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Full-Text Articles in Physical Sciences and Mathematics
Age-Dependent Increase In Ortho-Tyrosine And Methionine Sulfoxide In Human Skin Collagen Is Not Accelerated In Diabetes: Evidence Against A Generalized Increase In Oxidative Stress In Diabetes, Mary C. Wells-Knecht, Timothy J. Lyons, David R. Mccance, Suzanne R. Thorpe, John W. Baynes
Age-Dependent Increase In Ortho-Tyrosine And Methionine Sulfoxide In Human Skin Collagen Is Not Accelerated In Diabetes: Evidence Against A Generalized Increase In Oxidative Stress In Diabetes, Mary C. Wells-Knecht, Timothy J. Lyons, David R. Mccance, Suzanne R. Thorpe, John W. Baynes
Faculty Publications
The glycoxidation products Nepsilon-(carboxymethyl)lysine and pentosidine increase in skin collagen with age and at an accelerated rate in diabetes. Their age-adjusted concentrations in skin collagen are correlated with the severity of diabetic complications. To determine the relative roles of increased glycation and/or oxidation in the accelerated formation of glycoxidation products in diabetes, we measured levels of amino acid oxidation products, distinct from glycoxidative modifications of amino acids, as independent indicators of oxidative stress and damage to collagen in aging and diabetes. We show that ortho-tyrosine and methionine sulfoxide are formed in concert with Nepsilon-(carboxymethyl)lysine and pentosidine during glycoxidation of collagen …
Carboxymethylethanolamine, A Biomarker Of Phospholipid Modification During The Maillard Reaction In Vivo, Jesus R. Requena, Mahtab U. Ahmed, C. Wesley Fountain, Thorsten P. Degenhardt, Sharanya Reddy, Cliff Perez, Timothy J. Lyons, Alicia J. Jenkins, John W. Baynes, Suzanne R. Thorpe
Carboxymethylethanolamine, A Biomarker Of Phospholipid Modification During The Maillard Reaction In Vivo, Jesus R. Requena, Mahtab U. Ahmed, C. Wesley Fountain, Thorsten P. Degenhardt, Sharanya Reddy, Cliff Perez, Timothy J. Lyons, Alicia J. Jenkins, John W. Baynes, Suzanne R. Thorpe
Faculty Publications
Nepsilon-(Carboxymethyl)lysine (CML) is a stable chemical modification of proteins formed from both carbohydrates and lipids during autoxidation reactions. We hypothesized that carboxymethyl lipids such as (carboxymethyl)phosphatidylethanolamine (carboxymethyl-PE) would also be formed in these reactions, and we therefore developed a gas chromatography-mass spectrometry assay for quantification of carboxymethylethanolamine (CME) following hydrolysis of phospholipids. In vitro, CME was formed during glycation of dioleoyl-PE under air and from linoleoylpalmitoyl-PE, but not from dioleoyl-PE, in the absence of glucose. In vivo, CME was detected in lipid extracts of red blood cell membranes, approximately 0.14 mmol of CME/mol of ethanolamine, from control and diabetic subjects, …
Quantitation Of Ethidium-Stained Closed Circular Dna In Agarose Gels, Michael F. Shubsda, Jerry Goodisman, James C. Dabrowiak
Quantitation Of Ethidium-Stained Closed Circular Dna In Agarose Gels, Michael F. Shubsda, Jerry Goodisman, James C. Dabrowiak
Chemistry - All Scholarship
The fluorescence of ethidium bromide (EB) bound to equimolar amounts of supercoiled form I and unstrained linear form III pBR322, SV40 and PM2 DNA in agarose gels has been measured by scanning a photographic negative of the gel with a microdensitometer. For SV40 and PM2 DNA, commonly used staining conditions cause both forms, i.e. linear and supercoiled, to fluoresce to the same extent. This obviates the need to use a correction factor for the fluorescence of form I DNA when measuring the amount of this form relative to the amounts of unstrained forms in agarose gels. In the case of …
The Resolution Of Chiral Dialkylarylsufonium Ions By 1h Nmr Spectroscopy, Johnathan Whetstine
The Resolution Of Chiral Dialkylarylsufonium Ions By 1h Nmr Spectroscopy, Johnathan Whetstine
Mahurin Honors College Capstone Experience/Thesis Projects
The goal of this research was to design a rapid Nuclear Magnetic Resonance method to measure enantiomeric excess of chiral sulfonium ions by using chiral lanthanide shift reagents. The sulfonium ions studied were dialkylarylsulfonium ions. The shift reagent that was employed was Europium (III) surrounded by chiral ligands of 3-trifluoroacetyl-d-camphor (tfc), which is derived from (+)-camphor. Ethylmcthylphenylsulfonium ion was synthesized for study as a racemic and enantiomerically enriched mixture. The sulfonium ion was then ion-paired with the shift reagent for resolution of R and S enantiomers. The best resolution was obtained with the Eu(fod)4- shift reagent, with nearly baseline resolution …
Efficient And General Synthesis Of Novel Β-Polyfluoroalkoxy Vinamidinium Salts, Koichiro Kase, Mitsuyoshi Katayama, Takashi Ishihara, Hiroki Yamanaka, John T. Gupton
Efficient And General Synthesis Of Novel Β-Polyfluoroalkoxy Vinamidinium Salts, Koichiro Kase, Mitsuyoshi Katayama, Takashi Ishihara, Hiroki Yamanaka, John T. Gupton
Chemistry Faculty Publications
Novel β-polyfluoroalkoxy vinamidinium salts 3 and/or 4 were synthesized in good yields by the reaction of N-(2- polyfluoroalkoxy-3 ,3-difluoro-1-propenyI)trimethylammonium iodides (2), prepared from N-(2,3,3-trifluoro-1-propenyl)trimethylammonium iodide (1), with secondary amines in MeCN at 70 °C for 1 h. The salts were also obtainable in comparable yields by the one-pot reaction of 1 with sodium polyfluoroalkoxide followed by treatment with amines.
Kinetics Of Cleavage Of Intra- And Extracellular Simian Virus 40 Dna With The Enediyne Anticancer Drug C- 1027, Jerry Goodisman, James C. Dabrowiak, Christopher A. Kirk, Terry A. Beerman, Loretta S. Gawron
Kinetics Of Cleavage Of Intra- And Extracellular Simian Virus 40 Dna With The Enediyne Anticancer Drug C- 1027, Jerry Goodisman, James C. Dabrowiak, Christopher A. Kirk, Terry A. Beerman, Loretta S. Gawron
Chemistry - All Scholarship
A kinetic analysis of cleavage of simian virus DNA (SV40 DNA) inside and outside green monkey BSC-I cells by the enediyne-protein antibiotic C-1027 and its free chromophore is described. Information on rate constants was obtained by fitting populations of forms I (closed circular DNA), II (nicked circular DNA) and III (linear DNA) of SV4f.J DNA as a function of drug concentration to a kinetic model which includes: cutting of form I to give form II with rate constant k1. cutting of form I to give form III with rate constant k4, and cutting of form II to give form III …
Solvent Impregnated Resins For The Recovery Of Gold From Gold(I)Thiourea Solutions, Fiona Fitzpatrick
Solvent Impregnated Resins For The Recovery Of Gold From Gold(I)Thiourea Solutions, Fiona Fitzpatrick
Masters
Solvent Impregnated Resins (SIRs) are high surface area, macroporous, polymeric resins which have been physically impregnated with an organic solvent. In this research, three resins have been impregnated with Di-2-ethylhexyl phosphoric acid, a solvent which has shown selectivity to gold. The ability of the resins to recover gold (1) thiourea perchlorate from solution has been investigated. The resins have been studied both bare and when impregnated with the solvent and their efficiency was seen to increase upon impregnation. The overall efficiency of the resins was found to be pH dependent. It is also related to the level of impregnation of …