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United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
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Comparison Of Three Alternative Ann Designs For Monthly Rainfall-Runoff Simulation, Jurgen D. Garbrecht
Comparison Of Three Alternative Ann Designs For Monthly Rainfall-Runoff Simulation, Jurgen D. Garbrecht
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The performance of three artificial neural network (ANN) designs that account differently for the effects of seasonal rainfall and runoff variations were investigated for monthly rainfall-runoff simulation on an 815 km2 watershed in central Oklahoma. The ANN design that accounted explicitly for seasonal variations of rainfall and runoff performed best by all performance measures. Explicit representation of seasonal variations was achieved by use of a separate ANN for each calendar month. For the three ANN designs tested, a regression of simulated versus measured runoff displayed a slope slightly under 1 and positive intercept, pointing to a tendency of the …
Evaluating The Influence Of Storage Time, Sample-Handling Method, And Filter Paper On The Measurement Of Water-Extractable Phosphorus In Animal Manures, Kristen Baum, Gary Pierzynski, Peter Kleinman, John Kovar, Rory Maguire, Philip Moore, Tiequan Zhang
Evaluating The Influence Of Storage Time, Sample-Handling Method, And Filter Paper On The Measurement Of Water-Extractable Phosphorus In Animal Manures, Kristen Baum, Gary Pierzynski, Peter Kleinman, John Kovar, Rory Maguire, Philip Moore, Tiequan Zhang
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Surface-applied manures create a potential phosphorus (P) runoff hazard, especially when unincorporated. In such cases, the concentration of water- extractable P in the manure has been correlated to soluble P concentrations in runoff. This study evaluated the influence of holding time, sample-handling procedure, and filtration method on measurement of the water-extractable P content of manures in a 3 ×3 ×2 factorial arrangement of treatments. A two-way interaction between holding time and sample-handling procedure occurred for most samples. Six samples had waterextractable P concentrations that were less than or equal to dried and dried/ground treatments. Only one sample had higher water-extractable …
Natural Abundance Of Foliar 15N As An Early Indicator Of Nitrogen Deficiency In Fertilized Cotton, S. Stamatiadis, C. Christofides, C. Tsadilas, V. Samaras, James S. Schepers
Natural Abundance Of Foliar 15N As An Early Indicator Of Nitrogen Deficiency In Fertilized Cotton, S. Stamatiadis, C. Christofides, C. Tsadilas, V. Samaras, James S. Schepers
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Information on the contribution of various soil nitrogen (N) sources to plant N uptake is often needed for the implementation of sustainable or site-specific management practices in agriculture. Considering the limitations of traditional methods in meeting these needs, this study investigated the potential of leaf δ15N as an early indicator of nutrient deficiency in cotton. The spatial and temporal natural abundance of 15N was measured in the soil and leaves of a fertilized cotton field located near the village of Moschochori (Larissa, Greece). The isotopic signal of the leaves was interpreted in the context of the relative …
Spermatogenesis In The Turkey (Meleagris Gallopavo): Quantitative Approach In Immature And Adult Males Subjected To Various Photoperiods, Jérôme Noirault, Jean-Pierre Brillard
Spermatogenesis In The Turkey (Meleagris Gallopavo): Quantitative Approach In Immature And Adult Males Subjected To Various Photoperiods, Jérôme Noirault, Jean-Pierre Brillard
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The objectives of this study were to identify and quantitate the germ cell populations of the testes in sexually mature male turkeys (Trial 1), determine the duration of meiosis based on BrdU labeling and stereological analyses (Trial 2), and examine the impact of various photoperiods on germinal and somatic cell populations in immature and adult males (Trial 3). In Trial 1, both testes within a male had similar stereological components (P > 0.05) for all parameters analyzed. In Trial 2, the duration of Type-1 spermatocytes and round spermatids in turkeys lasted 4.5 ± 0.5 and 2.0 ± 0.5 days, respectively. In …
Characterization Of Storage Proteins In Wild (Glycine Soja) And Cultivated (Glycine Max) Soybean Seeds Using Proteomic Analysis, Savithiry S. Natarajan, Chenping Xu, Hanhong Bae, Thomas J. Caperna, Wesley M. Garrett
Characterization Of Storage Proteins In Wild (Glycine Soja) And Cultivated (Glycine Max) Soybean Seeds Using Proteomic Analysis, Savithiry S. Natarajan, Chenping Xu, Hanhong Bae, Thomas J. Caperna, Wesley M. Garrett
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
A combined proteomic approach was applied for the separation, identification, and comparison of two major storage proteins, β-conglycinin and glycinin, in wild (Glycine soja) and cultivated (Glycine max) soybean seeds. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) with three different immobilized pH gradient (IPG) strips was an effective method to separate a large number of abundant and less-abundant storage proteins. Most of the subunits of β-conglycinin were well separated in the pH range 3.0-10.0, while acidic and basic glycinin polypeptides were well-separated in pH ranges 4.0-7.0 and 6.0-11.0, respectively. Although the overall distribution pattern of the protein spots …
Estrogenic Activity In The Environment: Municipal Wastewater Effluent, River, Ponds, And Wetlands, Nancy W. Shappell
Estrogenic Activity In The Environment: Municipal Wastewater Effluent, River, Ponds, And Wetlands, Nancy W. Shappell
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Estrogenic activity of regional water samples was evaluated. Samples obtained from wetlands and ponds involved in various agricultural land uses, from three river sites over four seasons, and from municipal wastewater effluent held in storage lagoons were evaluated. The estrogen- responsive cell line MCF-7 BOS was used in the E-screen assay to determine 17β-estradiol equivalents (E2 Eq) of water samples extracted by solid-phase extraction. Estrogenic activity in surrounding wetlands and ponds from different land uses was not different, with 10-12 M E2 Eq (0.3 ppt). Estrogenic activity of Red River samples was within the same range as wetland–pond …
Net Global Warming Potential And Greenhouse Gas Intensity In Irrigated Cropping Systems In Northeastern Colorado, Arvin R. Mosier, Ardell D. Halvorson, Curtis A. Reule, Xuejun J. Liu
Net Global Warming Potential And Greenhouse Gas Intensity In Irrigated Cropping Systems In Northeastern Colorado, Arvin R. Mosier, Ardell D. Halvorson, Curtis A. Reule, Xuejun J. Liu
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The impact of management on global warming potential (GWP), crop production, and greenhouse gas intensity (GHGI) in irrigated agriculture is not well documented. A no-till (NT) cropping systems study initiated in 1999 to evaluate soil organic carbon (SOC) sequestration potential in irrigated agriculture was used in this study to make trace gas flux measurements for 3 yr to facilitate a complete greenhouse gas accounting of GWP and GHGI. Fluxes of CO2, CH4, and N2O were measured using static, vented chambers, one to three times per week, year round, from April 2002 through October 2004 …
Microarray Based Comparison Of Two Escherichia Coli O157:H7 Lineages, Scot E. Dowd, Hiroshi Ishizaki
Microarray Based Comparison Of Two Escherichia Coli O157:H7 Lineages, Scot E. Dowd, Hiroshi Ishizaki
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Background: Previous research has identified the potential for the existence of two separate lineages of Escherichia coli O157:H7. Clinical isolates tended to cluster primarily within one of these two lineages. To determine if there are virulence related genes differentially expressed between the two lineages we chose to utilize microarray technology to perform an initial screening.
Results: Using a 610 gene microarray, designed against the E. coli O157 EDL 933 transcriptome, targeting primarily virulence systems, we chose 3 representative Lineage I isolates (LI groups mostly clinical isolates) and 3 representative Lineage II isolates (LII groups mostly bovine isolates). Using standard dye …
Post-Weaning Performance Of Steers From Varying Calving And Weaning Strategies In Montana, E. E. Grings, W. A. Phillips, R. E. Short, H. Mayeux, R. K. Heitschmidt
Post-Weaning Performance Of Steers From Varying Calving And Weaning Strategies In Montana, E. E. Grings, W. A. Phillips, R. E. Short, H. Mayeux, R. K. Heitschmidt
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The impact of varied calving and weaning times on post-weaning production of steer calves was evaluated in a 3- yr study. Steers (approximately 12 steers per calving-weaning treatment per year) born in late winter or early spring were weaned at 190 or 240 d of age, and steers born in late spring were weaned at 140 or 190 d of age after grazing with their dams on native range. Steers were pen-fed a growing diet until approximately 375 kg BW. They were then moved to an individual feeding facility and fed a higher-energy diet. Steers were allotted to harvest dates …
Traps And Trapping Techniques For Adult Mosquito Control, Daniel L. Kline
Traps And Trapping Techniques For Adult Mosquito Control, Daniel L. Kline
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
An overview is presented of the recent advancements in research activities conducted to evaluate mosquito traps. insecticide-impregnated targets baited with combinations of attractants, and strategies for using mass trapping techniques for adult mosquito population management. Technologies that use semiochemicals (attractants). traps and targets. and mass trapping are relatively new for management of adult mosquito populations. To date. emphasis has been placed primarily on developing barriers of attractant-baited and insecticide-impregnated targets. The most successful continuous use of this type of technology has been at Stevens' Landing. Collier County. Florida, Recently. commercially available traps have been evaluated for their ability to reduce …
Suppression Of House Flies (Diptera: Muscidae) In Florida Poultry Houses By Sustained Releases Of Muscidifurax Raptorellus And Spalangia Cameroni (Hymenoptera: Pteromalidae), Christopher Geden, Jerome Hogsette
Suppression Of House Flies (Diptera: Muscidae) In Florida Poultry Houses By Sustained Releases Of Muscidifurax Raptorellus And Spalangia Cameroni (Hymenoptera: Pteromalidae), Christopher Geden, Jerome Hogsette
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Weekly releases of Muscidifurax raptorellus Kogan and Legner and Spalangia cameroni Perkins were made for 12 wk after house cleanouts in Florida pullet houses in either spring/summer (May-August) or fall (September-December). Releases were made by weekly placement of 62,500 and 85,000 pupae parasitized by M. raptorellus and S. cameroni, respectively, which produced an average of 79,049 and 32,841 adult female parasitoids per week. House fly (Musca domestica L.) pupal mortality, as measured by sentinel pupae, was about twice as high in the release house (40.2%) as in the two control houses (21.5 and 21.8%) in the summer release. …
Prevention Of Mosquitoes (Diptera: Culicidae) And House Flies (Diptera: Muscidae) From Entering Simulated Aircraft With Commercial Air Curtain Units, David A. Carlson, Jerome Hogsette, Daniel Kline, Chris D. Geden, Robert Vandermeer
Prevention Of Mosquitoes (Diptera: Culicidae) And House Flies (Diptera: Muscidae) From Entering Simulated Aircraft With Commercial Air Curtain Units, David A. Carlson, Jerome Hogsette, Daniel Kline, Chris D. Geden, Robert Vandermeer
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Commercially available air curtain units were used to create air barriers to prevent mosquitoes and house flies from entering a simulated aircraft doorway together with passengers. Two assemblies of simulated passenger bridge and aircraft were constructed, and airflow measurements were recorded to confirm airflow characteristics for several combinations of commercial units. Three mosquito species were selected for different host-seeking characteristics, and house flies were selected to represent a large, strong-flying insect. Batches of 20 or 200 insects of four species were released into the passenger bridge just before 25 persons passed through the assembly, then insects that entered the aircraft …
Genetic And Hybridization Evidence Confirms That A Geographic Population Of Gonatocerus Morrilli (Hymenoptera: Mymaridae) From California Is A New Species: Egg Parasitoids Of The Glassy-Winged Sharpshooter Homalodisca Coagulata (Homoptera: Cicadellidae), Jesse H. De León, Walker A. Jones, Mamoudou Sétamou, David J. W. Morgan
Genetic And Hybridization Evidence Confirms That A Geographic Population Of Gonatocerus Morrilli (Hymenoptera: Mymaridae) From California Is A New Species: Egg Parasitoids Of The Glassy-Winged Sharpshooter Homalodisca Coagulata (Homoptera: Cicadellidae), Jesse H. De León, Walker A. Jones, Mamoudou Sétamou, David J. W. Morgan
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
We investigated the differentiation and reproductive isolation among different geographic populations of Gonatocerus morrilli, egg parasitoids of the glassy-winged sharpshooter (Homalodisca coagulata), to confirm previous observations that there may exist a cryptic species complex or a new species. Two mitochondrial genes [cytochrome oxidase subunits I (COI) and II (COII)] and the internal transcribed spacer region 2 (ITS2) of several individuals per population were sequenced. G. morrilli populations from Texas (TX), Florida (FL), California (CA), and an outgroup (G. ashmeadi) were analyzed. For comparison, a population from Argentina (G. annulicornis) morphologically similar to G. …
Biotinylation Of K8 And K12 Co-Occurs With Acetylation And Mono-Methylation In Human Histone H4, Yap Ching Chew, Ashraf S. Raza, Gautam Sarath, Janos Zempleni
Biotinylation Of K8 And K12 Co-Occurs With Acetylation And Mono-Methylation In Human Histone H4, Yap Ching Chew, Ashraf S. Raza, Gautam Sarath, Janos Zempleni
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Histones H1, H2A, H2B, H3, and H4 are proteins that are critical for folding of DNA into chromatin. Posttranslational acetylation, methylation, and biotinylation of histones participate in gene silencing, mitotic condensation of chromatin, and the cellular response to DNA damage. Various modifications of histones are known to interact ("cross-talk") in chromatin-remodeling events; interactions may be synergistic or antagonistic. Here, we sought to identify biotinylation sites in human histone H4 by using mass spectrometry (MS/MS), and we sought to determine whether biotinylation co-exists with acetylation and methylation in the same H4 molecule. Nuclear histones from human lymphoid (Jurkat) cells were digested …
Slow Ligand Binding Kinetics Dominate Ferrous Hexacoordinate Hemoglobin Reactivities And Reveal Differences Between Plants And Other Species, Benoit J. Smagghe, Gautam Sarath, Emily Ross, Jean-Louis Hilbert, Mark S. Hargrove
Slow Ligand Binding Kinetics Dominate Ferrous Hexacoordinate Hemoglobin Reactivities And Reveal Differences Between Plants And Other Species, Benoit J. Smagghe, Gautam Sarath, Emily Ross, Jean-Louis Hilbert, Mark S. Hargrove
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Hexacoordinate hemoglobins are found in many living organisms ranging from prokaryotes to plants and animals. They are named “hexacoordinate” because of reversible coordination of the heme iron by a histidine side chain located in the heme pocket. This endogenous coordination competes with exogenous ligand binding and causes multiphasic relaxation time courses following rapid mixing or flash photolysis experiments. Previous rapid mixing studies have assumed a steady-state relationship between hexacoordination and exogenous ligand binding that does not correlate with observed time courses for binding. Here, we demonstrate that this assumption is not valid for some hexacoordinate hemoglobins, and that multiphasic time …
Nutrient Uptake Of Maize Affected By Nitrogen And Potassium Fertility In A Humid Subtropical Environment, H. Arnold Bruns, M. Wayne Ebelhar
Nutrient Uptake Of Maize Affected By Nitrogen And Potassium Fertility In A Humid Subtropical Environment, H. Arnold Bruns, M. Wayne Ebelhar
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Nitrogen (N) and potassium (K) fertility management of maize (Zea mays L.) in the humid subtropical Mississippi Delta may differ from a temperate climate because of its use in rotation with cotton (Gossypium hirsutum L.), soil temperatures rarely falling to 0 °C, and heavy winter rains that facilitate nutrient losses. An experiment to determine the [N] (concentration = [ ]), phosphorus [P], [K], calcium [Ca], magnesium [Mg], iron [Fe], manganese [Mn], zinc [Zn], and copper [Cu] and their total contents plant-1 of maize grown in rotation with cotton, using N fertility levels of (134, 179, 224, 269, …