Physical Sciences and Mathematics Commons^™

Chemical And Genetic Composition Analysis Of Organic And Nonorganic Tortilla Chips, Aubrey White-Day

Honors Theses

The aim of this study was to explore the chemical and genetic differences between organic and nonorganic tortilla chips using GC-MS and PCR. Twenty chip brands were selected: 10 organic and 10 nonorganic. A survey on shopping preferences was created and distributed to compare results of public opinion to experimental data. It yielded 212 responses. All organic chip brands, and one out of ten nonorganic chip brands, tested negative for GMOs. This study concluded that there are minimal chemical differences based on Jaccard similarity indicies and stark genetic differences between organic and nonorganic tortilla chips. In comparing statistical analyses to …

Copper-Indazolate Nanojars: Synthesis And Characterization, Nicholas Alexander Rudell

Masters Theses

The field of synthetic supramolecular chemistry has exploded since the first syntheses of crown ethers by Charles Pedersen in the 1960s, leading to further development in the area of macrocycles and incorporation into a vast array of metal-organic structures. Supramolecular chemistry came to the forefront of science with the 1987 Nobel Prize in Chemistry, awarded jointly to Donald J. Cram, Jean-Marie Lehn, and Charles Pedersen “for their development and use of molecules with structure-specific interactions of high selectivity.”

Nanojars are supramolecular metal-organic complexes with a high affinity for hydrophilic anions and can efficiently transfer such anions from water into organic …

Characterization Of Shinella 6-Hydroxy-(L)-Nicotine Oxidase (6hlno-Se), Kaitrin Funckes

Honors Theses

6-hydroxy-(L)-nicotine oxidase, isolated from the organism Shinella sp. HZN7 (6HLNO-SE), is an enzyme that during the catabolism of nicotine catalyzes the oxidation of (S)-6-hydroxynicotine to 6-hydroxypseudeooxynicotine. This enzyme is a member of the monoamine oxidase (MAO) structural family of flavoproteins, which contain a highly conserved FAD-binding domain. Within the binding domain is a conserved lysine residue that can be traced throughout evolutionary history in this family. 6HLNO isolated from Arthrobacter nicotinovorans serves as a model enzyme for the one isolated from Shinella as both catalyze the same reaction in this biochemical pathway and much has been studied about the Arthrobacter …

Kinetic And Structural Characterization Of Pseudooxynicotine Amine Oxidase From Pseudomonas Putida S16, Vishakha Choudhary

Masters Theses

Our laboratory has previously shown that nicotine oxidoreductase (NicA2), a member of the flavin-containing amine oxidase family, uses a cytochrome c protein (CycN) as its electron acceptor rather than characteristically accepted molecular oxygen. Within the Pseudomonas putida S16 genome, the nicA2 gene forms an operon with cycN and pseudooxynicotine amine oxidase (pnao), another flavin containing amine oxidase enzyme. The first two steps in the nicotine catabolism pathway are catabolized by NicA2 and Pnao. Through redox chemistry, NicA2 converts nicotine to pseudooxynicotine and Pnao converts pseudooxynicotine to 3-succinoylsemialdehyde-pyridine. In this thesis, we characterize the kinetic and structural properties of …

Analysis Of The Zebrafish Olfactory System Using Immunohistochemistry And Enhanced Techniques Of Desorption Electrospray Ionization Mass Spectrometry (Desi-Ms), Tara Lynn Maser

Dissertations

Desorption electrospray ionization (DESI-MS) is an ambient ionization technique where the sample is analyzed directly from a surface with very minimal sample preparation under ambient conditions and follows ESI-like ionization mechanisms. DESI-MS has proven powerful in analyzing or imaging lipids and other small molecules directly from biological samples and even allows for subsequent histological staining and analyses. However, DESI-MS is less widely used for protein analysis due to a lack of sensitivity and the complex diversity of proteins in biological samples.

A major goal of this research has been to obtain new neurobiological knowledge by combining histology and mass spectrometry …

Lxr Acts As A Differentiator In The Regulation Of Fas And G6pdh Gene Expression Under Insulin Resistant Conditions, Jaafar Hachem

Dissertations

Diabetes is a chronic disease that effects 10 percent of the world’s population and causes more than 1.5 million deaths a year and billions of dollars in associated health care cost. It can lead to very serious complications such as renal failure, liver cirrhosis, heart attack, and vision loss. The most common type of diabetes is type 2 diabetes. Type 2 diabetes arises when blood glucose levels remain chronically high due to insulin resistance. The reason for this elevation is due to the failure of insulin to allow tissues to uptake glucose causing problems in subsequent metabolic pathways. Over the …

Mechanisms And Applications Of Improved Protein Analysis By Desorption Electrospray Ionization Mass Spectrometry (Desi-Ms), Roshan Javanshad

Dissertations

Electrospray ionization mass spectrometry (ESI-MS) is a soft ionization technique that allows detection of macromolecules, such as intact proteins, by the formation of multiply charged ions from solutions. Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient ionization technique that directly samples analyte from a surface during ESI-MS analysis. Although DESI-MS is highly accomplished at the analyses of metabolites, lipids, and other small molecules, it is far more limited when it comes to protein analysis. While most of the field in ambient ionization MS has moved towards primarily applications, our approach has been to explore the use of DESI-MS and …

Synthesis And Characterization Of Novel Organic Ligands With Their Complexes Of Platinum, Copper And Uranium, Mustafa Adnan Yasin Aldulaimi

Dissertations

Transition metal complexes of symmetrical and asymmetrical Schiff bases have played a significant role in the field of coordination, inorganic, and bioinorganic chemistry as models for biological, analytical, industrial, and pharmaceutical applications. Over recent years a great deal of interest has developed in new transition metal complexes of Schiff base ligand. The preparation of novel organic ligands is the most important step in the development of metal complexes that exhibit unique properties and novel reactivity. To highlight the presentation of this dissertation and to provide more detailed investigations, the dissertation was separated into six chapters according to the sequence of …

Development Of High Throughput Assays For Identification And Evaluation Of Small Molecule Inhibitors Of Microrna-31 Expression, Jackline A. Onyango

Masters Theses

Dysregulated expression of miRNAs has been linked to numerous cancers. On the other hand, miRNAs are potential drug targets due to the ability of their precursor molecules to fold into ligand binding structures. Small-molecule modulators of miRNAs offers opportunity for the development of new therapeutic agents and tools to further probe the mechanisms of miRNA functions. To facilitate the identification of miRNA modulators, the appropriate screening systems are needed, which would be adaptable for high-throughput applications, and allow for quantitative measurements. In this text, we present a molecular beacon-based assay to identify molecules that inhibit miRNA-31 processing by Dicer and …

Optimization Of Cation Exchange Chromatography Purification Of A Small Peptide: An Industrial And Practical Approach, Jamin Willoughby

Honors Theses

Ion exchange chromatography (IEX) is a common chemical separation technique that is used to separate ionizable target molecules from a liquid mixture mobile phase by adsorption of the molecule to a stationary phase. In chromatography, the stationary phase is a porous solid that the target molecule and mobile phase flows through, and is commonly referred to as the resin. Resins are commonly packed into hollow cylindrical columns. The mobile phase is the liquid solvent which flows through the column containing the stationary phase. The target molecule will have an affinity for the resin and adsorb to it, while impurities in …

Assessing The Robustness And Versatility Of Nanojars For Anion Binding, Mia Lily Ann Jawor

Masters Theses

Supramolecular chemistry is a field in chemistry that focuses on intermolecular bonds in a “host-guest complex” or supramolecular assemblies. Nanojars are supramolecular assemblies of the formula [anion⊂{Cu(μ-OH)(μ-pz)}_n] (n = 27‒33) which incarcerate kosmotropic anions, such as carbonate (CO₃^2‒), sulfate (SO₄^2‒) and hydrogen phosphate (HPO₄^2‒). Nanojars consist of three [Cu(μ-OH)(μ-pz)]_x (x = 6‒14, except 11) rings that are assembled similarly to Matryoshka dolls, where the top and bottom rings act as guests for the central ring. The larger central ring is bound to the two smaller rings by multiple …

Embedded Gold Nanoparticles For Metal Enhanced Photoluminescence, Hasna Alali

Dissertations

Noble metal nanoparticles (MNPs) have attracted great attention in electronics, solar cells and catalysis. Their unique optical properties and biocompatibility makes them useful in biological applications like imaging, drug delivery, therapy and diagnostic. At the surface of MNPs the collective oscillation of free electrons resonates with a particular wavelength of incident light, generating the Localized Surface Plasmons Resonance (LSPR). LSPR results in absorption and scattering of incident light. Scattering results in reflecting photons and absorption leads to enhanced photoluminescence and quenching of fluorophores, if the fluorophore is in the vicinity of MNPs.

Most of the studies in this regard have …

Biological Evaluation Of Fda-Approved Drugs As Mirna-31 Inhibitors Using Real Time Qrt-Pcr And A Luciferase Assay, Nicholas Sienkiewicz

Honors Theses

MicroRNAs (miRNAs) are a group of post-transcriptional, negative regulators of gene expression, consisting of ~19-24 nucleotides. Since their discovery in 1993, miRNAs have been found to be involved in a diverse amount of cellular and disease pathways, including certain types of cancer. Overexpression of certain oncogenic miRNAs, including miRNA-31, have been shown to play a role in cancer cell development. Recent findings have suggested that miRNAs play a significant role in every stage of colorectal cancer (CRC) initiation, progression, and development, as well as induce resistance to chemotherapy drugs like 5-FU. In particular, research has shown that increased levels of …

An Analysis Of Factors Influencing Chemistry Students’ Choice Of Major And Career, Jennifer Marie Ribble

Masters Theses

In recent years there has been a push to increase the number of STEM graduates in the United States in order for the nation to retain its position as a global leader in the sciences. If educators are to address recruitment and attrition issues in STEM fields it is important to understand student perspectives related to choosing both a major and a career in chemistry. The present study explores the decision-making process students utilize to choose chemistry as a major and as a career, their perception of what careers are available to someone with a degree in chemistry and what …

Smart Sensors With Dual Modes Of Signal Transduction For Monitoring Molecules Pertinent To Health And The Environment, Jared T. Wabeke

Dissertations

The dissertation focuses on the design and synthesis of smart materials for the detection of molecules pertinent to environmental protection and healthcare. The use of computational simulations is pivotal toward advancing molecular design for targeted applications. Research was conducted to investigate the use of simulations to develop novel sensors with dual modes of signal transduction. The molecular properties were determined using computational modelling, and then used to elucidate the binding mechanism of the corresponding sensor complexes. Several molecules were produced that respond to important organic analytes, such as glucose and fenthion, an organophosphorus pesticide. Glucose is an exceedingly important biological …

Ultrafast Relaxation Dynamics In Graphene Oxide-Dye And Perovskites Nanocomposites, Abubkr A. Arzaq Abuhagr

Dissertations

Novel materials such as graphene oxide (GO), reduced graphene oxide (RGO), and Perovskites nanocomposites nanosheets have shown interesting electrical and optical properties. These materials have shown prominence in research regarding optical sensing applications. The interaction of different fluorescent molecules like dye molecules with GO, and RGO have been studied recently to develop novel optical sensors, photo-catalysts, and light-harvesting agents. In this study, we have monitored the excited state interactions of dyes covalently attached to GO and RGO nanosheets. Three amine derivatives of anthracene, pyrene, and coumarin were covalently bound to different systems via amide bonds and diazotization. Characterization of different …

What A Career In Teaching Could Look Like For Me: My Journey Of Self-Reflection And Self-Discovery, Jashaun D. Bottoms

STEM Instructional Graduate Teaching Assistant Posters

I started the STEM Instructional Program the summer before my first teaching experience as a graduate student at WMU, and I saw it as a great training opportunity for the upcoming school year. As a General Chemistry II lab instructor, I would encounter students from various majors and backgrounds, with different learning styles and attitudes toward chemistry and math.

In order to effectively engage these students in a pre-lab lecture and to prepare them to complete the experiments, I needed to learn how to keep their attention, clearly explain and relay information in an interesting way, check their understanding, and …

Using Novel Spectroscopy Tool To Study Organized Self-Assemblies, Mohammad Rafe M Bin Hatshan

Dissertations

Organized self-assemblies are the cornerstones for countless biological processes and are integral parts of lipids, proteins, carbohydrates, nucleic acids, and cell membranes. Several man-made organized assemblies also play a vital role in interdisciplinary sciences that include micelles, reverse micelles, polymers, polyelectrolytes etc. Weak chemical interactions such as hydrogen bonding, p-p stacking, hydrophilic-hydrophobic and electrostatics often result in interesting organized self-assemblies. Understanding organized self-assemblies provides a huge opportunity to mimic naturally occurring biological macromolecules, design materials and develop strategies for specific applications. Several techniques are routinely used to understand the organized self-assemblies, and optical techniques play an important role in most …

Improving Protein Analysis By Desorption Electrospray Ionization (Desi-Ms), Elahe Honarvar

Dissertations

Electrospray ionization mass spectrometry (ESI-MS) is one of the most well-known and versatile techniques for analyzing a broad range of molecules and it has become one of the leading techniques to study biomolecules, such as proteins. ESI-MS can accurately determine the molecular weight of proteins and provide information about their peptide sequence, post-translational modifications as well as their interaction with other molecules.

During ESI-MS analysis, by spraying a sample of proteins, prepared in form of a solution, charged droplets are produced using an electric field. As the solvent molecules gradually evaporate from these droplets, freely hovering bare protein ions remain. …

Best Organic Chemistry Practices In The Midwest, Claire Van Der Bosch

Honors Theses

Organic Chemistry is a gateway course to a handful of courses in higher chemical education. Unfortunately, it is notorious for its high-fail, high-withdrawal, and high-drop rate and has a reputation for being time-consuming, with difficult concepts that are oftentimes new to students. It is often taught in a two-semester sequence, with the courses named Organic I and Organic II, and both courses have a lab component. At Western Michigan University, the fail rate for these two courses are 53.5% and 36.9%, respectively. Most higher education institutions are certified with the American Chemical Society (ACS) and given a skeleton of a …

The Effects Of Climate Change And Forest Disturbances On Terpene Production Of White Pine (Pinus Strobus) And Red Pine (Pinus Resinosa) In Northern Michigan, Troy Dehagen

Honors Theses

The secondary impacts that climate change may have on the interactions between forests and the atmosphere through forest disturbances is of increasing interest as the climate continues to change. Anthropogenic climate change, which is a direct result of coal and fossil fuel emissions, is likely to impact forest ecology through increased temperatures and extreme precipitation events, thus increasing the frequency and severity of climate-induced forest disturbances. Forest disturbances may have a secondary impact on the production and emission of biogenic volatile organic compounds (BVOC) within forests. In the presence of oxides of nitrogen (NO_x) produced from fossil fuel …

Stylolites Cathodoluminescence Under Cross Polarized Light, Katherine Dvorak

The Hilltop Review

Devil's Gate limestone under cathodoluminescence. Bright yellow and orange areas are calcite and the bright blue indicates trace minerals along the solution seam. Trace minerals left in the seam are heavy metals and other silicate minerals.

Computational Study Of The Nature Of Tetrel Bond, Yama Aman

Masters Theses

For more than a hundred years, the type of a chemical bond has been distinguished according to the relative electronegativities of chemical units at the ends of a bond. Whereas, the bond of unequally electronegative on both ends is called a polar covalent or even ionic bond or a non-polar covalent bond in case of both equal. Regardless the type of bonded elements, the whole idea for the bond was believed to be electron driven only. In the 20th century the development of the idea, that not just the electronic relation but specific elements are also able to characterize the …

Special Elements Of Excellence: Summer 2018, Department Of Chemistry

Chemistry News

• From the Chair's Desk
• 1st Collaborative Research Gathering
• Emerging Scholar Award
• Associate Prof Andre Venter reports on his sabbatical at the University of Pierre and Marie Curie in Paris, France
• 2017 Spring Award Recipients
• James Kiddle, Recipient of the Local Section Outreach Volunteer of the Year Award

Nanojars: Versatile Platform For Pyrazole Synthesis And Chemistry, And Selective Extraction Of Anions, Basil Mohammed Ahmed

Dissertations

This work is centered around nanojars, a family of nano-sized anion-incarcerating agents. It addresses various aspects of nanojar chemistry, such as synthesis strategies (including green methodologies) for pyrazole ligands needed for the preparation of nanojars, characterization and study of the mechanism of assembly of nanojars, and selective extraction of anions from water using nanojars.

Nanojars are neutral, supramolecular coordination complexes of the general formula [anion⊂ {Cu(μ-OH)(μ-pz)}_n], formed by the reaction of Cu²⁺, HO^‒ and pyrazolate ions (C₃H₂N₂‒, pz^‒) in the presence of oxoanions with large hydration energy …

Synthesis And Characterization Of Fluorescent Nanojars, Vageesha Warnajith Liyana Gunawardana

Masters Theses

Self-assembly is a powerful tool utilized by synthetic chemists to create large, intricate structures for a variety of applications such as drug delivery, adaptable materials and electronics. In contrast to stepwise synthesis, this process allows the formation of intricate molecular architectures starting from synthetically rudimentary building blocks. Nanojars are class of self-assembling, cyclic copper(II) pyrazolate coordination compounds that encapsulate oxoanions with negative two or three charge. The strong anion binding characteristics of nanojars have potential towards developing novel tools for detecting biologically and environmentally relevant anions such as carbonate, sulfate and phosphate.

This work addresses the design and synthesis of …

Elucidation Of The Toxicity Of Anthropogenic Nanoparticles In Environments Containing Heavy Metals, Chartanay Bonner

Dissertations

The design, synthesis, and applications of anthropogenic nanoparticles continue to rise as technologies utilizing the unique chemical and physical properties of materials with nanoscale dimensions emerge. Engineered silver nanoparticles (Ag NPs) possess antimicrobial properties toward several strains of antibiotic-resistant bacteria. The manufacturing and use of Ag NPs have led to their increased presence in the environment as pollutants. The interaction of Ag NPs with other pollutants influence their surface properties, aggregation state, stability and consequently, their toxic response toward microbial pathogens. In the present study, we examined the interaction of heavy metal ions (Cd²⁺, Hg²⁺, and …

Organocatalyzed Synthesis Of Epoxides From Chalcones Utilizing Amino Acids, Sabrina N. Kegeler

Masters Theses

The epoxide functional group is important throughout the chemical and pharmaceutical industries, as well as in nature. In the chemical industry, epoxides are present in resins and fragrances. In the pharmaceutical industry, epoxide-containing compounds are used as intermediates in the manufacturing of drugs. In nature, many natural products contain epoxide groups and are used for medicinal purposes, and for models to create synthetic molecules.

One approach to epoxide synthesis involves the use of an alkene precursor, a base, and an oxidizing agent. This is where my investigations began. The first step was to optimize the epoxidation reaction, examining substrate scope, …

Apan Production Through Photooxidation Of Isoprene In The Presence Of NoX, Timothy Glidewell

Masters Theses

Peroxyacyl nitrates (PANs, RC(O)OONO₂) are of great importance in atmospheric chemistry. They act as transporters of reactive nitrogen, and radical delivery agents. There are no known natural emission sources of PANs; they are produced through reaction of volatile organic compounds (VOCs) and nitrogen oxides (NO_x) in the atmosphere.

VOCs can enter the atmosphere through biogenic and anthropogenic pathways. It has been estimated that isoprene makes up 70% of the biogenic VOCs (BVOCs) emitted annually. Isoprene has been shown to impact ozone levels in both rural and urban areas by sequestering and transporting NO_x in the form …

Structures Of Metallacarboranes And Coordination Complexes: Coordination Sensors Based On Rhodamine B And Quinolines, Agozie Nnaemeka Oyeamalu

Dissertations

Rhodamine-based ligands that can complex different metals has been designed and synthesized. Using Rhodamine B as starting material, we synthesized and characterized quinoline precursors as well as successfully carried out the oxidation of methyl and aldehyde groups on the quinoline ring, and on the hydroxyl moiety of the quinoline spirolactone ring. These turn-on/off rhodamine fluorescence probes sense Cr³⁺ and Ni²⁺ with high selectivity and sensitivity. These probes can be applied to detect other metal ions that are present in chemical, biological and environmental settings. They were designed to fluoresce when bonded to ions such as cyanide in detecting …