Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Organisms
Development Of A Novel Ex Vivo Equine Corneal Model, Todd L. Marlo, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan
Development Of A Novel Ex Vivo Equine Corneal Model, Todd L. Marlo, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan
Pharmacy Faculty Articles and Research
Objective
To develop an ex vivo equine corneal organ culture model. Specifically, to assess the equine cornea's extracellular matrix and cellularity after 7 days using two different culture techniques: either (i) immersion system or (ii) air/liquid interface system, to determine the best ex vivo equine corneal model.
Animals Studied
Fourteen healthy equine corneas of various breeds.
Procedures
Equine corneas with 2 mm of perilimbal sclera were freshly harvested from 7 horses undergoing humane euthanasia. One corneal–scleral ring (CSR) from each horse was randomly placed in the (i) immersion condition organ culture system (IC), with the contralateral CSR being placed in …