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Full-Text Articles in Physical Sciences and Mathematics
Toxicokinetics And Metabolism Of Aniline And 4-Chloroaniline In Medaka (Oryzias Latipes), Steven P. Bradbury, Julia M. Dady, Patrick N. Fitzsimmons, Michelle M. Voit, Dean E, Hammermeister, Russell J. Erickson
Toxicokinetics And Metabolism Of Aniline And 4-Chloroaniline In Medaka (Oryzias Latipes), Steven P. Bradbury, Julia M. Dady, Patrick N. Fitzsimmons, Michelle M. Voit, Dean E, Hammermeister, Russell J. Erickson
Steven P. Bradbury
The in vivo toxicokinetics and in vitro hepatic microsomal metabolism of (14C)aniline and (14C)4-chloroaniline in medaka (Oryzias latipes) were investigated to provide a basis upon which to interpret the toxicological responses of small aquarium fish to aniline derivatives. During static aqueous exposures of up to 320 min, parent equivalents failed to reach steady state and results from depuration studies clearly demonstrated biphasic elimination. Due to low elimination rates, 40 to 20% of absorbed aniline and 4-chloroaniline doses, respectively, remained within the fish through 330 min postexposure. Based on an analysis of excreted metabolites, N-acetylation was the dominant route of in …
Influence Of Cytochrome P450 Mixed-Function Oxidase Induction On The Acute Toxicity To Rainbow Trout (Salmo Gairdneri) Of Primary Aromatic Amines, Steven P. Bradbury, Joop L. M. Hermens, Steven J. Broderius
Influence Of Cytochrome P450 Mixed-Function Oxidase Induction On The Acute Toxicity To Rainbow Trout (Salmo Gairdneri) Of Primary Aromatic Amines, Steven P. Bradbury, Joop L. M. Hermens, Steven J. Broderius
Steven P. Bradbury
The influence of enzyme induction on the acute toxicity of aniline and 4-chloroaniline to rainbow trout (Salmo gairdneri) was investigated. For these two xenobiotics, bioactivation reactions are known to occur in mammals. Induction of cytochrome P450 mixed-function oxidase was obtained by intraperitoneal (ip) injection of trout with a mixture of polychlorinated biphenyls (Aroclor 1254). Five days after ip injection with three different doses of Aroclor 1254 (50. 100. and 200 mg/kg). benzo[a)pyrene hydroxylase activity in trout liver microsomes increased five- to sixfold. Cytochrome P450 concentrations in the microsomes were slightly. but significantly. enhanced in two of the three dose levels. …