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The Effect Of Mismatch Primers On The Efficiency Of Amplification In Quantitative Polymerase Chain Reactions, Molly C. Dawkins
The Effect Of Mismatch Primers On The Efficiency Of Amplification In Quantitative Polymerase Chain Reactions, Molly C. Dawkins
Honors Undergraduate Theses
Polymerase chain reaction (PCR) is a method used in many research protocols to amplify a small amount of a short segment of DNA to millions of copies. PCR is used for many taxonomic studies, as well as for some medical diagnostic procedures. Through PCR, short DNA primers bind to the template DNA to allow the thermostable DNA polymerase to copy the DNA. Often, researchers create universal primers to target a conserved region of DNA in multiple species, for example, the 16S rRNA gene in bacteria. The problem with these universal primers is that they do not always perfectly match the …