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Molecular Epidemiology Of Escherichia Coli 0157:H7 Strains By Bacteriophage A Restriction Fragment Length Polymorphism Analysis: Application To A Multistate Foodborne Outbreak And A Day-Care Center Cluster, Mansour Samadpour, Linda M. Grimm, B Desai, Dalia Alfi, Jerry E. Ongerth, Phillip I. Tarr

Faculty of Engineering and Information Sciences - Papers: Part A

Genomic DNAs prepared from 168 isolates of Escherichia coli 0157:H7 were analyzed for restriction fragment length polymorphisms on Southern blots probed with bacteriophage A DNA. The isolates analyzed included strains from a recent large multistate outbreak of E. coli 0157:H7 infection associated with consumption of poorly cooked beef in restaurants, a day-care center cluster, and temporally and geographically unrelated isolates. E. coli 0157:H7 isolates recovered from the incriminated meat and from 61 (96.8%) of 63 patients from Washington and Nevada possessed identical A restriction fragment length patterns. The A restriction fragment length polymorphisms observed in 11 (91.7%) of 12 day-care …