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The Effects Of Zinc Oxide Nanoparticles Loaded With Iaa And Iba On In Vitro Rooting Ofapple Microcuttings, Shabnam Alizadeh, Hati̇ce Dumanoğlu

Turkish Journal of Agriculture and Forestry

In the micropropagation of difficult-to-root plants, the low rates of rooting cause high economic losses. The local apple (Malus domestica Borkh.) cultivar #67, which is highly resistant to fire blight disease, has been shown to have low rooting success. Nanoparticles loaded with auxins could increase the rooting success with their physicochemical properties. To increase rooting percentage, the effects of zinc oxide nanoparticles (nZnO), nZnO loaded with indole-3-acetic acid (IAA-nZnO) and indole-3-butyric acid (IBA-nZnO) at different concentrations (1.0, 2.0, 3.0, 4.0, 5.0, and 6.0 mg L-1) on in vitro rooting of microcuttings were investigated. The concentrations of auxin carried by the …

Screening Of Factors Affecting Somatic Callusing And Embryo Induction In Allium Cepa L.Through Plackett?Burman Methodology, Kourosh Keighobadi, Maryam Golabadi, Mahdi Khozaei, Abdol Majid Rezai

Turkish Journal of Agriculture and Forestry

Induction of embryonic callus through available asexual explant culture is applied in the protection and maintenance of cytoplasmic-male sterility and in the maintenance of other lines of onion (Allium cepa L.). Usage of the Plackett?Burman (PB) experimental design has been suggested as a screening method for many variables that influence the desired induction. In order to overcome limitations of standard experimental designs and prioritization of media culture components, a systematic investigation was carried out using the PB design. In this investigation, the effects of different factors such as 2,4-D, putrescine, carbohydrate sources (maltose, sorbitol, and sucrose), silver nitrate, glutamine, and …

Exploration Of The Uae Native Plants For Sustainable Landscaping In Arid Region, Mubarak Mohammed S. R. Jaber Al Khaili

Philosophy Dissertations

In order to maintain sustainability for landscapes in the arid region, the use of native plants is of considerable importance, in the light of the fact that the exotic plants currently used do not resource efficient. The native plants adapted to the harsh environment of the desert ecosystem could play a critical role in this direction where the natural resources could be sustainably used. Research on evaluation and analyzes of native plant species of the UAE for potential application in landscapes have been considered. The present investigation reports the results of the exploration of native plants suited for sustainable landscape …

Improvement Of The In Vitro Regeneration And Agrobacterium-Mediated Genetic Transformation Of Medicago Sativa L., Farzad Nofouzi, Muhammet Çağri Oğuz, Saber Delpasand Khabbazi, Ali̇ Ergül

Turkish Journal of Agriculture and Forestry

Alfalfa is a fodder crop that accounts for one of the best sources of protein and is widely cultivated around the world. In vitro regeneration of alfalfa has been studied earlier; however, most of the studies were almost intervened with callus formation. In this study, 3 explant sources (cotyledonary node, hypocotyl, and root crown) of two Turkish cultivars (Nimet and Savaş) were excised from young seedlings. Explants were subjected to different concentrations of BAP, BAP-IBA, and TDZ to evaluate the direct in vitro regeneration potential of selected plant parts. Moreover, we transformed the alfalfa plant with pBin19 harboring 35s.GUS-INT_35s.nptII construct …

Evaluation Of Hall’S Panicgrass (Panicum Hallii Vasey) As A Model System For Genetic Modification Of Recalcitrance In Switchgrass (Panicum Virgatum (L.)), Joshua Nathaniel Grant

Masters Theses

While switchgrass (Panicum virgatum (L.)) has long been recognized as a viable bioenergy feedstock, it and other plants have cell walls with recalcitrance to processing. Recalcitrance is recognized as a major barrier to broad adoption of switchgrass and other feedstocks for cellulosic bioenergy. In an effort to reduce recalcitrance, transgenic plants have been generated with altered cell wall phenotypes such as reduced lignin. Unfortunately, stable transformation of switchgrass and other C4 grasses is time intensive, costly, and genetic analysis is further complicated by polyploid genomic structures. Unlike switchgrass, which can be tetraploid to octoploid, a closely related species, Hall’s …

Genotypic-Unspecific Protocols For The Commercial Micropropagation Of Eucalyptus Grandis × Nitens And E. Grandis × Urophylla, Maria Paula Watt

Turkish Journal of Agriculture and Forestry

Clones of Eucalyptus grandis × nitens and E. grandis × urophylla were subjected to various culture conditions at every culture stage leading to plantlet regeneration directly from axillary buds. The objective was to determine the possibility of using the same protocol for all clones. Although genotypic effects were evident, generally most clones responded similarly to the tested variations in each of the protocol stages, i.e. bud break, multiplication, and rooting. Estimated yields for the clones of one of the tested E. grandis × nitens hybrids ranged from 24 to 90 acclimatised plants per explant, but those of a natural hybrid …

In Vitro Screening Of Octoploid Fragaria Chiloensis And Fragaria Virginiana Genotypes Against Iron Deficiency, Ayfer Alkan Torun, Yildiz Kaçar, Belgi̇n Bi̇çen, Nazi̇fe Erdem, Sedat Serçe

Turkish Journal of Agriculture and Forestry

Iron (Fe) deficiency is one of the common problems in strawberry (Fragaria × ananassa Duch.) growing regions. In vitro screening enables researchers to evaluate genotype performance under several concentrations of Fe in a relatively fast and economical way. Therefore, we evaluated the responses of several genotypes belonging to octoploid Fragaria chiloensis (L.) Mill. and Fragaria virginiana Mill. (the progenitors of cultivated strawberry) against Fe treatments under in vitro conditions. In the first experiment, HM 1, Pigeon Point, CFRA1267 (F. chiloensis subsp. pacifica), and CA1541 (F. chiloensis subsp. chiloensis) were tested with various Fe concentrations (0%, 10%, 15%, 25%, 50%, 65%, …

Regeneration And Histological Analysis Of Snake Melon (Cucumis Melo Var. Flexuosus (L.) Naudin) By Direct Organogenesis, Yeşi̇m Yalçin Mendi̇, Selay Eldoğan, Rafael Gutakev, Muzaffer İpek, Pembe Çürük, Seli̇m Çeti̇ner

Turkish Journal of Agriculture and Forestry

In vitro morphogenesis of Cucumis melo var. flexuosus (L.) Naudin was studied by direct organogenesis. Induction of adventitious buds was obtained from distal and proximal parts of the cotyledon incubated on Murashige and Skoog (1962) medium containing different concentrations of 6-benzyladenine (BA) (0.0, 0.5, 1.0, and 2.0 mg L^{-1}) and indole-3-acetic acid (IAA) (0.0, 0.1, and 0.5 mg L^{-1}). The best organogenesis was obtained from the proximal part of the cotyledon on medium containing 0.5 mg L^{-1} BA and 0.5 mg L^{-1} IAA (88%). This concentration was used for histological analysis. Less regeneration was obtained on media containing 1.0 mg …

Development Of In Vitro Protocols To Enhance Secondary Metabolite Production From Turmeric (Curcuma Longa L.), Matthew Cousins

All Theses

Plant and organ culture can readily be applied to the field of medicinal plant and secondary metabolite production. Many studies that have been conducted attest to the utility of in vitro methods for preservation of genetic diversity, propagation of clonal individuals for use in commercial field planting, and production of metabolites directly from culture. Experiments have also shown that tissue culture is an important tool for enhancement of medicinal compound production utilizing various methods including elicitation. This thesis focuses on production of metabolites using an in vitro system. An overview in the form of a literature review is provided, which …

In Vitro Liquid Culture Systems Of Scutellaria Species, Ayse Tascan

All Theses

In vitro culture of three Scutellaria species (S. lateriflora, S. costaricana and S. baicalensis) compared growth on different culture systems; agar and liquid cultures; stationary (LS), agitated (LA) and floating paper (FP). Fresh (FW), dry (DW), % dry weight (%DW=DW/FW), multiplication ratio (MR) and, sucrose, media and water use were compared. LA and LS plantlets were more hyperhydric, had more biomass, except S. baicalensis in LS, and less MR compared to agar and FP plantlets. A second experiment was conducted comparing liquid, agar and polyester fiber matrixes at different initial media volumes-30 mL and 20 mL. Plantlets from fiber had …

In Vitro Propagation Of Some New Banana Types (Musa Spp.), Hami̇de Gübbük, Mustafa Pekmezci̇

Turkish Journal of Agriculture and Forestry

Three newly selected superior banana types were used to study the effects of different cytokinin and auxins on shoot multiplication and rooting. Benzylaminopurine [(BAP) (5, 10, 20 and 30 µM)] and thidiazuron [(TDZ) (0.4, 1, 2 and 3 µM)] were tested alone and with 1 µM indoleacetic acid (IAA) for the propagation stage. We compared basal Murashige and Skoog (MS) medium, active charcoal (5 g l^{-1}), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) for rooting. Shoot proliferation and elongation were significantly greater with TDZ than with BAP all 3 types. Furthermore, each cytokinin with IAA increased shoot proliferation and elongation …

In Vitro Plantlet Regeneration From Mature Embryos Of Linden (Tilia Platyphyllos Scop.) And Multiplication Of Its Buds, Ali̇ Ömer Üçler, Nuray Mollamehmetoğlu

Turkish Journal of Agriculture and Forestry

In this study, the effects of different sucrose, BAP and Kinetin concentrations in MS medium on plantlet formation, bud formation on the plantlets and development of embryos isolated from the seeds of Tilia platyphyllos under in vitro conditions were investigated. In addition, the effects of different culture times on the development of embryos and plantlet formation were studied. The results indicated that the cultured embryos on the media supplemented with sucrose (10, 20, 30, 40, 50 and 60 g/l) developed plantlets with epicotyls and roots. The best results were obtained on the medium supplemented with 30 g/l of sucrose. Epicotyls …

Multiplication Of Four Penstemon Species In Vitro, Dale T. Lindgren, Brent Mccown

West Central Research and Extension Center, North Platte

Penstemon (beard tongue) is a native genus of U.S. wildflower that is used for landscape plants (Lindgren, 1984a, 1984b, 1990), for cut flowers (Lindgren, 1986), and for ecological studies (Stubbendieck et al., 1982). Tissue culture techniques could be useful for propagating cultivars and species in this genus as some do not breed true from seed, require special seed germination conditions, or are difficult to propagate using other vegetative methods (Lindgren, 1984b, 1990; Stubbendieck et al., 1982). Penstemon haydenii is also the only listed endangered plant species in Nebraska.

The Penstemon spp. P. digitalis Nutt. ‘Husker Red’ (Lindgren, 1984b); P. grandiflorus …