/FW), multiplication ratio (MR) and, sucrose, media and water use were compared. LA and LS plantlets were more hyperhydric, had more biomass, except S. baicalensis in LS, and less MR compared to agar and FP plantlets. A second experiment was conducted comparing liquid, agar and polyester fiber matrixes at different initial media volumes-30 mL and 20 mL. Plantlets from fiber had …