Physical Sciences and Mathematics Commons^™

Sensitivity Of The Relationship Between Antarctic Ice Shelves And Iron Supply To Projected Changes In The Atmospheric Forcing, Michael S. Dinniman, Pierre St-Laurent, Kevin R. Arrigo, Eileen E. Hofmann, Gert L. Van Dijken

CCPO Publications

Upward advection or mixing of iron-rich deep waters due to circulation changes driven by the rate of basal ice shelf melt was shown to be a primary control on chlorophyll a production in coastal polynyas over the Antarctic continental shelf. Here, the effects of atmospheric changes projected in 2100 on this relationship were examined with a 5-km resolution ocean/sea ice/ice shelf model of the Southern Ocean with different simulated dissolved iron sources and idealized biological uptake. The atmospheric changes are added as idealized increments to the forcing. Inclusion of a poleward shift and strengthening of the winds, increased precipitation, and …

Contrasting Sea-Ice Algae Blooms In A Changing Arctic Documented By Autonomous Drifting Buoys, Victoria Hill, Bonnie Light, Michael Steele, Andrew Lowy Sybrandy

OES Faculty Publications

Novel observations of the seasonal evolution of an ice algal bloom on the Chukchi shelf were collected by two autonomous buoys deployed 180 km apart in first-year drifting sea ice. High attenuation of blue light in the bottom of the ice indicated considerable accumulation of ice algae biomass with derived Chlorophyll-a concentrations (Chl a) up to 184 mg m⁻². Differences in the magnitude and persistence of ice algae biomass under each buoy appear to have been driven by differences in snow thickness, as ice thickness was similar between the sites. Minimal snow cover (0.02 m) around …

Photorespiration In Eelgrass (Zostera Marina L.): A Photoprotection Mechanism For Survival In A Co₂-Limited World, Billur Celebi-Ergin, Richard C. Zimmerman, Victoria J. Hill

OES Faculty Publications

Photorespiration, commonly viewed as a loss in photosynthetic productivity of C3 plants, is expected to decline with increasing atmospheric CO₂, even though photorespiration plays an important role in the oxidative stress responses. This study aimed to quantify the role of photorespiration and alternative photoprotection mechanisms in Zostera marina L. (eelgrass), a carbon-limited marine C3 plant, in response to ocean acidification. Plants were grown in controlled outdoor aquaria at different [CO₂]aq ranging from ~55 (ambient) to ~2121 μM for 13 months and compared for differences in leaf photochemistry by simultaneous measurements of O₂ flux and …

Skeletal Light-Scattering Accelerates Bleaching Response In Reef-Building Corals, Timothy D. Swain, Emily Dubois, Andrew Gomes, Valentina P. Stoyneva, Andrew J. Radosevich, Jillian Henss, Michelle E. Wagner, Justin Derbas, Hannah Grooms, Elizabeth M. Velazquez, Joshua Traub, Brian J. Kennedy, Arabela A. Grigorescu, Mark W. Westneat, Kevin Sanborn, Shoshana Levine, Mark Schick, George Parsons, Brendan C. Briggs, Jeremy D. Rogers, Vadim Backman, Luisa A. Marcelino

Timothy Swain

Background At the forefront of ecosystems adversely affected by climate change, coral reefs are sensitive to anomalously high temperatures which disassociate (bleaching) photosynthetic symbionts (Symbiodinium) from coral hosts and cause increasingly frequent and severe mass mortality events. Susceptibility to bleaching and mortality is variable among corals, and is determined by unknown proportions of environmental history and the synergy of Symbiodinium- and coral-specific properties. Symbiodinium live within host tissues overlaying the coral skeleton, which increases light availability through multiple light-scattering, forming one of the most efficient biological collectors of solar radiation. Light-transport in the upper ~200 μm layer …

Ebullition Of Oxygen From Seagrasses Under Supersaturated Conditions, Matthew H. Long, Kevin Sutherland, Scott D. Wankel, David J. Burdige, Richard C. Zimmerman

OES Faculty Publications

Gas ebullition from aquatic systems to the atmosphere represents a potentially important fraction of primary production that goes unquantified by measurements of dissolved gas concentrations. Although gas ebullition from photosynthetic surfaces has often been observed, it is rarely quantified. The resulting underestimation of photosynthetic activity may significantly bias the determination of ecosystem trophic status and estimated rates of biogeochemical cycling from in situ measures of dissolved oxygen. Here, we quantified gas ebullition rates in Zostera marina meadows in Virginia, U.S.A. using simple funnel traps and analyzed the oxygen concentration and isotopic composition of the captured gas. Maximum hourly rates of …

Experimental Impacts Of Climate Warming And Ocean Carbonation On Eelgrass Zostera Marina, Richard C. Zimmerman, Victoria J. Hill, Malee Jinuntuya, Billur Celebi, David Ruble, Miranda Smith, Tiffany Cedeno, W. Mark Swingle

OES Faculty Publications

CO₂ is a critical and potentially limiting substrate for photosynthesis of both terrestrial and aquatic ecosystems. In addition to being a climate-warming greenhouse gas, increasing concentrations of CO₂ will dissolve in the oceans, eliciting both negative and positive responses among organisms in a process commonly known as ocean acidification. The dissolution of CO₂ into ocean surface waters, however, also increases its availability for photosynthesis, to which the highly successful, and ecologically important, seagrasses respond positively. Thus, the process might be more accurately characterized as ocean carbonation. This experiment demonstrated that CO₂ stimulation of primary production enhances …

Community Production Modulates Coral Reef Ph And The Sensitivity Of Ecosystem Calcification To Ocean Acidification, Thomas M. Decarlo, Anne L. Cohen, George T. F. Wong, Fuh-Kwo Shiah, Steven J. Lentz, Kristen A. Davis, Kathryn E. F. Shamberger, Pat Lohmann

OES Faculty Publications

Coral reefs are built of calcium carbonate (CaCO₃) produced biogenically by a diversity of calcifying plants, animals, and microbes. As the ocean warms and acidifies, there is mounting concern that declining calcification rates could shift coral reef CaCO3 budgets from net accretion to net dissolution. We quantified net ecosystem calcification (NEC) and production (NEP) on Dongsha Atoll, northern South China Sea, over a 2 week period that included a transient bleaching event. Peak daytime pH on the wide, shallow reef flat during the nonbleaching period was ~8.5, significantly elevated above that of the surrounding open ocean (~8.0-8.1) as …

Potential Impacts Of Climate Change On Photochemistry Of Zostera Marina L., Billur Celebi

OES Theses and Dissertations

Seagrasses account for approximately 10% of the ocean’s total carbon storage, although photosynthesis of seagrasses is carbon limited at today’s oceanic pH. Therefore, increasing atmospheric CO₂ concentration, which results in ocean acidification/carbonation, is predicted to have a positive impact on seagrass productivity. Previous studies have confirmed the positive influence of increasing CO₂ on photosynthesis and survival of the temperate eelgrass Zostera marina L., but the acclimation of photoprotective mechanisms in this context has not been characterized. This study aimed to quantify the long-term impacts of ocean acidification on photochemical control mechanisms that promote photosynthesis while simultaneously protecting …

Skeletal Light-Scattering Accelerates Bleaching Response In Reef-Building Corals, Timothy D. Swain, Emily Dubois, Andrew Gomes, Valentina P. Stoyneva, Andrew J. Radosevich, Jillian Henss, Michelle E. Wagner, Justin Derbas, Hannah Grooms, Elizabeth M. Velazquez, Joshua Traub, Brian J. Kennedy, Arabela A. Grigorescu, Mark W. Westneat, Kevin Sanborn, Shoshana Levine, Mark Schick, George Parsons, Brendan C. Briggs, Jeremy D. Rogers, Vadim Backman, Luisa A. Marcelino

Marine & Environmental Sciences Faculty Articles

Background At the forefront of ecosystems adversely affected by climate change, coral reefs are sensitive to anomalously high temperatures which disassociate (bleaching) photosynthetic symbionts (Symbiodinium) from coral hosts and cause increasingly frequent and severe mass mortality events. Susceptibility to bleaching and mortality is variable among corals, and is determined by unknown proportions of environmental history and the synergy of Symbiodinium- and coral-specific properties. Symbiodinium live within host tissues overlaying the coral skeleton, which increases light availability through multiple light-scattering, forming one of the most efficient biological collectors of solar radiation. Light-transport in the upper ~200 μm layer …

Effects Of Elevated Pco2 And Irradiance On Growth, Photosynthesis And Calcification In Halimeda Discoidea, K. E. Peach, M. S. Koch, Patricia Blackwelder

Marine & Environmental Sciences Faculty Articles

Ocean acidification (OA) effects on photophysiology and calcification were examined in Halimeda discoidea, a calcifying macroalga that produces tropical reef sediments. Photosynthetic parameters, including maximum photosynthetic rate (P_max), photosynthetic efficiency (α) and compensating irradiance (I_c) were determined in short-term assays on live thalli after a 10 d exposure to 4 levels of CO₂ partial pressures (pCO₂; 491, 653, 982 and 1201 µatm) under saturating (300 µmol photons m^-2 s^-1) and sub-saturating (90 µmol photons m^-2 s^-1) irradiance in an aquaria study. Morphology …

Productivity, Absorbed Photosynthetically Active Radiation, And Light Use Efficiency In Crops: Implications For Remote Sensing Of Crop Primary Production, Anatoly A. Gitelson, Yi Peng, Timothy J. Arkebauer, Andrew E. Suyker

School of Natural Resources: Faculty Publications

Vegetation productivity metrics such as gross primary production (GPP) at the canopy scale are greatly affected by the efficiency of using absorbed radiation for photosynthesis, or light use efficiency (LUE). Thus, close investigation of the relationships between canopy GPP and photosynthetically active radiation absorbed by vegetation is the basis for quantification of LUE. We used multiyear observations over irrigated and rainfed contrasting C3 (soybean) and C4 (maize) crops having different physiology, leaf structure, and canopy architecture to establish the relationships between canopy GPP and radiation absorbed by vegetation and quantify LUE. Although multiple LUE definitions are reported in the literature, …

Divergent Responses Of Atlantic Coastal And Oceanic Synechococcus To Iron Limitation, Katherine R. M. Mackey, Anton F. Post, Matthew R. Mcilvin, Gregory A. Cutter, Seth G. John, Mak A. Saito

OES Faculty Publications

Marine Synechococcus are some of the most diverse and ubiquitous phytoplankton, and iron (Fe) is an essential micronutrient that limits productivity in many parts of the ocean. To investigate how coastal and oceanic Atlantic Synechococcus strains acclimate to Fe availability, we compared the growth, photophysiology, and quantitative proteomics of two Synechococcus strains from different Fe regimes. Synechococcus strain WH8102, from a region in the southern Sargasso Sea that receives substantial dust deposition, showed impaired growth and photophysiology as Fe declined, yet used few acclimation responses. Coastal WH8020, from the dynamic, seasonally variable New England shelf, displayed a multitiered, hierarchical cascade …

Understanding Ocean Acidification Impacts On Organismal To Ecological Scales, Andreas J. Andersson, David I. Kline, Peter J. Edmunds, Stephen D. Archer, Nina Bednarsek, Robert C. Carpenter, Meg Chadsey, Philip Goldstein, Andrea G. Grottoli, Thomas P. Hurst, Andrew L. King, Janet E. Kübler, Ilsa B. Kuffner, Katherine R.M. Mackey, Bruce A. Menge, Adina Paytan, Ulf Riebesell, Astrid Schnetzer, Mark E. Warner, Richard C. Zimmerman

OES Faculty Publications

Ocean acidification (OA) research seeks to understand how marine ecosystems and global elemental cycles will respond to changes in seawater carbonate chemistry in combination with other environmental perturbations such as warming, eutrophication, and deoxygenation. Here, we discuss the effectiveness and limitations of current research approaches used to address this goal. A diverse combination of approaches is essential to decipher the consequences of OA to marine organisms, communities, and ecosystems. Consequently, the benefits and limitations of each approach must be considered carefully. Major research challenges involve experimentally addressing the effects of OA in the context of large natural variability in seawater …

Predicting Carbon Isotope Discrimination In Eelgrass (Zostera Marina L.) From The Environmental Parameters- Light, Flow, And [Dic], Meredith L. Mcpherson, Richard C. Zimmerman, Victoria J. Hill

OES Faculty Publications

Isotopic discrimination against ¹³C during photosynthesis is determined by a combination of environmental conditions and physiological mechanisms that control delivery of CO₂ to RUBISCO. This study investigated the effects of light, flow, dissolved inorganic carbon (DIC) concentration, and its speciation, on photosynthetic carbon assimilation of Zostera marinaL. (eelgrass) using a combination of laboratory experiments and theoretical calculations leading to a mechanistic understanding of environmental conditions that influence leaf carbon uptake and determine leaf stable carbon isotope signatures δ¹³C. Photosynthesis was saturated with respect to flow at low velocity ~ 3 cm s^-1, but …

Chlorophyll Fluorescence And Thermal Stress In Archaias Angulatus (Class Foraminifera), Heidi M. Toomey

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Benthic foraminifers that host algal symbionts are similar to corals in that they rely on their algal endosymbionts for their energy needs, calcify prolifically, and are sensitive to changes in environmental conditions. They are abundant in the benthos of coastal coral-reef areas and are found throughout the tropical and subtropical regions. Pulse Amplitude Modulated (PAM) chlorophyll fluorometry and chlorophyll a extraction techniques were used to quantify and compare the photosynthetic responses of the benthic foraminiferal, Archaias angulatus and their isolated endosymbionts, Chlamydomonas hedleyi, to short-term changes in temperature. Maximum quantum efficiency (F_v/F_m) and rapid …

Distributions Of Particulate Heme B In The Atlantic And Southern Oceans- Implications For Electron Transport In Phytoplankton, Martha Gledhill, Eric P. Achterberg, David J. Honey, Maria C. Nielsdottir, Micha J.A. Rijkenberg

OES Faculty Publications

Concentrations of heme b, the iron-containing component of b-type hemoproteins, ranged from b concentrations were enhanced in the photic zone and decreased with depth. Heme b concentrations correlated positively with chlorophyll a (chl a) in the TNA (r=0.41, pb did not correlate with chl a in the IB or SS. In the IB and SS, stations with high-chlorophyll and low-nutrient (Fe and/or Si) concentrations exhibited low heme bconcentrations relative to particulate organic carbon (< 0.1 μmolmol^-1, and high chl a:heme b ratios (> 500). High chl a:heme b ratios resulted from relative decreases in …

Phosphorus Cycling In The Sargasso Sea: Investigation Using The Oxygen Isotopic Composition Of Phosphate, Enzyme-Labeled Fluorescence, And Turnover Times, Karen Mclaughlin, Jill A. Sohm, Gregory A. Cutter, Michael W. Lomas, Adina Paytan

OES Faculty Publications

Dissolved inorganic phosphorus (DIP) concentrations in surface water of vast areas of the ocean are extremely low (<10 nM) and phosphorus (P) availability could limit primary productivity in these regions. We explore the use of oxygen isotopic signature of dissolved phosphate (δ¹⁸O_PO4) to investigate biogeochemical cycling of P in the Sargasso Sea, Atlantic Ocean. Additional techniques for studying P dynamics including ³³P-based DIP turnover time estimates and percent of cells expressing alkaline phosphatase (AP) activity as measured by enzyme-labeling fluorescence are also used. In surface waters, δ¹⁸O_PO4 values were lower than equilibrium by 3–6%, indicative of dissolved organic phosphorous (DOP) remineralization by extracellular enzymes. An isotope mass balance model using a variety of possible combinations of …

Δ13c Is A Signature Of Light Availability And Photosynthesis In Seagrass, Xingping Hu, David J. Burdige, Richard C. Zimmerman

OES Faculty Publications

We explored the role of light-saturated (carbon-limited) photosynthesis on δ¹³C of turtlegrass (Thalassia testudinum Banks ex Konig) populations from the clear, blue waters of the Great Bahama Bank and the turbid, green waters of Florida Bay using field observations and radiative transfer models. Consistent with numerous previous observations, leaf δ¹³C decreased significantly with water depth in both regions. However the δ¹³C for Bahamas turtlegrass was 3 parts per thousand heavier than that for Florida Bay turtlegrass at equivalent depths, and broadband irradiance explained even less of the δ¹³C variations than depth. Instead, leaf δ¹³C showed a stronger relationship to …

Modeling The Vertical Distributions Of Downwelling Plane Irradiance And Diffuse Attenuation Coefficient In Optically Deep Waters, X. J. Pan, Richard C. Zimmerman

OES Faculty Publications

The diffuse attenuation coefficient Kᵈ is critical to understand the vertical distribution of underwater downwelling irradiance (Eᵈ). Theoretically Eᵈ is composed of the direct solar beam and the diffuse sky irradiance. Applying the statistical results from Hydrolight radiative transfer simulations, Kᵈ is expressed into a mathematical equation (named as PZ06) integrated from the contribution of direct solar beam and diffuse sky irradiance with the knowledge of sky and water conditions. The percent root mean square errors (RMSE) for the vertical distribution of Eᵈ(z) under various sky and water conditions between PZ06 and Hydrolight results are typically less than 4%. Field …

Simulations Of Phytoplankton Species And Carbon Production In The Equatorial Pacific Ocean 1. Model Configuration And Ecosystem Dynamics, Baris Salihoglu, Eileen E. Hofmann

OES Faculty Publications

The primary objective of this research is to investigate phytoplankton community response to variations in physical forcing and biological processes in the Cold Tongue region of the equatorial Pacific Ocean at 0N, 140W. This research objective was addressed using a one-dimensional multicomponent lower trophic level ecosystem model that includes detailed algal physiology, such as spectrally-dependent photosynthetic processes and iron limitation on algal growth. The ecosystem model is forced by a one-year (1992) time series of spectrally-dependent light, temperature, and water column mixing obtained from a Tropical Atmosphere-Ocean (TAO) Array mooring. Autotrophic growth is represented by five algal groups, which have …

Dynamics Of Carbon Allocation In A Deep-Water Population Of The Deciduous Kelp Pleurophycus Gardeneri (Laminariales), Clare M. Dominik, Richard C. Zimmerman

OES Faculty Publications

Pleurophycus gardneri (Laminariales) is common in the low intertidal of the Northeast Pacific, but dominates many deep (30 to 40 m) rocky reefs in central California. Seasonal dynamics of productivity and resource allocation of a deep-water population of this deciduous, stipitate kelp were studied to understand how blade abscission affects the annual carbon budget. Patterns of growth, metabolism, and carbon storage and mobilization were measured monthly for 1 yr relative to in situ light and temperature, and used to model the annual carbon budget. The resulting carbon budget was used to determine if blade abscission effectively reduced respiratory demand during …

A Nonparametric Method For Separating Photosynthesis And Respiration Components In Co2 Flux Measurements, Chuixiang Yi, Runze Li, Peter S. Backwin, Ankur Desai, Daniel M. Ricciuto, Sean P. Burns, Andrew A. Turnipseed, Steven C. Wofsy, J. William Munger, Kell Wilson, Russell K. Monson

Publications and Research

Future climate change is expected to affect ecosystem-atmosphere CO₂ exchange, particularly through the influence of temperature. To date, however, few studies have shown that differences in the response of net ecosystem CO₂ exchange (NEE) to temperature among ecosystems can be explained by differences in the photosynthetic and respiratory processes that compose NEE. Using a new nonparametric statistical model, we analyzed data from four forest ecosystems. We observed that differences among forests in their ability to assimilate CO₂ as a function of temperature were attributable to consistent differences in the temperature dependence of photosynthesis and respiration. This observation …

Impact Of Sea Grass Density On Carbonate Dissolution In Bahamian Sediments, David J. Burdige, Richard C. Zimmerman

OES Faculty Publications

Carbonate dissolution has been widely observed in shallow water tropical sediments. However, sediment budgets C have generally not been closed with respect to the amount of acid required to produce the observed carbonate dissolution. Recently it has been suggested that enhanced oxygen transport into sediments through the roots and rhizomes of sea grasses might play a role in resolving this mass balance problem. We conducted studies of sea grass-carbonate sediment interactions around Lee Stocking Island, Exuma Islands, Bahamas to further examine this problem. Our studies showed that alkalinity, total dissolved inorganic carbon (ΣCO₂) and Ca²⁺ increased with …

Top-Down Impact Through A Bottom-Up Mechanism. In Situ Effects Of Limpet Grazing On Growth, Light Requirements And Survival Of The Eelgrass Zostera Marina, Richard C. Zimmerman, Diana L. Steller, Donald G. Kohrs, Randall S. Alberte

OES Faculty Publications

Temporal changes in abundance, size, productivity, resource allocation and light requirements of a subtidal eelgrass (Zostera marina L.) population were followed for 2 yr after the September 1993 appearance of a previously rare oval form of the commensal limpet Tectura depicta (Berry) in Monterey Bay, California, USA, By exclusively targeting the epidermis, limpet grazing impaired photosynthetic performance but left respiratory demand, meristematic growth and more than 90 % of the leaf biomass intact, The resulting low P:R ratios of grazed plants raised the light requirements for the maintenance of positive carbon balance almost 2-fold relative to healthy ungrazed plants …

Resource Allocation And Sucrose Mobilization In Light Limited Eelgrass Zostera Marina, Teresa Alcoverro, Richard C. Zimmerman, Donald G. Kohrs, Randall S. Alberte

OES Faculty Publications

This study evaluated the ability of Zostera marina L. (eelgrass) to balance the daily photosynthetic deficit by mobilization of carbon reserves stored in below-ground tissues during a period of extreme winter light limitation. A quantitative understanding of the mobilization process and its limitations is essential to the development of robust models predicting minimum light levels required to maintain healthy seagrass populations. Plants were grown in running seawater tanks under 2 light regimes. One treatment was provided with 2 h irradiance-saturated photosynthesis (H_sat) to produce severe Light Limitation, while control plants were grown under 7 h H_sat, …

Effect Of Light/Dark Transition On Carbon Translocation In Eelgrass Zostera Marina Seedlings, Richard C. Zimmerman, Randall S. Alberte

OES Faculty Publications

Carbon translocation in the marine macrophyte Zostera marina L. (eelgrass) was investigated to elucidate the impact of light/dark transitions on sucrose partitioning between roots and shoots. After exposure of leaves to C-14-bicarbonate, the level of C-14-labelled photoassimilates increased monotonically in both leaves and fully aerobic roots of plants maintained in the light. Accumulation of C-14 in roots and leaves ceased abruptly when plants were transferred to darkness that induced root anaerobiosis even though C-14 levels remained high in the dark-exposed leaves. Thus, translocation of C-14 photoassimilates from shoots to roots was inhibited when roots became anoxic. Anoxia induced by light …

Modeling Daily Production Of Aquatic Macrophytes From Irradiance Measurements: A Comparative Analysis, Richard C. Zimmerman, Alejandro C. Pasini, Randall S. Alberte

OES Faculty Publications

The importance of submerged aquatic macrophytes to coastal ecosystems has generated a need for knowledge of minimum light levels that will support the maintenance and restoration of healthy populations. Our goals were (1) to evaluate the sensitivity to natural, non-sinusoidal fluctuations in irradiance I of analytical integration techniques for calculating daily carbon gain, (2) to evaluate the H_sat (the daily period of I-saturated photosynthesis) model of daily production relative to models based on instantaneous photosynthesis vs irradiance (P vs I) and (3) to provide some guidance for the temporal density of irradiance data required for accurate estimation of …

The Photosynthetic Action Spectra Of The Phytoplankton And Their Role In Governing Spatial And Temporal Distribution: A Numerical Modeling Approach, William Morrow Willis

OES Theses and Dissertations

In the present study a numerical modeling approach is employed to examine the role of division spedific differences in photosynthetic action spectra in governing the relative size of diatom and dinoflagellate carbon synthesis along various marine light regime gradients. A radiative transfer model taking into account both Rayleigh and Mie atmospheric optical properties is employed to define the light regime incident on the sea surface. The hydrospheric light regime is defined by an exponential decay model with a correction for diffuse back scatter. Taken together, the atmospheric and hydrospheric models define the spectral composition and intensity of light in the …

Seasonal And Spatial Variation In Primary Productivity In Boulder Basin, Lake Mead, Clark County, Nevada, Isamu Aoki

Publications (WR)

The ¹⁴C light and dark bottle technique for measurement of primary production was utilized as a means of assessing the amount of inorganic carbon being converted Into organic form by the photosynthesis of phytoplankton populations In the Boulder Basin of Lake Mead.

Spatial and time series changes of productivity levels observed at eight sampling locations within Boulder Basin Indicate that the Influence of treated municipal arts' industrial effluent flowing into Les Vegas Bay is contributing high levels of available nutrients at Las Vegas Wash Inlet to cause productivity to approximate those levels associated with polluted waters.

Productivity levels at …