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Articles 1 - 8 of 8
Full-Text Articles in Physical Sciences and Mathematics
Identification Of Phospholipids In Human Meibum By Nano-Electrospray Ionisation Tandem Mass Spectrometry, Jennifer T. Saville, Zhenjun Zhao, Mark Dp Willcox, Manjula A. Ariyavidana, Stephen J. Blanksby, Todd W. Mitchell
Identification Of Phospholipids In Human Meibum By Nano-Electrospray Ionisation Tandem Mass Spectrometry, Jennifer T. Saville, Zhenjun Zhao, Mark Dp Willcox, Manjula A. Ariyavidana, Stephen J. Blanksby, Todd W. Mitchell
Stephen Blanksby
Meibum is believed to be the major source of tear !lm lipids, which are vital in the prevention of excessevaporation of the aqueous phase. The complete lipid composition of meibum has yet to be established.While earlier studies reported the presence of phospholipids in human meibum, recent mass spectrometricstudies have not detected them. In this study we use electrospray ionisation tandem massspectrometry to investigate the presence of phospholipids in meibum and provide comparison to thephospholipid pro!le of tears.Lipids were extracted from human meibum and tear samples using standard biphasic methods andanalysed by nano-electrospray ionisation tandem mass spectrometry using targeted ion scans. …
Defining The Structural Basis Of Human Plasminogen Binding By Streptococcal Surface Enolase, Amanda J. Cork, Slobodan Jergic, Sven Hammerschmidt, Bostjan Kobe, Vijay Pancholi, Justin L.P. Benesch, Carol V, Robinson, Nicholas E. Dixon, J Andrew Aquilina, Mark J. Walker
Defining The Structural Basis Of Human Plasminogen Binding By Streptococcal Surface Enolase, Amanda J. Cork, Slobodan Jergic, Sven Hammerschmidt, Bostjan Kobe, Vijay Pancholi, Justin L.P. Benesch, Carol V, Robinson, Nicholas E. Dixon, J Andrew Aquilina, Mark J. Walker
J. A. Aquilina
The flesh-eating bacterium group A Streptococcus (GAS) binds and activates human plasminogen, promoting invasive disease. Streptococcal surface enolase (SEN), a glycolytic pathway enzyme, is an identified plasminogen receptor of GAS. Here we used mass spectrometry (MS) to confirm that GAS SEN is octameric, thereby validating in silico modeling based on the crystal structure of S. pneumoniae -enolase. Site-directed mutagenesis of surface-located lysine residues (SENK252+255A, SENK304A, SENK334A, SENK344E, SENK435L and SEN434-435) was used to examine their roles in maintaining structural integrity, enzymatic function and plasminogen binding. Structural integrity of the GAS SEN octamer was retained for all mutants except SENK344E, as …
Greening Rural Festivals: Ecology, Sustainability And Human-Nature Relations, Christopher R. Gibson, C Wong
Greening Rural Festivals: Ecology, Sustainability And Human-Nature Relations, Christopher R. Gibson, C Wong
Chris Gibson
No abstract provided.
Activity And Molecular Dynamics Relationship Within The Family Of Human Cholinesterase, Judith Peters, Marie Trovaslet, Marcus Trapp, Florian Nachon, Flynn Hill, Etienne Royer, Frank Gabel, Lambert Van Eijck, Patrick Masson, Moeava Tehei
Activity And Molecular Dynamics Relationship Within The Family Of Human Cholinesterase, Judith Peters, Marie Trovaslet, Marcus Trapp, Florian Nachon, Flynn Hill, Etienne Royer, Frank Gabel, Lambert Van Eijck, Patrick Masson, Moeava Tehei
Faculty of Science - Papers (Archive)
The temperature dependence of the dynamics of recombinant human acetylcholinesterase (hAChE) and plasma human butyrylcholinesterase (hBChE) is examined using elastic incoherent neutron scattering. These two enzymes belong to the same family and present 50% amino acid sequence identity. However, significantly higher flexibility and catalytic activity of hAChE when compared to the ones of hBChE are measured. At the same time, the average height of the potential barrier to the motions is increased in the hBChE, e.g. more thermal energy is needed to cross it in the latter case, which might be the origin of the increase in activation energy and …
Molecular Characterization Of A 21.4 Kilobase Antibiotic Resistance Plasmid From An Hemolytic Escherichia Coli O108:H-Human Clinical Isolate, Fay E. Dawes, Dieter M. Bulach, Alexander Kuzevski, Karl A. Bettelheim, Carola Venturini, Steven P. Djordjevic, Mark J. Walker
Molecular Characterization Of A 21.4 Kilobase Antibiotic Resistance Plasmid From An Hemolytic Escherichia Coli O108:H-Human Clinical Isolate, Fay E. Dawes, Dieter M. Bulach, Alexander Kuzevski, Karl A. Bettelheim, Carola Venturini, Steven P. Djordjevic, Mark J. Walker
Faculty of Science - Papers (Archive)
This study characterizes the 21.4 kilobase plasmid pECTm80 isolated from Escherichia coli strain 80, an α hemolytic human clinical diarrhoeal isolate (serotype O108:H-). DNA sequence analysis of pECTm80 revealed it belonged to incompatibility group X1, and contained plasmid partition and toxin-antitoxin systems, an R6K-like triple origin (ori) replication system, genes required for replication regulation, insertion sequences IS1R, ISEc37 and a truncated transposase gene (Tn3-like ΔtnpA) of the Tn3 family, and carried a class 2 integron. The class 2 integron of pECTm80 contains an intact cassette array dfrA1-sat2, encoding resistance to trimethoprim and streptothricin, …
Beeswax As Dental Filling On A Neolithic Human Tooth, Federico Bernardini, Claudio Tuniz, Alfredo Coppa, Lucia Mancini, Diego Dreossi, Diane Eichert, Gianluca Turco, Matteo Biasotto, Filippo Terrasi, Nicola De Cesare, Quan Hua, Vladimir Levchenko
Beeswax As Dental Filling On A Neolithic Human Tooth, Federico Bernardini, Claudio Tuniz, Alfredo Coppa, Lucia Mancini, Diego Dreossi, Diane Eichert, Gianluca Turco, Matteo Biasotto, Filippo Terrasi, Nicola De Cesare, Quan Hua, Vladimir Levchenko
Faculty of Science - Papers (Archive)
Evidence of prehistoric dentistry has been limited to a few cases, the most ancient dating back to the Neolithic. Here we report a 6500-year-old human mandible from Slovenia whose left canine crown bears the traces of a filling with beeswax. The use of different analytical techniques, including synchrotron radiation computed micro-tomography (micro-CT), Accelerator Mass Spectrometry (AMS) radiocarbon dating, Infrared (IR) Spectroscopy and Scanning Electron Microscopy (SEM), has shown that the exposed area of dentine resulting from occlusal wear and the upper part of a vertical crack affecting enamel and dentin tissues were filled with beeswax shortly before or after the …
P2x7 Receptor Activation Mediates Organic Cation Uptake Into Human Myeloid Leukaemic Kg-1 Cells, Safina Gadeock, Aleta Pupovac, Vanessa Sluyter, Mari Spildrejorde, Ronald Sluyter
P2x7 Receptor Activation Mediates Organic Cation Uptake Into Human Myeloid Leukaemic Kg-1 Cells, Safina Gadeock, Aleta Pupovac, Vanessa Sluyter, Mari Spildrejorde, Ronald Sluyter
Faculty of Science - Papers (Archive)
The P2X7 purinergic receptor is an ATP-gated cation channel with an emerging role in neoplasia. In this study we demonstrate that the human KG-1 cell line, a model of acute myelogenous leukaemia, expresses functional P2X7. RT-PCR and immunochemical techniques demonstrated the presence of P2X7 mRNA and protein respectively in KG-l cells, as well as in positive control multiple myeloma RPMI 8226 cells. Flow cytometric measurements demonstrated that ATP induced ethidium(+) uptake into KG-l cells suspended in sucrose medium (EC(50) of ∼3 μM), but not into cells in NaCl medium. In contrast, ATP induced ethidium(+) uptake into RPMI 8226 cells suspended …
Structural Insights Into The Dehydroascorbate Reductase Activity Of Human Omega-Class Glutathione Transferases, Huina Zhou, Joseph Brock, Dan Liu, Philip G. Board, Aaron J. Oakley
Structural Insights Into The Dehydroascorbate Reductase Activity Of Human Omega-Class Glutathione Transferases, Huina Zhou, Joseph Brock, Dan Liu, Philip G. Board, Aaron J. Oakley
Faculty of Science - Papers (Archive)
The reduction of dehydroascorbate (DHA) to ascorbic acid (AA) is a vital cellular function. The omega-class glutathione transferases (GSTs) catalyze several reductive reactions in cellular biochemistry, including DHA reduction. In humans, two isozymes (GSTO1-1 and GSTO2-2) with significant DHA reductase (DHAR) activity are found, sharing 64% sequence identity. While the activity of GSTO2-2 is higher, it is significantly more unstable in vitro. We report the first crystal structures of human GSTO2-2, stabilized through site-directed mutagenesis and determined at 1.9Å resolution in the presence and absence of glutathione (GSH). The structure of a human GSTO1-1 has been determined at 1.7Å resolution …