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Role Of Phosphoglucomutase Of Bordetella Bronchiseptica In Lipopolysaccharide Biosynthesis And Virulence, Nicholas P. West, Heidrun Jungnitz, John Fitter, Jason D. Mcarthur, Carlos Guzman, Mark J. Walker

Faculty of Science, Medicine and Health - Papers: part A

The phosphoglucomutase (PGM)-encoding gene of Bordetella bronchiseptica is required for lipopolysaccharide (LPS) biosynthesis. An insertion mutant of the wild-type B. bronchiseptica strain BB7865 which disrupted LPS biosynthesis was created and characterized (BB7865pgm). Genetic analysis of the mutated gene showed it shares high identity with PGM genes of various bacterial species and forms part of an operon which also encompasses the gene encoding phosphoglucose isomerase. Functional assays for PGM revealed that enzyme activity is expressed in bothbvg-positive and bvg-negative strains ofB. bronchiseptica and is substantially reduced in BB7865pgm. Complementation of the mutated PGM …