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Full-Text Articles in Social and Behavioral Sciences
Bovine Feces From Animals With Gastrointestinal Infections Are A Source Of Serologically Diverse Atypical Enteropathogenic Escherichia Coli And Shiga Toxin-Producing E. Coli Strains That Commonly Possess Intimin, Michael A Hornitzky, Kim Mercieca, Karl A Bettelheim, Steven P. Djordjevic
Bovine Feces From Animals With Gastrointestinal Infections Are A Source Of Serologically Diverse Atypical Enteropathogenic Escherichia Coli And Shiga Toxin-Producing E. Coli Strains That Commonly Possess Intimin, Michael A Hornitzky, Kim Mercieca, Karl A Bettelheim, Steven P. Djordjevic
Faculty of Science, Medicine and Health - Papers: part A
Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) cells were isolated from 191 fecal samples from cattle with gastrointestinal infections (diagnostic samples) collected in New South Wales, Australia. By using a multiplex PCR, E. coli cells possessing combinations of stx1, stx2, eae, and ehxA were detected by a combination of direct culture and enrichment in E. coli (EC) (modified) broth followed by plating on vancomycin-cefixime-cefsulodin blood (BVCC) agar for the presence of enterohemolytic colonies and on sorbitol MacConkey agar for the presence of non-sorbitol-fermenting colonies. The high prevalence of the intimin gene eae …
Bovine Non-O157 Shiga Toxin 2-Containing Escherichia Coli Isolates Commonly Possess Stx2-Edl933 And/Or Stx2vhb Subtypes, Kim Brett, Michael A Hornitzky, Karl A Bettelheim, Mark J. Walker, Steven Djordjevic
Bovine Non-O157 Shiga Toxin 2-Containing Escherichia Coli Isolates Commonly Possess Stx2-Edl933 And/Or Stx2vhb Subtypes, Kim Brett, Michael A Hornitzky, Karl A Bettelheim, Mark J. Walker, Steven Djordjevic
Faculty of Science, Medicine and Health - Papers: part A
stx2 genes from 138 Shiga toxin-producing Escherichia coli (STEC) isolates, of which 127 were of bovine origin (58 serotypes) and 11 of human origin (one serotype; O113:H21), were subtyped. The bovine STEC isolates from Australian cattle carried ehxA and/or eaeA and predominantly possessed stx2-EDL933 (103 of 127; 81.1%) either in combination with stx2vhb (32 of 127; 25.2%) or on its own (52 of 127; 40.4%). Of 22 (90.9%) bovine isolates of serotype O113:H21, a serotype increasingly recovered from patients with hemolytic uremic syndrome (HUS) or hemorrhagic colitis, 20 contained both stx2-EDL933 and stx2vhb; 2 …
Cell Surface Antigens Of Mycoplasma Species Bovine Group 7 Bind And Activate Plasminogen, Kylie Bower, Steven Djordjevic, Nicholas M Andronicos, Marie Ranson
Cell Surface Antigens Of Mycoplasma Species Bovine Group 7 Bind And Activate Plasminogen, Kylie Bower, Steven Djordjevic, Nicholas M Andronicos, Marie Ranson
Faculty of Science, Medicine and Health - Papers: part A
Mycoplasma species bovine group 7 bound plasminogen at the cell surface in a lysine-dependent manner. Cell-bound plasminogen was rapidly activated to plasmin by exogenous urokinase, and this activity was associated with plasminogen binding capacity. Binding assays using plasminogen modified with a trifunctional cross-linking agent revealed several binding proteins.