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Articles 1 - 13 of 13
Full-Text Articles in Organisms
The Effects Of Light Intensity And Cell Structure On The Cultivation Of Arthrospira Platensis, Taylor Barnhart
The Effects Of Light Intensity And Cell Structure On The Cultivation Of Arthrospira Platensis, Taylor Barnhart
Honors Theses
As scientists explore further into space, more cost-effective resources are needed for long-term space travel. An interesting solution is Arthrospira platensis, a filamentous cyanobacteria high in proteins and nutrients, and known for its helical structure. In unfavorable conditions, coiled spirulina cells become straight. Spirulina converts carbon dioxide gas into pure oxygen and the different cell structures stimulate different responses in oxygen production and cultivation. In these experiments, 2.3 L containers of pure coiled spirulina and mixed (coiled and straight) spirulina were placed into 3 incubators with different light intensities: 51μmol/m2/s, 25μmol/m2/s, 12μmol/m2/s. Each experiment length was 72 hours and the …
Effects Of Red Light Intensity On Cultivation & Oxygen Production In Arthrospira Plantensis, Abigail Roberts
Effects Of Red Light Intensity On Cultivation & Oxygen Production In Arthrospira Plantensis, Abigail Roberts
Honors Theses
Maintaining life sustaining resources during long-term space travel has encouraged scientists to turn their attention to the potential benefits of a cyanobacteria named Arthrospira platensis, commonly known as Spirulina algae. This experiment measures the oxygen production and cell population growth of two structurally different Spirulina cultures under two different levels of red light intensities, 8 µmol/m 2Js (high); 3 µmol/m2/s (low). The cell population, oxygen produced, and oxygen produced per cell measurements were observed and recorded at three 24 hour intervals. It was found there was not a significant difference between high and low light intensities when considering the cell …
Effects Of Red Light Intensity On Cultivation And Oxygen Production In Arthrospira Platensis, Abigail Roberts
Effects Of Red Light Intensity On Cultivation And Oxygen Production In Arthrospira Platensis, Abigail Roberts
Honors Theses
Maintaining life sustaining resources during long-term space travel has encouraged scientists to turn their attention to the potential benefits of a cyanobacteria named Arthrospira platensis, commonly known as Spirulina algae. This experiment measures the oxygen production and cell population growth of two structurally different Spirulina cultures under two different levels of red light intensities, 8 µmol/m2/s (high); 3 µmol/m2/s (low). The cell population, oxygen produced, and oxygen produced per cell measurements were observed and recorded at three 24 hour intervals. It was found there was not a significant difference between high and low light intensities when considering the cell concentration …
Bacteriophages: Paving The Road For The Future Of Medicine, Luke Brinkerhoff
Bacteriophages: Paving The Road For The Future Of Medicine, Luke Brinkerhoff
Honors Theses
Bacteriophages are a possible solution to antibiotic resistance, which is predicted to be detrimental world-wide by the year 2050. Personal field research was also conducted for a project studying the characteristics of two bacteriophages on a single bacterial host.
Characterization Of A Plasmid-Based Dna Vaccine For Simian Immunodeficiency Virus, Priya Sanipara
Characterization Of A Plasmid-Based Dna Vaccine For Simian Immunodeficiency Virus, Priya Sanipara
Honors Theses
Described as one of the world’s worst pandemics, HIV (Human Immunodeficiency Virus) infects millions of people each year and is the cause for AIDS (Acquired Immunodeficiency Syndrome). Despite the development of vaccines for numerous infectious diseases such as polio, small pox, and influenza, a vaccine for HIV remains elusive due to the virus’s high mutation rate and ability to evade the immune system. HIV causes depletion of CD4+ lymphocytes, resulting in a weakened immune system. However, the development of a plasmid-based DNA vaccine approach may help revolutionize vaccine development for HIV due to its ability to confer cellular and humoral …
Testing Bacterial Antibiotic Production Under Carbohydrate And Protein Starvation, Briley Baird
Testing Bacterial Antibiotic Production Under Carbohydrate And Protein Starvation, Briley Baird
Honors Theses
Bacteria produce antibiotics when they are under stress, including starvation stress. Bacteria were tested under carbohydrate and protein starvation against Bacillus subtilis and Escherichia coli (due to the respective Gram positivity and negativity), in order to check for antibiotic production. The bacteria being tested were isolated by past Microbiology classes and stored in a -80°C freezer in the basement of Jones Science Center at Ouachita Baptist University. These test bacteria were grown on tryptic soy agar (TSA) to produce isolated bacterial colonies. Samples of isolated test colonies were then grown under conditions of carbohydrate starvation (M9 salts agar with 0.1 …
Organics: Exploring The Parallels Between Botany And Human Anatomy Through Sculpture And Watercolor Painting, Allison Borek
Organics: Exploring The Parallels Between Botany And Human Anatomy Through Sculpture And Watercolor Painting, Allison Borek
Honors Theses
Integrating my two passions of the visual arts and science for my senior thesis, I created a collection of paintings that represent human organs and the parallels between plant biology and animal biology. Genetically, plants are just as complex – if not more – than many animals. They have intricate sensory and regulatory systems highly comparable to humans, and on a cellular level, we share many analogous features. In addition, every single carbon atom that makes up the human body was at one time fixed in a plant during photosynthesis. We also rely on photosynthesis to give us the oxygen …
Comparison Of Wild And Cultivated Extracts Of Cordyceps Sinensis Apoptotic Potential, Katelyn Staring
Comparison Of Wild And Cultivated Extracts Of Cordyceps Sinensis Apoptotic Potential, Katelyn Staring
Honors Theses
Cordyceps sinensis is a mushroom which contains the compound cordycepin (3’-deoxyadenosine), an analogue of adenosine. In Traditional Chinese Medicine (TCM), cordycepin has multipurpose pharmacological uses including purported anti-tumor effects. In the present study, cordycepin was extracted from the wild mushroom as well as from various commercially available cultivated extracts. Previous research in this lab has demonstrated that cultivated extracts contain less cordycepin than the wild mushroom. However, it is unclear if the decrease in cordycepin correlates with decreased activity. To measure anti-tumor activity, extracts were used to treat human breast cancer cells (MCF-7 cells). In other labs, cordycepin has been …
Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Honors Theses
The overall goal of this project was to investigate the biological role of a putative metacaspase gene present in the mushroom fungus Schizophyllum commune. For this study, we have utilized a strain of S. commune that is unable to integrate DNA via the non-homologous end joining pathway. This forces transforming DNA to integrate homologously, as is required for the purposes of gene knockout. The gene Scp1 encodes a likely member of the metacaspase protein family, which are suspected to have activity similar to caspases, the latter crucial to programmed cell death. A knockout construct containing a non-functional version of Scp1 …
Molecular Evolution Of Insecticidal Spore-Forming Bacteria, John Pool
Molecular Evolution Of Insecticidal Spore-Forming Bacteria, John Pool
Honors Theses
Molecular methods are increasingly being used to determine the phylogeny of microorganisms. This research was intended to determine phylogenetic relationships for bacteria of the species Bacillus thuringiensis and other members of the Bacillus cereus group. Each strain was analyzed by its sasp-B gene sequence to determine its species classification and relation to other strains studied. Results of this study indicated that according to the sasp-B gene tree, the species Bacillus thuringiensis is a paraphyletic with respect to both Bacillus cereus and Bacillus anthracis. Some unexpected results and implications for species designations are also discussed.
Characterization Of Metal-Regulated Genes In Pseudomonas Fluorescens, Melanie Pearson
Characterization Of Metal-Regulated Genes In Pseudomonas Fluorescens, Melanie Pearson
Honors Theses
One side effect of today's industrialized world is increased levels of heavy metals in the environment. Many of these metals are necessary for biological function as trace elements, but at higher concentrations are toxic. Other metals, such as cadmium, are not beneficial at any level, and have only deleterious effects on living organisms. Cadmium is primarily thought to interfere with normal biological function of proteins. Human exposure to cadmium appears to primarily damage the kidneys, but may also affect the liver, lungs, immune system, and central nervous system. Bacteria, however, have developed several methods for handling toxic heavy metals: cation …
Experimentation To Develop Procedures To Be Used In The Investigation Of The Effects Of Restriction On The Unmodified Dna Of The Bacteriophage Lamda, John Haynes
Honors Theses
The results of experimentation approximately twenty years ago with variuos bacteriophages by Ralston and Krueger16, Anderson and Felix1, Luria and Human12, and by Bertani and Weigle2 have shown that the host range of a given phage depends directly on the bacterial strain on which the phage has last propagated. This event came to be known as host-induced modification or host-controlled variation. As this phage host range is controlled by the host environment and can be altered from one generation to another it has, according to Luria, made microbiology "the last stronghold of Lamarckism" …
The Role Of D-Gluconic Acid In The Regulation Of The Synthesis Of The Enzymes Of The Entner-Doudoroff Pathway In Pseudomonas Fluorescens, Steven C. Quay
The Role Of D-Gluconic Acid In The Regulation Of The Synthesis Of The Enzymes Of The Entner-Doudoroff Pathway In Pseudomonas Fluorescens, Steven C. Quay
Honors Theses
Induction of Entner-Duodoroff pathway enzymes in Pseudomonas fluorescens was investigated to study the role of gluconate as a possible inducer. Glucose oxidase-deficient mutants were isolated and characterized. One of these mutants, gox-7, was deficient in particulate glucose oxidase; another mutant, gox-17, was deficient in particulate glucose and gluconate oxidase activities. Gluconate, but not glucose, induced synthesis of gluconokinase and 6-phosphogluconate dehydratase in both mutants. High constitute levels of 2-keto-3-deoxy-6-phosphogluconate aldolase were found when both mutants were grown on glucose. Growth of parent and both mutant strains on glycerol also resulted in high levels of Entner-Doudoroff pathway enzymes. It was concluded …