Physical Sciences and Mathematics Commons^™

The Interaction Of Naphthoquinones With The Calcium Release Channel Of Skeletal Muscle Sarcoplasmic Reticulum, Ruohong Xia

Dissertations and Theses

The sarcoplasmic reticulum (SR) is an intracellular membrane system which regulates cytoplasmic calcium concentration in muscle and controls the contractile state of muscle. In this thesis, the interaction between naphthoquinone and the Ca²⁺ release mechanism of SR is described. 1,4-naphthoquinone (1,4NQ) is shown to stimulate Ca²⁺ release and to modify high-affinity ryanodine binding to skeletal muscle sarcoplasmic reticulum. The interaction between 1,4NQ and the SR involves the oxidation ofcritical sulfhydryl groups associated with the Ca²⁺ release mechanism. The modulation of ryanodine binding by 1,4NQ is biphasic. At low concentrations of 1,4NQ (<10 >μM) ryanodine binding is stimulated, …