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Full-Text Articles in Physics
Effects Of Ether Vs. Ester Linkage On Lipid Bilayer Structure And Water Permeability., S. Deren Guler, D. Dipon Ghosh, Jianjun Pan, John Mathai, Mark Zeidel, John Nagle, Stephanie Tristram-Nagle
Effects Of Ether Vs. Ester Linkage On Lipid Bilayer Structure And Water Permeability., S. Deren Guler, D. Dipon Ghosh, Jianjun Pan, John Mathai, Mark Zeidel, John Nagle, Stephanie Tristram-Nagle
Prof. Stephanie Tristram-Nagle Ph.D.
The structure and water permeability of bilayers composed of the ether-linked lipid, dihexadecylphosphatidylcholine (DHPC), were studied and compared with the ester-linked lipid, dipalmitoylphosphaditdylcholine (DPPC). Wide angle X-ray scattering on oriented bilayers in the fluid phase indicate that the area per lipid A is slightly larger for DHPC than for DPPC. Low angle X-ray scattering yields A=65.1A(2) for DHPC at 48 degrees C. LAXS data provide the bending modulus, K(C)=4.2x10(-13)erg, and the Hamaker parameter H=7.2x10(-14)erg for the van der Waals attractive interaction between neighboring bilayers. For the low temperature phases with ordered hydrocarbon chains, we confirm the transition from a tilted …
Structure Of Lipid Bilayers, John Nagle, Stephanie Tristram-Nagle
Structure Of Lipid Bilayers, John Nagle, Stephanie Tristram-Nagle
Prof. Stephanie Tristram-Nagle Ph.D.
The quantitative experimental uncertainty in the structure of fully hydrated, biologically relevant, fluid (L(alpha)) phase lipid bilayers has been too large to provide a firm base for applications or for comparison with simulations. Many structural methods are reviewed including modern liquid crystallography of lipid bilayers that deals with the fully developed undulation fluctuations that occur in the L(alpha) phase. These fluctuations degrade the higher order diffraction data in a way that, if unrecognized, leads to erroneous conclusions regarding bilayer structure. Diffraction measurements at high instrumental resolution provide a measure of these fluctuations. In addition to providing better structural determination, this …
Orientation Of Tie-Lines In The Phase Diagram Of Dopc/Dppc/Cholesterol Model Biomembranes., Pradeep Uppamoochikkal, Stephanie Tristram-Nagle, John Nagle
Orientation Of Tie-Lines In The Phase Diagram Of Dopc/Dppc/Cholesterol Model Biomembranes., Pradeep Uppamoochikkal, Stephanie Tristram-Nagle, John Nagle
Prof. Stephanie Tristram-Nagle Ph.D.
We report the direction of tie-lines of coexisting phases in a ternary diagram of DOPC/DPPC/cholesterol lipid bilayers, which has been a system of interest in the discussion of biological rafts. For coexisting Ld and Lo phases, we find that the orientation angle α of the tie-lines increases as the cholesterol concentration increases and it also increases as temperature increases from T = 15 °C to T = 30 °C. Results at lower cholesterol concentrations support the existence of a different two-phase coexistence region of Ld and So phases and the existence of a three-phase region separating the two two-phase regions. …
Volumetric Stability Of Lipid Bilayers., Kelsey Hallinen, Stephanie Tristram-Nagle, John Nagle
Volumetric Stability Of Lipid Bilayers., Kelsey Hallinen, Stephanie Tristram-Nagle, John Nagle
Prof. Stephanie Tristram-Nagle Ph.D.
In agreement with recent reports, a commercial densimeter has yielded a gradual decrease in lipid molecular volume of DPPC multilamellar vesicle dispersions in the gel phase upon repeated thermal cycling between 10 °C and 50 °C. The considerable size of this decrease would have significant implications for the physical chemistry of biomembranes. In contrast, neutral buoyancy measurements performed with similar thermal cycling indicate no gradual change in lipid volume in the gel phase at 20 °C. Remixing the lipid in the densimeter shows that the apparent volume decrease is an artifact. We conclude that gel phase DPPC bilayers exist in …