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Studies Of Chemical Fixation Effects In Human Cell Lines Using Raman Microspectroscopy, Aidan Meade, Colin Clarke, Florence Draux, Ganesh Sockalingum, Michel Manfait, Fiona Lyng, Hugh Byrne 2010 Technological University Dublin

Studies Of Chemical Fixation Effects In Human Cell Lines Using Raman Microspectroscopy, Aidan Meade, Colin Clarke, Florence Draux, Ganesh Sockalingum, Michel Manfait, Fiona Lyng, Hugh Byrne

Articles

The in vitro study of cellular species using Raman spectroscopy has proven a powerful non-invasive modality for the analysis of cell constituents and processes. This work uses micro-Raman spectroscopy to study the chemical fixation mechanism in three human cell lines (normal skin, normal bronchial epithelium, and lung adenocarcinoma) employing fixatives that preferentially preserve proteins (formalin), and nucleic acids (Carnoy’s fixative and methanol–acetic acid). Spectral differences between the mean live cell spectra and fixed cell spectra together with principal components analysis (PCA), and clustering techniques were used to analyse and interpret the spectral changes. The results indicate that fixation in formalin …


Investigation Of The Influence Of High Risk Human Papillomavirus On The Biochemical Composition Of Cervical Cancer Cells Using Vibrational Spectroscopy, Kamila Ostrowska, Alison Malkin, Aidan Meade, John O'Leary, Cara Martin, Cathy Spillane, Hugh Byrne, Fiona Lyng 2010 Technological University Dublin

Investigation Of The Influence Of High Risk Human Papillomavirus On The Biochemical Composition Of Cervical Cancer Cells Using Vibrational Spectroscopy, Kamila Ostrowska, Alison Malkin, Aidan Meade, John O'Leary, Cara Martin, Cathy Spillane, Hugh Byrne, Fiona Lyng

Articles

The main aetiology of cervical cancer is infection with high risk human papillomavirus (HPV). Cervical cancer is almost 100% curable if detected in the early stages. Thus, information about the presence and levels of HPV in patient samples has high clinical value. As current screening methods, such as the Pap smear test, are highly subjective and in many cases show low sensitivity and specificity, new supportive techniques are desirable to improve the quality of cervical cancer screening. In this study, vibrational spectroscopic techniques (Raman and Fourier Transform Infra Red absorption) have been applied to the investigation of four cervical cancer …


Spectroscopic And Chemometric Approaches To Radiobiological Analyses, Aidan Meade, Hugh Byrne, Fiona Lyng 2010 Technological University Dublin

Spectroscopic And Chemometric Approaches To Radiobiological Analyses, Aidan Meade, Hugh Byrne, Fiona Lyng

Articles

No abstract provided.


Functional And Pathological Analysis Of Biological Systems Using Vibrational Spectroscopy With Chemometric And Heuristic Approaches, Aidan Meade, Colin Clarke, Franck Bonnier, Kelvin W. Poon, Amaya Garcia, Peter Knief, Kamila Ostrowska, Lorenzo Salford, Haq Nawaz, Fiona Lyng, Hugh Byrne 2010 Technological University Dublin

Functional And Pathological Analysis Of Biological Systems Using Vibrational Spectroscopy With Chemometric And Heuristic Approaches, Aidan Meade, Colin Clarke, Franck Bonnier, Kelvin W. Poon, Amaya Garcia, Peter Knief, Kamila Ostrowska, Lorenzo Salford, Haq Nawaz, Fiona Lyng, Hugh Byrne

Articles

Vibrational spectroscopy (Raman and FTIR microspectroscopy) is an attractive modality for the analysis of biological samples since it provides a complete non-invasive acquisition of the biochemical fingerprint of the sample. Studies in our laboratory have applied vibrational spectroscopy to the analysis of biological function in response to external agents (chemotherapeutic drugs, ionising radiation, nanoparticles), together with studies of the pathology of tissue (skin and cervix) in health and disease. Genetic algorithms have been used to optimize spectral treatments in tandem with the analysis of the data (using generalized regression neural networks (GRNN), artificial neural networks (ANN), partial least squares modelling …


Imaging Live Cells Grown On A Three Dimensional Collagen Matrix Using Raman Microspectroscopy, Franck Bonnier, Peter Knief, Basil Lim, Aidan Meade, Jennifer Dorney, Kunal Bhattacharya, Fiona Lyng, Hugh Byrne 2010 Technological University Dublin

Imaging Live Cells Grown On A Three Dimensional Collagen Matrix Using Raman Microspectroscopy, Franck Bonnier, Peter Knief, Basil Lim, Aidan Meade, Jennifer Dorney, Kunal Bhattacharya, Fiona Lyng, Hugh Byrne

Articles

Three dimensional collagen gels have been used as matrices for the imaging of live cells by Raman spectroscopy. The study is conducted on a human lung adenocarcinoma (A549) and a spontaneously immortalized human epithelial keratinocyte (HaCaT) cell line. The lateral resolution of the system has been estimated to be


Parameters Of Reform And Unifification In Modern Japanese, R. Holzlöhner, S. M. Rochester, D. Bonaccini Calia, D. Budker, James M. Higbie, W. Hackenberg 2010 Bucknell University

Parameters Of Reform And Unifification In Modern Japanese, R. Holzlöhner, S. M. Rochester, D. Bonaccini Calia, D. Budker, James M. Higbie, W. Hackenberg

Faculty Journal Articles

No abstract provided.


Two-Way Diffusion Model For Short Exposure Holographic Grating Formation In Acrylamide-Based Photopolymer, Tzvetanka Babeva, Izabela Naydenova, Dana Mackey, Suzanne Martin, Vincent Toal 2010 Bulgarian Academy of Sciences

Two-Way Diffusion Model For Short Exposure Holographic Grating Formation In Acrylamide-Based Photopolymer, Tzvetanka Babeva, Izabela Naydenova, Dana Mackey, Suzanne Martin, Vincent Toal

Articles

A theoretical model for formation of a short exposure holographic grating is presented. The model accounts for both monomer and polymer diffusion and distinguishes between short polymer chains capable of diffusing and long polymer chains that are immobile. It is shown that the experimentally observed decrease of diffraction efficiency at higher spatial frequency can be predicted by assuming diffusion of short-chain polymers away from the bright fringes. The time evolution of the refractive index modulation after short exposure is calculated and compared with experimental results. The effects of diffusion coefficients, polymerization rates, intensity and spatial frequency of recording on the …


Study Of The Photoinduced Surface Relief Modulation In Photopolymers Caused By Illumination With A Gaussian Beam Of Light, Tzvetanka Babeva, Dana Mackey, Suzanne Martin, Izabela Naydenova, Vincent Toal 2010 Bulgarian Academy of Sciences

Study Of The Photoinduced Surface Relief Modulation In Photopolymers Caused By Illumination With A Gaussian Beam Of Light, Tzvetanka Babeva, Dana Mackey, Suzanne Martin, Izabela Naydenova, Vincent Toal

Articles

The formation of surface relief profile in photopolymerisable systems when illuminated with a focused beam of light is simulated numerically using a two-way diffusion model that takes account both for monomer and short polymer chains diffusion. The concentration and spatial distribution dynamics of monomer and short and long polymer chains are calculated. The surface profile is obtained from calculated components concentrations considering different densities of monomer and polymer. The influence of the illumination time, intensity and spot diameter on the surface profiles dynamics is discussed. A good agreement between the calculated and the experimentally measured profiles is observed thus demonstrating …


A Diffusion Model For Spatially Dependent Photopolymerization, Dana Mackey, Tzvetanka Babeva, Izabela Naydenova, VIncent Toal 2010 Technological University Dublin

A Diffusion Model For Spatially Dependent Photopolymerization, Dana Mackey, Tzvetanka Babeva, Izabela Naydenova, Vincent Toal

Articles

Photopolymers represent an attractive class of optical recording materials due to properties such as high refractive index modulation, dry film processing, long shelf life, etc. Applications include holographically based devices for optical storage disks, optical interconnections, optical memories and filters. This paper will address the dynamics of short-exposure holographic grating formation; a new mathematical model is proposed with the aim of understanding the experimental observations of low diffraction efficiency in high spatial frequency gratings.


Parton Energy Loss In Heavy-Ion Collisions Via Direct-Photon And Charged-Particle Azimuthal Correlations, B. I. Abelev, M. M. Aggarwal, Z. Ahammed, A. V. Alakhverdyants, B. D. Anderson, D. Arkhipkin, G. S. Averichev, S. Bueltmann, I. Koralt, STAR Collaboration 2010 Old Dominion University

Parton Energy Loss In Heavy-Ion Collisions Via Direct-Photon And Charged-Particle Azimuthal Correlations, B. I. Abelev, M. M. Aggarwal, Z. Ahammed, A. V. Alakhverdyants, B. D. Anderson, D. Arkhipkin, G. S. Averichev, S. Bueltmann, I. Koralt, Star Collaboration

Physics Faculty Publications

Charged-particle spectra associated with direct photon Ydir) and π0 are measured in p+p and Au+Au collisions at center-of-mass energy √sNN=200 GeV with the STAR detector at the Relativistic Heavy Ion Collider. A shower-shape analysis is used to partially discriminate between Ydirand π0. Assuming no associated charged particles in the γdir direction (near side) and small contribution from fragmentation photons (Yfrag), the associated charged-particle yields opposite to Ydir(away side) are extracted. In central Au+Au collisions, the charged-particle yields at midrapidity (|η|<1) and high transverse momentum (3 < PTassoc < 16 GeV/c) associated with γdir and π0 (|η|<0.9, 8 < PTtrig <16 GeV/c) are suppressed by a factor of 3–5 compared with p+p collisions. The observed suppression of the associated charged particles is similar for Ydir and π0 and …


Design Sensitivities Of The Superconducting Parallel-Bar Cavity, Subashini U. De Silva, Jean Delayen 2010 Old Dominion University

Design Sensitivities Of The Superconducting Parallel-Bar Cavity, Subashini U. De Silva, Jean Delayen

Physics Faculty Publications

The superconducting parallel-bar cavity has properties that makes it attractive as a deflecting or crabbing rf structure. For example it is under consideration as an rf separator for the Jefferson Lab 12 GeV upgrade and as a crabbing structure for a possible LHC luminosity upgrade. In order to maintain the purity of the deflecting mode and avoid mixing with the near accelerating mode caused by geometrical imperfection, a minimum frequency separation is needed which depends on the expected deviations from perfect symmetry. We have done an extensive analysis of the impact of several geometrical imperfections on the properties of the …


Plasma Treatment Of Bulk Niobium Surface For Superconducting Rf Cavities: Optimization Of The Experimental Conditions On Flat Samples, M. Rašković, J. Upadhyay, L. Vuskovic, S. Popovic, A.-M. Valente-Feliciano, L. Phillips 2010 Old Dominion University

Plasma Treatment Of Bulk Niobium Surface For Superconducting Rf Cavities: Optimization Of The Experimental Conditions On Flat Samples, M. Rašković, J. Upadhyay, L. Vuskovic, S. Popovic, A.-M. Valente-Feliciano, L. Phillips

Physics Faculty Publications

Accelerator performance, in particular the average accelerating field and the cavity quality factor, depends on the physical and chemical characteristics of the superconducting radio-frequency (SRF) cavity surface. Plasma based surface modification provides an excellent opportunity to eliminate nonsuperconductive pollutants in the penetration depth region and to remove the mechanically damaged surface layer, which improves the surface roughness. Here we show that the plasma treatment of bulk niobium (Nb) presents an alternative surface preparation method to the commonly used buffered chemical polishing and electropolishing methods. We have optimized the experimental conditions in the microwave glow discharge system and their influence on …


Measurement Of Single- And Double-Spin Asymmetries In Deep Inelastic Pion Electroproduction With A Longitudinally Polarized Target, H. Avakian, P. Bosted, V. D. Burkert, L. Elouadrhiri, K. P. Adhikari, M. Aghasyan, M. Amaryan, M. Anghinolfi, H. Baghdasaryan, J. Ball, G. Dodge, G. Gavalian, N. Guler, A. Klein, S E. Kuhn, D. Martinez, M. R. Niroula, F. Sabatié, H. Seraydaryan, S. Tkachenko, L. B. Weinstein 2010 Old Dominion University

Measurement Of Single- And Double-Spin Asymmetries In Deep Inelastic Pion Electroproduction With A Longitudinally Polarized Target, H. Avakian, P. Bosted, V. D. Burkert, L. Elouadrhiri, K. P. Adhikari, M. Aghasyan, M. Amaryan, M. Anghinolfi, H. Baghdasaryan, J. Ball, G. Dodge, G. Gavalian, N. Guler, A. Klein, S E. Kuhn, D. Martinez, M. R. Niroula, F. Sabatié, H. Seraydaryan, S. Tkachenko, L. B. Weinstein

Physics Faculty Publications

We report the first measurement of the transverse momentum dependence of double-spin asymmetries in semi-inclusive production of pions in deep-inelastic scattering off the longitudinally polarized proton. Data have been obtained using a polarized electron beam of 5.7 GeV with the CLAS detector at the Jefferson Lab (JLab). Modulations of single spin asymmetries over the azimuthal angle between lepton scattering and hadron production planes ϕ have been measured over a wide kinematic range in Bjorken x and virtual photon squared four-momentum Q2. A significant nonzero sin2ϕ single spin asymmetry was observed for the first time indicating strong spin-orbit correlations …


Longitudinal Scaling Property Of The Charge Balance Function In Au + Au Collisions At √Snn= 200 Gev, B. I. Abelev, M. M. Aggarwal, Z. Ahammed, A. V. Alakhverdyants, I. Alekseev, B. D. Anderson, D. Arkhipkin, G. S. Averichev, J. Balewski, L. S. Barnby, S. Bültmann, I. Koralt, D. Plyku 2010 Old Dominion University

Longitudinal Scaling Property Of The Charge Balance Function In Au + Au Collisions At √Snn= 200 Gev, B. I. Abelev, M. M. Aggarwal, Z. Ahammed, A. V. Alakhverdyants, I. Alekseev, B. D. Anderson, D. Arkhipkin, G. S. Averichev, J. Balewski, L. S. Barnby, S. Bültmann, I. Koralt, D. Plyku

Physics Faculty Publications

We present measurements of the charge balance function, from the charged particles, for diverse pseudorapidity and transverse momentum ranges in Au + Au collisions at √sNN = 200 GeV using the STAR detector at RHIC. We observe that the balance function is boost-invariant within the pseudorapidity coverage [ − 1.3 , 1.3 ] . The balance function properly scaled by the width of the observed pseudorapidity window does not depend on the position or size of the pseudorapidity window. This scaling property also holds for particles in different transverse momentum ranges. In addition, we find that the width …


Hard Photodisintegration Of A Proton Pair, I. Pomerantz, N. Bubis, K. Allada, A. Beck, B. L. Berman, W. Boeglin, A. Camsonne, M. Canan, K. Chirapatpimol, E. Cisbani 2010 Old Dominion University

Hard Photodisintegration Of A Proton Pair, I. Pomerantz, N. Bubis, K. Allada, A. Beck, B. L. Berman, W. Boeglin, A. Camsonne, M. Canan, K. Chirapatpimol, E. Cisbani

Physics Faculty Publications

We present a study of high energy photodisintegration of proton-pairs through the γ + 3He → p + p + n channel. Photon energies, , from 0.8 to 4.7 GeV were used in kinematics corresponding to a proton pair with high relative momentum and a neutron nearly at rest. The s−11 scaling of the cross section, as predicted by the constituent counting rule for two nucleon photodisintegration, was observed for the first time. The onset of the scaling is at a higher energy and the cross section is significantly lower than for deuteron (pn pair) photodisintegration. …


Investigation Of The Sources And Sinks Of Atmospheric Methane, Christopher Lee Butenhoff 2010 Portland State University

Investigation Of The Sources And Sinks Of Atmospheric Methane, Christopher Lee Butenhoff

Dissertations and Theses

The work presented here represents a number of independent studies that investigated various components of the CH4 budget, namely the sources and sinks. We used a chemical-tracer model and created unique long-term time series of atmospheric CH4, carbon monoxide (CO), molecular hydrogen (H2), and methylchloroform (CH3CCl3) measurements at marine background air to derive histories of atmospheric hydroxyl radical (OH) - the main chemical oxidant of CH4, biomass burning - an important source of CH4 in the tropics, and emissions of CH4 from rice paddies - one of …


Thin Film Group Ii-Vi Solar Cells Based On Band-Offsets, James Keith Walton 2010 Portland State University

Thin Film Group Ii-Vi Solar Cells Based On Band-Offsets, James Keith Walton

Dissertations and Theses

The amount of traditional energy sources are finite and the ecological impact of continuing to produce energy using fossil fuels will only exacerbate the carbon footprint. It is for these reasons that photovoltaic modules are becoming a larger and more necessary part the world's electricity production paradigm.

Photovoltaic (PV) semiconductor modules are grouped into three categories. 'First generation' monocrystalline and polycrystalline silicon modules that consist of p-n junctions created via the addition of impurities known as dopants. Almost 85% of solar cells produced at this time are 'first generation' and it is the high production costs of silicon PV modules …


Mesoscopic Methods In Engineering And Science, Alfons Hoekstra, Li-Shi Luo, Manfred Krafczyk 2010 Old Dominion University

Mesoscopic Methods In Engineering And Science, Alfons Hoekstra, Li-Shi Luo, Manfred Krafczyk

Mathematics & Statistics Faculty Publications

(First paragraph) Matter, conceptually classified into fluids and solids, can be completely described by the microscopic physics of its constituent atoms or molecules. However, for most engineering applications a macroscopic or continuum description has usually been sufficient, because of the large disparity between the spatial and temporal scales relevant to these applications and the scales of the underlying molecular dynamics. In this case, the microscopic physics merely determines material properties such as the viscosity of a fluid or the elastic constants of a solid. These material properties cannot be derived within the macroscopic framework, but the qualitative nature of the …


Search For The Decay Ds To Omega L Nu, Jin Zhu 2010 Wayne State University

Search For The Decay Ds To Omega L Nu, Jin Zhu

Wayne State University Dissertations

This analysis searches for a new type of rare decay of the charmed mesons,

which would provide direct evidence for the presence of Fock states inside

hadrons.

We use 586pb of e+e- collisions at the Cornell Electron Storage

Ring (CESR), at \sqrt{s}=4.17 GeV, recorded with the Cleo-c detector.

A limit on the branching ratio Ds to omega e nu is found, at 0.231\%

(95\% C.L.).


D Meson Reconstruction At Star Using The Silicon Vertex Tracker (Svt), Sarah Lapointe 2010 Wayne State University

D Meson Reconstruction At Star Using The Silicon Vertex Tracker (Svt), Sarah Lapointe

Wayne State University Dissertations

At the Relativistic Heavy Ion Collider a hot and dense matter is produced in Au+Au collisions at sqrt(sNN) = 200 GeV/c. This matter exhibits features of a new deconfined partonic matter, called the Quark-Gluon Plasma. Charm quarks are expected to be produced predominately from the initial gluon fusion in parton-parton hard scatterings. This indicates that the production of the charm occurs at the early stages of the collision. At this time the system is thought to be partonic, making the charm a powerful probe of the initial conditions. Non-photonic electron measurements in p+p, d+Au, and Au+Au provide some insight of …


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