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Full-Text Articles in Organisms

The Effects Of Light Intensity And Cell Structure On The Cultivation Of Arthrospira Platensis, Taylor Barnhart May 2023

The Effects Of Light Intensity And Cell Structure On The Cultivation Of Arthrospira Platensis, Taylor Barnhart

Honors Theses

As scientists explore further into space, more cost-effective resources are needed for long-term space travel. An interesting solution is Arthrospira platensis, a filamentous cyanobacteria high in proteins and nutrients, and known for its helical structure. In unfavorable conditions, coiled spirulina cells become straight. Spirulina converts carbon dioxide gas into pure oxygen and the different cell structures stimulate different responses in oxygen production and cultivation. In these experiments, 2.3 L containers of pure coiled spirulina and mixed (coiled and straight) spirulina were placed into 3 incubators with different light intensities: 51μmol/m2/s, 25μmol/m2/s, 12μmol/m2/s. Each experiment length was 72 hours and the …


Effects Of Red Light Intensity On Cultivation & Oxygen Production In Arthrospira Plantensis, Abigail Roberts Dec 2022

Effects Of Red Light Intensity On Cultivation & Oxygen Production In Arthrospira Plantensis, Abigail Roberts

Honors Theses

Maintaining life sustaining resources during long-term space travel has encouraged scientists to turn their attention to the potential benefits of a cyanobacteria named Arthrospira platensis, commonly known as Spirulina algae. This experiment measures the oxygen production and cell population growth of two structurally different Spirulina cultures under two different levels of red light intensities, 8 µmol/m 2Js (high); 3 µmol/m2/s (low). The cell population, oxygen produced, and oxygen produced per cell measurements were observed and recorded at three 24 hour intervals. It was found there was not a significant difference between high and low light intensities when considering the cell …


Effects Of Red Light Intensity On Cultivation And Oxygen Production In Arthrospira Platensis, Abigail Roberts Dec 2022

Effects Of Red Light Intensity On Cultivation And Oxygen Production In Arthrospira Platensis, Abigail Roberts

Honors Theses

Maintaining life sustaining resources during long-term space travel has encouraged scientists to turn their attention to the potential benefits of a cyanobacteria named Arthrospira platensis, commonly known as Spirulina algae. This experiment measures the oxygen production and cell population growth of two structurally different Spirulina cultures under two different levels of red light intensities, 8 µmol/m2/s (high); 3 µmol/m2/s (low). The cell population, oxygen produced, and oxygen produced per cell measurements were observed and recorded at three 24 hour intervals. It was found there was not a significant difference between high and low light intensities when considering the cell concentration …


Bacteriophages: Paving The Road For The Future Of Medicine, Luke Brinkerhoff May 2022

Bacteriophages: Paving The Road For The Future Of Medicine, Luke Brinkerhoff

Honors Theses

Bacteriophages are a possible solution to antibiotic resistance, which is predicted to be detrimental world-wide by the year 2050. Personal field research was also conducted for a project studying the characteristics of two bacteriophages on a single bacterial host.


Genomic Diversity Of Bacteriophages Infecting Microbacterium Spp, Deborah Jacobs-Sera, Nathan S. Reyna, Lawrence A. Abad, Richard M. Alvey, Kirk R. Anders, Haley G. Aull, Suparna S. Bhalla, Lawrence S. Blumer, David W. Bollivar, J. Alfred Bonilla, Kristen A. Butela, Roy J. Coomans, Steven G. Cresawn, Tom D'Elia, Arturo Diaz, Ashley M. Divens, Nicholas P. Edgington, Gregory D. Frederick, Maria D. Gainey, Rebecca A. Garlena, Kenneth W. Grant, Susan M.R. Gurney, Heather L. Hendrickson, Lee E. Hughes, Margaret A. Kenna, Karen K. Klyczek, Hari Kotturi, Travis N. Mavrich, Angela L. Mckinney, Evan C. Merkhofer, Jordan Moberg Parker, Sally D. Molloy, Denise L. Monti, Dana A. Pape-Zambito Jun 2020

Genomic Diversity Of Bacteriophages Infecting Microbacterium Spp, Deborah Jacobs-Sera, Nathan S. Reyna, Lawrence A. Abad, Richard M. Alvey, Kirk R. Anders, Haley G. Aull, Suparna S. Bhalla, Lawrence S. Blumer, David W. Bollivar, J. Alfred Bonilla, Kristen A. Butela, Roy J. Coomans, Steven G. Cresawn, Tom D'Elia, Arturo Diaz, Ashley M. Divens, Nicholas P. Edgington, Gregory D. Frederick, Maria D. Gainey, Rebecca A. Garlena, Kenneth W. Grant, Susan M.R. Gurney, Heather L. Hendrickson, Lee E. Hughes, Margaret A. Kenna, Karen K. Klyczek, Hari Kotturi, Travis N. Mavrich, Angela L. Mckinney, Evan C. Merkhofer, Jordan Moberg Parker, Sally D. Molloy, Denise L. Monti, Dana A. Pape-Zambito

Articles

The bacteriophage population is vast, dynamic, old, and genetically diverse. The genomics of phages that infect bacterial hosts in the phylum Actinobacteria show them to not only be diverse but also pervasively mosaic, and replete with genes of unknown function. To further explore this broad group of bacteriophages, we describe here the isolation and genomic characterization of 116 phages that infect Microbacterium spp. Most of the phages are lytic, and can be grouped into twelve clusters according to their overall relatedness; seven of the phages are singletons with no close relatives. Genome sizes vary from 17.3 kbp to 97.7 kbp, …


Testing Bacterial Antibiotic Production Under Carbohydrate And Protein Starvation, Briley Baird Jan 2018

Testing Bacterial Antibiotic Production Under Carbohydrate And Protein Starvation, Briley Baird

Honors Theses

Bacteria produce antibiotics when they are under stress, including starvation stress. Bacteria were tested under carbohydrate and protein starvation against Bacillus subtilis and Escherichia coli (due to the respective Gram positivity and negativity), in order to check for antibiotic production. The bacteria being tested were isolated by past Microbiology classes and stored in a -80°C freezer in the basement of Jones Science Center at Ouachita Baptist University. These test bacteria were grown on tryptic soy agar (TSA) to produce isolated bacterial colonies. Samples of isolated test colonies were then grown under conditions of carbohydrate starvation (M9 salts agar with 0.1 …


Genome Sequences Of Four Cluster P Mycobacteriophages, Erin L. Doyle, Christy L. Fillman, Nathan S. Reyna, Ruth C. Plymale, Deborah M. Tobiason, Daniel E. Westholm, Jonathan L. Askins, Brittany P. Backus, Ashlynn C. Baker, Harrison S. Ballard, Paul J. Bisesi, Logan Bond, Deanna Byrnes, Hannah Carlstedt, Kinnon S. Dodson, Megan J. Fallert, Kyla J. Foster, Daniel N. Games, Tristan R. Grams, Nancy A. Guild, Autumn Hurd, Nicholas Iwata, Cassidy R. Kepler, Lucinda R. Krenzke, Kelly Luekens, Jackie Lewis, Cali Mcentee, Justin C. Mcgee, Noah Nalley, Jade Prochaska, Reid G. Rogers, Jessica B. Schipper, Kelsey Snyder Jan 2018

Genome Sequences Of Four Cluster P Mycobacteriophages, Erin L. Doyle, Christy L. Fillman, Nathan S. Reyna, Ruth C. Plymale, Deborah M. Tobiason, Daniel E. Westholm, Jonathan L. Askins, Brittany P. Backus, Ashlynn C. Baker, Harrison S. Ballard, Paul J. Bisesi, Logan Bond, Deanna Byrnes, Hannah Carlstedt, Kinnon S. Dodson, Megan J. Fallert, Kyla J. Foster, Daniel N. Games, Tristan R. Grams, Nancy A. Guild, Autumn Hurd, Nicholas Iwata, Cassidy R. Kepler, Lucinda R. Krenzke, Kelly Luekens, Jackie Lewis, Cali Mcentee, Justin C. Mcgee, Noah Nalley, Jade Prochaska, Reid G. Rogers, Jessica B. Schipper, Kelsey Snyder

Articles

Four bacteriophages infecting Mycobacterium smegmatis mc2155 (three belonging to subcluster P1 and one belonging to subcluster P2) were isolated from soil and sequenced. All four phages are similar in the left arm of their genomes, but the P2 phage differs in the right arm. All four genomes contain features of temperate phages.


Comparative Genomics Of Cluster O Mycobacteriophages, Steven G. Cresawn, Nathan S. Reyna, Ruth Plymale, Welkin H. Pope, Deborah Jacobs-Sera, Charles A. Bowman, Daniel A. Russell, Rebekah M. Dedrick, Tamarah Adair, Kirk R. Anders, Sarah Ball, David Bollivar, Caroline Breitenberger, Sandra H. Burnett, Kristen Butela, Deanna Byrnes, Sarah Carzo, Kathleen A. Cornely, Trevor Cross, Richard L. Daniels, David Dunbar, Ann M. Findley, Chris R. Gissendanner, Urszula P. Golebiewska, Grant A. Hartzog, J. Robert Hatherill, Lee E. Hughes, Chernoh S. Jalloh, Carla De Los Santos, Kevin Ekanem, Sphindile L. Khambule, Rodney A. King, Christina King-Smith, Karen Klyczek, Greg P. Krukonis Mar 2015

Comparative Genomics Of Cluster O Mycobacteriophages, Steven G. Cresawn, Nathan S. Reyna, Ruth Plymale, Welkin H. Pope, Deborah Jacobs-Sera, Charles A. Bowman, Daniel A. Russell, Rebekah M. Dedrick, Tamarah Adair, Kirk R. Anders, Sarah Ball, David Bollivar, Caroline Breitenberger, Sandra H. Burnett, Kristen Butela, Deanna Byrnes, Sarah Carzo, Kathleen A. Cornely, Trevor Cross, Richard L. Daniels, David Dunbar, Ann M. Findley, Chris R. Gissendanner, Urszula P. Golebiewska, Grant A. Hartzog, J. Robert Hatherill, Lee E. Hughes, Chernoh S. Jalloh, Carla De Los Santos, Kevin Ekanem, Sphindile L. Khambule, Rodney A. King, Christina King-Smith, Karen Klyczek, Greg P. Krukonis

Articles

Mycobacteriophages - viruses of mycobacterial hosts - are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages - Corndog, Catdawg, Dylan, Firecracker, and YungJamal - designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O …


Emergent Fungal Entomopathogen Does Not Alter Density Dependence In A Viral Competitor, Andrew M. Liebhold, Ruth Plymale, Joseph S. Elkinton, Ann E. Hajek Jun 2013

Emergent Fungal Entomopathogen Does Not Alter Density Dependence In A Viral Competitor, Andrew M. Liebhold, Ruth Plymale, Joseph S. Elkinton, Ann E. Hajek

Articles

Population cycles in forest Lepidoptera often result from recurring density-dependent epizootics of entomopathogens. While these systems are typically dominated by a single pathogen species, insects are often infected by multiple pathogens, yet little is known how pathogens interact to affect host dynamics. The apparent invasion of northeastern North America by the fungal entomopathogen Entomophaga maimaiga some time prior to 1989 provides a unique opportunity to evaluate such interactions. Prior to the arrival of E. maimaga, the oscillatory dynamics of host gypsy moth, Lymantria dispar, populations were apparently driven by epizootics of a nucleopolyhedrovirus. Subsequent to its emergence, E. …