Organisms Commons^™

Crispr/Cas9 In Yeast: A Multi-Week Laboratory Exercise For Undergraduate Students, Randi J. Ulbricht

Open Educational Resources

Providing undergraduate life-science students with a course-based research experience that utilizes cutting-edge technology, is tractable for students, and is manageable as an instructor is a challenge. Here, I describe a multi-week lesson plan for a laboratory-based course with the goal of editing the genome of budding yeast, Saccharomyces cerevisiae. Students apply knowledge regarding advanced topics such as: CRISPR/Cas9 gene editing, DNA repair, genetics, and cloning. The lesson requires students to master skills such as bioinformatics analysis, restriction enzyme digestion, ligation, basic microbiology skills, polymerase chain reaction, and plasmid purification. Instructors are led through the technical aspects of the protocols, …

The Voluntary Water Quality Monitoring Program : 1982 Project Report, Barbara Welch, Judy Potvin, Mary Ellen Dennis

Maine Collection

The Voluntary Water Quality Monitoring Program : 1982 Project Report

by Barbara Welch, Judy Potvin & Mary Ellen Dennis

Division of Environmental Evaluation and Lake Studies, Maine Department of Environmental Protection, Augusta, Maine 1982.

Contents: Understanding Maine's Lakes and Ponds / Acknowledgement / Temperature / Dissolved Oxygen / Other Measures of Lake Water Quality / Nutrients / Algae and Chlorophyll a / Transparency / Morphometry / Sampling / Protection / Classification / Answers to Most Frequently Asked Questions / Glossary of Terms / Appendix A : Maximum, Minimum, and Mean Values / Appendix B : Phosphate Content of Common Cleansers …