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Physical Sciences and Mathematics Commons™

Open Access. Powered by Scholars. Published by Universities.®

Life Sciences

2017

University of Missouri, St. Louis

Articles 1 - 10 of 10

Full-Text Articles in Physical Sciences and Mathematics

2-D Electrophoresis Modeling Of Multienzyme Cutting Of Polypeptides, Howard Mayes Oct 2017

2-D Electrophoresis Modeling Of Multienzyme Cutting Of Polypeptides, Howard Mayes

Theses

2-Dimensional Electrophoresis is one of the tools in the identification of proteins by molecular weight and pH. The display of molecular weight allows the researcher to quickly identify whether a specific protein or peptide string is in the sample. The pH measurement allows even better resolution between different species in the sample. The MultiEnzyme ElectroPhoresis (MEEP) program tries to model that by providing a graph that displays separated protein strings by both molecular weight and pH. The ability to cleave the protein with 43 different enzyme variations allows the researcher to analyze appropriate enzymes to isolate a protein subsequence before …

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First-Principles And Model Simulation Of All-Optical Spin Reversal, Thomas George, G.P. Zhang, Z. Babyak, Y. Xue, Y. Bai Oct 2017

First-Principles And Model Simulation Of All-Optical Spin Reversal, Thomas George, G.P. Zhang, Z. Babyak, Y. Xue, Y. Bai

Chemistry & Biochemistry Faculty Works

All-optical spin switching is a potential trailblazer for information storage and communication at an unprecedented fast rate free of magnetic fields. However, the current wisdom is largely based on semiempirical models of effective magnetic fields and heat pulses, so it is difficult to provide high-speed design protocols for actual devices. Here, we carry out a massively parallel first-principles and model calculation for 13 spin systems and magnetic layers, free of any effective field, to establish a simpler and alternative paradigm of laser-induced ultrafast spin reversal and to point out a path to a full-integrated photospintronic device. It is the interplay …

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Biophysical And Computational Studies Of The Vcci:Vmip-Ii Complex, Anna Nguyen, Nai-Wei Kuo, Laura Showalter, Ricardo Ramos, Cynthia Dupureur, Michael Colvin, Patricia Liwang Aug 2017

Biophysical And Computational Studies Of The Vcci:Vmip-Ii Complex, Anna Nguyen, Nai-Wei Kuo, Laura Showalter, Ricardo Ramos, Cynthia Dupureur, Michael Colvin, Patricia Liwang

Chemistry & Biochemistry Faculty Works

Certain viruses have the ability to subvert the mammalian immune response, including interference in the chemokine system. Poxviruses produce the chemokine binding protein vCCI (viral CC chemokine inhibitor; also called 35K), which tightly binds to CC chemokines. To facilitate the study of vCCI, we first provide a protocol to produce folded vCCI from Escherichia coli (E. coli.) It is shown here that vCCI binds with unusually high affinity to viral Macrophage Inflammatory Protein-II (vMIP-II), a chemokine analog produced by the virus, human herpesvirus 8 (HHV-8). Fluorescence anisotropy was used to investigate the vCCI:vMIP-II complex and shows that vCCI binds to …

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Rational Drug Design Directed At Blocking The Initial Signaling Events In Lipopolysaccharide-Induced Sepsis., Christopher A. Tipton Jul 2017

Rational Drug Design Directed At Blocking The Initial Signaling Events In Lipopolysaccharide-Induced Sepsis., Christopher A. Tipton

Theses

Systemic Inflammatory Response Syndrome (SIRS) is classified as an immune system response to an infectious state. If left untreated, SIRS leads to sepsis, septic shock, end-organ dysfunction, and death. As a patient progresses through these stages, associations of acute respiratory distress, disseminated intravascular coagulation, and acute renal failure persist, resulting in millions of deaths annually. Lipopolysaccharide (LPS), a bacterial endotoxin, is released into the blood stream, triggering SIRS. LPS is found in the outer cell-wall of Gram-negative bacteria and is responsible for initiation of a devastating cytokine storm. One of the regions of LPS, lipid A, is a polyacylated glucosamine …

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Beyond 3 Au From The Sun: The Hypervolatiles Ch4, C2h6, And Co In The Distant Comet C/2006 W3 (Christensen), Boncho Bonev, Geronimo Villanueva, Michael Disanti, Hermann Boehnhardt, Manuela Lippi, Erika Gibb, Lucas Paganini, Michael Mumma May 2017

Beyond 3 Au From The Sun: The Hypervolatiles Ch4, C2h6, And Co In The Distant Comet C/2006 W3 (Christensen), Boncho Bonev, Geronimo Villanueva, Michael Disanti, Hermann Boehnhardt, Manuela Lippi, Erika Gibb, Lucas Paganini, Michael Mumma

Physics Faculty Works

Comet C/2006 W3 (Christensen) remained outside a heliocentric distance (R h) of 3.1 au throughout its apparition, but it presented an exceptional opportunity to directly sense a suite of molecules released from its nucleus. The Cryogenic Infrared Echelle Spectrograph at ESO-VLT detected infrared emissions from the three "hypervolatiles" (CO, CH4, and C2H6) that have the lowest sublimation temperatures among species that are commonly studied in comets by remote sensing. Even at R h = 3.25 au, the production rate of each molecule exceeded those measured for the same species in a number of other comets, although these comets were observed …

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Network Exploration Of Correlated Multivariate Protein Data For Alzheimer's Disease Association, Matthew J. Lane Apr 2017

Network Exploration Of Correlated Multivariate Protein Data For Alzheimer's Disease Association, Matthew J. Lane

Theses

Alzheimer Disease (AD) is difficult to diagnose by using genetic testing or other traditional methods. Unlike diseases with simple genetic risk components, there exists no single marker determining as to whether someone will develop AD. Furthermore, AD is highly heterogeneous and different subgroups of individuals develop the disease due to differing factors. Traditional diagnostic methods using perceivable cognitive deficiencies are often too little too late due to the brain having suffered damage from decades of disease progression. In order to observe AD at early stages prior to the observation of cognitive deficiencies, biomarkers with greater accuracy are required. By using …

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The Composition Of Comet C/2012 K1 (Panstarrs) And The Distribution Of Primary Volatile Abundances Among Comets, Nathan Roth, Erika Gibb, Boncho Bonev, Michael Disanti, Michael Mumma, Geronimo Villanueva, Lucas Paganini Mar 2017

The Composition Of Comet C/2012 K1 (Panstarrs) And The Distribution Of Primary Volatile Abundances Among Comets, Nathan Roth, Erika Gibb, Boncho Bonev, Michael Disanti, Michael Mumma, Geronimo Villanueva, Lucas Paganini

Physics Faculty Works

On 2014 May 22 and 24 we characterized the volatile composition of the dynamically new Oort cloud comet C/2012 K1 (PanSTARRS) using the long-slit, high resolution (λ/Δλ ≈ 25,000) near-infrared echelle spectrograph (NIRSPEC) at the 10 m Keck II telescope on Maunakea, Hawaii. We detected fluorescent emission from six primary volatiles (H2O, HCN, CH4, C2H6, CH3OH, and CO). Upper limits were derived for C2H2, NH3, and H2CO. We report rotational temperatures, production rates, and mixing ratios (relative to water). Compared with median abundance ratios for primary volatiles in other sampled Oort cloud comets, trace gas abundance ratios in C/2012 K1 …

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Cyclipostins And Cyclophostin Analogs As Promising Compounds In The Fight Against Tuberculosis, Phuong Nguyen, Vincent Delorme, Anaïs Bénarouche, Benjamin Martin, Rishi Paudel, Giri Gnawali, Abdeldjalil Madani, Rémy Puppo, Valérie Landry, Laurent Kremer, Priscille Brodin, Christopher Spilling, Jean-François Cavalier, Stéphane Canaan Jan 2017

Cyclipostins And Cyclophostin Analogs As Promising Compounds In The Fight Against Tuberculosis, Phuong Nguyen, Vincent Delorme, Anaïs Bénarouche, Benjamin Martin, Rishi Paudel, Giri Gnawali, Abdeldjalil Madani, Rémy Puppo, Valérie Landry, Laurent Kremer, Priscille Brodin, Christopher Spilling, Jean-François Cavalier, Stéphane Canaan

Chemistry & Biochemistry Faculty Works

A new class of Cyclophostin and Cyclipostins (CyC) analogs have been investigated against Mycobacterium tuberculosis H37Rv (M. tb) grown either in broth medium or inside macrophages. Our compounds displayed a diversity of action by acting either on extracellular M. tb bacterial growth only, or both intracellularly on infected macrophages as well as extracellularly on bacterial growth with very low toxicity towards host macrophages. Among the eight potential CyCs identified, CyC 17 exhibited the best extracellular antitubercular activity (MIC50 = 500 nM). This compound was selected and further used in a competitive labelling/enrichment assay against the activity-based probe Desthiobiotin-FP in order …

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Evolution Of Floral Zygomorphy In Androecium And Corolla In Solanaceae, Jingbo Zhang, Peter Stevens, Wenheng Zhang Jan 2017

Evolution Of Floral Zygomorphy In Androecium And Corolla In Solanaceae, Jingbo Zhang, Peter Stevens, Wenheng Zhang

Biology Department Faculty Works

In Solanaceae, a group dominated by actinomorphic‐flowered species, floral zygomorphy is frequently observed among the early‐branching clades. Morphological studies indicated that a zygomorphic androecium is much more common than a zygomorphic corolla in the family. Ontogenic studies suggested the evolution of floral zygomorphy in these two whorls is independent. Here, we have examined the evolution of floral symmetry in the androecium and corolla in Solanaceae. The character states of floral symmetry were assembled for androecium and corolla separately, and ancestral state reconstructions were carried out at both the genus and species levels for Solanaceae and its outgroups. Correlation tests were …

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Phylogenetic Patterns Of Rarity In A Regional Species Pool Of Tropical Woody Plants, M. Loza, Iván Jiménez, Peter Jørgensen, Gabriel Arellano, Manuel Macía, Vania Torrez, Robert Ricklefs Jan 2017

Phylogenetic Patterns Of Rarity In A Regional Species Pool Of Tropical Woody Plants, M. Loza, Iván Jiménez, Peter Jørgensen, Gabriel Arellano, Manuel Macía, Vania Torrez, Robert Ricklefs

Biology Department Faculty Works

AimRarity, which is believed to influence extinction risk, can be defined in terms of local abundance, geographical range size and habitat breadth. Phylogenetic patterns in these attributes provide insight into the extent to which rarity and extinction risk are conserved during evolution and the potential for species‐level heritability. We evaluated phylogenetic signal (i.e., related species resembling each other more than species drawn at random) and evolutionary conservatism (similarity among related species exceeding that expected from a Brownian model of evolution) in three axes of rarity (local abundance, geographical range size and habitat breadth) among species in a regional pool of …

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