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Full-Text Articles in Organisms
Synthesis And In Vitro Characterization Of Novel Dextran- Methylprednisolone Conjugates With Peptide Linkers: Effects Of Linker Length On Hydrolytic And Enzymatic Release Of Methylprednisolone And Its Peptidyl Intermediates, Suman Penugonda, Anil Kumar, Hitesh K. Agarwal, Keykavous Parang, Reza Mehvar
Synthesis And In Vitro Characterization Of Novel Dextran- Methylprednisolone Conjugates With Peptide Linkers: Effects Of Linker Length On Hydrolytic And Enzymatic Release Of Methylprednisolone And Its Peptidyl Intermediates, Suman Penugonda, Anil Kumar, Hitesh K. Agarwal, Keykavous Parang, Reza Mehvar
Pharmacy Faculty Articles and Research
To control the rate of release of methylprednisolone (MP) in lysosomes, new dextran-MP conjugates with peptide linkers were synthesized and characterized. Methylprednisolone succinate (MPS) was attached to dextran 25 kDa using linkers with 1-5 Gly residues. The release characteristics of the conjugates in pH 4.0 and 7.4 buffers, blood, liver lysosomes, and various lysosomal proteinases were determined using a size-exclusion and/or a newly developed reversed-phase HPLC method capable of simultaneous quantitation of MP, MPS, and all five possible MPS-peptidyl intermediates. We synthesized conjugates with >= 90% purity and 6.9-9.5% (w/w) degree of MP substitution. The conjugates were stable at pH …