Life Sciences Commons^™

Joint Torques And Powers Are Reduced During Ambulation For Both Limbs In Patients With Unilateral Claudication, Panagiotis Koutakis, Iraklis Pipinos, Sara A. Myers, Nikolaos Stergiou, Thomas G. Lynch, Jason Johanning

Journal Articles

Objectives: Symptomatic peripheral arterial disease (PAD) results in significant gait impairment. In an attempt to fully delineate and quantify these gait alterations, we analyzed joint kinematics, torques (rotational forces), and powers (rotational forces times angular velocity) in patients with PAD with unilateral claudication for both the affected and nonaffected legs.

Methods: Twelve patients with unilateral PAD (age, 61.69 ± 10.53 years, ankle-brachial index [ABI]: affected limb 0.59 ± 0.25; nonaffected limb 0.93 ± 0.12) and 10 healthy controls (age, 67.23 ± 12.67 years, ABI >1.0 all subjects) walked over a force platform to acquire gait kinetics, while joint kinematics were …

Micelle Enhanced Spectrofluorimetric Method For The Determination Of Ofloxacin And Lomefloxacin In Human Urine And Serum, Kuldeep Kaur, Baldev Singh, Ashok Kumar Malik

The Thai Journal of Pharmaceutical Sciences

A spectrofluorimetric method for the determination of two fluoroquinolone drugs, ofloxacin and lomefloxacin in biological fluids has been developed. The method is based on native fluorescence of these drugs in micellar medium of sodium dodecyl sulfate (S...

How Does Background And Training Affect Dance Pedagogy In Higher Education?, Meredith Erin Sims

University of Kentucky Master's Theses

Dance faculty in higher education have various backgrounds and training ranging from professional dance careers to doctoral degrees in dance. This study sought to examine the ways background and training impact faculty members‟ pedagogical approach to a dance technique class in a higher education dance department. This study examined the pedagogical knowledge, content knowledge, and pedagogical content knowledge of participants through qualitative methods including interviews, observations, and document analysis. Six major themes emerged from the data: desire to teach, teaching focus, challenging students, planning and preparation, instructional methods, and assessment strategies.

Genetic Diversity, Structure, And Recolonization Patterns Of Kentucky Black Bears, John Tyler Hast

University of Kentucky Master's Theses

After nearly a century of absence, the black bear (Ursus americanus) reappeared in Kentucky during the late 20th century and has since increased in number. Recolonization of bears in the southeastern portion of the state was thought to have been caused by emigration of bears from adjacent states into the Commonwealth, while in the south-central area, bears originated, or natural recolonization may have been supplemented by the translocation of 14 individuals into the Big South Fork National River Recreation Area. To investigate the recolonization patterns of bears in Kentucky, I used 20 microsatellite loci to determine the genetic …

Spiny Amaranth Control And Aminopyralid Persistence In Kentucky Pastures, Meghan Elizabeth Edwards

University of Kentucky Master's Theses

Spiny amaranth is a problematic weed of heavily grazed pastures in Kentucky and surrounding states. The first objective was to evaluate spiny amaranth control when herbicides are applied before and after emergence. Spiny amaranth seed collected in 2008 were seeded in rows in the fall (November) and the following spring (March) in fields located near Lexington and Princeton, KY. Treatments consisted of five application dates and five herbicides plus an untreated control arranged in a split-split plot design. The following parameters were measured: fresh weight, plant height and percent visual control. At both locations pendimethalin applied in November, March and …

Field Scale Bromide Tranport As A Function Of Precipitation Amount, Intensity And Application Time Delay, Vicente Vasquez

University of Kentucky Master's Theses

Rapid and deep transport of solutes in soils can potentially pollute groundwater resources. Field estimates of solute leaching depth based on randomized sampling provide extremely variable field average estimates that confound the treatment effects of the leaching study with the high spatial variation of soil hydraulic properties. The purpose of this study was to investigate the spatial scale of variation of solute (Bromide) leaching depth, and apply this scale of variation to study the leaching depth of Bromide as a function of a sinusoidal application of transport causing factors, i.e., rainfall amount, intensity and application time delay. Solute leaching depth …

Cover Crops And Tillage Systems For Organic Corn Production In Kentucky, Alfonso Suarez

University of Kentucky Master's Theses

Organic corn (Zea mays L.) producers generally use intensive tillage for weed control. No-till methods reduce soil erosion, conserve water, maintain soil structure and reduce CO₂ emissions. The objective of this study was test different cover crops, tillage systems, N sources and N rates for organic corn production. Two tillage systems (no-till and moldboard plow), two cover crops [hairy vetch (Vicia villosa) and rye (Secale cereale)] and two organic N sources [Louisville Green (LG) and Nature Safe (NF)] at four N rates (45, 90, 135, 180 kg N ha^-1) were evaluated …

Creating An Interactive And Dichotomous Key To The World Subfamilies Of Braconidae, Kacie Jo Johansen

University of Kentucky Master's Theses

Members of Braconidae (Hymenoptera: Ichneumonoidea) are mostly parasitoids of other holometabolous insects. It is a large family with a little over 18,000 described species and many more to be described. Subfamily classification in this group has been unstable and resolution of phylogenetic history at the subfamily level has been problematic. Since 1993, no new keys to the subfamilies of Braconidae have been produced. Many taxonomic and phylogenetic changes have taken place since then, including a recent phylogeny proposing 47 subfamilies and representing the most robust and resolved analysis to date. A pressing need for new keys to the world subfamilies …

Explorations In Homeoviscous Adaptation And Mass Spectral Analysis Of Membrane Lipids, Michael Douglas Timmons

University of Kentucky Doctoral Dissertations

The focus of this dissertation is centered on the mass spectral analysis of lipids and changes occurring in keeping with the concept of homeoviscous adaptation [1]. Homeoviscous adaptation is the process of modification of membrane lipids in response to environmental stimuli [1]. Dissertation investigations applied this concept to prokaryotic and eukaryotic organisms, and expanded the perception of environmental factors from exogenous organic solvents to intracellular environment.

The field of lipidomics deals with the analysis of phospholipid and fatty acid components of membranes the changes that occur due to environmental stimuli and their biological significance [2-6]. The high sensitivity of mass …

Improvement Of New Oil Crops For Kentucky, Watchareewan Jamboonsri

University of Kentucky Doctoral Dissertations

Three oil crops, chia (Salvia hispanica L.), flax (Linum usitatissimum L.), and castor (Ricinus communis L.), were studied because of their nutritional and industrial values. Chia and flax are rich in an ω3 fatty acid, α-linolenic acid, and castor is a very high oil producer and high in a hydroxy fatty acid. Ethyl methanesulfonate (EMS) and gamma rays were employed to mutagenize chia seeds to produce early flowering mutants. The M₁ population was grown and induced to flower by short-day photoperiods. The M₂ population was planted in the field in Lexington, KY in 2008. Early …

Studies On Formulation Development Of Apoorly Water - Soluble Drug Through Solid Dispersion Technique, Sunita Dahiya

The Thai Journal of Pharmaceutical Sciences

An attempt has been made to enhance dissolution of aceclofenac (AC) by solid dispersion technique using water soluble carriers PEG 6000 and β-cyclodextrin (β-CD). Solid dispersions of AC with PEG 6000 were prepared by melting solvent method and with β...

Use Of Charge Transfer Complexation Reaction For The Spectrophotometric Determination Of Bupropion In Pharmaceuticals And Spiked Human Urine, Kanakapura Basavaiah, Sameer A. M.

The Thai Journal of Pharmaceutical Sciences

This is the first report on use of two visible spectrophotometric methods for the determination of bupropion hydrochloride (BUPH) in pharmaceuticals and spiked human urine. Bupropion is a second-generation antidepressant agent which is also used in the m...

Improvement In The Dissolution Of Poorly Water Soluble Drug Using Media Milling Technique, Dhaval J. Patel, Jayvadan K., Vikram M. Pandya

The Thai Journal of Pharmaceutical Sciences

The rate of dissolution is rapidly becoming the leading hurdle in formulation development of poorly water-soluble drugs. In the present work, media milling was employed to formulate nanoparticles of a poorly water-soluble anti-ulcer drug, famotidine (FAM...

Author Index, Keyword Index, N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Comparing Methods Of Quantifying Tibial Acceleration Slope, Adriana Duquette, David M. Andrews

Human Kinetics Publications

Considerable variability in tibial acceleration slope (AS) values, and different interpretations of injury risk based on these values, have been reported. Acceleration slope variability may be due in part to variations in the quantification methods used. Therefore, the purpose of this study was to quantify differences in tibial AS values determined using end points at various percentage ranges between impact and peak tibial acceleration, as a function of either amplitude or time. Tibial accelerations were recorded from 20 participants (21.8 ± 2.9 years, 1.7 m ± 0.1 m, 75.1 kg ± 17.0 kg) during 24 unshod heel impacts using a …

Plasmid Injection And Application Of Electric Pulses Alter Endogenous Mrna And Protein Expression In B16.F10 Mouse Melanomas, L. C. Heller, Y. L. Cruz, B. Ferraro, H. Yang, R. Heller

Bioelectrics Publications

The application of electric pulses to tissues causes cell membrane destabilization, allowing exogenous molecules to enter the cells. This delivery technique can be used for plasmid gene therapy. Reporter gene expression after plasmid delivery with eight representative published protocols was compared in B16.F10 mouse melanoma tumors. This expression varied significantly based on the pulse parameters utilized for delivery. To observe the possible influence of plasmid injection and/or pulse application on endogenous gene expression, levels of stress-related mRNAs 4 and 24 h after delivery were determined by PCR array. Increases in mRNA levels for several inflammatory chemokines and cytokines were observed …

Electroporation-Mediated Delivery Of A Naked Dna Plasmid Expressing Vegf To The Porcine Heart Enhances Protein Expression, W. G. Marshall Jr., B. A. Boone, J. D. Burgos, S. I. Gografe, M. K. Baldwin, M. L. Danielson, M. J. Larson, D. R. Caretto, Y. Cruz, B. Ferraro, L. C. Heller, K. E. Ugen, M. J. Jaroszeski, R. Heller

Bioelectrics Publications

Gene therapy is an attractive method for the treatment of cardiovascular disease. However, using current strategies, induction of gene expression at therapeutic levels is often inefficient. In this study, we show a novel electroporation (EP) method to enhance the delivery of a plasmid expressing an angiogenic growth factor (vascular endothelial growth factor, VEGF), which is a molecule previously documented to stimulate revascularization in coronary artery disease. DNA expression plasmids were delivered in vivo to the porcine heart with or without coadministered EP to determine the potential effect of electrically mediated delivery. The results showed that plasmid delivery through EP significantly …

Increased Perfusion And Angiogenesis In A Hindlimb Ischemia Model With Plasmid Fgf-2 Delivered By Noninvasive Electroporation, B. Ferraro, Y. L. Cruz, M. Baldwin, D. Coppola, R. Heller

Bioelectrics Publications

Gene therapy approaches delivering fibroblast growth factor-2 (FGF-2) have shown promise as a potential treatment for increasing blood flow to ischemic limbs. Currently, effective noninvasive techniques to deliver plasmids encoding genes of therapeutic interest, such as FGF-2, are limited. We sought to determine if intradermal injection of plasmid DNA encoding FGF-2 (pFGF) followed by noninvasive cutaneous electroporation (pFGFE+) could increase blood flow and angiogenesis in a rat model of hindlimb ischemia. pFGFE+ or control treatments were administered on postoperative day 0. Compared to injection of pFGF alone (pFGFE-), delivery of pFGFE+ significantly increased FGF-2 expression for 10 days. Further, the …

Frustrated Drift Of An Anchored Scroll-Wave Filament And The Geodesic Principle, Marcel Wellner, Christian W. Zemlin, Arkady M. Pertsov

Bioelectrics Publications

We investigate anchored scroll-wave filaments in an excitable medium whose diffusivity matrix, including its determinant, is spatially nonuniform. The study is motivated by cardiological applications where scroll-wave behavior in the presence of diffusivity gradients is believed to play an important role in the development of severe arrhythmias. A diffusivity gradient is expected to make the filament drift, unless drift is prevented ("frustrated") by anchoring to localized defects in the propagation medium. The resulting stationary filament is a geodesic curve, as demonstrated here in the case of a nonzero but constant gradient. That is, the diffusivity matrix has a determinant that …

Brown Alga Sargassum Muticum Inhibits Proinflammatory Cytokines, Inos, And Cox-2 Expression In Macrophage Raw 264.7 Cells, Weon-Jong Yoon, Wook Jae Lee, Nam Ho Lee, Chang-Gu Hyun

Turkish Journal of Biology

The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. The extract was then successively partitioned with n-hexane, CH_2Cl_2, EtOAc, BuOH, and water. The results indicate that the CH_2Cl_2 fraction of S. muticum extract was an effective inhibitor of LPS-induced NO and PGE_2 production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of S. muticum included dose-dependent decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA. To test the inhibitory effects …