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Full-Text Articles in Organisms
Antimicrobial Activity Screening Of Recombinant And Synthetic Varasin A Defensin From The Hard Tick Dermacentor Variabilis Against Various Bacteria, Julia A. Sharp
Biological Sciences Theses & Dissertations
Hematophagous arthropods, such as ticks and mosquitoes, rely on their innate immune system for defense against pathogens ingested in a blood meal as well as those acquired through injury. In response to pathogen recognition, the production of antimicrobial peptides, such as defensin, is typically upregulated. Varisin, a defensin, is thought to be a key component in the immunocompetence of the hard tick D. variabilis against Borrelia burgdorferi, the causative agent of Lyme disease. To study the antimicrobial effects of varisin, recombinant varisin was expressed by both insect cells and E coli. Purification of the protein followed by enterokinase treatment yielded …
The Effect Of Mtad On Eight Strains Of E. Faecalis In Contaminated Root Canals, Bradley M. Newberry
The Effect Of Mtad On Eight Strains Of E. Faecalis In Contaminated Root Canals, Bradley M. Newberry
Loma Linda University Electronic Theses, Dissertations & Projects
The purpose of this investigation was to determine the antimicrobial effect of Biopure MTAD (a mixture of a tetracycline isomer, an acid, and a detergent) as a final irrigant on eight strains of Enterococcus faecalis (E. faecalis) in contaminated root canals of extracted human teeth. In addition, the Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) of MTAD were also measured on the same strains of bacteria. Two-hundred and forty extracted human teeth were instrumented using 1.3% NaOCl followed by 17% EDTA to remove the smear layer. The teeth were randomly contaminated with one of eight strains of E. …
Downstream Pathway Activation In Cultured Hepatocytes Following Treatment With Francisella Tularensis, Nicole Wakefield
Downstream Pathway Activation In Cultured Hepatocytes Following Treatment With Francisella Tularensis, Nicole Wakefield
Undergraduate Theses and Capstone Projects
Francisella tularensis, the intracellular pathogen that causes tularemia, was investigated to determine how it infects and replicates within mammalian hepatocytes. To date, it has been shown that hepatocytes can be infected by F. tularensis and that this causes considerable change in the protein phosphorylation state of several vital signaling molecules within the host cell. Protein kinase pathways can be mapped as signatures of infection. Hepatocytes tend to be susceptible to infection by F. tularensis, thus stimulating internal signaling. The Francisella strains used were selected with the goal of producing a model that can be used to elucidate the cell signaling …