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Electric Pulses To Prepare Feeder Cells For Sustaining And Culturing Of Undifferentiated Embryonic Stem Cells, Lauren M. Browning, Tao Huang, Xiao-Hong Nancy Xu 2010 Old Dominion University

Electric Pulses To Prepare Feeder Cells For Sustaining And Culturing Of Undifferentiated Embryonic Stem Cells, Lauren M. Browning, Tao Huang, Xiao-Hong Nancy Xu

Chemistry & Biochemistry Faculty Publications

Current challenges in embryonic-stem-cell (ESC) research include inability of sustaining and culturing of undifferentiated ESCs over time. Growth-arrested feeder cells are essential to the culture and sustaining of undifferentiated ESCs, and they are currently prepared using gammaradiation and chemical inactivation. Both techniques have severe limitations. In this study, we developed a new, simple and effective technique (pulsed-electric-fields, PEFs) to produce viable growth-arrested cells (RTS34st) and used them as high-quality feeder cells to culture and sustain undifferentiated zebrafish ESCs over time. The cells were exposed to 25 sequential 10- nanosecond-electric-pulses (10nsEPs) of 25, 40 and 150 kV/cm with 1s pulse interval, …


Forage News [2010-01], Department of Plant and Soil Sciences, University of Kentucky 2010 University of Kentucky

Forage News [2010-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

  • Forages at KCA
  • Heart of America Grazing Conference
  • 30th Anniversary Alfalfa Conference
  • Tall Fescue for the Twenty-First Century
  • Grazing Conference Photos
  • Hay Testing
  • Growing Alfalfa in the South
  • New Forage Variety Test Reports
  • American Grassfed Association Coming Back to Lexington
  • Consider Joining KFGC in 2010
  • Comment Period on RR Alfalfa - December 18, 2009 - February 16, 2010


Determination Of Optimal Dose Of Egf For Bovine Oocyte Maturation And Subsequent In Vitro Fertilization And Culture In Two Media, AYHAN BAŞTAN, BÜLENT POLAT, DUYGU BAKİ ACAR, ÖMER KORKMAZ, ARMAĞAN ÇOLAK 2010 TÜBİTAK

Determination Of Optimal Dose Of Egf For Bovine Oocyte Maturation And Subsequent In Vitro Fertilization And Culture In Two Media, Ayhan Baştan, Bülent Polat, Duygu Baki̇ Acar, Ömer Korkmaz, Armağan Çolak

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the optimal dose of EGF on in vitro maturation of bovine oocytes and to investigate the effect of different IVF and IVC media on development of bovine embryos matured with EGF. Oocytes collected from a slaughterhouse were randomly divided into 4 groups. Each group of oocytes was incubated with different doses of EGF (Control: 0 ng/mL, Group 1: 1 ng/mL, Group 2: 10 ng/mL, and Group 3: 100 ng/mL) for 24 h to determine the optimal dose. Matured oocytes were fertilized with frozen-thawed bull sperm by using the swim-up method. The oocytes …


Effects Of Supplemental Fructo-Oligosaccharide And Mannan-Oligosaccharide On Nutrient Digestibilities, Volatile Fatty Acid Concentrations, And Immune Function In Horses, EMEL GÜRBÜZ, FATMA İNAL, SAKİNE ÜLKÜM ATA, ÖZCAN BARIŞ ÇİTİL, KÜRŞAT KAV, FATİH KÜÇÜKKAYA 2010 TÜBİTAK

Effects Of Supplemental Fructo-Oligosaccharide And Mannan-Oligosaccharide On Nutrient Digestibilities, Volatile Fatty Acid Concentrations, And Immune Function In Horses, Emel Gürbüz, Fatma İnal, Saki̇ne Ülküm Ata, Özcan Bariş Çi̇ti̇l, Kürşat Kav, Fati̇h Küçükkaya

Turkish Journal of Veterinary & Animal Sciences

This research was performed to study the effects of fructo-oligosaccharide (FOS) and mannan-oligosaccharide (MOS) on nutrient digestibilities, fecal pH and volatile fatty acids compositions, and immune function in adult horses. Four adult Thoroughbred horses were used in a 4×4 Latin Square design with 20-day treatment periods. A 12-day adaptation phase was followed by 6-day collection of feces and 2-day blood sampling phases. Four different treatments were administered: 1) Control (no supplemental FOS or MOS; 2) 30 g of FOS/d; 3) 30 g of MOS/d; 4) 15 g of FOS+15 g of MOS/d. There were no differences among treatments in the …


Determination Of Microbial Contamination Sources At A Frankfurter Sausage Processing Line, EBRU GÜNGÖR, NALAN GÖKOĞLU 2010 TÜBİTAK

Determination Of Microbial Contamination Sources At A Frankfurter Sausage Processing Line, Ebru Güngör, Nalan Gökoğlu

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine microbial contamination sources during sausage processing. The samples were taken at 6 different stages of the sausage processing line in a local plant. Minced meat, sausage batter, stuffed sausage, cooked sausage, peeled sausage, and pasteurized sausage samples were examined microbiologically. Moreover, spices and ice water used in production, personnel hands, and equipment were examined. Counts of total aerobic mesophilic bacteria, Staphylococcus aureus, Escherichia coli, yeasts, and molds in minced meat were found to be 7.02, 3.83, 4.42, and 1.62 log cfu/g, respectively. E. coli and yeast-mold counts in sausage batter reached 3.99 and 1.72 …


Investigation Of Antimicrobial Susceptibility For Enterococci Isolated From Cats And Dogs And The Determination Of Resistance Genes By Polymerase Chain Reaction, SÜHEYLA TÜRKYILMAZ, VASFİYE ERDEM, BÜLENT BOZDOĞAN 2010 TÜBİTAK

Investigation Of Antimicrobial Susceptibility For Enterococci Isolated From Cats And Dogs And The Determination Of Resistance Genes By Polymerase Chain Reaction, Süheyla Türkyilmaz, Vasfi̇ye Erdem, Bülent Bozdoğan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the antimicrobial susceptibility and to determinate the resistance genes by Polymerase Chain Reaction (PCR) among enterococci isolated from healthy cats and dogs in İzmir, Turkey. From a total of 114 rectal swabs, 91 (31 cats and 60 dogs) stains were isolated as enterococci. Twenty eight isolates were identified as Enterococcus faecalis and 63 as Enterococcus faecium with the API 20 IDStrep system. Antimicrobial susceptibilities were determined by disc diffusion. Susceptibility rates of all enterococci strains were found as 100.0%, 95.6%, 94.5%, 87.9%, 84.6%, 30.8%, 29.7%, and 0.0% for teicoplanin, penicillin, vancomycine, chloramphenicol, …


The Effects Of Dietary Ascorbic Acid Supplementation On Collagen And Amino Acid Concentrations In Japanese Quails Exposed To Heat Stress, KAMİL SEYREK, HAKAN ERBAŞ, MUKADDER SERTER, ÇİĞDEM YENİSEY, FUNDA KIRAL, HÜSNÜ ERBAY BARDAKÇIOĞLU 2010 TÜBİTAK

The Effects Of Dietary Ascorbic Acid Supplementation On Collagen And Amino Acid Concentrations In Japanese Quails Exposed To Heat Stress, Kami̇l Seyrek, Hakan Erbaş, Mukadder Serter, Çi̇ğdem Yeni̇sey, Funda Kiral, Hüsnü Erbay Bardakçioğlu

Turkish Journal of Veterinary & Animal Sciences

A considerable body of data exists regarding the role of vitamin C in mammalian physiology; however, there are no data about the effects of dietary ascorbic acid supplementation on collagen concentrations and amino acid levels in animals exposed to heat stress. The present study investigated the effects of supplementary ascorbic acid intake on collagen concentrations in the brain and heart tissue in Japanese quails. In addition, glycine, glutamine, histidine, asparagine, and serine contents in the livers of the same animals were measured. Japanese quails were allocated into 4 groups, each of which was exposed to heat stress (34.8 ± 1.25 …


Interaction Between Particle Sizes Of Alfalfa Hay And Corn Grain On Milk Yield, Milk Composition, Chewing Activity, And Ruminal Ph Of Dairy Cows, MEHMET ALİ BAL, E. BANU BÜYÜKÜNAL BAL 2010 TÜBİTAK

Interaction Between Particle Sizes Of Alfalfa Hay And Corn Grain On Milk Yield, Milk Composition, Chewing Activity, And Ruminal Ph Of Dairy Cows, Mehmet Ali̇ Bal, E. Banu Büyükünal Bal

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to determine the interactions between alfalfa hay and corn grain particle sizes on nutrient intake, milk yield, milk composition, chewing activity, and ruminal pH of dairy cows. Alfalfa hay was chopped either at 4 cm (long; L) or 1 cm (short; S) theoretical length of cut. Particle size of corn grain was obtained by grinding either at 1 mm (coarse; C) or less than 1 mm (fine; F) mean particle size. Four lactating multiparous Holstein cows were assigned in a 4×4 Latin Square design in a 2×2 factorial arrangement of treatments with 21-day periods. …


A Case Study Of The Clinical Form Of Johne’S Disease In A Heifer, AGNIESZKA WISZNIEWSK LASZCZYCH, JOANNA SZTEYN, ALEKSANDRA RUSZCZYNSKA 2010 TÜBİTAK

A Case Study Of The Clinical Form Of Johne’S Disease In A Heifer, Agnieszka Wiszniewsk Laszczych, Joanna Szteyn, Aleksandra Ruszczynska

Turkish Journal of Veterinary & Animal Sciences

Johne's disease-paratuberculosis-affects domesticated and wild ruminants throughout the world. The first symptoms of the disease appear 6 months to 15 years after infection. The clinical stage of paratuberculosis usually occurs in older animals and is rare in those under 2 years of age. While performing analyses on a farm in the northeastern region of Poland a 2-year-old heifer that manifested symptoms of dyspepsia, proceeding with watery diarrhea, was observed. ELISA test (IDEXX) results demonstrated a positive serological reaction for paratuberculosis. Clinical examination, microbiological analysis of feces, autopsy, and histopathological examination confirmed the incidence of Johne's disease in the heifer.


Responses To Abrupt Changes In Feeding And Illumination In Laying Hens, ASHRAF M. KHALIL, KANJI MATSUI, KEN-ICHI TAKEDA 2010 TÜBİTAK

Responses To Abrupt Changes In Feeding And Illumination In Laying Hens, Ashraf M. Khalil, Kanji Matsui, Ken-Ichi Takeda

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate the responses to abrupt changes in feeding and illumination during the egg-laying period. Six hens were housed individually in cages under constant environmental conditions, with a photoperiod of 15 h (0400-1900) and ad libitum access to food for 10 days. Then the same hens were subjected to a feed withdrawal trial (between 1200 and 0830), followed by a 5-h reduction in the photoperiod (0400-1400). The heart rate (HR), body temperature (BT), and locomotor activity (LA) of the laying hens were measured continuously using radiotelemetry, with simultaneous recording of the time of oviposition. Behavioral responses to …


Prevalence And Antimicrobial Resistance Of Salmonella Serovars Isolated From Poultry Meat In Hyderabad, Pakistan, AIJAZ HUSSAIN SOOMRO, MUHAMMAD KHASKHELI, MUHAMMAD BACHAL BHUTTO, GHIASUDDIN SHAH, AZIZULLAH MEMON, PARKASH DEWANI 2010 TÜBİTAK

Prevalence And Antimicrobial Resistance Of Salmonella Serovars Isolated From Poultry Meat In Hyderabad, Pakistan, Aijaz Hussain Soomro, Muhammad Khaskheli, Muhammad Bachal Bhutto, Ghiasuddin Shah, Azizullah Memon, Parkash Dewani

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the prevalence of Salmonella in broiler chicken meat sold in Hyderabad, Pakistan. A total of 100 samples were randomly collected from poultry meat retail markets and examined for the presence of Salmonella. The prevalence rate recorded was 38%. The most prevalent serogroups were S. enteritidis, S. typhi, S. pullorum, and S. typhimurium. All the Salmonella isolates showed resistance to ampicillin, and sensitivity to tetracycline, streptomycin, cefotaxime, ceftazidime, gentamicin, tobramycin, ciprofloxacin, ofloxacin, and chloramphenicol. The findings highlighted the magnitude of Salmonella contamination in chicken meat sold in the city, and the antibiotic resistance of Salmonella …


Bilateral Abscesses Of The Maxillary Carnassial Teeth In A Female Pekinese, ABUTORAB TABATABAEI NAEINI, ALIREZA RAAYAT JAHROMI, SAMIRA MEHRSHAD 2010 TÜBİTAK

Bilateral Abscesses Of The Maxillary Carnassial Teeth In A Female Pekinese, Abutorab Tabatabaei Naeini, Alireza Raayat Jahromi, Samira Mehrshad

Turkish Journal of Veterinary & Animal Sciences

Tooth infections are typically seen in older dogs as a result of poor oral hygiene (periodontal disease) or traumatic tooth fractures (endodontic and periapical disease). An 8-year-old female Pekinese was presented due to bilateral draining tracts located just under its eyes, and a history of healing and recurrence of the lesions after about 3 weeks of systemic antibiotic therapy. Dental radiography revealed bilateral carnassial tooth root abscesses. Both teeth were extracted under general anesthesia. With proper postoperative care the abscesses healed with no further complications.


First Report Of Canine Visceral Leishmaniasis In Bulgaria, ILIA TSACHEV, IOANNIS D. KYRIAZIS, SOFIA BOUTSINI, EVDOKIA KARAGOUNI, ELENI DOTSIKA 2010 TÜBİTAK

First Report Of Canine Visceral Leishmaniasis In Bulgaria, Ilia Tsachev, Ioannis D. Kyriazis, Sofia Boutsini, Evdokia Karagouni, Eleni Dotsika

Turkish Journal of Veterinary & Animal Sciences

Seroepidemiological studies on canine leishmaniasis in Bulgaria, even in areas where human leishmaniasis has been detected, are controversial. This report describes 2 clinical cases of canine visceral leishmaniasis. The disease was confirmed by microscopical, serological, and molecular methods. It is, therefore, suggested that infected dogs could constitute the reservoir for human leishmaniasis in the country.


"A Redescription Of Potamonautes Loveni, A Medically Important Feshwater Crab From Western Kenya And Eastern Uganda, East Africa" , Neil Cumberlidge, Paul F. Clark 2010 Northern Michigan University

"A Redescription Of Potamonautes Loveni, A Medically Important Feshwater Crab From Western Kenya And Eastern Uganda, East Africa" , Neil Cumberlidge, Paul F. Clark

Journal Articles

No abstract provided.


Nominal Schemas For Integrating Rules And Ontologies, Frederick Maier, Adila A. Krisnadhi, Pascal Hitzler 2010 Wright State University - Main Campus

Nominal Schemas For Integrating Rules And Ontologies, Frederick Maier, Adila A. Krisnadhi, Pascal Hitzler

Computer Science and Engineering Faculty Publications

We propose a description-logic style extension of OWL DL, which includes DL-safe variable SWRL and seamlessly integrates datalog rules. Our language also sports a tractable fragment, which we call ELP 2, covering OWL EL, OWL RL, most of OWL QL, and variable restricted datalog.


Validation Of Live Animal Ultrasonic Measurements Of Body Composition In Market Lambs, J. C. Emenheiser, S. P. Greiner, Ronald M. Lewis, D. R. Notter 2010 Virginia Polytechnic Institute and State University

Validation Of Live Animal Ultrasonic Measurements Of Body Composition In Market Lambs, J. C. Emenheiser, S. P. Greiner, Ronald M. Lewis, D. R. Notter

Faculty Papers and Publications in Animal Science

Market lambs from the state fair of Virginia (n = 172) were ultrasonically evaluated by 4 scan technicians and 3 image interpreters to determine accuracy of ultrasonic estimates of loin muscle area (ULMA), backfat thickness (UBF), and body wall thickness (UBW). Lambs were initially scanned at the preferred magnification setting of each technician; 2 chose 1.5× and 2 chose 2.0×. Lambs were then scanned a second time for ULMA and UBF with machine magnification settings changed from 1.5 to 2.0×, or vice versa, midway through the second scan. Lambs were then slaughtered, and analogous measurements [carcass loin muscle area, …


Feed Intake Of Sheep As Affected By Body Weight, Breed, Sex, And Feed Composition, R. M. Lewis, G. C. Emmans 2010 Scottish Agricultural College

Feed Intake Of Sheep As Affected By Body Weight, Breed, Sex, And Feed Composition, R. M. Lewis, G. C. Emmans

Faculty Papers and Publications in Animal Science

The hypotheses tested were that genetic size-scaling for mature BW (A, kg) would reduce variation in intake between kinds of sheep and that quadratic polynomials on u = BW/A with zero intercept would provide good descriptions of the relationship between scaled intake (SI, g/A0.73 d) and degree of maturity in BW (u) across feeds of differing quality. Both sexes of Suffolk sheep from 2 experimental lines (n = 225) and from 3 breed types (Suffolk, Scottish Blackface, and their cross; n = 149) were recorded weekly for ad libitum feed intake and BW; recording of intake was from weaning through, …


Detection, Validation, And Downstream Analysis Of Allelic Variation In Gene Expression, Daniel C. Ciobanu, Lu Engle, Khyobeni Mozhui, Xusheng Wang, Manjunatha Jagalur, John A. Morris, William L. Taylor, Klaus Dietz, Perikles Simon, Robert W. Williams 2010 University of Nebraska-Lincoln

Detection, Validation, And Downstream Analysis Of Allelic Variation In Gene Expression, Daniel C. Ciobanu, Lu Engle, Khyobeni Mozhui, Xusheng Wang, Manjunatha Jagalur, John A. Morris, William L. Taylor, Klaus Dietz, Perikles Simon, Robert W. Williams

Faculty Papers and Publications in Animal Science

Common sequence variants within a gene often generate important differences in expression of corresponding mRNAs. This high level of local (allelic) control—or cis modulation—rivals that produced by gene targeting, but expression is titrated finely over a range of levels. We are interested in exploiting this allelic variation to study gene function and downstream consequences of differences in expression dosage. We have used several bioinformatics and molecular approaches to estimate error rates in the discovery of cis modulation and to analyze some of the biological and technical confounds that contribute to the variation in gene expression profiling. Our analysis of SNPs …


Tympanic Temperature In Confined Beef Cattle Exposed To Excessive Heat Load, Terry L. Mader, John B. Gaughan, Leslie J. Johnson, G. Leroy Hahn 2010 University of Nebraska - Lincoln

Tympanic Temperature In Confined Beef Cattle Exposed To Excessive Heat Load, Terry L. Mader, John B. Gaughan, Leslie J. Johnson, G. Leroy Hahn

Faculty Papers and Publications in Animal Science

Angus crossbred yearling steers (n=168) were used to evaluate effects on performance and tympanic temperature (TT) of feeding additional potassium and sodium to steers exposed to excessive heat load (maximum daily ambient temperature exceeded 32°C for three consecutive days) during seasonal summer conditions. Steers were assigned one of four treatments: (1) control; (2) potassium supplemented (diet containing 2.10% KHCO3); (3) sodium supplemented (diet containing 1.10% NaCl); or (4) potassium and sodium supplemented (diet containing 2.10% KHCO3 and 1.10% NaCl). Overall, additional KHCO3 at the 2% level or NaCl at the 1% level did not improve performance or heat stress tolerance …


Development And Usage Of Extension’S Horsequest: An Online Resource, E. A. Greene, A. S. Griffin, J. Whittle, C. A. Williams, A. B. Howard, Kathleen P. Anderson 2010 University of Vermont

Development And Usage Of Extension’S Horsequest: An Online Resource, E. A. Greene, A. S. Griffin, J. Whittle, C. A. Williams, A. B. Howard, Kathleen P. Anderson

Faculty Papers and Publications in Animal Science

eXtension (pronounced e-extension) is an online resource transforming how faculty can collaborate and deliver equine education. As the first Community of Practice launched from eXtension, HorseQuest (HQ) offers free, interactive, peer-reviewed, online resources on a variety of equine-related topics at http://www.extension.org. This group has adapted traditional educational content to the online environment to maximize search engine optimization, to be more discoverable and relevant in the online world. This means that HQ resources are consistently being found on the first page of search results. Also, by researching key words searched by Internet users, HQ has guided new content direction and …


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