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Survey Of The Bolctes From Fish River Nature Preserve, Noah T. Nelson 2024 University of South Alabama

Survey Of The Bolctes From Fish River Nature Preserve, Noah T. Nelson

Honors Theses

Boletes are a special kind of mushroom inside of the Kingdom Fungi that are distinguished from other mushrooms by their uniquely pored hymenium, as opposed to gills. Many boletes are ectomycorrhizal, meaning they are mutualists with vascular plants, and thus beneficial to the overall health of the terrestrial ecosystem. Some are well-known for their edibility and are of economic importance. In the United States 59 genus-level clades comprising 290 operational taxonomic units have been reported. The southeastern US and Gulf Coast regions however are less researched and recorded for their mushroom ecology. In Alabama, only 56 bolete species have been …


Analysis Of Water Pollutants Across Greater Omaha, Desmond Marshall 2024 University of Nebraska at Omaha

Analysis Of Water Pollutants Across Greater Omaha, Desmond Marshall

Theses/Capstones/Creative Projects

Water is a finite resource, and most water has the potential to become contaminated in sitting bodies. Health complications have an increased chance of occurring when these water sources have pollutants. Preventative and treatment methods are components of the legislation put in place to prevent concentrations from reaching levels capable of causing these ailments. However, sites sometimes fail to keep them under tolerated levels. This study is intended to examine if several locations across Omaha are keeping pollutants under legal concentrations and analyze any trends in the data. Locations (n=10) are sampled twice, with exactly two-weeks between collections. The water …


Bcl2 Mediated Targeted Drug Delivery For The Treatment Of Kidney Fibrosis And Stomach Cancer, Humayra Afrin 2024 University of Texas at El Paso

Bcl2 Mediated Targeted Drug Delivery For The Treatment Of Kidney Fibrosis And Stomach Cancer, Humayra Afrin

Open Access Theses & Dissertations

Apoptosis, the programmed death of cells, is primarily regulated by a delicate balance between pro-apoptotic and anti-apoptotic signals. The Bcl-2 (B-cell lymphoma 2) family of proteins acts as anti-apoptotic agents, promoting cell survival. Dysregulation of these proteins is a common occurrence in conditions such as cancer and fibrosis, where overexpression of anti-apoptotic members can foster tumor cell survival and fibroblast activation. In this study, our aim was to explore the therapeutic potential of Bcl-2 inhibitors, both as a small molecule (specifically Navitoclax (Navi)), inhibitor and as Bcl-2 siRNA, for targeted treatment. Intravenous administration of Navi often leads to thrombocytopenia, necessitating …


Conserving One Of Hawai'i'S Last Endemic Ducks: Genetics And Habitat Associations Of Koloa, Feral Mallards, And Their Hybrids, Kristi Fukunaga 2024 University of Texas at El Paso

Conserving One Of Hawai'i'S Last Endemic Ducks: Genetics And Habitat Associations Of Koloa, Feral Mallards, And Their Hybrids, Kristi Fukunaga

Open Access Theses & Dissertations

Increases in anthropogenic hybridization through introduced species have accelerated the loss of genetic diversity and reductions in population size of native species that are already threatened by population and genetic diversity decline. Fertile hybrids that are common among waterfowl (order Anseriformes) are especially worrisome. A prime example is the endangered Hawaiian duck (Anas wyvilliana; “koloa maoli”), which is the remaining endemic duck species on the main Hawaiian Islands and is threatened by genetic extinction through ongoing hybridization with feral mallards (Anas platyrhynchos). Of note, koloa populations are known to be strongly male-biased (3:1), and this sex bias is known to …


Prelimbic Cortex Neuronal Assemblies In Behaviors Predictive Of Drug-Seeking Before And After Cocaine Self-Administration, Karla J. Galvan 2024 University of Texas at El Paso

Prelimbic Cortex Neuronal Assemblies In Behaviors Predictive Of Drug-Seeking Before And After Cocaine Self-Administration, Karla J. Galvan

Open Access Theses & Dissertations

Literature has established that there are certain behaviors that are predictive of drug seeking and relapse such as impulsivity, distress tolerance (DT), Pavlovian conditioned approach (PCA), anxiety, and sensation-seeking. However, few studies have examined the behavioral interactions among these tasks and drug-seeking, and none have examined the neural interactions in the prelimbic cortex, which has an essential role in drug-seeking and is implicated in each of the aforementioned behaviors of interest.We therefore hypothesized that certain behaviors including high impulsivity, low distress tolerance, high anxiety, and high locomotor activity would predict high cocaine-seeking. We further hypothesized that the neuronal activity in …


Synthesis Of Neoglycoproteins As Potential Biomarkers For Chagas Disease And Cutaneous Leishmaniasis Caused By Leishmania (Viannia) Braziliensis, Eileni Rodriguez Gil 2024 University of Texas at El Paso

Synthesis Of Neoglycoproteins As Potential Biomarkers For Chagas Disease And Cutaneous Leishmaniasis Caused By Leishmania (Viannia) Braziliensis, Eileni Rodriguez Gil

Open Access Theses & Dissertations

Protozoan parasites are responsible for causing numerous diseases in humans and mammals worldwide. Among them are Chagas disease (CD), caused by the parasite Trypanosoma cruzi (T. cruzi), and tegumentary leishmaniasis (TL), caused by the parasite Leishmania (Viannia). Both diseases coexist in many regions of Latin America, and current hurdles to differentially diagnose them and determine chemotherapy success need to be overcome. CD can be diagnosed by traditional serological tests and polymerase chain reaction (PCR), but disadvantages of these methods are that they may produce false negatives because the antigens used in commercial CD kits are not conserved across all T.cruzi …


Atlas-Based Analysis Of The Neural Projections From The Lateral Hypothalamic Area To The Lower Brainstem In The Adult Male Rat, Jessica Valeria Salcido Padilla 2024 University of Texas at El Paso

Atlas-Based Analysis Of The Neural Projections From The Lateral Hypothalamic Area To The Lower Brainstem In The Adult Male Rat, Jessica Valeria Salcido Padilla

Open Access Theses & Dissertations

Within the last decade, the number of diseases associated with dysregulation of the neural circuits responsible for energy balance, glucosensing, and feeding control has increased. One brain region containing these circuits is the lateral hypothalamic area (LHA), which plays an important role in the sleep/wake cycle, energy balance, and motivated behavior. Although the LHA has been termed a â??feeding centerâ??, other regions within the hindbrain are known for the regulation of similar functions including energy balance, glucosensing, and feeding control. While many studies have explored and reported projections from the LHA to the hindbrain, few have further investigated the interconnections …


Stopping The Bleed At Uri, Emily Lefebre 2024 University of Rhode Island

Stopping The Bleed At Uri, Emily Lefebre

Senior Honors Projects

The number one cause of preventable death is uncontrolled bleeding from traumatic injury. From the unlikely scenario of a school shooting, to a campus car accident or an injury during a sports or club event, possessing skills to effectively stop a life-threatening bleed becomes a matter of life or death. The American College of Surgeons Committee on Trauma (ACS COT) STOP THE BLEED® program is a campaign to teach communities how to respond effectively and efficiently to life-threatening bleeding in emergencies. STOP THE BLEED® aims to empower individuals with the knowledge and skills to stop severe bleeding by tourniquet application, …


Testing Functional Homology Of The Lga2 And Rga2 Genes Of Sporisorium Reilianum In Ustilago Maydis, Joshua Thomas 2024 University of Louisville

Testing Functional Homology Of The Lga2 And Rga2 Genes Of Sporisorium Reilianum In Ustilago Maydis, Joshua Thomas

College of Arts & Sciences Senior Honors Theses

Ustilago maydis, a basidiomycete fungus, infects corn and displays dimorphism, transitioning between nonpathogenic and pathogenic stages. Its life cycle involves mating of haploids with different alleles, resulting in the formation of galls/tumors filled with teliospores. The a1 and a2 alleles regulate pheromone recognition and mating. The lga2 and rga2 genes in the a2 allele are crucial for uniparental mitochondrial inheritance. Sporisorium reilianum, a related fungus, has homologs of lga2 and rga2 with U. maydis. To test the functional homology of the S. reilianum genes, protoplasts of U. maydis strains deleted for lga2 or lga2 and rga2 were transformed …


The Anti-Diabetic Drug Metformin Disrupts Feeding And Sleeping Behaviors In Drosophila Melanogaster., Lucas Fitzgerald 2024 University of Louisville

The Anti-Diabetic Drug Metformin Disrupts Feeding And Sleeping Behaviors In Drosophila Melanogaster., Lucas Fitzgerald

College of Arts & Sciences Senior Honors Theses

Dimethylbiguanide, also known as metformin, is the single most prescribed oral treatment for non-insulin dependent diabetes mellitus, or type 2 diabetes, in Western countries. The primary mechanism of action that metformin acts through is the activation of AMP kinase, an important regulator of energy homeostasis. While the anti-diabetic effects of metformin are well documented, its effects on feeding and sleeping behaviors are not well characterized. Using the model organism Drosophila melanogaster, the mean daily quantity of food consumed was measured and compared between groups treated with several dosages of metformin. Feeding interactions such as meal frequency and length were …


Gopher Tortoises In Protected Natural Areas: Effects Of Natural Area Characteristics On Populations, Taryn Lagor 2024 Lynn University

Gopher Tortoises In Protected Natural Areas: Effects Of Natural Area Characteristics On Populations, Taryn Lagor

Graduate Student Dissertations, Theses, Capstones, and Portfolios

The gopher tortoise (Gopherus polyphemus) is native to the Southeast region of the United States. The burrows it digs supports the survival and wellbeing of over 350 other species, with over 60 of those being other vertebrates, making it a keystone species. It is ranked threated to rare by differing organizations with the main threat throughout its entire natural range being habitat loss. The Florida Scrub is the natural habitat of Florida gopher tortoise populations and is one of the fastest declining ecosystems in Florida, particularly in South Florida, due to rapid urban development. Many of the remaining …


The Optimal Timing Of Abscisic Acid Treatment In Arabidopsis Under Drought And Salt Stress, Khelia Gihozo 2024 Lynn University

The Optimal Timing Of Abscisic Acid Treatment In Arabidopsis Under Drought And Salt Stress, Khelia Gihozo

Graduate Student Dissertations, Theses, Capstones, and Portfolios

As climate change intensifies, harsh environmental conditions, such as increased drought and salt levels, will pose significant challenges to agricultural productivity globally. It is important to develop strategies for plants to tolerate these adverse conditions. This study aims to investigate how well the timing of abscisic acid (ABA) treatment can promote resistance to salt and drought in Arabidopsis thaliana grown on Murashige and Skoog agar medium. The study evaluates both wild-type and transgenic lines overexpressing the DREB2A gene, a transcriptional activator involved in the stress response of plants. Plants were exposed to drought and salt stress as they matured. Followed …


Reactive Oxygen Species Production In Head Regeneration Of Planaria From Cadmium Exposure, Natalie Gonzalez 2024 Lynn University

Reactive Oxygen Species Production In Head Regeneration Of Planaria From Cadmium Exposure, Natalie Gonzalez

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Exposure to cadmium, a pervasive neurotoxic chemical found in our environment, poses significant health risks, disrupting various physiological systems and fostering carcinogenesis. Commonly found in everyday items like cigarettes, batteries, and plastics, cadmium disrupts the equilibrium between reactive oxygen species (ROS) production and cellular repair mechanisms, culminating in oxidative stress and potential cell damage. In toxicological and pharmaceutical research, planarians offer valuable insight for understanding how exposure to chemicals akin to human scenarios can alter biological systems. This experiment addresses the effect cadmium chloride (CdCl2) has on planarian regeneration and whether antioxidants can mitigate those adverse outcomes. Our …


Survey Of The Boletes From Fish River Nature Preserve, Noah T. Nelson 2024 University of South Alabama

Survey Of The Boletes From Fish River Nature Preserve, Noah T. Nelson

Poster Presentations

Boletes are a special kind of mushroom inside of the Kingdom Fungi that are distinguished from other mushrooms by their uniquely pored hymenium, as opposed to gills. Many boletes are ectomycorrhizal, meaning they are mutualists with vascular plants, and thus beneficial to the overall health of the terrestrial ecosystem. Some are well-known for their edibility and are of economic importance. In the United States 59 genus-level clades comprising 290 operational taxonomic units have been reported. The southeastern US and Gulf Coast regions however are less researched and recorded for their mushroom ecology. In Alabama, only 56 bolete species have been …


Spatiotemporal Gait Parameter Consistency Across Two Days Of Treadmill Walking In Stroke Survivors, Alejandro Aguirre Ramirez, Samantha N. Jeffcoat, Natalia Sanchez, Andrian Kuch 2024 Chapman University

Spatiotemporal Gait Parameter Consistency Across Two Days Of Treadmill Walking In Stroke Survivors, Alejandro Aguirre Ramirez, Samantha N. Jeffcoat, Natalia Sanchez, Andrian Kuch

Student Scholar Symposium Abstracts and Posters

Retraining impaired gait is essential in post-stroke recovery. This project aims to determine between day variability of spatiotemporal gait measures in treadmill walking post-stroke to differentiate between changes due to the intervention and measurement errors due to between day test-retest variability. Six individuals post-stroke performed a two-minute walk test at a self-selected speed (SSS) for two consecutive days. SSS was assessed through the six-minute walk test. Reflective markers were placed on anatomical landmarks (van den Bogert et al. 2013). The heel markers were used for step detection. We calculated spatiotemporal parameters: stride length, stride time, step length, stance time, and …


Season Of Grazing Interacts With Soil Texture, Selecting For Associations Of Biocrust Morphogroups, Lea A. Condon, Roger Rosentreter, Kari E. Veblen, Peter S. Coates 2024 U.S. Geological Survey

Season Of Grazing Interacts With Soil Texture, Selecting For Associations Of Biocrust Morphogroups, Lea A. Condon, Roger Rosentreter, Kari E. Veblen, Peter S. Coates

Biology Faculty Publications and Presentations

Livestock grazing, a widespread land use in semi-arid systems, is often placed in opposition to the perpetuation of biological soil crusts (“biocrusts”: lichens, mosses, and algal crusts including cyanobacteria) that live on the soil surface and provide ecosystem functions. The composition of biocrusts and vascular plants varies with climate, soils, and disturbance. In general, ruderal mosses and light algal crusts make up greater proportions of biocrusts in the presence of disturbance, although morphogroups of biocrusts respond differently to various disturbances. It is unknown if there are scenarios under which grazing can occur and ruderal components of biocrust could be maintained. …


A Phylogenetic Analysis Of The Population Dynamics Of A Captive Colony Of Diploptera Punctata., nick peterson 2024 University of Nebraska at Omaha

A Phylogenetic Analysis Of The Population Dynamics Of A Captive Colony Of Diploptera Punctata., Nick Peterson

Theses/Capstones/Creative Projects

The unique milk production of Diploptera punctata makes this species a promising model species in entomology for studying the evolution of milk production and for insect endocrinology. Despite this, the genetic diversity of captive populations of the species is not well studied. To better evaluate the captive diversity and population dynamics of Diploptera punctata at UNO we extracted the DNA of 20 random individuals and amplified their DNA using 4 different primers before cleaning the amplified products and sequencing the genes of the samples for use in both individual and concatenated phylogenetic trees. Our results indicate the UNO colony of …


Phenotypic Plasticity In Pigmentation: Investigating Coloration Patterns In Anablepsoides Hartii Population Responses To Environmental Variation, Jasdeep Kaur 2024 University of Texas at Arlington

Phenotypic Plasticity In Pigmentation: Investigating Coloration Patterns In Anablepsoides Hartii Population Responses To Environmental Variation, Jasdeep Kaur

2024 Spring Honors Capstone Projects

In this study, we investigated the color-changing capabilities of Trinidadian killifish, Anablepsoides hartii, through exposure to varied color conditions over defined periods of time. Our experiment aims to understand adaptive responses when killifish are subjected to different color backgrounds, exploring their mechanisms to change color. By subjecting the species to different environmental color variations, we sought to discern color change patterns, which could indicate adaptive responses related to camouflage, stress, behavior clues, and physiological adjustments. Carefully controlling environmental factors, we monitored and documented the fishes’ color changes, aiming to provide insights into the interplay between fish’s developmental plasticity and its …


Investigating And Developing Strategies For The Expression Of Biosynthetic Genes In Vitro And In Vivo, Tien Thuy Tran 2024 University of Tennessee, Knoxville

Investigating And Developing Strategies For The Expression Of Biosynthetic Genes In Vitro And In Vivo, Tien Thuy Tran

Doctoral Dissertations

Natural products have historically served as a rich source for a wide range of useful applications such aspesticides, veterinary agents, therapeutics, and bioproducts. To discover new natural products, manipulate them for analog generation, and harness the potential of these bioactive compounds for synthetic biology, it is necessary to develop robust methods for the expression of biosynthetic genes. A broad range of clinically useful natural products originate fromactinomycetes, especially those from the genus Streptomyces, which have been recognized as one of the predominant sources of microbial bioactive natural products. Actinobacteria are known for their large genomes, Guanine-Cytosine rich, and complex …


The Study Of Dmrt1 In Zebrafish And How It Impacts Sex Determination, Raymond Michael Poirier 2024 University of Massachusetts Boston

The Study Of Dmrt1 In Zebrafish And How It Impacts Sex Determination, Raymond Michael Poirier

Graduate Masters Theses

The dmrt1 gene is common amongst most animals and functions to determine or maintain male sex during development. Similarly, in zebrafish dmrt1 is important for male sex determination and maintaining proper testis morphology. This gene is expressed in two different cell types of the testis in zebrafish, germ cells and Sertoli cells. While we know where this gene is expressed and what its role is, it is not known if it is sufficient to drive male fate. If so, then in which cells is it sufficient to drive male fate in the testis? I aimed to answer this question by …


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