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Full-Text Articles in Veterinary Medicine
Role Of Herpes Simplex Type I Glycoproteins In Entry And Cell-Cell Fusion, Sona Chowdhury
Role Of Herpes Simplex Type I Glycoproteins In Entry And Cell-Cell Fusion, Sona Chowdhury
LSU Doctoral Dissertations
Herpes simplex virus type I (HSV-1) is a neurotropic virus that infects primarily mucocutaneous epithelial cells and nervous tissue. Membrane fusion is an important aspect of the HSV-1 lifecycle, that occurs during viral entry (virus-cell fusion), viral spread (cell-to-cell fusion), as well as, during virion morphogenesis (assembly and egress). These membrane fusion steps involve complex interactions between multiple viral glycoproteins and cellular receptors. HSV-1 glycoprotein B (gB) is necessary but not sufficient for membrane fusion events. Despite the fact, that the majority of known hypermorphic mutations which cause extensive virus-induced cell fusion occur within glycoprotein K (gK); yet the role …
Zebrafish Kidney Phagocytes Utilize Macropinocytosis And Ca+-Dependent Endocytic Mechanisms., Claudia Hohn, Sang-Ryul Lee, Lesya M. Pinchuk, Lora Petrie-Hanson
Zebrafish Kidney Phagocytes Utilize Macropinocytosis And Ca+-Dependent Endocytic Mechanisms., Claudia Hohn, Sang-Ryul Lee, Lesya M. Pinchuk, Lora Petrie-Hanson
College of Veterinary Medicine Publications and Scholarship
BACKGROUND: The innate immune response constitutes the first line of defense against invading pathogens and consists of a variety of immune defense mechanisms including active endocytosis by macrophages and granulocytes. Endocytosis can be used as a reliable measure of selective and non-selective mechanisms of antigen uptake in the early phase of an immune response. Numerous assays have been developed to measure this response in a variety of mammalian and fish species. The small size of the zebrafish has prevented the large-scale collection of monocytes/macrophages and granulocytes for these endocytic assays. METHODOLOGY/PRINCIPAL FINDINGS: Pooled zebrafish kidney hematopoietic tissues were used as …
Porcine Reproductive And Respiratory Syndrome Virus Enters Cells Through A Low Ph-Dependent Endocytic Pathway, Luiz C. Kreutz, Mark R. Ackermann
Porcine Reproductive And Respiratory Syndrome Virus Enters Cells Through A Low Ph-Dependent Endocytic Pathway, Luiz C. Kreutz, Mark R. Ackermann
Mark R. Ackermann
The entry pathway of porcine reproductive and respiratory syndrome virus (PRRSV) into MARC-145 cells was investigated using a variety of drugs that interfere with the pH of intracellular vesicles by different mechanisms. Virus entry was assessed by measuring viral RNA replication or production of infectious virus. Chloroquine, ammonium chloride and bafilomycin A1 inhibited RNA replication or production of infectious virus in a dose-dependent manner. These drugs inhibited virus replication when added to the cells prior to, at infection or soon after infection. Moreover, the effect of chloroquine on PRRSV replication was reversible under acidic conditions of the media. Taken together, …