Organisms Commons^™

Evaluation Of Animal Model Research, Kenneth J. Shapiro

Kenneth J. Shapiro, PhD

It is argued that a concept of evaluation of animal models that is broader and more useful than validation is available. Productive generativity refers to the degree to which a model furthers understanding and leads to more-effective treatment interventions. Results of the application of this novel evaluative frame to several animal models of eating disorders show that this animal-based research has not been productive. The question of the relation between clinic and animal laboratory is discussed.

Olfactory Enrichment In California Sea Lions (Zalophus Californianus), Mystera M. Samuelson

Dissertations

In the wild, California sea lions (Zalophus californianus) are exposed to a wide array of sensory information at all times. However, it is impossible for captive environments to provide this level of complexity. Therefore unique procedures and practices are necessary for the maintenance of physiological and psychological health in captive animals (Wells, 2009). This project aims to explore the behavioral effect of scent added to the environment, with the goal of improving the welfare of captive sea lions by introducing two scent types: 1.) Natural scents, found in their native environment, and 2.) Non-natural scents, not found in …

Anti-Tb And Antibacterial Activities Of Natural Products Extracts, Douglas Armstrong, Nathan Krause, Drew Frey

Faculty Scholarship – Chemistry

Samples of numerous plant species were received from the southwestern part of the USA from Richard Spjut, and plant samples were collected here in Illinois. All were extracted with typical solvents, giving crude residues, some of which were subjected to counter-current or flash chromatographic methods. Some of the crude extracts and chromatographic fractions had anti-tuberculosis and/or antibacterial activity.

In a general way, bioactive natural products are dealt with very well by Liang & Fang, 2006. More specifically, the southwestern part of the United States has a large variety of indigenous plants, many of which have not been investigated for their …

Rapid Label-Free Detection Of E. Coli Using Antimicrobial Peptide Assisted Impedance Spectroscopy, Keren Jieng, Hashem Etayash, Sarfuddin Azmi, Selvaraj Naicker, Mahtab Hassanpourfard, Parmiss Mojir Shaibani, Garima Thakur, Kamaljit Kaur, Thomas Thundat

Pharmacy Faculty Articles and Research

There is an increasing demand for rapid detection of waterborne pathogens to monitor drinking water safety. We demonstrate a compact, label-free sensor array for rapid detection of Escherichia coli (E. coli) in contaminated water samples using antimicrobial peptide assisted impedimetric sensor platform. Interdigitated electrode arrays immobilized with the antimicrobial peptide Colicin V (ColV) were used to screen the affinity towards different bacterial strains by monitoring impedance variations in real-time. This ColV assisted impedance biosensor exhibited high selectivity towards Gram-negative strains particularly towards E. coli strains. This selective detection of E. coli from other strains was observed at 10 …

Expectations For Methodology And Translation Of Animal Research: A Survey Of Health Care Workers, Ari Joffe, Meredith Bara, Natalie Anton, Nathan Nobis

Nathan M. Nobis, PhD

Background: Health care workers (HCW) often perform, promote, and advocate use of public funds for animal research (AR); therefore, an awareness of the empirical costs and benefits of animal research is an important issue for HCW. We aim to determine what health-care-workers consider should be acceptable standards of AR methodology and translation rate to humans. Methods: After development and validation, an e-mail survey was sent to all pediatricians and pediatric intensive care unit nurses and respiratory-therapists (RTs) affiliated with a Canadian University. We presented questions about demographics, methodology of AR, and expectations from AR. Responses of pediatricians and nurses/RTs were …

Do Zoos And Aquariums Promote Attitude Change In Visitors? A Critical Evaluation Of The American Zoo And Aquarium Study, Lori Marino, Scott O. Lilienfeld, Randy Malamud, Nathan Nobis, Ron Broglio

Lori Marino, PhD

Modern-day zoos and aquariums market themselves as places of education and conservation. A recent study conducted by the American Zoo and Aquarium Association (AZA) (Falk et al., 2007) is being widely heralded as the first direct evidence that visits to zoos and aquariums produce long-term positive effects on people’s attitudes toward other animals. In this paper, we address whether this conclusion is warranted by analyzing the study’s methodological soundness. We conclude that Falk et al. (2007) contains at least six major threats to methodological validity that undermine the authors’ conclusions. There remains no compelling evidence for the claim that zoos …

Do Zoos And Aquariums Promote Attitude Change In Visitors? A Critical Evaluation Of The American Zoo And Aquarium Study, Lori Marino, Scott O. Lilienfeld, Randy Malamud, Nathan Nobis, Ron Broglio

Nathan M. Nobis, PhD

Modern-day zoos and aquariums market themselves as places of education and conservation. A recent study conducted by the American Zoo and Aquarium Association (AZA) (Falk et al., 2007) is being widely heralded as the first direct evidence that visits to zoos and aquariums produce long-term positive effects on people’s attitudes toward other animals. In this paper, we address whether this conclusion is warranted by analyzing the study’s methodological soundness. We conclude that Falk et al. (2007) contains at least six major threats to methodological validity that undermine the authors’ conclusions. There remains no compelling evidence for the claim that zoos …

Addressing Distress And Pain In Animal Research: The Veterinary, Research, Societal, Regulatory And Ethical Contexts For Moving Forward, Kathleen Conlee, Martin Stephens, Andrew N. Rowan

Andrew N. Rowan, DPhil

While most people recognize that biomedical scientists are searching for knowledge that will improve the health of humans and animals, the image of someone deliberately causing harm to an animal in order to produce data that may lead to some future benefit has always prompted an uncomfortable reaction outside the laboratory. However, proponents of animal research have usually justified the practice by reference to greater benefits (new knowledge and medical treatments) over lesser costs (in animal suffering and death). Given that one of the costs of animal research is the suffering experienced by the animals, the goal of eliminating distress …

The Minimization Of Research Animal Distress And Pain: Conclusions And Recommendations, Kathleen Conlee, Martin Stephens, Andrew N. Rowan

Andrew N. Rowan, DPhil

While the attention given to preventing, assessing, and alleviating pain in research animals has increased noticeably in recent decades, much remains to be done both in terms of implementing best practices and conducting studies to answer outstanding questions. In contrast, the attention to distress (particularly non-pain induced distress) has shown no comparable increase. There are many reasons for this discrepancy, including the conceptual untidiness of the distress concept, the paucity of pharmacological treatments for distress, and perceived lack of regulatory emphasis on distress. These are challenges that need to be addressed and overcome. This book is intended to help meet …

Proof-Of-Concept Of Environmental Dna Tools For Atlantic Sturgeon Management, Jameson Hinkle

Theses and Dissertations

Abstract

The Atlantic Sturgeon (Acipenser oxyrinchus oxyrinchus, Mitchell) is an anadromous species that spawns in tidal freshwater rivers from Canada to Florida. Overfishing, river sedimentation and alteration of the river bottom have decreased Atlantic Sturgeon populations, and NOAA lists the species as endangered. Ecologists sometimes find it difficult to locate individuals of a species that is rare, endangered or invasive. The need for methods less invasive that can create more resolution of cryptic species presence is necessary. Environmental DNA (eDNA) is a non-invasive means of detecting rare, endangered, or invasive species by isolating nuclear or mitochondrial DNA (mtDNA) from the …

Dimethylsulfide Gas Transfer Coefficients From Algal Blooms In The Southern Ocean, T. G. Bell, Warren J. De Bruyn, Christa A. Marandino, S. D. Miller, C. S. Law, Eric S. Saltzman

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Air-sea dimethylsulfide (DMS) fluxes and bulk air-sea gradients were measured over the Southern Ocean in February-March 2012 during the Surface Ocean Aerosol Production (SOAP) study. The cruise encountered three distinct phytoplankton bloom regions, consisting of two blooms with moderate DMS levels, and a high biomass, dinoflagellate-dominated bloom with high seawater DMS levels (> 15 nM). Gas transfer coefficients were considerably scattered at wind speeds above 5 m s(-1). Bin averaging the data resulted in a linear relationship between wind speed and mean gas transfer velocity consistent with that previously observed. However, the wind-speed-binned gas transfer data distribution at all wind …