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Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Honors Theses
The overall goal of this project was to investigate the biological role of a putative metacaspase gene present in the mushroom fungus Schizophyllum commune. For this study, we have utilized a strain of S. commune that is unable to integrate DNA via the non-homologous end joining pathway. This forces transforming DNA to integrate homologously, as is required for the purposes of gene knockout. The gene Scp1 encodes a likely member of the metacaspase protein family, which are suspected to have activity similar to caspases, the latter crucial to programmed cell death. A knockout construct containing a non-functional version of Scp1 …